• 대한의생명과학회지
• /
• 제23권3호
• /
• pp.272-276
• /
• 2017

HOX genes are transcription factors that play important roles in body patterning and cell fate specification during normal development. In previous study, we found aberrant overexpression of HOXB5 in breast cancer tissues and cell lines, and demonstrated that HOXB5 is important in regulation of cell proliferation, tamoxifen resistance, and invasiveness through the epithelial-mesenchymal transition (EMT). Although the relationship between HOXB5 and phenotypic changes in MCF7 breast cancer cells has been studied, the molecular function of HOXB5 as a transcription factor remains unclear. IL-6 has been reported to be involved in not only inflammation but also cancer progression, which is characterized by the increase of growth speed and invasiveness of tumor cells. In this study, we selected Interleukin-6 (IL-6) as HOXB5 putative downstream target gene and discovered that HOXB5 transcriptionally up-regulated the expression of IL-6 in HOXB5 overexpressing MCF7 cells. The upstream region (~1.2 kb) of IL-6 promoter turned out to contain several putative HOX consensus binding sites. Chromatin immunoprecipitation assay confirmed that HOXB5 directly binds to the promoter region of IL-6 and positively regulated the expression of IL-6. These data all together, indicate that HOXB5 promotes IL-6 transcription by actively binding to the putative binding sites located in the upstream region of IL-6, which enable to increase its promoter activity in MCF7 breast cancer cells.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권4호
• /
• pp.1349-1353
• /
• 2015

Background: Cervical cancer (CeCa) is the second most common cancer in women in developing countries, and human papilloma virus (HPV) is the primary etiological factor. Aberrant expression of HOX transcription factors has been observed in several types of cancer. To date, however, no reports exist on the expression of HOXB2 and HOXB13 proteins during neoplastic progression in CeCa and its correlation with HPV infection. Materials and Methods: Expression of HOXB2 and HOXB13 proteins was assessed in tissue microarrays from normal cervical epithelium, cervical intraepithelial neoplasias grade 1-3, and CeCa. HPV was detected by PCR and sequencing. Expression of HOX-positive cells was determined in each diagnostic group. Results: Percentage of HOXB2- and HOXB13-positive cells gradually increased from means of 10.9% and 16.7%, respectively, in samples from healthy women, to 75.2% and 88.6% in those from CeCa patients. Frequency of HPV infection also increased from 13% in healthy tissue samples to 92.3% in CeCa. Both HOXB2 and HOXB13 proteins were preferentially expressed in HPV+ samples. Conclusions: The present study represents the first report on the expression of both HOXB2 and HOXB13 proteins through cervix tumorigenesis, providing evidence that increased expression of such proteins is a common event during progression to CeCa.

• 대한수의학회지
• /
• 제35권1호
• /
• pp.95-105
• /
• 1995

The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR)- a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (${\beta}$-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern, Since the expression of ${\beta}$-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human.

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
• /
• pp.19-22
• /
• 2003

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We found that Drosaphiia FACT, a heterodimer of dSPT16 and dSSRPl, is associated with GAGA factor through its dSSRPl subunit, binds to a nucleosome and facilitates GAGA factor-directed chromatin remodeling. Immunostaining of polytene chromosomes revealed colocalization of GAGA factor and FACT in many specific loci. (omitted)

• 기술사
• /
• 제33권5호
• /
• pp.16-22
• /
• 2000

The rapid industrializing of China has helped the development of the industrial economy since 1980. However, the problem is some environmental pollution, such as SO$_2$, TSP, HOx, Dioxin etc, generated by the industrial facilities located in Northeastern China is moving to the Korean Peninsula through the cold air stream during the Asian dust season, This kind of air pollution worsens the environmental pollution of the Korean Peninsula year by year. Therefore, NGO suggests to China that the minimization of the environmental problems linked to both Korea and China has to be discussed. In fact, I would like to suggest that the activity of EMS(ISO14000) should help the environmentally sound '||'&'||' sustainnable development of both countries,

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
• /
• pp.213.3-214
• /
• 2001

No Abstract, See Full Text

• 대한기계학회논문집B
• /
• 제31권2호
• /
• pp.167-172
• /
• 2007

A new method that optimizes a control of hydrocarbon (HC) addition to diesel exhaust gas for HC type DeNOx catalyst system has been developed. These catalysts are called the HC-DeHOx catalyst in this paper. The system using HC-DeNOx catalyst requires a resonable quantity of hydrocarbons addition in the inlet gas of the catalyst, because the HC concentration in a diesel engine is so low that the HC is not sufficient for NOx conversion. It is expected that this study offers a robust data developing HC injection system.

