• 국제초고층학회논문집
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• 제5권3호
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• pp.233-241
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• 2016

This paper describes the structural solution for the design of a 29-story high-rise tower, which features a large office space above the Kabukiza Theatre. Kabuki is a type of Japanese traditional drama, and Kabukiza is the home building of Kabuki. GINZA KABUKIZA is the fifth generation of the Kabukiza Theatre, the first of which was built in 1889. In order to support 23 stories of office space above the theater - featuring a large void in plan - two 13-meter-deep mega-trusses, spanning 38.4 meters, are installed at the fifth floor of the building. Steelwork is used as a primary material for the structure above-ground, and a hybrid response control system using a buckling-restrained brace and oil damper is adopted in order to achieve a high seismic performance. This paper also describes the erection process of installing hydraulic jacks directly above the mega-truss at column bases, in order to keep the structure above the truss level during construction. The temple architecture of the previous Kabukiza is carefully restored by incorporating contemporary light-weight materials supported by steelwork.

• 한국해양환경ㆍ에너지학회지
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• 제10권3호
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• pp.173-180
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• 2007

파력에너지 변환을 위해 설계된 착저식 진동수주형 공기실 내의 파진폭 변화를 우리나라 연안의 특징적인 파랑 조건을 적용하여 조사하였다. 진동수주형 공기실의 형상인자가 파력에너지 변환에 미치는 영향을 파력에너지의 흡수 효율 관점에서 분석하였다. 실험적 및 수치적 접근법을 함께 사용하여 그 결과들을 상호 비교하였으며, 주어진 파랑 분포 하에서 파력에너지 흡수가 최대가 되는 형상인자들의 최적화를 목적으로 연구가 수행되었다. 실험은 공기실의 이차적원 형상을 가정하여 이차원 조파수조에서 실시되었으며, 수치적 해석은 공기실 내에서는 변동하는 공기압을 고려할 수 있도록 Rankine Green 함수를 적용하고 공기실 밖에서는 Kelvin Green 함수를 적용하는 합성 적분방정식에 기초하여 수행되었다. 공기실 상부의 덕트 직경, 공기실 폭, 공기실 전면벽 및 후면벽의 깊이는 흡수되는 파력에너지의 크기와 정점 주기를 민감하게 변화시키는 주요한 형상인자들임을 확인하였다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.381-384
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• 2008

본 연구에서는 폭렬방지용 합성섬유의 효과적인 투입방안 마련을 목적으로, 섬유혼입 프리믹스시멘트 결합재를 사용한 고강도 콘크리트의 폭렬방지 및 내화특성을 검토하였다. 내화시험 결과, 원주형 공시체의 폭렬은 W/C 15%의 수동투입에서 일부 박리 또는 파괴폭렬이 발생한 것을 제외하고 대부분 방지되었고, 전체적으로 섬유혼입 프리믹스시멘트는 폭렬방지 성능이 섬유 수동투입과 비교하여 동등 이상으로 우수하였으며, 결합재량이 많아 점성이 큰 고강도 콘크리트일수록 양호한 섬유의 분산성으로 폭렬방지에 더욱 효과적인 것으로 나타났다. 또한, 섬유혼입 프리믹스시멘트 결합재를 사용한 기둥부재의 온도이력은 폭렬이 방지되어 철근이 노출되지 않으면 최외곽 철근의 온도가 내화성능기준의 평균온도 538$^{\circ}C$를 넘지 않는 것으로 나타나, 고강도 콘크리트의 내화기준을 만족하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.144-149
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• 2001

For the production and purification of a single chain human insulin precursor, four types of fusion peptides $\beta$-galactosidase (LacZ), maltose binding protein (MBP), glutathione-S-transferase (GST), and (His)(sub)6-tagged sequence (HTS) were investigated. Recombinant E. coli harboring hybrid genes was cultivated at 37$\^{C}$ for 1h, and gene induction occurred when 0.2mM of isopropyl-D-thiogalactoside (IPTG) was added to the culture broth, except for E. coli BL21 (DE3) pLysS harboring a pET-BA cultivation with 1.0mM IPTG, followed by a longer than 4h batch fermentation respectively. DEAE-Sphacel and Sephadex G-200 gel filtration chromatography, amylose affinity chromatography, glutathione-sepharose 4B affinity chromatography, and a nickel chelating affinity chromatography system as a kind of immobilized metal ion affinity chromatography (IMAC) were all employed for the purification of a single chain human insulin precursor. The recovery yields of the HTS-fused, GST-fused, MBP-fused, and LacZ-fused single chain human insulin precursors resulted in 47%, 20%, 20%, and 18% as the total protein amounts respectively. These results show that a higher recovery yield of the finally purified recombinant peptides was achieved when affinity column chromatography was employed and when the fused peptide had a smaller molecular weight. In addition the pET expression system gave the highest productivity of a fused insulin precursor due to a two-step regulation of the gene expression, and the HTS-fused system provided the highest recovery of a fused insulin precursor based on a simple and specific separation using the IMAC technique.

