• Title/Summary/Keyword: Holding methods

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Dietary supplementation of Eucommia leaf extract to growing-finishing pigs alters muscle metabolism and improves meat quality

  • Zhenglei Shen;Chuxin Liu;Chuangye Deng;Qiuping Guo;Fengna Li;Qingwu W. Shen
    • Animal Bioscience
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    • v.37 no.4
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    • pp.697-708
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    • 2024
  • Objective: The objective of this study was to investigate the influence of dietary supplementation of Eucommia ulmoides leaf extract (ELE) on muscle metabolism and meat quality of pigs with and without pre-slaughter transportation. Methods: In a 43-day feeding experiment, a total of 160 pigs with an initial body weight 60.00±2.00 kg were randomly assigned into four groups in a completely randomized design with 10 replicates. Pigs in groups A and C were fed a basal diet and pigs in groups B and D were fed a basal diet supplemented with 0.5% ELE. Pigs were slaughtered with (group B and D) or without (group A and C) pre-slaughter transport. Muscle chemical composition, postmortem glycolysis, meat quality and muscle metabolome were analyzed. Results: Dietary ELE supplementation had no effect on the proximate composition of porcine muscle, but increased free phenylalanine, proline, citruline, norvaline, and the total free amino acids in muscle. In addition, dietary ELE increased decanoic acid and eicosapentaenoic acid, but decreased heptadecanoic acid, oleic acid, trans-oleic acid, and monounsaturated fatty acids in muscle. Meat quality measurement demonstrated that ELE improved meat water holding capacity and eliminated the negative effects of pre-slaughter transport on meat cooking yield and tenderness. Dietary ELE reduced muscle glycolytic potential, inhibited glycolysis and muscle pH decline in the postmortem conversion of muscle to meat and increased the activity of citrate synthase in muscle. Metabolomics analysis by liquid chromatographic tandem mass spectrometric showed that ELE enhanced muscle energy level, regulated AMP-activated protein kinase (AMPK) signaling, modulated glycogenolysis/glycolysis, and altered the metabolism of carbohydrate, fatty acids, ketone bodies, amino acids, purine, and pyrimidine. Conclusion: Dietary ELE improved meat quality and alleviated the negative effect of pre-slaughter transport on meat quality by enhancing muscle oxidative metabolism capacity and inhibiting glycolysis in postmortem muscle, which is probably involved its regulation of AMPK.

COMPARISON OF THE TIME-SIGNAL SYSTEM OF AUTOMATIC WATER CLOCKS DURING THE YUAN DYNASTY AND THE KING SEJONG ERA OF THE JOSEON DYNASTY (원대(元代)와 세종대(世宗代) 자동 물시계 시보시스템 비교)

  • YONG-HYUN YUN;SANG HYUK KIM;BYEONG-HEE MIHN;BYONG GUEN LEEM
    • Publications of The Korean Astronomical Society
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    • v.39 no.1
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    • pp.1-12
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    • 2024
  • In this study, we investigated the time signal devices of Deungnu (circa 1270) and Gungnu (1354), the water clocks produced during the Yuan Dynasty (1271-1368). These clocks influenced Heumgyeonggaknu (1438) of the Joseon Dynasty (1392-1910), exemplifying the automatic water clocks of the Yuan Dynasty. Deungnu, Gungnu, and Heumgyeonggaknu can be considered as automatic mechanical clocks capable of performances. The Jega-Yeoksang-Jip (Collection of Calendrical and Astronomical Theories of Various Chinese Masters) contains records of Deungnu extracted from the History of the Yuan Dynasty. We interpreted these records and analyzed reproduction models and technical data previously produced in China. The time signal device of Deungnu featured a four-story structure, with the top floor displaying the four divine constellations, the third floor showcasing models of these divinities, the second floor holding 12-h jacks and a 100-Mark ring, and the first floor with four musicians and a 100-Mark Time-Signal Puppet providing a variety of visual attractions. We developed a 3D model of Deungnu, proposing two possible mechanical devices to ensure that the Time-Signal Puppet simultaneously pointed to the 100-Mark graduations in the east, west, south, and north windows: one model reduced the rotation ratio of the 100-Mark ring to 1/4, whereas the other model maintained the rotation ratio using four separate 100-Mark rings. The power system of Deungnu was influenced by Suunuisangdae (the water-driven astronomical clock tower) of the Northern Song Dynasty (960-1127); this method was also applied to Heumgyeonggaknu in the Joseon Dynasty. In conclusion, these automatic water clocks of East Asia from the 13th to 15th centuries symbolized creativity and excellence, representing scientific devices that were the epitome of clock-making technology in their times.