• Clinical and Experimental Reproductive Medicine
• /
• 제31권4호
• /
• pp.217-223
• /
• 2004

Objective: To evaluate the effects of recombinant FSH (rFSH) and urinary FSH (uFSH) on the gene expressions of human endometrial stromal cells in vitro. Methods: Endometrial tissue was obtained from a pre-menopausal women undergoing hysterectomy. Primary endometrial stromal cells were isolated and in vitro cultured with FBS-free DMEM/F-12 containing 0, 10, 100, and 1, 000 mIU/ml of rFSH and uFSH for 48 hours, respectively. Total RNA was extracted from the cultured cells and subjected to real time RT-PCR for the quantitative analysis of progesterone receptor (PR), estrogen receptor $\alpha/\beta$ (ER-$\alpha/\beta$), cyclooxygenase 2 (Cox-2), leukemia inhibitory factor (LIF), homeobox A10-1 and -2 (HoxA10-1/-2). Results: Both hormone treatments slightly increased (< 3 folds) the expressions of PR, ER-$\beta$ and HoxA10-1/-2 gene. However, ER-$\alpha$ expression was increased up to five folds by treatments of both FSH for 48 hours. The LIF expression by the 10 mIU/ml of uFSH for 12 hours was significantly higher than that of rFSH (p<0.01). After 24 hours treatment of two kinds of hormones, the expression patterns of LIF were similar. The 100 and 1, 000 mIU/ml of rFSH induced significantly higher amount of Cox-2 expression than those of uFSH, respectively (p<0.05). Conclusion: This study represents no adversely effect of exogeneous gonadotropins, rFSH and uFSH, on the expression of implantation related genes. We suggest that rFSH is applicable for the assisted reproductive technology without any concern on the endometrial receptivity.

• 한국작물학회지
• /
• 제49권6호
• /
• pp.447-451
• /
• 2004

분무경 양액재배로 얻어진 비절단씨감자 및 $2\~4$절 절단씨감자를 심지양액재배상에 파종하여 생육 및 수량형질변화를 조사함으로서 상위급 씨감자의 안정적 대량생산가능성을 조사하였다. 감자 심지 양액재배시스템은 순환식으로 4열 2조로 제작하였으며, 성형베드 안쪽에 크기가 $31(W)\times20(D)\times51(L)cm$인 폴리스티렌상자$(0.031m^3)$를 올려놓고 그 안에 8개의 심지(폭 1.5cm, 길이 40cm)를 꼽고 porlite+peatmoss(1:2, v/v) 배지를 채운 후 식물을 재배할 수 있도록 하였다. 지상부 주당생체중은 씨감자의 절편무게가 10g에서 30g으로 증가할수록 생체중도 증가하였다. 종서로 이용이 가능한 주당상품괴경수량(5g 이상)은 비절단씨감자의 무게가 증가할수록 82.3g에서 140.1g으로 무거워졌고, 절단씨감자처리는 10g 4절 처리를 제외한 모든 시험구에서 5g 비절단씨감자 처리와 비슷하거나 많았으며, 20g 2절과 30g 2절, 4절 처리가 100g내외의 괴경수량을 보였다. 절단씨감자 처리는 $m^2$당 총괴경수량이 $4.16\~6.56kg$범위에 있었고, 절편무게가 7.5g 이상(20g 2절 및 30g 2절, 4절)은 5.6kg 이상의 총괴경수량을 보였으며, 주당상품괴경수량(5g이상)의 비율은 모든 처리에서 $97\%$ 이상으로 높게 나타났다. 결론적으로 분무경 양액재배의 단점을 보완할 수 있는 심지 양액재배시 분무경으로 생산한 $10\~20g$크기의 소괴경은 2절로, 20g이상의 소괴경은 4절로 각각 5g내외로 절단하여 이용할 경우 상위급 씨 감자의 친환경적인 대량생산이 가능할 것으로 판단된다.

• Animal Systematics, Evolution and Diversity
• /
• 제17권2호
• /
• pp.263-275
• /
• 2001

전통적인 동물의 계통수는 초기발생 형질들에 기초하여 몸 구조의 복잡성에 따라 동물문들을 단순한 동물에서 복잡한 동물 순으로 점진적으로 배열하는 것인데 현재 활발히 연구되는 분자계통 연구들이 이 경향을 재평가하고 새로운 계통과 의미를 제시하고 있다. 주로 18S rRNA 에 의한 분자계통에 의하면 무체강동물과 의체강동물과 같이 진체강동물로의 전이단계에 위치했던 무리들이 서로 나뉘어진 진체강동물 안에 속하게 된다. 이러한 새로운 분자계통은 좌우대칭동물의 공동조사에 대한 새로운 가정들을 세울 수 있는 뼈대와 비교발생학 및 비교유전체학 정보가 해석될 수 있는 토대를 제공한다.