• Animal cells and systems
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• 제5권2호
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• pp.133-137
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• 2001

Long terminal repeats (LTRs) of the human endogenous retrovirus K family (HERV-K) have been found to be coexpressed with sequences of genes closely located nearby. We examined transcribed HERV-K LTR elements in human brain tissue. Using cDNA synthesized from mRNA of the human brain, we performed PCR amplification and identified ten HERV-K LTR elements. These LTR elements showed a high degree of sequence similarity (92.4-99.7%) with the human-specific LTR elements. A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-K LTR elements could be divided into two groups through evolutionary divergence. Some HERV-K LTR elements (HKL-B7, HKL-B8, HKL-B10) belonging to the group II from human brain cDNA were closely related to the human-specific HERV-K LTR elements. Our data suggest that HERV-K LTR element are active in the human brain; they could conceivably play a pathogenic role in human diseases such as psychosis.

• Molecules and Cells
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• 제40권9호
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• Molecules and Cells
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• 제27권3호
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• pp.313-319
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• 2009

The dual-vector system-II (DVS-II), which allows efficient display of Fab antibodies on phage, has been reported previously, but its practical applicability in a phage-displayed antibody library has not been verified. To resolve this issue, we created two small combinatorial human Fab antibody libraries using the DVS-II, and isolation of target-specific antibodies was attempted. Biopanning of one antibody library, termed DVFAB-1L library, which has a $1.3{\times}10^7$ combinatorial antibody complexity, against fluorescein-BSA resulted in successful isolation of human Fab clones specific for the antigen despite the presence of only a single light chain in the library. By using the unique feature of the DVS-II, an antibody library of a larger size, named DVFAB-131L, which has a $1.5{\times}10^9$ combinatorial antibody complexity, was also generated in a rapid manner by combining $1.3{\times}10^7$ heavy chains and 131 light chains and more diverse anti-fluorescein-BSA Fab antibody clones were successfully obtained. Our results demonstrate that the DVS-II can be applied readily in creating phage-displayed antibody libraries with much less effort, and target-specific antibody clones can be isolated reliably via light chain promiscuity of antibody molecules.

• 정보관리학회지
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• 제36권1호
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• pp.73-94
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• 2019

이 연구는 지방연구원 소속 전문도서관의 서비스 품질 평가를 통하여 실제 이용자 만족에 영향을 주는 서비스 품질을 파악하고 전문도서관의 기능과 역할을 더욱 효율적으로 개선하기 위한 방향을 제시하고자 하였다. 이를 위해 지방연구원 소속 6개의 전문도서관 이용자 111명을 대상으로 LibQUAL+을 기반으로 수정된 설문조사와 심층인터뷰를 실시하였다. 연구결과, 인력품질이 공간품질과 정보품질 보다 전반적 만족도에 미치는 영향이 더 큰 것으로 확인되었고, 서비스 품질 하위변수 중에서는 인력품질의 서비스 태도, 공간품질의 공간 편의성, 정보품질의 정보 유용성이 이용자의 전반적 만족도에 영향을 미치는 것으로 나타났다. 인터뷰 결과로 자료부족, 디지털 자료의 비활성화, 도서관 공간개념의 환경, 타 기관과의 자료공유 비활성화, 인력부족 등의 문제점이 지적되었다. 이 연구는 이러한 분석 결과를 바탕으로 지방연구원 소속 전문도서관의 서비스 품질에 대한 개선방안을 제시하였다.

