• International Journal of Knowledge Content Development & Technology
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• 제7권4호
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• pp.27-50
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• 2017

The impact of social networking is varied from good to bad. Online activities have also been categorized into pros and cons of social networking, either as reported as hiding Internet activities among teenagers or killing loneliness among elderly. In terms of relationships, there have been argument over its closeness and quality of an online relationship in Internet settings. Looking at the contradiction in an innovative interaction between classic community communication and social media, there is an unknown scent of the future struggling and challenging both human communication and relationships in the presence of digital culture. This research uses Diffusion of Innovation to study the wide and continuous spread of digital culture in human communication; and, Media Dependency in learning and structuring the cognitive, affective and behavioral effects of social media on each person uses the media in different ways. This research will be using online survey to gain opinions from a social network site as an update of views and reflection of self-awareness to all levels of people. Social media like Facebook (FB) is perceived as a good tool of communication that it is able to bring closeness among the family members. The results show that social media like FB brings positive impact towards family members; it would help to build a better and harmonic society; and, relationships among family members and communication shall be improved and enhanced to the level of a united society.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1139-1141
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• 2009

Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significant HNE inhibitory activity with an $IC_{50}$ value of $17.8{\mu}M$.

• Journal of Microbiology and Biotechnology
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• 제12권6호
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• pp.882-889
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• 2002

Hantaan vims production, using human immortalized retina cell (PER. C6), was investigated to develop an inactivated virus vaccine. To infect Hantaan virus into PER. C6, two infection methods (medium-to-cell and cell-to-cell) were tried, and IFA results showed that the cell-to-cell infection method was very useful for producing Hantaan virus-infected PER, C6. Hantaan virus production was significantly affected by the growth rate of PER. C6 and the content of FBS in medium. Higher specific growth rate of infected PER. C6 and lower FBS content induced higher production of Hantaan virus. The inactivated human cell-culture vaccines with various EIA titers were prepared, their antibody responses were compared with those of inactivated suckling mouse brain vaccines ($Hantavax^처리불가$). and the result showed their immunogenicities were slightly higher than those of inactivated suckling mouse vaccines. Therefore, this study shows the possibility of the development of Hantaan virus vaccine from a human cell culture.

• 한국디지털건축인테리어학회논문집
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• 제9권3호
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• pp.37-45
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• 2009

New city development policy caused many effects such as destruction of nature and area native culture extinction through fast development within short period. The architectural context is remains by post-modern most difficult problem had left to architects. We need to find the design method that communicate and contacts character of place, cultural context through the actual project. In this study, we propose the method of aspect of compose space and design elements to succeed the cultural place and context, desirable development through the design proposal for Dochon welfare center. In Dochon area, there is no original character of the place because of indiscreet development. Dochon synthesis society welfare facilities is openable to people by space that nature and human contacts and accomplishes relation, Made efforts to do so that can do part that causes human's ripple effect of contact with human and accomplishes traffic and recovers identity of Dochon area at the same time recovery of community consciousness.

• 복식
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• 제59권2호
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• pp.111-127
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• 2009

The purpose of this study is to understand the importance of exterior influential factors and kunstwollen(artistic will) of fashion designer. In order to investigate the differences which based on periodic backgrounds, social influences, cultural actualities and kunstwollen, Vivienne Westwood, Issey Miyake and Hussein Chalayan who have different social and cultural contexts are selected. Also through this research, people would understand the clothes has the values as the art works which have philosophy of creator and recognize the importance of fashion as an intermediation for comprehension of history, society and culture. Vivienne Westwood presents her honor to British history and culture in the way of her unique interpretation. Also she expresses the authority and the pride of woman who lives in the present age. Issey Miyake accomplishes the harmony of human body and soul. He attains the most perfect beauty through incomplete beauty of cloth. Hussein Chalayan produces his fashion works as a form of concept which is keep asking question about existence of human being. Fashion is the work of art which Is based on the relationship between clothes, human body and spirit. The fashion designer makes human life has value through his or her creations and tries to communicate with the world.

• 대한음성학회:학술대회논문집
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• 대한음성학회 1996년도 10월 학술대회지
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• pp.378-378
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• 1996

Human beings and chimpanzees are very much alike. and scientists say there is only 1% difference between them. Contrary to our expectations, the difference lies not in brains but in tracheas ( windpipes ). Those of human beings are bigger and longer than those of chimpanzees. Thu means more air is inspired and expired as breath. About breath there are interesting descriptions in the Bible. In the Genesis it says God made a man out of soil and breathed life-giving breath into his nostrils and the man began to live. In other part it says life exists between incoming breath and outgoing breath. Thus breath plays key role is our life. In Hebrew and Greek, breath and spirit are the same words. In Hebrew it is ‘Luahf’ and in Greek, ‘Pneuma’ With breath and mouth organs human beings produced voice, and with haritage and through leaning we train our voice to reach the level of language which convey our culture. My contention is to realize the gift of voice and train it so that it can perform proper function as a tool of conveying our thought and culture. This is a kind of practice of speech and it may be called speechology. It includes the following practical methods: 1. Try to read aloud. 2. Encourage recitation, 3. Make public speaking as possible. 4. Learn theories of phonetics; such as about pronunciation, accent, intonation, prominence, assimilation and so on.

