Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Aluminum Stress Inhibits Root Growth and Alters Physiological and Antioxidant Enzyme Responses in Alfalfa (Medicago sativa L.) Roots

알팔파 뿌리에 있어서 알루미늄 스트레스 처리에 따른 뿌리 생장 저해와 생리 및 항산화 반응의 변화

  • Min, Chang-Woo (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University) ;
  • Khan, Inam (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University) ;
  • Lee, Byung-Hyun (Division of Applied Life Sciences (BK21 Plus), IALS, Gyeongsang National University)
  • 민창우 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원) ;
  • 칸인앰 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원) ;
  • 이병현 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원)
  • Received : 2019.12.12
  • Accepted : 2019.12.15
  • Published : 2019.12.31
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Abstract

Acidic soil significantly reduces crop productivity mainly due to aluminum (Al) toxicity. Alfalfa (Medicago sativa L.) roots were exposed to aluminum stress (Al3+) in calcium chloride (CaCl2) solution (pH4.5) and root growth, physiological and antioxidant enzyme responses were investigated. The root growth (length) was significantly inhibited after 48 h of aluminum stress imposition. Histochemical staining with hematoxylin indicated significant accumulation of aluminum in Al stress-treated root tissues. Histochemical assay were also performed to detect superoxide anion, hydrogen peroxide and lipid peroxidation, which were found to be more in root tissues treated with higher aluminum concentrations. The enzymatic activity of CAT, POD and GR in root tissues was slightly increased after Al stress treatment. The result suggests that Al stress alters root growth in alfalfa and induces reactive oxygen species (ROS) production, and demonstrates that antioxidant enzymes involved in detoxification of Al-mediated oxidative stress.

Keywords

References

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