• 한국식품저장유통학회지
• /
• 제12권6호
• /
• pp.637-642
• /
• 2005

일상생활에서 식용 및 약용으로 유통되는 12종의 버섯류의 추출물을 제조하여 7종의 식중독균에 대한 항균 활성과 함께 인간유래의 간암 및 결장암 세포주의 증식에 미치는 영향을 조사하였다. 먼저 버섯 메탄올추출물은 7종의 식중독균에 대해 항균활성을 보였으나, 버섯 열수추출물은 이들 균에 대해 생육 억제활성이 없거나 약한 억제 활성을 보였다. 만가닥버섯과 느타리버섯 메탄올추출물은 7종의 식중독균에 대해 항균 활성을 보였고, 양송이버섯, 새송이버섯, 애느타리버섯도 6종의 식중독균에 대해 항균 활성을 보였다. 또한 버섯 메탄올추출물은 인간유래 결장암세포인 HT-29보다는 간암세포인 HepG2에 대해 더 높은 성장 저해능을 보였다. HepG2에 대해 번데기동충하초 메탄올추출물이 $67\%$의 가장 높은 성장 저해율을 나타내었고, HT-29에 대해서는 향버섯 메탄올추출물이 $81.1\%$의 가장 높은 저해율을 보였다. 그리고 번데기동충하초, 아카리쿠스, 영지버섯, 향버섯 열수추출물에서 HepG2 및 HT-29에 대해 $50\%$ 정도의 억제 활성을 볼 수 있었고, 다른 버섯 열수추출물은 억제활성을 볼 수 없었다. 특히 번데기동충하초, 아카리쿠스, 만가닥버섯, 영지버섯, 향버섯 메탄올 및 열수추출물은 이들 암세포주에 대해 높은 증식 억제 활성을 보였다.

• Journal of Microbiology and Biotechnology
• /
• 제31권4호
• /
• pp.610-620
• /
• 2021

There has been increasing interest in the head and neck squamous cell carcinoma (HNSCC) that is caused by high-risk human papillomavirus (HR-HPV) and has posed a significant challenge to Otolaryngologists. A rapid, sensitive, and reliable method is required for the detection of HR-HPV in clinical specimens to prevent and treat HPV-induced diseases. In this study, a multiple cross-linking spiral amplification (MCLSA) assay was developed for the visual detection of HPV-16. In the MCLSA assay, samples were incubated under optimized conditions at 62℃ for 45 min, and after mixing with the SYBR Green I (SGI) dye, the positive amplicons showed bright green fluorescence while the negative amplicons exhibited no obvious change. The specificity test revealed that the developed MCLSA technique had high specificity and could effectively distinguish all five HPV-16 strains from other pathogenic microorganisms. In terms of analytical sensitivity, the limit of detection (LoD) of MCLSA assay was approximately 5.4 × 101 copies/tube, which was 10-fold more sensitive than loop-mediated isothermal amplification (LAMP) and RT-PCR. The detection results of laryngeal cancer specimens collected from 46 patients with suspected HPV infection in the Liaoning region demonstrated that the positive detection rates of MCLSA and hybridized capture 2 kit were 32.61% (15/46). The true positive rate of the MCLSA assay was higher than that of RT-PCR (100% vs. 93.33%) and LAMP (100% vs. 86.67%). Therefore, the MCLSA assay developed in the present study could be a potentially useful tool for the point-of-care (PoC) diagnosis of HR-HPV, especially in resource-limited countries.

