• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 추계학술대회 논문집 전력기술부문
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• pp.14-16
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• 2003

For four decades now KEPCO has experienced an incredible growth rate of over 10% per year in each year. This growth led to the fast development of new facilities, especially in the transmission systems field, and we also managed to gain an enormous amount of experience and technology in the construction and design. With this experience and technology we curbed the electricity demand. Experts now predict that we will enter a new phase in which our growth rate will stagnate and eventually level off. This isn't what KEPCO or any company wants. We are looking for continued growth. In the last decade KEPCO began selling its technology in the generation sector of the electricity business to overseas markets. Sales in the generation sector have been a great success. This decade marks another milestone for KEPCO as overseas sales in the transmission sector began. Our first project, "Myanmar Power System Development Study" and its subsequent successful completion gave us the ammunition necessary to expand in this line of business. This success has also given birth to this paper. KEPCO is still the People's company, but with a new vision. It now hopes to not only sustain a high growth level but to also increase the growth of domestic companies by sharing with them a piece of the proverbial pie, as it ventures forth to be a Global player on the international stage.

• The Korean Journal of Physiology
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• 제24권2호
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• pp.281-291
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• 1990

The $Ca^{2+}-substitutional$ roles of strontium for the contractile processes were investigated in the rabbit renal artery. The contractions induced by either norepinephrine or high $K^+$ in the condition which intra- and extracellular $Ca^{2+}$ were replaced by $Sr^{2+}$, i.e. $Sr^{2+}-mediated$ contractions, were dose-dependent. And then the maximal amplitude of contraction, as compared with $Ca^{2+}-mediated$ contraction, was about 50% in norepinephrine and about 70% in high $K^+$. The $Sr^{2+}-mediated$ contractions were independent in the contraction by norepinephrine $(10^{-5}M)$ but dependent in those by high $K^+(100\;mM)$ on the extracellular $Sr^{2+}$ concentration. Also $Sr^{2+}-mediated$ contractions induced by norepinephrine were observed in the $Sr^{2+}-free$ Tyrode's solution. The $Sr^{2+}-mediated$ contractions induced by either norepinephrine or high $K^+$ were suppressed by verapamil, a $Ca^{2+}-channel$ blocker. By extracellular addition of $Sr^{2+}$, the $Ca^{2+}-mediated$ contractions induced by norepinephrine $(10^{-5}M)$ or 40 mM $K^+$ were inhibited but those by high $K^+(100\;mM)$ were increased. And the $Sr^{2+}-mediated$ contractions were increased by extracellular addition of $Ca^{2+}$ but did not reach the level of $Ca^{2+}-mediated$ contraction. Therfore it is suggested that in the vascular smooth muscle of rabbit renal artery $Sr^{2+}$ could enter the smooth muscle cells easily through the potential-operated calcium channel (POC) but not easily through the receptor-operated calcium channel (ROG), and $Sr^{2+}$ might be stored in the intracellular $Ca^{2+}-binding$ site and released by NE and induced the contraction by a way of activating directly the contractile apparatus.

• The Korean Journal of Physiology and Pharmacology
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• 제20권5호
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• pp.539-545
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• 2016

Nafamostat mesilate (NM), a synthetic serine protease inhibitor, has anticoagulant and anti-inflammatory properties. The intracellular mediator and external anti-inflammatory external signal in the vascular wall have been reported to protect endothelial cells, in part due to nitric oxide (NO) production. This study was designed to examine whether NM exhibit endothelium dependent vascular relaxation through Akt/endothelial nitric oxide synthase (eNOS) activation and generation of NO. NM enhanced Akt/eNOS phosphorylation and NO production in a dose- and time-dependent manner in human umbilical vein endothelial cells (HUVECs) and aorta tissues obtained from rats treated with various concentrations of NM. NM concomitantly decreased arginase activity, which could increase the available arginine substrate for NO production. Moreover, we investigated whether NM increased NO bioavailability and decreased aortic relaxation response to an eNOS inhibitor in the aorta. These results suggest that NM increases NO generation via the Akt/eNOS signaling pathway, leading to endothelium-dependent vascular relaxation. Therefore, the vasorelaxing action of NM may contribute to the regulation of cardiovascular function.

• The Korean Journal of Physiology and Pharmacology
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• 제16권3호
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• pp.199-204
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• 2012

We evaluated the role of Tat-mediated p66shc transduction on the activation of endothelial nitric oxide synthase in cultured mouse endothelial cells. To construct the Tat-p66shc fusion protein, human full length p66shc cDNA was fused with the Tat-protein transduction domain. Transduction of TAT-p66shc showed a concentration- and time-dependent manner in endothelial cells. Tat-mediated p66shc transduction showed increased hydrogen peroxide and superoxide production, compared with Tat-p66shc (S/A), serine 36 residue mutant of p66shc. Tat-mediated p66shc transduction decreased endothelial nitric oxide synthase phosphorylation in endothelial cells. Furthermore, Tat-mediated p66shc transduction augmented TNF-${\alpha}$-induced p38 MAPK phosphorylation in endothelial cells. These results suggest that Tat-mediated p66shc transduction efficiently inhibited endothelial nitric oxide synthase phosphorylation in endothelial cells.

• Journal of Applied Biological Chemistry
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• 제59권2호
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• pp.155-158
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• 2016

산복사나무(Prunus davidiana) 열매를 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, 물 분획으로 나누었다. EtOAc 분획에 대하여 $SiO_2$과 ODS column chromatography를 반복 실시하여 3종의 화합물을 분리 및 정제하였다. NMR, MS, 및 IR data 등을 해석하여, ursolic acid (1), corosolic acid (2), 그리고 ${\alpha}-amyrin$ (3)로 구조동정 하였다.

