• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.5
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• pp.716-724
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• 2015

Trienzyme extraction coupled with microplate assay (Lactobacillus casei subsp. rhamnosus) was validated and applied for the determination of folate (vitamin B9) in Korean ancestral rite foods. Foods included five Guk (Tang), eleven Sookchaes, eight Jeoks, nine Jeons, six Jjims, and twenty desserts. Folate was detected in all samples: Guk (Tang) 4.62~18.84, Sookchae 6.13~48.40, Jeok 5.49~49.50, Jeon 6.96~30.77, Jjim 10.34~38.88, and desserts $3.33{\sim}49.55{\mu}g/100g$. The lowest folate content was observed in Sikhye ($3.33{\mu}g/100g$), whereas the highest was observed in Songhwa-dasik ($49.55{\mu}g/100g$). Folate analyses of certified reference materials, BCR-121 (whole meal flour) and BCR-487 (pig liver), showed good recoveries of 90.0% (0.45 mg/kg) and 92.4% (12.3 mg/kg), respectively. The recoveries (96.0 to 106.2%) obtained by analyzing eight spiked samples with different matrices also showed good accuracy. Both repeatability and reproducibility were less than 5%, indicating good precision. The quality control chart (n>30) obtained by running commercial folate fortified-wheat flour once a week for about 10 months showed that all assays were under control. All validation method and analytical quality control results showed that folate contents in Korean ancestral rite foods produced by microplate assay were reliable enough to be used for the construction of a national folate database.

• Economic and Environmental Geology
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• v.38 no.3 s.172
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• pp.305-317
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• 2005

This study addresses the preliminary results of rock slope stability analyses including hazard assessments for slope failure conducted on the selected sections of rural road cut slope which are about 4 km long. The study area is located in the Mt. Chuntae northeast of Busan and mainly composed of Cretaceous rhyolitic ash-flow tuff', fallout tuff, rhyolitc and andesite. The volcanic rock mass in the area has a number of discontinuities that produce a potentially unstable slope, as the present cut slope is more than 70 degrees in most of the slope sections. Discontinuity geometry data were collected at selected 8 scanline sections and analyzed to estimate important discontinuity geometry parameters to perform rock slope kinematic and block theory analyses. Kinematic analysis for plane sliding has resulted in maximum safe slope angles greater than $65^{\circ}$ for most of the discontinuities. For most of the wedges, maximum safe cut slope angles greater than $45^{\circ}$ were obtained. Maximum safe slope angles greater than 80" were obtained fur most of the discontinuities in the toppling case. The block theory analysis resulted in the identification of potential key blocks (type II) in the SL4, SL5, SL6 and SL8 sections. The chance of sliding taking place through a type ll block under a combined gravitational and external loading is quite high in the investigated area. The results support in-field observations of a potentially unstable slope that could become hazardous under external forces. The results obtained through limit equilibrium slope stability analyses show how a stable slope can become an unstable slope as the water pressure acting on joints increases and how a stable slope under Barton's shear strength criterion can fail as the worst case scenario of using Mohr-Coulomb criterion.

• Clinical and Experimental Pediatrics
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• v.51 no.11
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• pp.1158-1164
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• 2008

Purpose : To investigate the discriminative ability of pediatric index of mortality 2 (PIM2) and pediatric risk of mortality III (PRISM III) in predicting mortality in children admitted into the intensive care unit (ICU). Methods : We retrospectively analyzed variables of PIM2 and PRISM III based on medical records with children cared for in a single hospital ICU from January 2003 to December 2007. Exclusions were children who died within 2 h of admission into ICU or hopeless discharge. We used Students t test and ANOVA for general characteristics and for correlation between survivors and non-survivors for variables of PIM2 and PRISM III. In addition, we performed multiple logistic regression analysis for Hosmer-Lemeshow goodness-of-fit, receiver operating characteristic curve (ROC) for discrimination, and calculated standardized mortality ratio (SMR) for estimation of prediction. Results : We collected 193 medical records but analyzed 190 events because three children died within 2 h of ICU admission. The variables of PIM2 correlated with survival, except for the presence of post-procedure and low risk. In PRISM III, there was a significant correlation for cardiovascular/neurologic signs, arterial blood gas analysis but not for biochemical and hematologic data. Discriminatory performance by ROC showed an area under the curve 0.858 (95% confidence interval; 0.779-0.938) for PIM2, 0.798 (95% CI; 0.686-0.891) for PRISM III, respectively. Further, SMR was calculated approximately as 1 for the 2 systems, and multiple logistic regression analysis showed ${\chi}^2(13)=14.986$, P=0.308 for PIM2, ${\chi}^2(13)=12.899$, P=0.456 for PRISM III in Hosmer-Lemeshow goodness-of-fit. However, PIM2 was significant for PRISM III in the likelihood ratio test (${\chi}^2(4)=55.3$, P<0.01). Conclusion : We identified two acceptable scoring systems (PRISM III, PIM2) for the prediction of mortality in children admitted into the ICU. PIM2 was more accurate and had a better fit than PRISM III on the model tested.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.246-253
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• 2011

