Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Antioxidant Compound from the Lythrum Salicaria L. Roots

털부처꽃(Lythrum Salicaria L.) 뿌리로부터 항산화 물질의 분리 및 구조동정

  • Lee, Kyeong-Hee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Noh, Hyung-Jun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Jeong-Hoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Jehun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Chun-Geun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Seung-Yu (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Jun-Su (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2014.05.19
  • Accepted : 2014.08.11
  • Published : 2014.12.31
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Abstract

The roots of Lythrum salicaria L. were extracted in 80% aqueous MeOH and the concentrated extract was fractionated with EtOAc, n-BuOH, and $H_2O$, successively. The repeated silicagel and octadecyl $SiO_2$ column chromatographies of the EtOAc fractions led to isolation of an antioxidant compound and two major compounds. From the results of spectral data and the chemical characteristics including nuclear magnetic resonance, MS, and IR, the structures of compounds were determind as myricetin-3-O-${\beta}$-D-glucopyranoside (1), oleanolic acid (2), betulinic acid (3). This is the first reported isolation of compounds (1, 2) from L. salicaria. Compound 1 as well as EtOAc, n-BuOH, and $H_2O$ solvent fractions were evaluated for 2,2-dipicryl-1-phenylhydrazyl radical scavenging activity.

털부처꽃(Lythrum salicaria L.) 뿌리는 실온에서 80% MeOH로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. 2,2-dipicryl-1-phenylhydrazyl (DPPH) radical 소거능을 이용한 항산화 활성을 측정하여 활성 추적 분획(Activity guided fractionation)에 따라 항산화 활성이 높은 EtOAc 분획에 대하여 silica gel 및 octadecyl $SiO_2$ column chromatography를 반복하여 항산화 활성을 나타내는 화합물 1과 major 화합물 2 및 3을 분리, 정제하였다. NMR, IR, 및 MS 등의 spectrum을 해석하여, Myricetin-3-O-${\beta}$-D-glucopyranoside (1), oleanolic acid (2) 및 betulinic acid (3)로 구조를 결정하였다. 화합물 1은 DPPH radical 소거능을 이용한 항산화 활성을 측정 한 결과 $25{\mu}g/mL$ 농도에서 $74.47{\pm}1.64%$의 저해 값을 가지는 것으로 나타났다.

Keywords

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