• Horticultural Science & Technology
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• v.32 no.3
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• pp.408-414
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• 2014

The antimicrobial and antioxidant activities of different cultivars and parts of broccoli were investigated. The screening of antimicrobial activities for the floret and leaf extracts of 11 cultivars against selected gram positive (Staphylococcus aureus and Listeria monocytogenes) and gram negative bacteria (Escherichia coli O157:H7 and Salmonella enteritidis) were conducted. The AMG cultivar showed the highest antimicrobial activity against S. aureus with the floret and leaf extracts (12.83 and 13.00 mm). Antibacterial activity against L. monocytogenes was strongest with floret extract of NJ cultivar (13.58 mm) and leaf extract of YDR cultivar (13.92 mm). Moreover, the size of inhibition zone against L. monocytogenes was bigger than those of 4 kinds of pathogenic bacteria. Both floret and leaf extracts of Grd cultivar showed the highest antimicrobial activity against E. coli O157: H7, but there was no difference between floret and leaf extracts. Floret extract o f NJ cultivar and leaf extract of NY and 0c cultivars were effective against S. enteritidis whereas leaf extract exhibited better antimicrobial effect than floret extract. These results showed that floret extract of NJ and leaf extract of 0c had the highest antioxidant activity which was 39.90 and 43.64%, respectively. The antioxidant activity of leaf extract was 1.5 times higher than that of floret extract. All cultivars except NJ showed that electron donating ability of leaf extract was higher than that of floret extract.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.29-37
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• 2017

The effect of packaging materials on the quality and physiological activity of Angelica acutiloba leaves during storage at $4^{\circ}C$ for 20 days and after being transferred to room temperature for 2 days was studied. The experiment was conducted using three packaging materials: the corrugated box (control); corrugated box with $20{\mu}m$ HDPE perforated film liner; and corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner. Changes in weight, color, aroma, overall visual quality, antioxidant activity, and total flavonoids were investigated. Based on the result, the treatments using corrugated box with $20{\mu}m$ HDPE perforated film liner and the corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner, were effective in minimizing weight loss, and maintaining external color and overall quality as compared to corrugated box only (control). The corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner was more effective in maintaining antioxidant activity and total flavonoids than other treatments. Prolonging the freshness using corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner could increase the marketability of Angelica acutiloba leaves in the domestic market.

• Journal of People, Plants, and Environment
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• v.23 no.4
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• pp.475-484
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• 2020

Background and objective: Horticultural activity is one of the most basic elements of horticultural therapy, which brings about therapeutic effects for participants through various plant-related activities. The main objective of this study was to verify the results of previous research, which suggested six types of activities from the exploratory factor analysis. Methods: To meet the purpose of this study, a questionnaire was designed to determine the preferences for 6 types of the horticultural therapy activities. The survey was conducted on 703 people from March 7 to June 20, 2019. The data of 674 cases were used into the final analysis, excluding unreliable responses. Descriptive statistics, and reliability analysis were performed using IBM SPSS Statistics 25, and confirmatory factor analysis was performed using IBM SPSS Amos 21. Results: First, horticultural therapy activities were classified into 6 types from the exploratory factor analysis, as conducted in previous research. The confirmatory factor analysis provided that the fit of the final model was satisfactory with χ² = 1,300.590 (p < .001), RMR = .045, GFI = .876, RMSEA = .062, NFI = .914, TLI = .905, CFI = .914. Conclusion: This result revalidated that the mode with 6 types of horticultural therapy activities from previous research is appropriate criteria for the classification of horticultural activities. The model could be used to design more systematic horticultural therapy programs that meet the needs or circumstances of the subject, or that are suitable for necessary therapeutic intervention methods.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.467-473
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• 2011

Flavonoid are large group of the polyphenolic compounds which are distinguished by an aromatic or phenolic ring structure and the phenolic compounds are induced by microbial infection, ultraviolet radiation, temperature and chemical stress. They are known for their antioxidant activity, anti-allergic, anti-inflammatory, anti-microbial and anti-cancer activities. In this study, changes in flavonoid content were investigated using heterologous chalcone isomerase (CHI) expression system. Also, phenolic compounds level was measured to examine the relation between flavonoids and phenols contents. Explants of lettuce (Lactuca sativa L.) were transformed with Agrobacterium tumefaciens LBA 4404 strain containing pFLH-CHI (derived from pPZP2Ha3) vector constructed with CHI gene from Brassica rapa. The putative transgenic plants were confirmed by genomic DNA PCR analysis. Also the transcription levels of the gene were analyzed by semi-quantitative RT-PCR with gene specific primers. The total flavonoid contents were increased at $T_0$ and $T_1$ generations over 1.4 and 4.0 fold, respectively. Total phenol contents also increased at $T_1$ generation. These results indicate that CHI gene plays an important role to regulate the accumulation of flavonoids and its component changes.