Comparison of Foot Pressure Distribution During Single-leg Squat in Individuals With and Without Pronated Foot

  • Il-kyu Ahn;Gyeong-tae Gwak;Ui-jae Hwang;Hwa-ik Yoo;Oh-yun Kwon
    • Physical Therapy Korea
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    • v.31 no.1
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    • pp.40-47
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    • 2024
  • Background: Single-leg squat (SLS)s are commonly used as assessment tool and closed kinetic exercises are useful for assessing performance of the lower extremities. Pronated feet are associated with foot pressure distribution (FPD) during daily activities. Objects: To compare the FPD during SLSs between groups with pronated and normal feet. Methods: This cross-sectional study included 30 participants (15 each in the pronated foot and control groups) are recruited in this study. The foot posture index was used to distinguish between the pronated foot and control groups. The Zebris FDM (Zebris Medical GmbH) stance analysis system was used to measure the FPD on the dominant side during a SLS, which was divided into three phases. A two-way mixed-model ANOVA was used to identify significant differences in FPD between and within the two groups. Results: In the hallux, the results of the two-way mixed-model ANOVAs revealed a significant difference between the group and across different phases (p < 0.05). The hallux, and central forefoot were significantly different between the group (p < 0.05). Moreover, significant differences across different phases were observed in the hallux, medial forefoot, central forefoot, lateral forefoot, and rearfoot (p < 0.05). The post hoc t-tests were conducted for the hallux and forefoot central regions. In participants with pronated foot, the mean pressure was significantly greater in hallux and significantly lower, in the central forefoot during the descent and holding phases. Conclusion: SLSs are widely used as screening tests and exercises. These findings suggest that individuals with pronated feet should be cautious to avoid excessive pressure on the hallux during the descent-to-hold phase of a SLS.

Studies on Cryotop Vitrification Method for Simple Freezing of Hanwoo Embryos (한우 수정란의 간이 동결을 위한 유리화 동결법에 관한 연구)

  • Lee, Hae-Lee;Kim, Sang-Hun;Kim, Yong-Jun
    • Journal of Embryo Transfer
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    • v.29 no.1
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    • pp.13-19
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    • 2014
  • This study was carried out to study the survival rate of thawed Hanwoo embryos frozen by the slow-rate freezing or the cryotop vitrification method. Hanwoo cumulus-oocyte complexes were recovered from ovaries at a slaughter house, matured for 20~22 hours, fertilized with Hanwoo semen for 5~6 hours, and cultured for 7~9 days in $38.5^{\circ}C$, 5% $CO_2$ incubator. For freezing, Day 7~9 blastocysts were collected. Embryos for the slow-rate freezing were equilibrated in 1.8 M ethylene glycol (EG) with Dulbecco's phosphate-buffered saline (D-PBS). Programmable cell freezer was precooled down to $-7^{\circ}C$, and the straw was seeded during 8 minutes-holding time, and was cooled to $-35^{\circ}C$ at the cooling rate of $0.3^{\circ}C/min$, and then was plunged and stored in liquid nitrogen. Embryos for the cryotop vitrification were treated in TCM199 with 0.5 M sucrose, 16% EG, 16% dimethylsulfoxide (DMSO). Embryos were then loaded individually onto cryotop and plunged directly into liquid nitrogen. The survival rates of embryos frozen by these two freezing methods were evaluated at 12 to 24h post-thawing. The survival rates of frozen/thawed Hanwoo embryos by the cryotop vitrification method ($56.86{\pm}26.53%$) were slightly higher than those by the slow-rate freezing method ($55.07{\pm}26.43%$) with no significant difference. Using the cryotop vitrification and the slow-rate freezing of Hanwoo blastocysts on Day 7 following in-vitro fertilization (IVF) treatment, the survival rates of frozen/thawed Hanwoo embryos were $72.65{\pm}18.3%$ and $79.06{\pm}17.8%$, respectively. The survival rates by the cryotop vitrification were higher than those by the slow-rate freezing on both Day 8 and 9 with significantly higher survival rate on Day 9 (p<0.05). Using the cryotop vitrification and the slow-rate freezing of Hanwoo embryos to compare between three different blastocyst stages, the survival rates of the blastocyst stage embryos were $66.22{\pm}18.8%$ and $45.76{\pm}12.8%$, respectively with higher survival rate by the vitrification method (p<0.05). And the survival rate of expanded blastocysts was higher than those of early blastocysts and blastocysts in two freezing methods with significantly higher survival rate by the slow-rate freezing method (p<0.05).