• 한국주거학회논문집
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• 제14권3호
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• pp.127-136
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• 2003

The purpose of this study is to establish concept about a plan direction of preschooler's library, and provide a design type of preschooler's library. As a result, the following findings were obtained: 1) For the preschooler's reading area, it should be segregated between schooler and preschooler. Because there is a difference between a library use action and a development characteristic, it needs to be segregated to avoid conflict using library for both of those groups. 2) The space composition of preschooler's library stands up, and of a tube considers a traffic line of a user according to the function and systematizes a child, and to cause an interest of preschoolers. 3) Furnitures and equipment of preschooler's library must arrange in consideration of human body size of a user in a tube.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2005년도 추계학술발표대회 논문집
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• pp.219-220
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• 2005

This present study purposed to propose a plan of building a future oriented school library that supports teachers' teaching with various multimedia and Internet materials applicable in class immediately, helps students' self directed learning activities, and plays its role as a cultural center of the local community. As for research methods, this study examines the basic directions and characteristics of school libraries defined by the Ministry of Education and Human Resources Development, and investigates the current state of school libraries. In addition, we survey cases of library plans in Incheon, and suggest standards for planning and propose a plan. Ultimately, we establish a library plan for S Elementary School in Incheon and execute the plan and, in the course, derive ideas for satisfying students' demands with regard to their sentiment, stable and flexible layout and flow lines, facilities and space within the library, etc. What is more, we create indexes for library planning so that school libraries can contribute to various cultural activities and the development of local community beyond their functions as school library facilities.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.377-381
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• 2012

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.829-832
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• 2001

Phage antibody 생산방법은 유전자 조작에 의하여 phage coat에 항체를 발현시키는 방법이며 그 library를 이용하여 일반 hybridoma 방법에 의해 항체를 생산하기 어려운 성분 (예: 독성물질, 면역화가 잘 안되는 물질 등) 에 대해 적용할 수 있는 장점을 제공한다. 본 연구진은 Griffin.1 antibody library의 positive control을 통해 phage 표면에 항체가 발현되는 지의 여부를 ELISA test를 통하여 확인하였다. 또한 human serum albumin을 모델분석물질로 사용하여 $1{\sim}4$차까지의 bio-panning 을 실시하였고 각 단계에서 증폭된 phage를 가지고 ELISA test를 한 결과 신호가 점진적으로 증가하는 data를 얻을 수 있었다. 이것을 통해 phage library로부터 특정 항원에 대한 monoclonal antibody를 생산할 수 있다는 사실을 검증하였다.

• 한국문헌정보학회지
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• 제38권3호
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• pp.277-296
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• 2004

본 논문에서는 대학 도서관 경영환경의 변화가 정보서비스에 미치는 영향을 분석하였다. 이를 위해 문헌적 연구를 바탕으로 인적자원, 정보자원, 시설, 예산, 정보기술의 제 영역에서 경영환경 변화에 따른 정보서비스에 영향을 미치는 요인들을 살펴보았다. 또한 실증적인 검증을 위하여 대학 도서관에 근무하고 있는 사서들을 대상으로 설문 조사를 실시하였다. 그 결과. 대학도서관이 정보서비스를 제공하는데 있어서 첫째, 인적자원 요인, 둘째, 정보기술 요인, 셋째, 시설 요인 순으로 많은 영향을 미치고 있는 것으로 조사되었다.

• 한국도서관정보학회지
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• 제36권1호
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• pp.459-477
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• 2005

본 연구의 목적은 인간들이 공통적으로 공유하는 지식구조를 기반으로 온톨로지를 구축하기 위해 인간들이 가지고 있는 개념들의 범주와 관계를 조사하여 기본모델을 제시하고자 하는데 있다. 연구방법은 1차 조사에는 설문지법을 이용하였고 2차 조사에는 면담법을 사용하였다. 연구 결과 문헌정보학에 대한 KDC나 DDC의 구조와 학생들이 가지고 있는 지식구조에는 차이가 있었다. 학생들이 가지고 있는 구조의 기본 틀은 개체중심의 관계성으로 이루어져 있었다. 조사결과 4가지의 관계성 모델이 축출되었는데, 이 모델은 문헌정보학 분야 온톨로지 구축에 기초 자료로 활용할 수 있을 것이다. 그리고 학업성적이 지식구조에 영향을 미치고 있는 것으로 밝혀졌다.