• 한국지진공학회논문집
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• 제26권2호
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• pp.71-82
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• 2022

This paper presents the effect on the inelastic behavior and structural performance of concrete and filled steel pipe through a numerical method for reliable judgment under various load conditions of the CJS composite structural system. Variable values optimized for the CJS synthetic structural system and the effects of multiple variables used for finite element analysis to present analytical modeling were compared and analyzed with experimental results. The Winfrith concrete model was used as a concrete material model that describes the confinement effect well, and the concrete structure was modeled with solid elements. Through geometric analysis of shell and solid elements, rectangular steel pipe columns and steel elements were modeled as shell elements. In addition, the slip behavior of the joint between the concrete column and the rectangular steel pipe was described using the Surface-to-Surface function. After finite element analysis modeling, simulation was performed for cyclic loading after assuming that the lower part of the foundation was a pin in the same way as in the experiment. The analysis model was verified by comparing the calculated analysis results with the experimental results, focusing on initial stiffness, maximum strength, and energy dissipation capability.

• Biomolecules & Therapeutics
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• 제32권3호
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• pp.379-389
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• 2024

This study was aimed to evaluate endogenous metabolic changes before and after cisplatin and radiation therapy in patients with cervical cancer via untargeted metabolomic analysis using plasma samples. A total of 13 cervical cancer patients were enrolled in this study. Plasma samples were collected from each patient on two occasions: approximately one week before therapy (P1) and after completion of cisplatin and radiation therapy (P2). Of the 13 patients, 12 patients received both cisplatin and radiation therapy, whereas one patient received radiation therapy alone. The samples were analyzed using the Ultimate 3000 coupled with Q Exactive^TM Focus Hybrid Quadrupole-Orbitrap^TM mass spectrometry (Thermo Fisher Scientific, Waltham, MA, USA). Chromatographic separation utilized a Kinetex C18 column 2.1×100 mm (2.6 ㎛) (Phenomenex, Torrance, CA, USA), and the temperature was maintained at 40℃. Following P2, there were statistically significant increases in the concentrations of indoxyl sulfate, phenylacetylglutamine, Lysophosphatidyethanolamine (LysoPE) (18:1), and indole-3-acetic acid compared with the concentrations observed at P1. Specifically, in the human papillomavirus (HPV) noninfection group, indoxyl sulfate, LysoPE (18:1), and phenylacetylglutamine showed statistically significant increases at P2 compared with P1. No significant changes in metabolite concentrations were observed in the HPV infection group. Indoxyl sulfate, LysoPE (18:1), phenylacetylglutamine, and indole-3-acetic acid were significantly increased following cisplatin and radiation therapy.

• Steel and Composite Structures
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• 제52권4호
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• pp.405-418
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• 2024

To study the influence of different reduced beam section (RBS) on the mechanical performance of modular boltedwelded hybrid connection joints (MHCJs), this article uses ABAQUS to establish and verify the finite element model (FEM) of the test specimens on the basis of quasi-static test research. Based on, 14 joint models featuring different RBS are devised to evaluate their influence on seismic behavior, such as joint failure mode, bending moment (M)-rotation angle (θ) curve, ductility, and energy consumption. The results indicate that when the flange and web are individually weakened, they alleviate to some extent the concentrated stress of the core module (CM) and column end steel skeleton in the joint core area, but both increase the stress on the flange connecting plate (FCP). At the same time, the impact of both on seismic performance such as bearing capacity, stiffness, and energy consumption is relatively small. When simultaneously weakening the flange and web of the steel beam, forming plastic hinges at the weakened position of the beam end, significantly alleviated the stress concentration of the CM and the damage at the FCP, improving the overall deformation and energy consumption capacity of joints. But as the weakening size of the web increases, the overall bearing capacity of the joint shows a decreasing trend.

• 생태와환경
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• 제39권2호통권116호
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• pp.167-177
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• 2006

