• The Plant Pathology Journal
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• 제26권3호
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• pp.286-288
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• 2010

Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.

• Nuclear Engineering and Technology
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• 제55권4호
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• pp.1365-1381
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• 2023

In this paper, a procedure for evaluating the structural integrity of the PCSG (Printed Circuit Steam Generator) unit block is presented with a simplified FE (finite element) analysis technique by applying the homogenization method. The homogenization method converts an inhomogeneous elastic body into a homogeneous elastic body with same mechanical behaviour. This method is effective when the inhomogeneous elastic body has repetitive microstructures, and thus the method was applied to the sheet assembly among the PCSG unit block components. From the method, the homogenized equivalent elastic constants of the sheet assembly were derived. The validity of the determined material properties was verified by comparing the mechanical behaviour with the reference model. Thermo-mechanical analysis was then performed to evaluate the structural integrity of the PCSG unit block, and it was found that the contact region between the steam header and the sheet assembly is a critical point where large bending stress occurs due to the temperature difference.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 춘계학술대회 논문집
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• pp.127-130
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• 2004

In the recent years, lightweight components fabricated with aluminum alloys have been applied into building the automobiles. Among the several competing fabrication methods, hot forging is taken as the most reliable technique to produce suspension parts such as control arms. Generally, Al forging products have been used widely for the aircraft building with the extruded stock as a starting material. For the economical base, however, the cast stocks turn to be as the forging stocks recently after a continuously casting technique was developed to produce quite a uniform microstructure enough to use for the forging process. Even more, there is a tendency to omit the homogenization step before forging, which is considered to be an indispensable process for all kinds of Al alloy, In the present study, a series of compression test was carried out to find out how the cast structure and the following heat treatments influence the deformation behavior, that is, forging characteristic.

• Interaction and multiscale mechanics
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• 제2권1호
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• pp.45-68
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• 2009

An aim of the study is to develop an efficient numerical simulation technique that can handle the two-scale analysis of fluid permeation filters fabricated by the partial sintering technique of small spherical ceramics. A solid-fluid mixture homogenization method is introduced to predict the mechanical characters such as rigidity and permeability of the porous ceramic filters from the micro-scale geometry and configuration of partially-sintered particles. An extended finite element (X-FE) discretization technique based on the enriched interpolations of respective characteristic functions at fluid-solid interfaces is proposed for the non-interface-fitted mesh solution of the micro-scale analysis that needs non-slip condition at the interface between solid and fluid phases of the unit cell. The homogenization and localization performances of the proposed method are shown in a typical two-dimensional benchmark problem whose model has a hole in center. Three-dimensional applications to the body-centered cubic (BCC) and face-centered cubic (FCC) unit cell models are also shown in the paper. The 3D application is prepared toward the computer-aided optimal design of ceramic filters. The accuracy and stability of the X-FEM based method are comparable to those of the standard interface-fitted FEM, and are superior to those of the voxel type FEM that is often used in such complex micro geometry cases.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2058-2071
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• 2015

A comparative study was conducted to evaluate precision and accuracy in controlling the temperature dependence of encapsulated microbial time-temperature integrators (TTIs) developed using two different emulsification techniques. Weissela cibaria CIFP 009 cells, immobilized within 2% Na-alginate gel microbeads using homogenization (5,000, 7,000, and 10,000 rpm) and Shirasu porous glass (SPG) membrane technologies (10 μm), were applied to microbial TTIs. The prepared micobeads were characterized with respect to their size, size distribution, shape and morphology, entrapment efficiency, and bead production yield. Additionally, fermentation process parameters including growth rate were investigated. The TTI responses (changes in pH and titratable acidity (TA)) were evaluated as a function of temperature (20℃, 25℃, and 30℃). In comparison with conventional methods, SPG membrane technology was able not only to produce highly uniform, small-sized beads with the narrowest size distribution, but also the bead production yield was found to be nearly 3.0 to 4.5 times higher. However, among the TTIs produced using the homogenization technique, poor linearity (R²) in terms of TA was observed for the 5,000 and 7,000 rpm treatments. Consequently, microbeads produced by the SPG membrane and by homogenization at 10,000 rpm were selected for adjusting the temperature dependence. The E_a values of TTIs containing 0.5, 1.0, and 1.5 g microbeads, prepared by SPG membrane and conventional methods, were estimated to be 86.0, 83.5, and 76.6 kJ/mol, and 85.5, 73.5, and 62.2 kJ/mol, respectively. Therefore, microbial TTIs developed using SPG membrane technology are much more efficient in controlling temperature dependence.

