• Journal of Microbiology and Biotechnology
• /
• 제30권7호
• /
• pp.974-981
• /
• 2020

Sequence type 410 (ST410) of Escherichia coli is an extraintestinal pathogen associated with multi drug resistance. In this study, we aimed to investigate the horizontal propagation pathway of a high-risk clone of E. coli ST410 that produces Klebsiella pneumoniae carbapenemase (KPC). blaKPC-encoding E. coli and K. pneumoniae isolates were evaluated, and complete sequencing and comparative analysis of blaKPC-encoding plasmids from E. coli and K. pneumoniae, antimicrobial susceptibility tests, polymerase chain reaction, multilocus sequence typing, and conjugal transfer of plasmids were performed. Whole-genome sequencing was performed for plasmids mediating KPC-2 production in E. coli and K. pneumoniae clinical isolates. Strains E. coli CPEc171209 and K. pneumoniae CPKp171210 were identified as ST410 and ST307, respectively. CPEc171209 harbored five plasmids belonging to serotype O8:H21, which is in the antimicrobial-resistant clade C4/H24. The CPKp171210 isolate harbored three plasmids. Both strains harbored various additional antimicrobial resistance genes. The IncX3 plasmid pECBHS_9_5 harbored blaKPC-2 within a truncated Tn4401a transposon, which also contains blaSHV-182 with duplicated conjugative elements. This plasmid displayed 100% identity with the IncX3 plasmid pKPBHS_10_3 from the K. pneumoniae CPKp171210 ST307 strain. The genes responsible for the conjugal transfer of the IncX3 plasmid included tra/trb clusters and pil genes coding the type IV pilus. ST410 can be transmitted between patients, posing an elevated risk in clinical settings. The emergence of a KPC-producing E. coli strain (ST410) is concerning because the blaKPC-2-bearing plasmids may carry treatment resistance across species barriers. Transgenic translocation occurs among carbapenem-resistant bacteria, which may spread rapidly via horizontal migration.

• 대한치과보철학회지
• /
• 제40권1호
• /
• pp.18-29
• /
• 2002

As the inaccuracy which was made in implant impression prevented passive fit, it needed to solder the sectioned framework at several locations to correct the inaccuracy. Many clinicians have suggested impression techniques which could make passive fit between implant and superstructure. The purpose of this research was to measure and compare the accuracy of three methods of taking impression with the strain amplifier. The experimental groups were classified as follows : Group I : splinted the two parts with $Futar^{(R)}D$ Occlusion. Group II : splinted the two parts with $DuraLay^{(R)}$. Group III ; didn't splint the two parts. The results were as follows ; 1. The values of strain in the vertical and horizontal surfaces were increased in the order of group I, group II and group III. 2. Group 1 showed higher accuracy of the duplicated casts in the vortical and horizontal sur faces than group II and group III (p<0.05). 3. There were no significant differences in the accuracy of the duplicated casts between group II and group III. From the above results, it is considered that the splinting method of impression copings could make an influence on the accuracy of the master casts. To improve the accuracy of the master casts, splinting the squared impression copings with the additional silicone occlusion materials is recommendable.

• 한국지반공학회논문집
• /
• 제21권10호
• /
• pp.99-112
• /
• 2005

도심지에서의 지반굴착은 배면지반의 변위와 그에 따르는 건물의 손상을 유발시킨다. 굴착에 의한 지반변위의 예측과 굴착면 주변에 위치한 건물의 손상 위험도 평가는 설계단계에서 필수적인 요소이다. 본 논문에서는 기존의 굴착에 의한 지반변위 예측기법인 Peck의 방법과 Bowles의 방법을 조합하여 지보굴착에 따르는 배면지반 변위예측방법을 제안하였다. 또한, 배면지반의 Green-field 뒤틈각과 수평변형률을 이용한 인접건물 손상위험도 평가기법을 제안하였다. 이 기법은 싱가폴에서 시공중인 대규모 지반굴착공사의 설계에 성공적으로 적용되었다.