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.290-291
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• 2011

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.293-297
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• 2015

This study was conducted to evaluate the influence of feeding leaf positions on the growth, net formation of fruits, and occurrence of leaf yellowing symptoms (LYS) in muskmelon plants. Plants having five or ten more leaves above the fruit-bearing node produced the greater biomass than those of plants having equal or five less leaves above the fruit-bearing node. The number of leaves above the fruit-bearing node also influenced on the occurrence of LYS. The number of plants with LYS decreased as the number of leaves borne on the nodes above the fruit-bearing node increased. The LYS infected ratio of BL-5 treatment were the greatest, while fruit weight of BL+5 treatment were the greatest among all the tested treatments. In addition, the net formation of BL-5 treatment showed the poorest. Results indicated that maintaining the higher number of leaves over the fruit-bearing node might be feasible the practical method for coping physiological damages from yellowing symptoms.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.710-720
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• 2014

Movements of the upper and lower limb muscles during five common gardening tasks were analyzed by using electromyography (EMG). Twenty adults aged in their twenties (mean age, $24.8{\pm}2.4$ years) were recruited. On two separate occasions, subjects visited a garden plot to perform digging, raking, troweling, weeding, and hoeing; all tasks were performed three times with 20 s intervals for each trial. To measure muscle activation during the five gardening tasks, surface EMG was used. Bipolar surface EMG electrodes were attached to eight upper limb muscles (bilateral anterior deltoid, biceps brachialis, brachioradialis, and flexor carpi ulnaris) or eight lower limb muscles (bilateral vastus lateralis, vastus medialis, biceps femoris, and gastrocnemius) on both sides of the body, for a total of 16 muscles. During the five tasks, photographs were taken of movement phases using a digital video camera. The right flexor carpi ulnaris and brachioradialis showed higher activation than the other upper and lower limb muscles measured during the tasks. All 16 upper and lower limb muscles were actively used only during digging. According to movement analysis of each activity, digging was classified into four movement phases, whereas raking, troweling, weeding, and hoeing each were divided into three movement phases. In each activity, there were high-impact phases in terms of muscle activation; the flexor carpi ulnaris and brachioradialis were identified as major muscles in each impact phase. This analysis may be used to generate biomechanical profiles of gardening tasks for practitioners when designing efficient gardening interventions for physical health or rehabilitation.

• Journal of People, Plants, and Environment
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• v.23 no.3
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• pp.277-291
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• 2020

Background and objective: The purpose of this study is to investigate the effects of visitors' positive emotions on satisfaction and loyalty with the perception of restorative environment of a healing garden created in an urban agriculture expo. Methods: The psychological indicators to the images of the healing garden were analyzed by the visitors' demographic variables and the three factors of plant cultivation activity level: plant cultivation experience, plant preference, and plant-related event. Results: Between age groups and occupational groups, significant differences were found statistically. The Perceived Restorativeness Scale(PRS) showed significantly differences between age groups in repose, fascination and legibility. The Positive Affect & Negative Affect Schedule(PANAS) showed statistically significant differences between age groups in positive emotions. In addition, we investigated the correlation between the PANAS and the three factors of plant cultivation experience level, the four factors of the PRS, satisfaction and loyalty. The three factors of plant cultivation experience level, the four factors of the PRS, satisfaction and loyalty showed a positive correlation with positive emotions and were inversely correlated with negative emotions significantly. Multiple regression analysis with dummy variables was conducted to examine the effects of plant cultivation activity level, attention restoration, and the PANAS on healing garden visitors' satisfaction and loyalty. As a result, among the four factors of the PRS, fascination and positive affectivity were significant variables that affect healing garden visitors' satisfaction and loyalty. Conclusion: The results indicated that the higher the attention restoration of visitors due to the fascination of the healing garden and the higher their positive affectivity and the more they have plant-related memories, the higher their impact on healing garden visitors' satisfaction and loyalty. Therefore, fascinating natural environments or greenery landscapes like healing gardens where people can contact plants would reduce negative emotions such as anger and sadness but to increase positive emotions such as pleasure, joy and satisfaction.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1169-1178
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• 1998

This study was performed to purify fig pectinesterase(F-PE) and characterize its in situ activity. Three kinds of F-PE were partially separated by using ammonium sulfate fractionation, Q-Sepharose column, CM-cation exchanger column chromatography, and HPLC. One of those was anionic protein and the others were cationic proteins. All of them had approximate molecular weight of 27,000 and lost rapidly their activity during storage. Therefore alternative crude enzyme was prepared by suspending the freeze dried and milled fig powder in 0.1 M NaCl at pH 7.5. F-PE had the optimum pH of 8.5, the optimum temperature of $50^{\circ}C$ with activation energy of 7,671 cal $mol^{-1}K^{-1}$ and stability up to $55^{\circ}C$ with 10 minutes heating. Optimum activity was obtained in $0.2{\sim}0.4$ M NaCl with optimum solubility at above 0.8 M NaCl.

• Journal of Applied Biological Chemistry
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• v.57 no.4
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• pp.359-363
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• 2014

The roots of Lythrum salicaria L. were extracted in 80% aqueous MeOH and the concentrated extract was fractionated with EtOAc, n-BuOH, and $H_2O$, successively. The repeated silicagel and octadecyl $SiO_2$ column chromatographies of the EtOAc fractions led to isolation of an antioxidant compound and two major compounds. From the results of spectral data and the chemical characteristics including nuclear magnetic resonance, MS, and IR, the structures of compounds were determind as myricetin-3-O-${\beta}$-D-glucopyranoside (1), oleanolic acid (2), betulinic acid (3). This is the first reported isolation of compounds (1, 2) from L. salicaria. Compound 1 as well as EtOAc, n-BuOH, and $H_2O$ solvent fractions were evaluated for 2,2-dipicryl-1-phenylhydrazyl radical scavenging activity.