Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Screening Peptides Binding Specifically to Colorectal Cancer Cells from a Phage Random Peptide Library

  • Wang, Jun-Jiang (Department of Gastroenterology Surgery, Guangdong General Hospital) ;
  • Liu, Ying (Department of Obstetrics and Gynecology, Guangdong General Hospital) ;
  • Zheng, Yang (Department of Gastroenterology Surgery, First Affiliated Hospital of Shan-tou University Medical College) ;
  • Liao, Kang-Xiong (Department of General Surgery, the Jieyang People's Hospital) ;
  • Lin, Feng (Department of Gastroenterology Surgery, Guangdong General Hospital) ;
  • Wu, Cheng-Tang (Department of General Surgery, Nanfang Hospital, Southern Medical University) ;
  • Cai, Guan-Fu (Department of Gastroenterology Surgery, Guangdong General Hospital) ;
  • Yao, Xue-Qing (Department of Gastroenterology Surgery, Guangdong General Hospital)
  • Published : 2012.01.31
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Abstract

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.

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