This experiment was carried out to examine the residual amount variation of carbendazim and chlorpyrifos in fruit parts of red pepper according to the open field and/or the rain shelter house. It was shown higher residual amounts of agrochemicals in the field than the house condition at two hours (0 day) after chemical application, but it was shown higher residual amounts in the house at 5 days and 10 days. Although the residual amount in the field was higher than in the house at the beginning, the chemicals fast degraded in field condition. Carbendazim and chlorpyrifos remained as time passed in order of receptacle, but the residual amounts of two chemicals in leaf at $5^{th}$ day. Carbendazim and chlorpyrifos were lessened until 10 days after chemical application, and reduction rate were 19.1% and 66.4% in flesh, 45.2% and 62.3% in receptacle, and 41.6% and 72.0% in the stalk, respectively. The reduction rate at 15 days showed 31.1%, 75.3% in flesh, 43.5%, 81.7% in receptacle, and 47.7%, 82.8% in stalk, respectively. Therefore the reduction rate of carbendazim showed receptacle > stalk > flesh, and that of chlorpyrifos showed stalk > receptacle > flesh in order. The calculated half-life of carbendazim showed 29.6 days in flesh, 13.6 days in receptacle, and 16.0 days in stalk, but that of chlorpyrifos showed 8.3 days in flesh, 8.3 days in receptacle, and 6.3 days in stalk. In conclusion, the half-life of carbendazim was longer than that of chlorpyrifos in even part, and especially was longest in flesh part of fruit.

• Korean Journal of Environmental Biology
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• v.28 no.3
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• pp.133-142
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• 2010

This study was performed to investigate the community structure of the macrobenthic invertebrates around Ui Island, Sinan-gun, Jeollanam-do, Korea. Benthic invertebrates were collected during May and September 2008 at seven stations. A total of 63 macrobenthic species was collected. The overall average macrobenthos density and biomass were 268 inds. $m^{-2}$ and 11.54 gWWt $m^{-2}$, respectively. Based on abundance data, there were 5 dominant species accounting for approximately 72.00% of total individuals. The polychaetes Notomastus latericeus, Scolelepis sagittaria, Amaeana occidentalis, Glycera chirori and the amphipoda Mandibulophoxus mai were found in high densities. The conventional multi-variate statistics (cluster analysis and non-metric multidimensional scaling) applied to assess spatial variation in macrobenthic assemblages. As a result, three communities could be distinguished: a sand dominated, a mixed sediment and a third community in the mud dominated station. The community in the sand dominated station was characterized by high abundance of the polychaetes S. sagittaria and the amphipods M. mai, Monoculodes sp. and Grandifoxus malipoensis. However, the most common species within the mixed sediment station were the polychaetes N. latericeus, A. occidentalis and amphipoda Urothoe sp.. Finally the mud dominated station was numerically dominated by the polychaetes Scolelepis sp., Heteromastus filiformis and Sigambra tentaculata. In conclusion, the sediment composition may be important factors controlling of the macrobenthic community structure in the study area.

• Journal of Veterinary Clinics
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• v.26 no.2
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• pp.117-122
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• 2009

The aim of this study is to investigate the effects of surfactin in combination with Bacillus subtilis BC1212 isolated from Korean soybean paste, on feed utilization and growth performance during 4 weeks in weaning piglets. Eighteen weaning piglets(Landrace$\times$Yorkshire$\times$Duroc; weighing $7.68{\pm}0.97\;kg$) were divided into control(n=9) and experimental groups(n=9). The treatments included a control group consisting of the basal diet with no additives(control) and an experimental group consisting of the basal diet supplemented with 1 g of surfactin C and $1.0{\times}10^9CFU$ of Bacillus subtilis BC1212/kg feed. Piglets fed Bacillus subtilis BC1212 increased in average daily weight gain and feed efficiency. In comparison with the control group, the fecal Bacillus subtilis were significantly increased and the fecal coliform bacteria were markedly reduced in the experimental group. In addition, Bacillus subtilis BC1212 had excellent acid and bile tolerance. The treatment of surfactin($50{\mu}g\;ml^{-1}$) in lipopolysaccharide(LPS)-stimulated swine peripheral blood mononuclear cells(PBMCs) for 6 h showed a significant inhibitory effect on INF-$\gamma$, TNF-$\alpha$ and NO secretion(p<0.05) in comparison with LPS treatment alone but not on IL-10 secretion, with levels of secreted IL-10 similar to those secreted by PBMCs stimulated with LPS alone. Supplementation with surfactin in combination with Bacillus subtilis BC1212 in diets improved the ecosystem of gastrointestinal tract by increasing probiotic population and enhanced the systemic immune response in weaned piglets.