Economic Evaluation of Rare Earth Elements Contained in Coal Ash (석탄재에 포함된 희토류의 경제성 평가)

  • Kim, Youngjin;Kim, Seunghyun;Lee, Jaeryeong
    • Resources Recycling
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    • v.28 no.6
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    • pp.26-35
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    • 2019
  • This study aims to introduce and economical review on the possibilities of rare earth elements(REEs) recovery from coal ashes and the analysis of economical evaluation factors based on the data for securing domestic rare earth elements. The cut-off grade of REEs on recovering from coal ash was confirmed to be 1,000 ppm on total rare earth oxides(TREO) basis, and while the economic value of coal ash changed with contents and specific elements of rare earth elements. This shall be resulted in the price differences of rare earth elements required by the current industry, and it probably varies depending on the future demand of rare earth components. For developing of commercial recovery technology on REEs in coal ashes, many researches have been carried out by various analyzing methods, such as evaluation of holding value of REEs in ashes, assessment between supply and demand of industry, comparison of investment and its profitability for the REEs's production from coal ashes, and so on. Although these methods have been suggested, its recovery system with economical feasibility could not been confirmed up to present. In this reason, the process design of recovering REEs from coal ash shall be researched continuously to solve the problems of the global rare earth market. And also these researches shall be conducted actively in Korea for the purpose of securing the REEs resources and their recovering technologies.

Conservation of Buddhist Monk Samyeong's Geumran-gasa and Jangsam, the National Folklore Cultural Heritage No.29 (국가민속문화재 제29호 사명대사의 금란가사와 장삼의 과학적 조사와 보존)

  • Chung, Young Ran;An, Bo Yeon
    • Journal of Conservation Science
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    • v.33 no.6
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    • pp.443-456
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    • 2017
  • The Geumran-gasa (Kasaya) and Jangsam of the monk Samyeong are historical relics and were designated as object No.29, a Buddhist costume of the Joseon Dynasty, by the National Folklore Cultural Heritage, in 1973. In 1984, after being treated for the purposes of washing and preserving the shape of the garments, the object was kept in the Temple Museum of Pyochungsa, as their holding institution. However, regular inspection conducted by the Cultural Heritage Administration in 2013 determined the need for a re-treatment. The Geumran-gasa had been originally made with 25 strips, but it had been separated into two parts and many pieces were lost. A part of the collar in the Jangsam was lost as well. Therefore, both relics needed to be restored. The re-treatment process involved pre-investigation, washing, reinforcement fabric dyeing, restoration, and fumigation. In particular, we focused on reinforcing the damaged parts and restoring the missing parts by applying advanced materials and methods in order to restore the original form as much as possible. This conservation is the result of the re-treatment of the cultural properties by replacing the old material used in past treatments and applying advanced methods under the basic principle of reversibility in conservation treatment.

Artificial Control of ZnO Nanorods via Manipulation of ZnO Nanoparticle Seeds (산화아연 나노핵의 조작을 통한 산화아연 나노로드의 제어)

  • Shin, Kyung-Sik;Lee, Sam-Dong;Kim, Sang-Woo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.399-399
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    • 2008
  • Synthesis and characterization of ZnO structure such as nanowires, nanorods, nanotube, nanowall, etc. have been studied to multifunctional application such as optical, nanoscale electronic and chemical devices because it has a room-temperature wide band gap of 3.37eV, large exiton binding energy(60meV) and various properties. Various synthesis methods including chemical vapor deposition (CVD), physical vapor deposition, electrochemical deposition, micro-emulsion, and hydrothermal approach have been reported to fabricate various kinds of ZnO nanostructures. But some of these synthesis methods are expensive and difficult of mass production. Wet chemical method has several advantage such as simple process, mass production, low temperature process, and low cost. In the present work, ZnO nanorods are deposited on ITO/glass substrate by simple wet chemical method. The process is perfomed by two steps. One-step is deposition of ZnO seeds and two-step is growth of ZnO nanorods on substrates. In order to form ZnO seeds on substrates, mixture solution of Zn acetate and Methanol was prepared.(one-step) Seed layers were deposited for control of morpholgy of ZnO seed layers by spin coating process because ZnO seeds is deposited uniformly by centrifugal force of spin coating. The seed-deposited samples were pre-annealed for 30min at $180^{\circ}C$ to enhance adhesion and crystallinnity of ZnO seed layer on substrate. Vertically well-aligned ZnO nanorods were grown by the "dipping-and-holding" process of the substrates into the mixture solution consisting of the mixture solution of DI water, Zinc nitrate and hexamethylenetetramine for 4 hours at $90^{\circ}C$.(two-step) It was found that density and morphology of ZnO nanorods were controlled by manipulation of ZnO seeds through rpm of spin coating. The morphology, crystallinity, optical properties of the grown ZnO nanostructures were carried out by field-emission scanning electron microscopy, high-resolution electron microscopy, photoluminescence, respectively. We are convinced that this method is complementing problems of main techniques of existing reports.