• 대한화장품학회지
• /
• 제33권1호
• /
• pp.47-52
• /
• 2007

[ $C_{8-12}$ ]의 medium-chain free fatty acid 및 1-monoglyceride의 다양한 미생물에 대한 항균력은 잘 알려져 있다. Lipid의 항균력에 대해 발표된 연구는 추로 사람이나 가축에 감염을 일으키는 병원성 세균 및 바이러스에 대한 항균력에 초점을 맞춰 왔으며, 실제로 화장품에 문제가 되는 미생물에 대한 체계적인 항균력 조사는 전무한 실정이다. 우선, 본 연구에서는 $C_{12}$ (glyceryl laurate)에 비해 $C_8$ (glyceryl caprylate) 사슬 길이를 가지는 1-monoglyceride의 화장품 제형 내 방부력을 조사해 보았다. 그 결과, $C_8}$ 및 $C_{12}$의 chain length를 가지는 1-monoglyceride 모두 세균에 대한 방부력은 매우 우수한 반면, 진균에 대한 방부력이 취약함을 확인하였다. 또한, $C_{12}$에 비해 $C_8$의 사슬 길이를 가지는 glyceryl caprylate는 항균력이 약간 우수하였다. 이 후 화장품 방부력 시험에 사용되는 5종의 미생물 각각에 대한 glyceryl caprylate의 항균력을 알아보기 위한 시험 결과, 그람양성균인 S. aureus와 이스트인 C. albicans에 대한 항균력이 매우 우수하게 나타났으며, 그람음성균인 E coli, P. aeruginosa 순으로 우수한 항균력을 나타내었다. 하지만, 곰팡이인 A. niger에 대해서는 다른 균에 비해 매우 취약한 항균력을 나타내었다. 따라서 본 연구에서는 glyceryl caprylate의 화장품 방부제로서의 적용을 위해 A. niger와 같은 곰팡이에 대한 항균력을 높고자 하였으며, 그 결과 glyceryl caprylate의 A. niger에 대한 항균력이 산성 조건에서 현저히 증가하는 것으로 나타났다. 또한, 7종의 다른 antimicrobial agent와의 병용을 통한 최적의 조합을 찾고자 실험한 결과, methylparaben과 조합했을 때 진균에 대한 항균력이 가장 좋아지는 것을 확인하였다.

• 한국식품영양학회지
• /
• 제22권3호
• /
• pp.396-401
• /
• 2009

본 실험은 경기 북부 일부 지역의 계란에서 미생물 오염도를 측정하여 계란의 위생 상태를 파악하고자 하였다. 조사 자료로서는 대형 마트에 유통 중인 계란 중에서 서로 브랜드가 다른 계란을 대상으로 하였다. 계란의 난각에 존재하는 일반 세균수를 측정하였다. 그 결과 $3.4{\times}10^4cfu/m{\ell}$로 상당히 높은 일반 세균수가 검출되었다. 다음으로는 식중독 지수의 중요한 지표가 되는 대장균 및 계란으로 인한 식중독에서 주요 원인 균으로 작용하는 살모넬라균의 오염 여부를 파악한 결과, 한 브랜드의 계란에서 대장균이 검출되었고, 살모넬라균은 검출되지 않았다. 또한 각 브랜드에 계란에서 검출된 세균을 분리 동정 한 결과, Klebsiella pneumoniae, Pseudomonas mendocina, Alcaligenes xylosoxidans, Alcaligenes faecalis, Enterobacter cloacae 등이 분리되었다. 이 세균들 중 일부는 사람에게 병원성을 나타내거나, 면역력이 약한 노약자나 어린이에게 기회감염 또는 병원 내 감염을 일으키기도 하는 것으로 알려졌다. 계란의 난각에서 분리된 대장균의 병원성 여부를 판정하기 위하여, 대장균의 DNA를 분리하여 PCR을 수행한 결과, 병원성 대장균들에서 특이적으로 나타나는 유전자형을 가지고 있지 않는 것으로 밝혀졌다. 위의 결과들을 종합하여 보면, 시중에 유통중인 계란의 난각에서 비록 살모넬라균과 병원성 대장균은 검출되지 않았지만, 사람에게 감염을 일으킬 수 있는 여러 병원성 세균들이 검출됨으로써 계란의 유통 및 판매에 더욱 철저한 위생관리가 필요할 것으로 사료된다.

• 셀메드
• /
• 제4권1호
• /
• pp.5.1-5.8
• /
• 2014

Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.94-94
• /
• 2018

The gold (LC-AuNPs) and silver (LC-AgNPs) nanoparticles were rapidly synthesized by fruit extract of Lycium chinense within 1.15 and 25 min respectively in an eco-friendly way. The synthesized nanoparticles confirmed by relevant surface plasmon resonance peaks for gold and silver nanoparticles at 536 and 480 nm, respectively. FE-TEM results revealed that LC-AuNPs were 20-50 nm and LC-AgNPs were 50-100 nm. The maximum distribution of gold, silver elements and the crystallographic nature of synthesized were confirmed using EDX, elemental mapping and XRD. LC-AgNPs showed inhibitory activity against pathogenic microorganisms such as E. coli and S. aureus, whereas LC-AuNPs did not show inhibitory activity. The LC-AgNps nanoparticles exhibited significant cytotoxicity to human breast cancer MCF7 cell line and less cytotoxicity to non-diseased RAW264.7 (murine macrophage) cells whereas LC-AuNps showed minimal toxicity to both cell lines. In-depth research on this rapid, facile and greenery nanoparticles may play a potential role in biomedical applications.

