• Journal of Microbiology and Biotechnology
• /
• v.11 no.6
• /
• pp.1115-1119
• /
• 2001

Eight Escherichia coli cells with aerobic growth deflects were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created transcriptional fusion to lacZY. Two of these mutants, CLIO and CLl2, were irradiated with UV to obtain specialized transducing phages. The phages that took out the neighboring chromosomal DNA of the related gene responsible for deflective aerobic growth were identified. The in vivo cloned chromosomal sequence revealed that the mutated gene of CLIO was located at min 34.5 on the Escherichia coli linkage map and 1,599,515 on the physical map. The physical map indicated that there were 7 cistrons in the operon. We named this operon oxg10. The promoter sequence of oxg10 exhibited a possible binding site far SoxS, a transcriptional regulator that activates the transcription of various SoxRS regulon genes. Transferring the oxg10:: ${\lambda}placMu53$ mutation into the wild-type strain, RZ4500, resulted in the inhibition of normal aerobic growth, while the salute mutation in strain MO inhibited aerobic cell growth completely. The full operon sequences of oxg10 were cloned from the Excherichia coli genomic library. The mutated gene of CLl2 was identified to be a sucA gene encoding the ${\alpha}$-ketoglutarate dehydrogenase El component in the TCA cycle.

• Journal of Korea Multimedia Society
• /
• v.13 no.11
• /
• pp.1657-1666
• /
• 2010

Underwater wireless sensor networks (UWSN) need stable efficient data transmission methods because of environmental characteristics such as limited energy resource, limited communication bandwidth, variable propagation delay and so on. In this paper, we explain an enhanced hybrid transmission method that uses a hexagon tessellation with an ideal cell size in a two-dimensional underwater wireless sensor network model (2D) that consists of fixed position sensors on the bottom of the ocean. We also propose an energy efficient sensing and communication coverage method for effective data transmission in a three-dimensional underwater wireless sensor network model (3D) that equips anchored sensors on the bottom of the ocean. Our simulation results show that proposed methods are more energy efficient than the existing methods for each model.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.9
• /
• pp.1237-1241
• /
• 2005

Mannose-P-dolichol utilization defect 1 (MPDU1) gene is required for utilization of the mannose donor MPD in synthesis of both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols (GPI) which are important for functions such as protein folding and membrane anchoring. The full length cDNA of the porcine MPDU1 was determined by in silico cloning and rapid amplification of cDNA ends (RACE). The deduced amino acid showed 91% identity to the corresponding human sequence with five predicted transmembrane regions. RT-PCR was performed to detect its expression pattern in five tissues and results showed that it is expressed ubiquitously among the tissues checked. A single nucleotide substitution resulting in the amino acid change (137 Tyr-137 His) was detected within exon 5. Allele frequencies in six pig breeds showed distinctive differences between those Chinese indigenous pigs breeds and European pigs. Using the pig/rodent somatic cell hybrid panel (SCHP), we mapped the porcine MPDU1 gene to SSC12, which is consistent with the comparative mapping result as conservative syntenic groups presented between human chromosome 17 and pig chromosome 12.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.12
• /
• pp.3817-3824
• /
• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.13 no.2
• /
• pp.169-174
• /
• 2003

The trial and success of malicious cyber attacks has been increased rapidly with spreading of Internet and the activation of a internet shopping mall and the supply of an online, or an offline internet, so it is expected to make a problem more and more. The goal of intrusion detection is to identify unauthorized use, misuse, and abuse of computer systems by both system insiders and external penetrators in real time. In fact, the general security system based on Internet couldn't cope with the attack properly, if ever. other regular systems have depended on common vaccine softwares to cope with the attack. But in this paper, we will use the positive selection and negative selection mechanism of T-cell, which is the biologically distributed autonomous system, to develop the self/nonself recognition algorithm and AIS (Artificial Immune System) that is easy to be concrete on the artificial system. For making it come true, we will apply AIS to the network environment, which is a computer security system.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.1
• /
• pp.205-212
• /
• 2010

This paper describes a core generator (FCore_Gen) which generates Verilog-HDL models of 640 different FFT/IFFT cores with selected parameter value for OFDM-based communication systems. The generated FFT/IFFT cores are based on in-place single memory architecture and use a hybrid structure of radix-4 and radix-2 DIF algorithm to accommodate various FFT lengths. To achieve both memory reduction and the improved SQNR, a conditional scaling technique is adopted, which conditionally scales the intermediate results of each computational stage. The cores synthesized with a $0.35-{\mu}m$ CMOS standard cell library can operate with 75-MHz@3.3-V, and a 8,192-point FFT can be computed m $762.7-{\mu}s$, thus the cores satisfy the specifications of wireless LAN, DMB, and DVB systems.

