• BMB Reports
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• 제34권6호
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• pp.526-530
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• 2001

The translationally controlled tumor protein (TCTP), also known as the IgE-dependent histamine releasing factor (HRF), was used in the yeast two-hybrid system to screen the interacting molecules. We obtained the N-terminus truncated rat fast myosin alkai light chain from the rat skeletal muscle cDNA library in the screening. Since either TCTP/HRF or the myosin light chain is known to be associated with histamine secretion from RBL-2H3 cells, we investigated the possible interaction between rat TCTP/HRF and nonmuscle myosin light chain in these cells. We used affinity chromatography and coimmunoprecipitation. Our data suggests that HRF and the myosin light chain interact, which may play an important role in histamine release in RBL-2H3 cells.

• BMB Reports
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• 제33권1호
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• pp.59-62
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• 2000

Hepatitis C virus (HCV) nonstructural 5B (NS5B) protein is an RNA-dependent RNA polymerase (RdRp). To determine whether it can contribute to viral replication by interaction with cellular proteins, the yeast two-hybrid screening system was employed to screen a human liver cDNA library. Using the HCV NS5B as a bait, we have isolated positive clones encoding a cellular protein. The NS5B interacting protein, 5BIP, is a novel cellular protein of 170 amino acids. Interaction of the HCV NS5B protein with 5BIP was confirmed by a protein-protein blotting assay. Recently, we have demonstrated that NS5B possesses an RdRp activity and thus it is possible that 5BIP, in association with NS5B, plays a role in HCV replication.

• Plant Biotechnology Reports
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• 제3권2호
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• pp.127-134
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• 2009

Members of the NAM-ATAF-CUC (NAC) protein family are plant-specific transcription factors that contain a highly conserved N-terminal NAC-domain and diverse C-terminal regions. They have been implicated in plant development and abiotic stress responses. To identify interacters of rice NAC-domain proteins (OsNACs), we performed yeast two-hybrid screening of rice cDNA library using OsNAC5 as a bait, and the results showed that OsNAC5 interacts with other OsNACs including itself. To delineate an interacting domain, a series of deletion constructs of four OsNACs were made and transformed into yeast in various combinations. The results revealed that the conserved NAC domain of OsNACs plays a primary role in homodimer and heterodimer formation, and a part of C-terminal sequence is also necessary for the interaction. In vitro pull-down assays using recombinant OsNAC proteins verified the dimer formations, together suggesting that OsNACs may act by forming homodimers and/or heterodimers in plants.

• 한국정보통신학회논문지
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• 제14권12호
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• pp.2675-2680
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• 2010

3D 그래픽을 처리하는 연산 과정에는 고정적인 연산만을 수행하는 영역과 Shader 등과 같은 명령어에 의한 프로그래밍이 요구되는 영역이 구분되어 있다. 이러한 3D 파이프라인의 특성을 고려하여 fixed 구조로 설계한 graphics hardware와 명령어 기반의 programmable hardware를 혼합한 구조로 설계하면 효율적인 그래픽 처리가 가능하다. 본 논문에서는 이러한 혼합 구조에 적합한 OpenGL ES(Open Graphics Library Embedded System) 2.0을 지원하는 Fragment Shader를 설계하였다. fixed hardware와 Shader간 데이터 입출력으로 인해 발생할 수 있는 전체 파이프라인의 지연을 줄일 수 있도록 내부 인터페이스를 최적화하였으며 Shader 내부 레지스터 그룹을 interleaved 구조로 설계하여 레지스터 면적과 처리 속도를 개선하였다.

• 한국도서관정보학회지
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• 제39권3호
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• pp.299-320
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• 2008

이 연구는 MRF(Master Reference File)를 바탕으로 2005년에 영국의 BSI(British Standards Institution) 에서 인쇄 형태로 출판한 UDC(Universal Decimal Classification)의 표준판(Standard edition)을 대상으로 그 성립배경과 구조적 특성을 분석하고, 한국어 간략판과 비교하였다. UDC는 다른 십진식분류표에 대비하여 독특한 구조적 특성이 있으며, 한국어 간략판과 비교하여 볼 때 국제적 보편성을 더욱 강화하였고, 보조표를 통한 조합의 방식이 더욱 확대되었다. 이 연구를 바탕으로 앞으로의 KDC(Korean Decimal Classification)의 개정에 있어서 참고가 될 만한 KDC MRF 데이터베이스 개발과 보조표의 개선과 새로운 신설을 제언하였다.