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.331-338
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• 2007

One-node stem pieces ca. 1 cm in length containing a axillary bud and a fully expanded leaf were obtained from it in vitro plants of potato (Solanum tuberosum L.). Leaves were removed and the nodes were cultured on the MS medium to investigate the effects of temperature, day length, sucrose, and CCC in microtuber formation and development. The fresh weight of microtubers after 80 days increased significantly at 8% sucrose and $20^{\circ}C$ compared with $28^{\circ}C$. The tuberization and development were reduced at $28^{\circ}C$ except short-day treatment of 8 hours at 8% sucrose. The fresh weight and diameter were increased on the culture medium added CCC 500 mg/L. The potato tuberization was promoted under short daylength, and it showed great effect by treatment with the CCC. Though the tuberization was promoted at low temperature of $20^{\circ}C$ in a histologic change of an axillary bud part cell of a potato, the cells were able to observe the swelling growth. Swelling growth of tissue was stimulated in the darkness and was more remarkable by addition of CCC. In particular, in the visual ratio of cell division for each position in the tissue, the cortex part showed larger ratio of cell expansion than that of the pith part. The effect of CCC was identified at 8% sucrose in the darkness. The effect of CCC was not showed in sucrose 3% under long daylength of 16 hours. As a result, the fact of a substance with AGPase important for starch composition was certified by the result with the inclose of AGPase activity on high concentration of sucrose, CCC, and dark treatment by which tuber formation and development are promoted.

• Journal of the Korean Wood Science and Technology
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• v.11 no.3
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• pp.58-69
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• 1983

This research is performed to compare lignin compounds isolated in Pinus koraiensis Sieb et Zucco, with those compounds which have been already isolated. and to elucidate IR spectra of functional groups and aromatic nucleus for chemical structure of lignin. In vanillin and dehydrodivanillin having carbonyl group ill ${\alpha}$-position. characteristic absorption band of carbonyl group was confirmed in 1665 $cm^{-1}$. Absorption band of vanillic and syringic acid with carboxyl group were indicated in 1675 and 1690 $cm^{-1}$ respectively. Syringic acid showed low wave number because this was affected by methoxyl group. Characteristic absorption bands of guaiacyl, syringly and guaiacyl-syringyl co-polymer nucleus were clearly disclosed in diarylpropane compounds. It were reconfirmed that absorption band of guaiacyl showed longer wave number than of syringly nucleus.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.452-457
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• 2007

In this study, Lilium sp. were separated into bulbs, leaves, and flowers. Then, total polyphenol contents, electron donating ability (EDA), superoxide dismutase (SOD)-like activity, hydroxyl radical scavenging activity, and antimicrobial activity were measured from the extracts of each of the three aforementioned parts. The examination of physiologically active substances in the three parts revealed that Lilium davidii leaves had high total polyphenol contents, SOD-like activity, hydroxyl radical scavenging activity, and EDA, while the flowers of L. lancifolium showed high SOD-like activity, hydroxyl radical scavenging activity, and EDA, as well as a high level of total polyphenols in the bulb. Measurements of the antimicrobial activities of the extracts against Gram positive bacteria revealed that the leaves and flowers of L. davidii and L. lancifolium caused Bacillus subtilis and Salmonella enteritidis to form clear zones greater than 10 mm. Furthermore, the flowers of L. lancifolium showed particularly high antimicrobial activity against B. subtilis, and the flowers of L. davidii had high activity against S. enteritidis. For the Gram negative bacteria, the leaves and flowers of L. davidii and L. lancifolium caused Listeria monocytogenes and Escherichia coli to form clear zones greater than 10 mm, and finally, the flowers of L. davidii and L. lancifolium showed high antibacterial activity, with inhibition exceeding 12 mm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.216-226
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• 2019

This study was to investigate the effect of the horse grazing intensity on the vegetation of the S. quelpaertensis, and the physiological changes of the horse. This experiment was conducted at the community of the S. quelpaertensis (altitude of 550m) with the grazing intensity of 1.0AU(500kg), 1.5AU(750kg) and 2.0AU(1,000kg) at 30m × 30m for one week, and then the growth characteristics of the S. quelpaertensis, the changes of the vegetation, the damages on trees and the changes in the physiological characteristics of the horses was investigated and compared before and after the horse grazing. As the results, the 2.0AU grazing land showed that the culm number had the inverse correlation (r=-0.902, P<0.01) to the other lands and the species diversity index of lower-level vegetation showed the correlation (r=0.773, P<0.01) as increasing the grazing intensity.