• 생약학회지
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• 제34권4호통권135호
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• pp.344-351
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• 2003

Phytochemical examination of seeds of Astragalus sinicus has led to the isolation and characterization of kaempferol $3-O-{\beta}-D-apiofuranosyl-(1{\rightarrow}2)-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), quercetin $3-O-{\beta}-D-xylopyranosyl-(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (2), ampelopsin (3), ampelopsin $3'-O-{\beta}-D-xylopyranoside$ (4), ampelopsin $3'-O-{\beta}-Dxylopyranoside$ (5), myricetin (6), myricetin $3'-O-{\beta}-D-glucopyranoside$ (7), myricetin $3'-O-{\beta}-D-xylopyranoside$ (8). Antioxidative activity of these compounds was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Flavanonols(3,4, and 5) showed potent antioxidative activity.

• 생약학회지
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• 제25권3호
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• pp.199-203
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• 1994

Chemical examination of the leaves of Betula platyphylla var. latifolia has led to the isolation and characterization of five flavonoid glycosides including two C-glucosyl flavonoids. The structures of these compounds were elucidated as myricetin $3-O-{\alpha}-_L-rhamnoside$ (myricitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (isoquercitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (hyperoside), $nalingenin-6-C-{\beta}-_D-glucopyranoside$ (hemiphloin) and $aromadendrin-6-C-{\beta}-_D-glucopyranosidre(6-C-glucosyldihydrokaempferol)$ on the basis of physico-chemical and spectroscopic evidences.

• Journal of the Korean Wood Science and Technology
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• 제34권3호
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• pp.73-83
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• 2006

구상나무(Abies koreana Maximowicz)와 전나무(Abies holophylla Wilson) 잎을 채취하여 건조시킨 후 분말로 제조하여 각각 1.5 kg을 아세톤-물(7:3, v/v)로 추출하고 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 분획하여 동결건조 시켰다. 에틸아세테이트용성 분획을 Sephadex LH-20으로 충진한 칼럼에서 메탄올과 에탄올-헥산 혼합액을 용리용매로 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 $^1H-$, $^{13}C-NMR$, COSY, HETCOR 등의 스펙트럼을 사용하여 정확한 구조를 규명하였고 FAB 및 EI-MS로써 분자량을 측정하였다. 많은 양의 aromadendrin-7-O-${\beta}$-D-glucopyranoside (화합물 III), polydatin (화합물 VI), (-)-rhododendrol-2-O-${\beta}$-D-glucopyranoside (화합물 VII)가 단리되었으며, 소량의 (+)-catechin (화합물 I), kaempferol-3-O-${\beta}$-D-glucopyranoside (화합물IV), myricetin-3-O-${\beta}$-D-glucopyranoside (화합물 V), naringenin-7-O-${\beta}$-D-glucopyranoside (화합물 II)도 단리 되었다. DPPH 라디칼 소거법을 이용하여 단리된 화합물들에 대한 항산화 활성시험을 실시하였으며 (+)- catechin과 polydatin이 항산화 효능을 나타내었다.

• 생약학회지
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• 제36권2호통권141호
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• pp.121-128
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• 2005

In order to find the antioxidative compounds, fractionation of the MeOH extract of the leaves of Crataegus pinnatifida guided by DPPH scavenging test furnished seven phenolic compounds, $quercetin-3-O-{\alpha}-L-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), myricetin-3-O-rhamnose (2), $quercetin-3-O-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-galctopyranoside$ (3), $quercetin-3-O-{\beta}-D-galactopyranoside$ (4), quercetin (5), $apigenin-8-C-{\beta}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (2'-O-rhamnosylvitexin) (6) and (-)-epicatechin (7). All of isolated compounds showed the significant antioxidative effect on DPPH free radical scavenging test and TBARS assay.

• 생약학회지
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• 제38권1호
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• pp.76-83
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• 2007

In this study, three triterpenoids, two steroids and nine flavonoids were isolated from the leaves of Alnus firma Sieb. et Zucc. On the basis of spectroscopic evidences, the structures of these compounds were established as ${\beta}$-amyrin acetate, ${\beta}$-amyrin, ${\beta}$-sitosterol, alnustic acid methyl ester, ${\beta}$-sitosterol glucoside, pinocembrin, alnustinol, quercetin, quercetin-3-O-${\alpha}$-L-arabinofuranoside, quercetin-3-O-${\alpha}$-L -rhamnopyranoside, quercetin-3-O-${\beta}$-D-glucopyranoside, myricetin-3-O-${\beta}$-D-galac-topyranoside, (+)-catechin and (-)-epicatechin.

• Journal of Applied Biological Chemistry
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• 제57권4호
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• pp.359-363
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• 2014

털부처꽃(Lythrum salicaria L.) 뿌리는 실온에서 80% MeOH로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. 2,2-dipicryl-1-phenylhydrazyl (DPPH) radical 소거능을 이용한 항산화 활성을 측정하여 활성 추적 분획(Activity guided fractionation)에 따라 항산화 활성이 높은 EtOAc 분획에 대하여 silica gel 및 octadecyl $SiO_2$ column chromatography를 반복하여 항산화 활성을 나타내는 화합물 1과 major 화합물 2 및 3을 분리, 정제하였다. NMR, IR, 및 MS 등의 spectrum을 해석하여, Myricetin-3-O-${\beta}$-D-glucopyranoside (1), oleanolic acid (2) 및 betulinic acid (3)로 구조를 결정하였다. 화합물 1은 DPPH radical 소거능을 이용한 항산화 활성을 측정 한 결과 $25{\mu}g/mL$ 농도에서 $74.47{\pm}1.64%$의 저해 값을 가지는 것으로 나타났다.

• 약학회지
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• 제44권1호
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• pp.41-46
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• 2000

Phytochemical examination of the leaves of Alnus maximowiczii Call, one of the indigenous Alnus species grows in Korea, has led to the isolation of three flavonoids. Structure of these flavonoids were elucidated as quercetin-3-O-$\beta$-D-glucopyranoside (1) , quercetin-3-O-$\alpha$-L-rhamnopyranoside (2) and myricetin-3-O-$\alpha$-L-rhamnopyranoside (3) respectively by means of physico-chemical and spectral evidences.