Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Triterpenoids from the fruits of Prunus davidiana

산복사나무(Prunus davidiana) 열매로부터 Triterpenoid의 분리 및 동정

  • Lee, Min-Jee (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Kim, Ji-Hye (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Cha, Byeong-Ju (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Lee, Youn-Hyung (Department of Horticultural Biotechnology, Kyung Hee University)
  • Received : 2015.12.29
  • Accepted : 2016.04.06
  • Published : 2016.06.30
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Abstract

The fruits of Prunus davidiana were extracted with 80 % aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-butyl alcohol, and water fractions. From the EtOAc fraction, three triterpenoids were isolated through the repeated silica gel ($SiO_2$) and octadecyl $SiO_2$ column chromatographies. Based on physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the compounds were identified to be ursolic acid (1), corosolic acid (2), and ${\alpha}-amyrin$ (3).

산복사나무(Prunus davidiana) 열매를 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, 물 분획으로 나누었다. EtOAc 분획에 대하여 $SiO_2$과 ODS column chromatography를 반복 실시하여 3종의 화합물을 분리 및 정제하였다. NMR, MS, 및 IR data 등을 해석하여, ursolic acid (1), corosolic acid (2), 그리고 ${\alpha}-amyrin$ (3)로 구조동정 하였다.

Keywords

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