• BMB Reports
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• 제42권11호
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• pp.737-742
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• 2009

Hypoxia-inducible factor-$1{\alpha}/{\beta}$ (HIF-$1{\alpha}/{\beta}$) is a heterodimeric transcriptional activator that mediates gene expression in response to hypoxia. HIF-$1{\alpha}$ has been noted as an effective therapeutic target for ischemic diseases such as myocardiac infarction, stroke and cancer. By using a yeast two-hybrid system and a random peptide library, we found a 16-mer peptide named F29 that directly interacts with the bHLH-PAS domain of HIF-$1{\alpha}$. We found that F29 facilitates the interaction of the HIF-$1{\alpha/\beta}$ heterodimer with its target DNA sequence, hypoxia-responsive element (HRE). The transient transfection of an F29-expressing plasmid increases the expression of both an HRE-driven luciferase gene and the endogenous HIF-1 target gene, vascular endothelial growth factor (VEGF). Taken together, we conclude that F29 increases the DNA-binding ability of HIF-$1{\alpha}$, leading to increased expression of its target gene VEGF. Our results suggest that F29 can be a lead compound that directly targets HIF-$1{\alpha}$ and increases its activity.

• 한국실내디자인학회논문집
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• 제20권2호
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• pp.47-54
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• 2011

In Alvar Aalto's designs, important factors of complex building designs including Wolfsburg cultural center are that they insinuate how to develop each architectural type and how to combine them in a building. The humane and physical background studies, and wholistic and systematic approaches are adopted to fulfill research purposes. Comparison with other buildings are necessary to reveal true meaning of this building. The result of analysis show characteristics of Wolfsburg cultural center as follows; hybrid composition of mass and elevation, spatial effect using level difference and light, massing variation of roof, and creating space for various activities. Wolfsburg cultural center designed in late 1950s has greatly affected Aalto's later works through various architectural experiments because it is the first cultural complex project that combined various architectural types. Especially library in the cultural center has shown transitional characteristics to famous fan-type libraries of 1960s while maintained features about Viipuri library. Wolfsburg cultural center act as an another type which present new principles of clustering, massing and exterior design. Its true meaning lies in forming a humanizing place beyond spatial configuration.

• 정보관리학회지
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• 제30권3호
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• pp.249-271
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• 2013

우리나라 의학도서관의 인쇄본 학술지 공동보존체제를 구축하기 위하여, 선행 연구와 사례를 통해 공동보존의 유형과 발전현황을 고찰하고, 한국의학도서관협의회 회원도서관들을 대상으로 인쇄본 학술지 폐기 실태와 공간현황 및 공동보존 요구도와 참여의지 및 선호방식 등을 조사하였다. 분석 결과에 기초하여, 기존의 MEDLIS 인프라를 활용한 집중형과 분산형으로 구성된 하이브리드 모형을 제안하였으며, 기본사항과 세부내용, 단계별 시행추진 전략을 제시하였다. 마지막으로 성공적인 구현을 위한 한국의 학도서관협의회의 노력을 제언하였다.

• 정보관리연구
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• 제38권1호
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• pp.99-119
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• 2007

사서직의 업무는 도서관의 패러다임이 전환됨에 따라 그 성격이 함께 변한다고 할 수 있다. 즉 전통적인 정보자료 관리중심의 패러다임에서 소장과 접근의 조화를 강조하는 하이브리드형의 절충형 패러다임으로 변하고 있다. 전문도서관의 블루오션을 창출하기 위하여 내 외부적인 환경변화를 SWOT기법으로 분석하고, 합리적인 도서관 운영을 위하여 ERCR 분석을 하였다. 사서업무 영역의 다변화를 조사하기 위하여 대덕연구단지 전문도서관에 근무하는 사서를 대상으로 하였으며, 문헌조사도 병행하였다.

• 제어로봇시스템학회논문지
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• 제19권12호
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• pp.1125-1131
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• 2013

Nowadays, embedded software development using the MATLAB/Simulink system is gradually emerging. Studies generating the parts of embedded S/W in a Rapid Prototype are presented. In this paper, a method to generate the entire embedded S/W of enhanced AC motor control is proposed. High performance motor control could not be achieved with the basic Simulink library and RAppID Toolbox library as it does not have PWM based Interrupt, an ASAC (Analog Sensing for AC Motors) function and other special functions of the Freescale MPC555x. Consequently, the required libraries for enhanced AC motor control are created by Legacy code tool, TLC (Target Language Compiler) and S-Function (System-Function) of MATLAB/ Simulink and utilized in the Rapid Prototype. Motor control performance and execution time are compared automatically to the generated-code S/W with the hand coded S/W. The IPMSM (Interior Permanent Magnet Synchronous Motor) and MPC5553 board that were designed as the AC motor controller for hybrid electrical vehicle are used for the test. The performances meet the requirements and satisfactory results are acquired.