• Asian Journal of Atmospheric Environment
• /
• v.12 no.1
• /
• pp.78-86
• /
• 2018

Filter-based sampling techniques are the conventional way to collect particulate matter, but particles collected and entangled in the filter fibers are difficult to be removed and thus not suited for the following cell- and animal-based exposure experiments. Collecting aerosol particles in powder form using a cyclone instead of a filter would be a possible way to solve this problem. We developed a hybrid virtual-impactor/cyclone high-volume fine and coarse particle sampler and assessed its performance. The developed system achieved 50% collection efficiency with components having the following aerodynamic cut-off diameters: virtual impactor, $2.4{\mu}m$; fine-particle cyclone, $0.18-0.30{\mu}m$; and coarse-particle cyclone, $0.7{\mu}m$. The virtual impactor used in our set-up had good $PM_{2.5}$ separation performance, comparable to that reported for a conventional real impactor. The newly developed sampler can collect fine and coarse particles simultaneously, in combination with exposure testing with collected fine- and coarse-particulate matter samples, should help researchers to elucidate the mechanism by which airborne particles result in adverse health effect in detail.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.5
• /
• pp.807-812
• /
• 2002

In order to analyze the cellular proteins which interact with core protein of hepatitis C virus (HCV), a yeast two-hybrid screening technique was employed. A carboxyl terminus truncated core protein, which contained amino acid residues from the 1st to 120th, was used as a bait to screen cellular proteins. The expression library prepared from HeLa cell was screened and 400 positive clones were selected. The 75 clones from the positive clones were sequenced and analyzed by undergoing the Blast search. Interestingly, 7 out of the 75 clones encoded E7 antigen of human papilloma virus (HPV). We studied in detail the Interaction between the truncated version of HCV core and E7 antigen in vitro. The core$_{120}$ protein expressed in chimeric form with G57 was able to bring down the E7 protein of HPV type 18 expressed in bacteria. It is therefore suggested that the core of HCV might affect the interaction between E7 and a normal cellular tumor suppressor, known as Rb protein.

• 제어로봇시스템학회:학술대회논문집
• /
• 2000.10a
• /
• pp.103-103
• /
• 2000

In this paper to cope with the reduction of products life-cycle as the variety of products along with the various demands of consumers, a virtual simulator is developed to make the changeover of manufacturing line efficient to embody a virtual simulation similar to a real manufacturing line. The developed virtual simulator can design a layout of a factory and make the time scheduling. Every factory has one simulator so that one product can be manufactured in the factories to use them as virtual factories. We suggest a scheme that heightens the agility to the diversity of manufacturing models by making the information of manufacturing lines and products models to be shared. The developed unit simulator can construct a proper virtual manufacturing line along with the required process of products using several kinds of operator and work cell. A user with the simulator can utilize an interface that makes one to manage the separate task process for each manu(acturing module, change operator components and work cells, and easily teach tasks of each task module. The developed simulator was made for users convenience by Microsoft Visual C++ 6.0 that can develop a program supplying graphic user interface environment and by OpenGL of the Silicon Graphics as a graphic library to embody 3D graphic environment. Also, we show that the simulator can be used efficiently for the agile manufacturing by the communication among the factories being linked by TCP/IP and a hybrid database system made by a hierarchical model and a relational model being developed to standardize the data information.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.35 no.2A
• /
• pp.209-215
• /
• 2010

This paper describes a design of variable-length FFT/IFFT processor (VL_FCore) for OFDM-based multi-standard communication systems. The VL_FCore adopts in-place single-memory architecture, and uses a hybrid structure of radix-4 and radix-2 DIF algorithms to accommodate various FFT lengths in the range of $N=64{\times}2^k\;(0{\leq}k{\leq}7)$. To achieve both memory size reduction and the improved SQNR, a two-step conditional scaling technique is devised, which conditionally scales the intermediate results of each computational stage. The performance analysis results show that the average SQNR's of 64~8,192-point FFT's are over 60-dB. The VL_FCore synthesized with a $0.35-{\mu}m$ CMOS cell library has 23,000 gates and 32 Kbytes memory, and it can operate with 75-MHz@3.3-V clock. The 64-point and 8,192-point FFT's can be computed in $2.25-{\mu}s$ and $762.7-{\mu}s$, respectively, thus it satisfies the specifications of various OFDM-based systems.