• 한국식품영양과학회지
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• 제24권3호
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• pp.362-370
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• 1995

Human plasma low density lipoprotein(LDL) is the main carrier for cholesterol, and recent studies suggest the normal LDL can be readily oxidized by free radical and not interact with LDL receptor. Lipoprotein pariticles are consisted of lipid andprotein, and fatty acids of lipoproteins are prone to oxidation. LDL particles readily undergo oxidative modification by copper. From the results, oxidized LDL altered its biological properties. A marked increase in the electrophoretic mobility of LDl on agarose gel indicated that negative surface charge of the LDL particles was increased. Also, the results from the HPLC showed that oxidized LDL was degraded into several polypeptides nonenzymatically. Degradation tests which measured the amount of 5-IAF labelled oxidized LDL were carried out by monocyte and hepatocyte cell culture. Hepatocyte cell culture of modified LDL did not show consistent pattern. However, binding rate of modified LDL with HMDM(human monocyte derived macrophage) was enhanced with oxidation, but was retarded by addition of antioxidants(hyaluronic acid, vitamin A, vitamin E). Also comparisons of oxidized-LDL, acetyl-LDL and MDA-LDL showed significant differences in the chemical properteis and binding affinity to HMDM. Thus, modificaition of normal LDL altered its biological properties.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.690-693
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• 2000

Efficient intron deletion with the correct splicing of the two exons of the human proinsulin gene was accomplished by a novel stepwise method using genomic DNA [5]. The two exons were separately amplified in two steps, using the second step primers that incorporated additional bases complementary to the other exon. The fragments were combined in a third PCR reaction. Cloning and sequencing of the PCR product demonstrated the correct splicing of the two exons. Expression studies, using the pET9a vector, revealed a protein band with the correct size with respect to human proinsulin as confirmed by SDS-PAGe and Western blot. Proinsulin concentration was estimated to be around 200 mg per liter culture, expressed as inclusion bodies. Protein secretion to the culture medium and periplasmic space was achieved by cloning in the pEZZ18 vector.

• 한국식생활문화학회지
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• 제11권1호
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• pp.71-82
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• 1996

This study understands the actual conditions and problems for making traditional Korean food into the fast food. 1. Convenience to eat and nice atmosphere with friends (25%) are the best motive of visiting fast food restaurant. 2. The frequency of eating fast food is one to three times a month and the result of analysis on interrelation of human being in social anthropology represents that the fast food represents negative (-) interrelation by increasing of the age. The opinion about the fast food's nutrition is that the fast food lacks the nutrition (23.3%) and has excessive fat (40%). 3. The consumers were satisfied with the atmosphere of the fast food restaurant and unsatisfied with it's high prices. 4. The consumers' opinion on making the traditional food into a fast food was 66.4%. The consumers' opinions on traditional food to be improved are the sanitation (37.9%), the cookery (25%), the taste (11.7%), the nutrition (10.7%) and the opinions about inheritance of the traditional food is that 87.5% of people gave positive responses.

• Food Science and Biotechnology
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• 제15권3호
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• pp.458-461
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• 2006

To determine whether the toxicity of Bacillus cereus would be seen in human cell lines and mice, we screened B. cereus B-38B, B. cereus B-50B, and B. cereus KCCM40935 for genes that coded for 5 enterotoxins using the polymerase chain reaction and cultivated them for 17 hr, by whose time they had grown to $10^7-10^8$ colony-forming units (CFU) per milliliter. Cell-free supernatant was added to make up 1% of the total reaction solution. Human cells from normal lung, lung carcinoma, embryonic kidney, and cervical adenocarcinoma cell lines were grown in culture. The cytotoxicity induced by adding the reaction solution was indicated by cell death rates of 0 to 70%, depending on the bacterial strain involved and the cell line. A lethality of 20% was observed when B. cereus cultures containing $10^7-10^8$ viable cells were administrated orally to mice. Therefore, the culture of B. cereus containing $10^7-10^8$ viable cells seems to have high cytotoxicity on human cell lines and lethality on mice.