• 센서학회지
• /
• 제31권6호
• /
• pp.411-417
• /
• 2022

In this paper, we propose a sensor with a C-shaped load cell to detect force change when a person sitting on the chair in an electrical transport-convenience vehicle is pushing ground by both heels. The load cell built in the vehicle is mechanically deformed by the vertical force owing to the human weight and the horizontal force by ground-pushing feet. The deformation rate of the load cell and its distribution are simulated using finite element analysis. In the simulation, the applied loads are preset in the range of 10 kg - 100 kg with a step size of 10 kg, and the ground-pushing force by feet is increased to 40 N with a step size of 5 N with respect to each applied load level. The resistance change of the load cell was observed to be linear in simulation as well as in measurement. the maximum difference between simulation and measurement was 0.89 % when the strain gauge constant was 2.243. The constant has a large influence on the difference. The proposed sensor was fabricated by connecting an instrument amplifier and a microcontroller to a load cell and used to detect the force by ground-pushing feet. To detect foot driving, the reference signal was set to 130% of the load, and the duration of the sensor output signal exceeding the reference signal was set to 0.6 s. In a test of a vehicle built with the proposed sensor, the footpushing force by the worker could be successfully detected even when the worker was working.

• Journal of the Korean Wood Science and Technology
• /
• 제18권3호
• /
• pp.42-52
• /
• 1990

In this study, thick 24mm glulams were composed of thick. 3, 4, 6, 8mm Larch laminas to study that the theoretical analysis and the experimental analysis regarding the location of neutral axis of the glulams were compared, and to study on the effect of location of neutral axis on mechanical properties of glulam. The variation of location of neutral axis after proportional limit(or elastical limit) was studied to offer basic data to make the better composition method of glulam. The result obtained can be summarized as follows: 1. The theoretical neutral axis was 0.547 in solid wood, and also 0.547 in glulams because glulams were composed of only Larch laminas. 2. In solid wood, the deviation of the theoretical and the experimental neutral axis location was 0.1%, But in glulams, the deviation from-12.2% to + 7.8% showed nonuniform pattern but no large deviation. Because laminas was only of Larch and so the mechanical properties of laminas were monotonous. 3. The neutral axis exerted no influance on the elasticity of glulam, which meaned that the maximum shear strength in the neutral axis showed no influance on elasticity limit. 4. The only minutely lower elasticities of glulam than that of solid wood were shown. This was because of influance of glue lines of glulam on the elasticlties. 5. The failure type of glulam was wholly simple tension failure and the horizontal shear failure near neutral axis was not taken place, which was that glue line was complete in bonding and the strength of the lamina was not various but uniform. 6. The ratio of tension strain($^{\varepsilon}t$) I compression strain($^{\varepsilon}c$) initially showed uniform level After the elasticity limit. the ratio was increased with the flow of time and so the tension strain was more increased than compression strain. So this proved tension lamination technique, which is that the mechanical properties of glulam could be improved, if the lamina of more superior strength would he added on the bottom side of the glulam.

• 한국강구조학회 논문집
• /
• 제14권5호통권60호
• /
• pp.613-625
• /
• 2002

본 연구는 강한 반복하중하에서 강부재가 파괴에 이르는 손상과정을 규명하고, 손상과 관련된 인자들을 이용하여 손상지수를 제시하며, 이와 관련된 해석 기법을 개발하는 것을 목적으로 한다. 제안된 손상평가 방법은 변형이 가장 심하게 집중된 단면에 있어서 국소 변형율의 이력에 초점을 맞추어 유도되었다. 해석모델은 좌굴변형의 발생 방향을 가정한 켄틸레버형 강상자형 부재이다. 파괴에 이르는 중심 압축하중과 일정 압축하중이 가해진 상태에서 반복 제어변위가 작용하는 강부재에 대해 비선형해석을 실시하였다. 본 해석에 적용된 주요 변수는 하중 재하패턴, 강종이다. 각 변수가 파괴모드, 변형능력, 그리고 손상과정에 미치는 영향을 기술하였고, 각각 강종에 따른 파괴 과정을 비교하였다. 그 결과 강한 반복하중하에서의 강부재의 파괴는 국부좌굴에 의해서 결정되고 특히 파괴와 직접적으로 연관성이 있는 국소 소성변형율과 관련되어 있음을 알 수 있었다.