도암호는 송천의 상류수계에 건설된 호수임에도 불구하고 담수초기부터 주변에 산재해 있는 비점오염원의 영향으로 연중 탁도농도가 높았다. 도암호의 수질은 조사기간동안 투명도가 평균 0.8m이었으며, Chl. a는 표층 평균(0 ${\sim}$ 5 m)이 18.5 ${\mu}g\;L^{-1}$, 총인은 평균 111 ${\mu}g\;L^{-1}$, 총질소는 평균 4.1 mg $L^{-1}$로 나타났다. 이는 U.S. EPA (1976)에서 제시한 부영양화 정도를 초과하는 수준이었으나, 상류에서 유입된 오염원이 댐 하류지점에서 낮게 나타났다. 도암호의 어류상은 총 6과 26종 9,600개체가 채집되었으며, 우점종으로는 돌고기 (34.6%), 아우걸종으로는 피라미 (22.5%)로 나타났다. 또한 수환경에 따른 어류상을 살펴 본 결과 도암호 상류에서는 유영성어종의 비율이 높게 나타났으며, 하류로 갈수록 저서성 어종의 비율이 높게 출현하였다. 이는 도암호의 상류와 하류의 하상구조에 따른 차이와 부유물질 및 탁도와 같은 수진의 상 ${\cdot}$ 하류간의 차이에 의한 영향으로 사료된다. 그러므로 도암호는 송천수계에서 오염을 감소시키는 완충지대의 역활을 하고 있는 것으로 사료된다. 한편 특이할 사항은 Comat (C. auratus의 품종: Gold fish)과 붕어 (C. auratus)치 사이에서 교잡된 붕어 (C. auratus. Hybrid type)가 출현한 것이다.

• Steel and Composite Structures
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• 제34권5호
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• pp.743-767
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• 2020

Due to the impressive flexural performance, enhanced compressive strength and more constrained crack propagation, Fibre-reinforced concrete (FRC) have been widely employed in the construction application. Majority of experimental studies have focused on the seismic behavior of FRC columns. Based on the valid experimental data obtained from the previous studies, the current study has evaluated the seismic response and compressive strength of FRC rectangular columns while following hybrid metaheuristic techniques. Due to the non-linearity of seismic data, Adaptive neuro-fuzzy inference system (ANFIS) has been incorporated with metaheuristic algorithms. 317 different datasets from FRC column tests has been applied as one database in order to determine the most influential factor on the ultimate strengths of FRC rectangular columns subjected to the simulated seismic loading. ANFIS has been used with the incorporation of Particle Swarm Optimization (PSO) and Genetic algorithm (GA). For the analysis of the attained results, Extreme learning machine (ELM) as an authentic prediction method has been concurrently used. The variable selection procedure is to choose the most dominant parameters affecting the ultimate strengths of FRC rectangular columns subjected to simulated seismic loading. Accordingly, the results have shown that ANFIS-PSO has successfully predicted the seismic lateral load with R² = 0.857 and 0.902 for the test and train phase, respectively, nominated as the lateral load prediction estimator. On the other hand, in case of compressive strength prediction, ELM is to predict the compressive strength with R² = 0.657 and 0.862 for test and train phase, respectively. The results have shown that the seismic lateral force trend is more predictable than the compressive strength of FRC rectangular columns, in which the best results belong to the lateral force prediction. Compressive strength prediction has illustrated a significant deviation above 40 Mpa which could be related to the considerable non-linearity and possible empirical shortcomings. Finally, employing ANFIS-GA and ANFIS-PSO techniques to evaluate the seismic response of FRC are a promising reliable approach to be replaced for high cost and time-consuming experimental tests.

• 한국지진공학회논문집
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• 제6권3호
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• pp.23-29
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• 2002

상부 벽식, 하부 골조로 이루어진 주상복합 건물은 주어진 대지 내에 여러 기능을 수용하기 위한 건물의 구조로서 대도시에서는 광범위하게 사용되고 있다. 그러나 이러한 전이보 시스템은 구조시스템의 수직적 불연속성으로 인하여 많은 문제점을 가지고 있다. 본 연구에서는 현재 일반적으로 주상복합건물에 사용중인 춤이 깊은 전이보를 가진 구조시스템을 조사하고 아치 시스템으로 대체하기 위하여 기존의 전이보를 사용하였을 경우 발생하는 아치거동의 형태를 규정하고 이를 아치의 형태로 나타내어 전이보 시스템을 대체하였다. 전이보 시스템을 대체한 보다 효율적인 아치 시스템에 대한 여러 가지 고찰을 통해 아치 시스템의 적용성 여부를 판단하고, 이 시스템과 기존의 전이보 시스템을 중력방향 하중과 횡 방향 하중에 대하여 비교하였다. 전이보 시스템과 아치 시스템을 중력방향 하중과 횡 방향 하중에 대해 비교한 결과, 전이보 시스템이 가지고 있는 구조적인 문제점을 많이 감소시킬 수 있었다. 전이보 시스템을 대체할 아치 시스템은 아치 작용으로 인해 발생하는 전이보의 전단 거동에 대한 불확실성, 전이보 자체의 큰 강성으로 인하여 기둥 부분에 발생하는 추가 모멘트 벽 하단부에 발생하는 전단 응력 집중 현상 및 벽 하부 중앙과 전이보 중앙부의 비효율성 등과 같은 문제점을 감소시킬 수 있는 효율적인 시스템으로 판단된다.