• Journal of Microbiology and Biotechnology
• /
• 제30권9호
• /
• pp.1305-1309
• /
• 2020

Insects possess biological defense systems that can effectively combat the invasion of external microorganisms and viruses, thereby supporting their survival in diverse environments. Antimicrobial peptides (AMPs) represent a fast-acting weapon against invading pathogens, including various bacterial or fungal strains. A 37-residue antimicrobial peptide, papiliocin, derived from the swallowtail butterfly Papilio xuthus larvae, showed significant antimicrobial activities against several human pathogenic bacterial and fungal strains. Jelleines, isolated as novel antibacterial peptides from the Royal Jelly (RJ) of bees, exhibit broad-spectrum protection against microbial infections. In this study, we developed a novel antimicrobial peptide, PAJE (RWKIFKKPFKISIHL-NH₂), which is a hybrid peptide prepared by combining 1-7 amino acid residues (RWKIFKK-NH₂) of papiliocin and 1-8 amino acid residues (PFKISIHL-NH₂) of Jelleine-1 to alter length, charge distribution, net charge, volume, amphipaticity, and improve bacterial membrane interactions. This novel peptide exhibited increased hydrophobicity and net positive charge for binding effectively to the negatively charged membrane. PAJE demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria, with very low toxicity to eukaryotic cells and an inexpensive process of synthesis. Collectively, these findings suggest that this novel peptide possesses great potential as an antimicrobial agent.

• Journal of Microbiology and Biotechnology
• /
• 제32권11호
• /
• pp.1416-1426
• /
• 2022

The need to discover new types of antimicrobial agents has grown since the emergence of antibiotic-resistant pathogens that threaten human health. The world's oceans, comprising complex niches of biodiversity, are a promising environment from which to extract new antibiotics-like compounds. In this study, we newly isolated Pseudomonas sp. NIBR-H-19 from the gut of the sea roach Ligia exotica and present both phenotypes and genomic information consisting of 6,184,379 bp in a single chromosome possessing a total of 5,644 protein-coding genes. Genomic analysis of the isolated species revealed that numerous genes involved in antimicrobial secondary metabolites are predicted throughout the whole genome. Moreover, our analysis showed that among twenty-five pathogenic bacteria, the growth of three pathogens, including Staphylococcus aureus, Streptococcus hominis and Rhodococcus equi, was significantly inhibited by the culture of Pseudomonas sp. NIBR-H-19. The characterization of marine microorganisms with biochemical assays and genomics tools will help uncover the biosynthesis and action mechanism of antimicrobial metabolites for development as antagonistic probiotics against fish pathogens in an aquatic culture system.

• 한국산업보건학회지
• /
• 제10권1호
• /
• pp.115-125
• /
• 2000

Three major hospitals with over 500 beds located in and near Seoul were surveyed for airbone microorganisms from February 1, 1998 to February 18, 1998. The purpose of the study was to identify and quantify microbiological organisms circulating in the air of three different areas in the hospitals. For the study, a RCS air sampler was utilized equipped with two different collection media, the agar strip GK-A for bacteria and the agar strip HS for fungi. The areas investigated were the intensive care unit (ICU) in the Department of internal medicine, the Newborns room in the Department of Obstetrics, and the microbiology laboratory. The results were as follows; 1. The average numbers of general microbiological particles collected on the agar strip GK-A media were $205CFU/m^3$, $232CFU/m^3$, and $128CFU/m^3$ in each hospitals. The highest concentration of $387CFU/m^3$ was found in the ICU of A hospital at 15:00 during the day. Further analysis of the collected bioaerosols by gram staining, revealed that there were gram positive cocci (89.5%), gram positive bacilli (7.2%), gram negative bacilli (2.8%), and fungi (0.5%), in descending order of frequency. 2. Ten different genes were identified from the agar strip GK-A. The most frequently identified organisms were: the Coagulase negative staphylococcus (55.0%), Micrococcus (21.4%), Enterococcus species(10.4%), and Bacillus species (7.2%). A series of antibiotics susceptibility test were conducted against the aforementioned four(4) organisms. Ninety percent of coagulase negative stapylococcus were sensitive to Penicillins. Pathogenic microbes isolated include: Staphylococcus aureus, Acinetobacter species, Klebsiella pneumonia, Klebsiella oxytoca, and Pseudomonas aeroginosa. 3 Although 56.8% of the microorganisms grown on the strip HS media for fungi could not be identified, some of them were successfully identified. The most frequently found fungi were Aspergillus (35.3%), Yeast or Molds (6.2%), and Penicillium (0.7%). Based on the results obtained from the study, it was concluded that some areas in the hospitals had abnormally high bioaerosol concentrations which could be attributed to human activity. Therefore, it is recommended that periodic assessments of indoor bioaerosols aiming to identify the possible sources should be conducted in order to maintain clean indoor environment in the hospitals.