• Advances in nano research
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• 제7권6호
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• pp.379-390
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• 2019

Putting emphasis on the effect of existence of porosity in the functionally graded materials (FGMs) on the dynamic responses of waves scattered in FG nanobeams resulted in implementation of a novel porosity-based homogenization method for FGMs and show its applicability in a wave propagation problem in the presence of axial pre-load for the first time. In the employed porosity-dependent method, the coupling between density and Young's moduli is included to consider for the effective moduli of the FG nanobeam by the means of a more reliable homogenization technique. The beam-type element will be modeled via the classical theory of beams, namely Euler-Bernoulli beam theory. Also, the dynamic form of the principle of virtual work will be extended for such nanobeams to derive the motion equations. Applying the nonlocal constitutive equations of Eringen on the obtained motion equations will be resulted in derivation of the nanobeam's governing equations. Depicted results reveal that the dispersion responses of FG nanobeams will be decreased as the porosity volume fraction is increased which must be noticed by the designers of advanced nanosize devices who are interested in employment of wave dispersion approach in continuous systems for specific goals.

• 터널과지하공간
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• 제19권2호
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• pp.158-166
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• 2009

Hoek-Brown 암석에서 응력에 의해 발생되는 균열의 전파특성을 수치해석적으로 분석하기 위한 기초연구로서 탄성균열의 균질화 기법이 제안되었다. 균열의 개시조건으로 Hoek-Brown 경험식이 이용되었고, 균열의 방향 탐색을 위해 임계면법이 이용되었다. 균열물질과 무결암으로 구성된 대표체적에 대해 체적평균 응력 및 변형률 개념을 적용하여 균열과 신선암의 역학적 특성을 균질화시킴으로써 등가 이방성매질의 구성관계식을 유도하였다. 제안된 균질화모델을 포트란 코드로 작성하여 상업유한요소 코드인 COSMOSM에 이식하였다. 제안된 수치해석모델의 적합성을 검증하기 위하여 2차원 평면변형률조건에서 수치 일축압축시험을 실시하였다. 모델 상하부 가압면의 구속조건을 달리한 2가지 해석모델을 선정하여 구속조건이 일축압축시험편의 변형 및 파단면 형성형태에 미치는 영향이 분석되었다. 균열의 균질화를 고려한 수치 일축압축시험 결과는 실제 실험에서 발생되는 형태와 유사한 변형거동 및 파단면 형성 특성을 잘 나타내었다.

• 대기
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• 제31권5호
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• pp.553-562
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• 2021

The long-term trend of surface wind speed in Korea is estimated by correcting wind measurements at 29 KMA weather stations from 1985 to 2019 with physical and statistical homogenization. The anemometer height changes at each station are first adjusted by applying physical homogenization using the power-law wind profile. The statistical homogenization is then applied to the adjusted data. A standard normal homogeneity test (SNHT) is particularly utilized. Approximately 40% of inhomogeneities detected by the SNHT match with the sea-level-height change of each station, indicating that an SNHT is an effective technique for reconciling data inhomogeneity. The long-term trends are compared with homogenized data. Statistically significant negative trends are observed along the coast, while insignificant trends are dominant inland. The mean trend, averaged over all stations, is -0.03 ± 0.07 m s^-1 decade^-1. This insignificant trend is due to a trend change across 2001. A decreasing trend of -0.10 m s^-1 decade^-1 reverses to an increasing trend of 0.03 m s^-1 decade^-1 from 2001. This trend change is consistent with mid-latitude wind change in the Northern hemisphere, indicating that the long-term trend of surface wind speed in Korea is partly determined by large-scale atmospheric circulation.

• Interaction and multiscale mechanics
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• 제4권2호
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• pp.107-122
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• 2011

Mechanical behavior in nano-sized structures differs from those in macro sized structures due to surface effect. As the ratio of surface to volume increases, surface effect is not negligible and causes size-dependent mechanical behavior. In order to identify this size effect, atomistic simulations are required; however, it has many limitations because too much computational resource and time are needed. To overcome the restrictions of the atomistic simulations and graft the well-established continuum theories, the continuum model considering surface effect, which is based on the bridging technique between atomistic and continuum simulations, is introduced. Because it reflects the size effect, it is possible to carry out a variety of analysis which is intractable in the atomistic simulations. As a part of the application examples, the homogenization method is applied to micro/nano thin films with porosity and the homogenized elastic coefficients of the nano scale thickness porous films are computed in this paper.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.1390-1395
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• 2005

To analyze the effects of oyster shell particles, inserted in the self-hardening matrix such as cement paste, on strength, homogenization analysis using micro structure was used to estimate and assess the apparent elastic modulus of oyster shell particle. DIB modeling technique was used to represent of the micro structure of oyster shell mixed concrete. The results showed that the apparent elastic modulus of LOS (large oyster shell particle) was changed with the amount of LOS inserted. In particular, when the amount of LOS was 200% of the weight of cement, the apparent elastic modulus of LOS tended to decrease rapidly. This could mean that the strength of oyster shell mixed concrete is much affected by LOS inserted material in mixed ratio of 200%.