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Effects of handling methods of seed tuber pieces at planting on sprouting of fall grown potatoes (파종당시의 종서취급방식이 추작 마령서의 발아에 미치는 영향)

  • Bong-Ku Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.8 no.1
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    • pp.91-97
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    • 1970
  • It is one of the important subjects in fall crop production of potatoes in korea to find the proper direct planting method as a substitute for transplanting method by sprouting inducement at seed-bed. From this point the author has undertaken this experiments to examine the effects of several handling methods of seed tuber pieces at planting on sprouting of fall grown potatoes at Ansung Agricultural College from August to september in 1969. The results of experiments induced sprouting with Gibberellin treatment using the variety Irish Cobbler were as follows; 1. Sun light exposure treatment to cut surface of potato seed pieces resulted in rotting of seed pieces and delay of sprouting and bud growth. Exposure more than three hours induced severe rotting(more than fifty percent) of seed pieces (Table I). 2. When soil moisture are abundant and the duration of sun light. exposure is short, even direct planting method of treated seed pieces with Gibberellin resulted in good potato stands (Table 2). 3. The direct planting method, as the rotting of seed pieces are avoidable are applicable in fall crop production of potatoes us a substitute for transplanting method because the sprouting and bud growth are promoted more in case of direct planting than transplanting (Table 3). 4. When the humidity in the bottle was low no healing effects was found by holding the seed pieces in large closed bottle under the trees for three days before planting (Table 2). 5. If duration of sunlight exposure is short, no difference of potato stands between clear day planting and cloudy day plant ing (Table 3). 6. As long as soil moisture deficiency. was not induced the irrigation at planting shows no effects to protect the seed piece rotting after planting (Table 3).

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The Review of a Standardized Method in Retention-Enema: a Clinical Study (보류관장의 표준화된 방법을 위한 고찰: 임상논문을 중심으로)

  • Song, Eun-Young;Lee, Eui-Ju;Bu, Yong-Min;Shin, Seung-Won;Chai, Kwang-Min;Yoo, Jung-Hee;Kim, Hyo-Jin;Baek, Jong-Heon;Lee, Jun-Hee;Koh, Byung-Hee
    • Journal of Sasang Constitutional Medicine
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    • v.25 no.2
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    • pp.108-114
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    • 2013
  • Objectives This study was aimed to review a standardized method of retention enema by extracting elements associated with methods of retention enema from papers involved. Methods 1) Data sources : Retrieval was made, using 'Enema' or 'Retention Enema' as search words in MEDLINE, The Cochrane Library, KISS, RISS, and NDSL DB. 2) Study selection : Two authors excluded irrelevant papers and chose qualified abstracts in the rest of the papers. The chosen studies were reviewed fully by the authors so that they could screen the significant papers based on the exclusion criteria. 3) Data extraction : Data on the total number of subjects, the target disease, the type, quality, length, diameter of catheter, the insertion length of catheter, the sample, the sample volume, the process of retention enema and the number of performing retention enema were extracted from the selected studies. Results & Conclusions The retention enema can be applied to gastrointestinal diseases, genitourinary diseases, metabolic diseases such as hypertension and diabetes, headaches and neurological disorders. The standardized treatment method is as following: 1) inserting a rental tube of 18-28 Fr that measures 20 to 30 cm into the patient's anus, 2) injecting herbal medicines which have purgative action or benefit on stomach and intestinal tract at the rate of 4 ml/min, and 3) holding them for 10 to 15 minutes.

Effects of Brine Immersion and Electrode Contact Type Low Voltage Ohmic Thawing on the Physico-chemical Properties of Pork Meat (염수 침지식 및 전극 접촉식 저전압 Ohmic 해동 처리가 돈육의 이화학적 특성에 미치는 효과)

  • Hong, Geun-Pyo;Min, Sang-Gi;Ko, Se-Hee;Shim, Kook-Bo;Seo, Eun-Ju;Choi, Mi-Jung
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.416-423
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    • 2007
  • This study investigated the effect of ohmic thawing on the physicochemical properties of pork meat. The physicochemical properties of pork meat thawed by brine immersion and electrode contact ohmic systems were compared. A more rapid thawing rate was seen with the electrode contact thawing system than with brine immersion. No significant differences in pH were found with increasing voltage for both thawing methods (p>0.05). Increasing the voltage level tended to decrease drip loss, resulting in increased water holding capacity. The shear forces of pork thawed at 50 V did not differ from the control (p>0.05) for both thawing methods. Although significantly high TBARS (p<0.05) values were found at 20 and 40 V for immersion, and 0 V for contact thawing, increasing the voltage level tended to decrease the TBARS values. Regarding TVBN, no significant effect was observed with increasing voltage levels (p>0.05). The total color difference of pork was significantly higher (p<0.05) with immersion thawing than with electrode contact thawing. These results indicate that brine immersion thawing is favorable at high voltage levels, while lower voltage levels are applicable for electrode contact thawing.