• 한국농공학회지
• /
• 제34권1호
• /
• pp.41-48
• /
• 1992

Various soil behaviors usually occurring in the geotechnical problems, such as, cutting and embankments, stability of slope, seepage, consolidations, shearing failures and liquefaction, should be predicted and analyzed in any way. An approach of these predictions may be followed by the development of the constitutive equations as first and subsequently solved by numerical methods. The purpose of this paper is develop the constitutive equation of sands uder monotonic or cyclic loadings. The constitutive equation which is based on elasto-plastic theory, modified anisotropic consolidated stress parameter by Sekiguchi et al and Pender's theory is derived. And the equation is included a new stress parameter, hardening function, Bauschinger's effects and Pender's theory. The model is later evaluated and confirmed the validity by the test data of Ottawa sand, Banwol sand Hongseong sand. The following conclustions may be drawn: 1. The consititutive equation which is based on elasto-plastic theory, modified anisotropic consolidated stress parpameter by Sekiguchi et al and Pender's theory is derived. The equation in included a new stress parameter, hardening function, Bauschinger's effect and Pender's theory. 2. For Ottawa sand, the result of the constitutive equation shows a better agreement than that of Oka et al. The result of axial strain agrees well with the tested data. However, the result of horizontal strain is little bit off for the cyclic loadings or large stress. It is thought that the deviation may be improved by considering Poisson's ratio and precise measurement of shear modulus. 3. Banwol sand is used for the strain and stress tests with different relative densitites and confining pressures. The predeicted result shows a good agreement with the tested data because the required material parameters were directly measurd and determined form this laboratory. 4. For Hongseong sand, the tests under same amplitude of cyclic deviatoric stress shows a similar result with the tested data in absolute strain. It shows the acute shape of turning point because the sine wave of input is used in the test but the serrated wave in prediction.

• Geomechanics and Engineering
• /
• 제34권2호
• /
• pp.115-124
• /
• 2023

By conducting three-dimensional simulation with consideration of small-strain characteristics of soil stiffness, the effects of excavation geometry and tunnel cover to diameter ratio on deformation mechanisms of an existing tunnel located either at a side of basement or directly underneath the basement were systematically studied. Field measurements were used to verify the numerical model and model parameters. For basement excavated at a side of an existing tunnel, the maximum settlement and horizontal displacement of the tunnel are always observed at the tunnel springline closer to basement and tunnel crown, respectively, regardless of basement geometry. By increasing basement length and width by five times, the maximum movements of tunnel located at the side of basement and directly underneath the basement increase by 450% and 186%, respectively. Obviously, tunnel movements are more sensitive to basement length rather than basement width. For basement excavated at a side of an existing tunnel, tunnel movements at basement centerline become stable when basement length reaches 10 H_e (i.e., final excavation depth). Moreover, tunnel heaves due to overlying basement excavation become stable when the normalized basement length (L/H_e) is larger than 8.0. As tunnel cover to diameter ratio varies from 2.5 to 3.0, the maximum heave and tensile strain of tunnel due to overlying basement excavation decrease by up to 41.0% and 44.5%, respectively. If basement length is less than 8 H_e, the assumption of plane strain condition of basement-tunnel interaction grossly overestimates tunnel movements, and ignores tensile strain of tunnel along its longitudinal direction. Thus, three-dimensional numerical analyses are required to obtain a reasonable estimation of tunnel responses due to adjacent and overlying basement excavations in clay.

• 한국강구조학회 논문집
• /
• 제20권3호
• /
• pp.445-454
• /
• 2008

철골모멘트접합부에 관한 이전의 연구에 의하면 RHS 기둥과 기존의 스캘럽을 가진 접합부실험체의 변형능력은 매우 열등했고, 보단부의 응력집중은 보에서 기둥으로 전달되는 모멘트의 전달효율에 영향을 받는다는 사실을 실험이나 해석적으로 밝혔다. 본 연구는 보강된 RBS 접합부와 용접수평스티프너를 사용한 효고현남부지진 이전에 지어진 모멘트 접합부를 내진보강하는데 주안점을 두었다. 이러한 내진보강은 시공성과 경제성을 고려하여 보의 하부플랜지에만 실시하였다. 접합부의 내진보강방법을 발견하고 이를 향상시키기 위해서 비선형유한요소해석을 통해서 다양한 변수를 바탕으로 한 파라메타 연구를 실시하였다.

• 한국안전학회지
• /
• 제13권4호
• /
• pp.248-255
• /
• 1998

The objective of this study is to conduct the prevention method using electromyography that is to resist the strain on L5/S1 disc and to measure the heart rate for the prevention of low back pain during lifting. EMG signals and heart rate were analyzed under the condition of fixed vertical factor (20∼80cm), two horizontal factors (35cm, 55cm), and two weight factors (10kg, 25kg) 2 times per minute for each lifting task. Eight healthy male workers performed nine-step stretching exercises which were intensified back power at the L5/S1 disc for six months. After completion of the two trials, the results were compared. The results of this analysis show that EMG signals have more an effect on the weight than the horizontal factors similar to those analyzed previous study, and are decreased. Therefore, those exercises presented very efficient. Also, there are not statistically significant differences on the analysis result of heart rate between weight factors.