• 농촌계획
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• 제15권1호
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• pp.89-99
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• 2009

I wish to establish Kwang-shi Han-woo agriculture theme park furtherance plan that take advantage of unique area resource of homebred cattle meat sale restaurant of the Chungchong-namdo Yesan County Kwang-Shi Ran-woo village. Improve regional income that develops Kwang-shi Ran-woo area that is made up a rest and experience space that escapes in sightseeing of Ran-woo meat putting first that is food, and contribute in economy activation by promotes City-Farm exchange, and is behind present, and is expected that contributes in farm village area activation that is stagnated Ran-woo specialization area of whole country scale. Kwang-shi Han-woo agriculture-farm village theme park plan is thought that active participation of thorough environmental protection and local resident is necessary condition as plan that take advantage of peculiar latency resource of area. In plan process, reflected various opinion of local resident representatives to space but there is a little insufficient page, and sees that also thorough environment analysis should be preceded. Hereafter, see that correction' repletion need a little through thorough grasping of latency resource that put together thorough natural environment investigation. in Yesan County with analysis at final design step. But, see that plan of theme park that take advantage of characteristic resource in farm village, especially situation of Ran-woo farmhouses that is suffering now difficulty as plan that do appropriateness of city in area activation and economical income guarantee side of farmhouse inhabitants.

• Natural Product Sciences
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• 제13권2호
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• pp.123-127
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• 2007

We investigated effect of small black soybean fraction (SBSF) T cell-mediated responses for tumor surveillance and proliferation in leukemia cells in vitro. Each SBSF butanol fraction (SBSFBu) and SBSF chloroform fraction (SBSFCh) was administered p.o. once a day far 21 days in BALB/c mice and then levels of serum cytokines and subpopulation of lymphocytes were measured. Moreover, SBSF fraction was treated into the cultured various cell lines for proliferation in leukemia cell lines, NO production by RAW264.7 cells, and expression of p53 gene in U937 leukemia cells. These results showed that SBSFBu increased levels of serum IL-4but not IL-2 and IFN-${\gamma}$, and increased expression of CD4$^+$ T cells and CD8$^+$ T cells in splenocytes in vivo, while SBSFCh increased levels of serum IL-2 and IFN-${\gamma}$ but decreased IL-4, and increased CD8$^+$ T cells but not CD4$^+$ T cells. Moreover, both of SBSFBu and SBSFCh inhibited proliferation of HL60, U937, and L1210 leukemia cell lines in a dose-dependent manner, up-regulated NO production by RAW264.7 cells in a dose-dependent manner, and enhanced expression of p53 gene in U937 leukemia cells. Our findings indicate that SBSFBu and SBSFCh may enhance T cell-dependent immune responses, and that both of SBSFBu and SBSFCh may inhibit proliferation of leukemia cells by up-regulation of NO production and expression of p53 gene.

• 동의생리병리학회지
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• 제20권1호
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• pp.273-278
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• 2006

This survey was accomplished in order to find out the necessity of creation of Korean standard differentiation of the symptoms and signs for the stroke(KSDS) in clinical practice. The survey questions were developed by the consensus from the professors who major in internal traditional Korean medicine(TKM). May 11th to July 31th 2005, the questionnaire was given to 800 TKM doctors in the whole country of Korea listed on the National Korean Medical Hospital Associations address book dy postal mail. Total of 358(44.7%) questionnaires were retrieved out of the 800 subjects. The 311 (86.9%) of 358 TKM doctors given an answer to the survey responded to need development of KSDS, and 176(56.6%) of 311 TKM doctors who answered to need development of KSDS responded to have to consider consensus of clinical experts on the stroke for creation of KSDS. These results support a role for new KSDS might be usefully applied in diagnosis and treatment of the stroke.

• 동의생리병리학회지
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• 제21권3호
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• pp.709-713
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• 2007

Basement membranes (BMs) are extracellular matrices associated with epithelia, endothelia, muscle, fat and peripheral nerve. They are involved in cell survival, migration, differentiation. BMs functions also include tissue formation and provide mechanical stability as a selective barriers. Laminins are heterotrimeric glycoproteins found in BMs and have a crucial role in cell adhesion and signalling. Madin-Darby canine kidney (MDCK) cells are the best established mammalian model for studying epithelial cell biology The cells form an epithelial monolayer, with tight junctions separating an apical surface from a basolateral membrane facing the filter support and neighboring cells. In this study, using MDCK cells, the synthesis of the BM protein such as laminin with or without methanol extract of Chrysanthemum morifolium (CM) stimulation was analyzed by immunoblotting and CM showed significant increased cell density and enhanced synthesis of laminin.

• 한국수산과학회지
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• 제32권5호
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• pp.664-668
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• 1999

생분해성이며 가식성 alginate 필름을 가공하기 위하여 추출용 액의 $Na_2CO_3$ 농도와 추출시간을 달리하여 alginate를 추출하고, 동 alginate로 제조한 alginate 필름의 수증기 투과율과 장력성의 변화를 측정하였다. 추출용액중의 $Na_2CO_3$ 농도의 증가 및 추출시간의 연장과 더불어 alginate의 점도와 중합도는 감소하였다. 저점도 alginate로 만든 alginate 필름은 높은 수증기투과율을 보였다. 가소제로서 사용된 sorbitol과 polyethylene glycol 첨가는 필름의 수증기투과율과 인장강도를 현저히 낮추는 효과를 보였다. Alginate의 추출시간은 alginate필름의 수중기투과율과 신장성에 미치는 영향이 적었으나 alginate 중합도는 인장강도에 직접적으로 영향을 미쳤다.