• Title/Summary/Keyword: High-isolation

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Isolation of High Quality RNA from Seeds of the Mungbean (Vigna radiata) (녹두 종자의 RNA 분리 방법)

  • Kim, Kyeong-Im;Ku, Ja-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.274-276
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    • 2006
  • Mungbean (Vigna radiata) is a legume to East Asia that has great potential for development as traditional food and industrial crop. It produces both protein and starch rich grain. The low variability of the existing gene pool of the mungbean limits the use of conventional plant breeding to address this problem. For this purpose, an efficient means of RNA isolation from mungbean seed tissues was developed. The quality of RNA obtained by this method was sufficient for use in RT-PCR and RNA analysis.

Evaluation of high performance isolation spring mount by attached masses (부가질량 효과에 따른 고효율 방진마운트의 진동저감 성능평가)

  • Ho, Kyoung-Chan;Lee, Byoung-Chul;Lee, Yong-Hyun;Lee, Young-Je;Kim, Min-Bae
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.11a
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    • pp.1247-1252
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    • 2007
  • The machine equipment of the building inside occurs the vibration. In order to reduce this vibration spring, rubber pad is used. But efficiency of this classical mount is restricted at specific frequency. Also maximum efficiency design is complicated. This paper proposes and examines a attached mass isolation system that is used to reduce transmitted vibrations from machines onto their floor support. With attached mass the low frequency performance is improved overall. The performance is showed in 2 degree of freedom model test. And the proposed isolator has been validated by dynamic test and good agreement between theoretical and experimental results has been obtained.

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Signal Analysis of Motor Current for End Point Detection in the Chemical Mechanical Polishing of Shallow Trench Isolation with Reverse Moat Structure

  • Park, Chang-Jun;Kim, Sang-Yong;Seo, Yong-Jin
    • KIEE International Transactions on Electrophysics and Applications
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    • v.2C no.5
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    • pp.262-267
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    • 2002
  • In this paper, we first studied the factors affecting the motor current (MC) signal, which was strongly affected by the systematic hardware noises depending on polishing such as pad conditioning and arm oscillation of platen and recipe, head motor. Next, we studied the end point detection (EPD) for the chemical mechanical polishing (CMP) process of shallow trench isolation (STI) with reverse moat structure. The MC signal showed a high amplitude peak in the fore part caused by the reverse meal. pattern. We also found that the EP could not be detected properly and reproducibly due to the pad conditioning effect, especially when conventional low selectivity slurry was used. Even when there was no pad conditioning effect, the EPD method could not be applied, since the measured end points were always the same due to the characteristics of the reverse moat structure with an open nitride layer.

A Study on Base Isolation Performance of MR Dampers Using Clipped-Optimal Control (Clipped-Optimal Control을 사용한 MR 감쇠기의 면진성능에 관한 연구)

  • 고봉준;이종세
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2003.04a
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    • pp.529-536
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    • 2003
  • As large structures such as high-rise buildings and cable-stayed bridges become lighter and more flexible, the necessity of structural control for reducing excessive displacement and acceleration due to seismic excitation is increased. As a method to minimize seismic damages, various base isolation systems are adopted or considered for adoption. In this study, the seismic performance of MR dampers are studied and compared with that of the NZ system as a base isolation system. As the control algorithm of the MR damper, the clipped-optimal control(applied LQR method) is employed. A five-story building is modeled and the seismic performance of the two systems subjected to three different earthquakes is compared. The results show that the MR damper system can provide superior protection than the NZ system for a wide range of ground motions.

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Finite Element Analysis and Design of a Lead-Rubber-Bearing System for Seismic Isolation (면진 LRB(Lead Rubber Bearing) 시스템의 유한요소 해석 및 설계)

  • 송우진
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1999.03b
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    • pp.229-232
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    • 1999
  • The seismic isolation technology has appeared to be increasingly necessary for highway brides LNG tank nuclear power plant and building structures in view of recent frequent earthquake vibrations in Korea. Also high-technology industries required effective seismic protection. The LRB(Lead Rubber Bearing) systen has been counted as the most effective way for seismic isolation which is now under development and widely used in industries. Hear the commercial FEM software for nonlinear analysis MARC has provided force-displacement curves on the rubber system. The analysis has been carried out about four cases ; 29.5mm and 59mm horizontal dislacements with/without a center hole. The unknown constants of the strain energy function of Ogden model have been obtained by a tension test,

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The Method for detecting leakage current of a electric vehicle (전기 구동 차량의 누설 전류 검출 기법)

  • Park, Hyunseok;Eom, Jeongyong
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.139.1-139.1
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    • 2011
  • Electric vehicle use independent electricity of high voltage. if isolation of electricity is destructed, devices and people are considerably damaged. Therefore, detection of ground fault is necessary for electric vehicle. As the existing detection method of ground fault can not detect ground fault when isolation of both positive side and negative side of electricity is destructed, and change of voltage of electricity. This paper proposed detection method for ground fault of both two sides of electricity and change of voltage. The proposed method is verified by analysis of equivalent circuit.

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Isolation and Characterization of Novel Alginate-Degrading Pseudoalteromonas sp. Y-4

  • Cho, Hyeon-Ah;Kim, Hyun-Woo;Kim, Young-Mog
    • Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.259-263
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    • 2012
  • To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoalteromonas sp., based on morphological, biochemical, 16S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalteromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginate-degrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at $30^{\circ}C$. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.

Seismic Isolation Systems Incorporating with RC Core Walls and Precast Concrete Perimeter Frames -Shimizu Corporation Tokyo Headquarter-

  • Shimazaki, Dai;Nakagawa, Kentaro
    • International Journal of High-Rise Buildings
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    • v.4 no.3
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    • pp.181-189
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    • 2015
  • Shimizu Corporation Tokyo Headquarters, one of the city's leading office buildings, features many pioneering technologies that contribute to a sustainable society through environmental stewardship and a sophisticated disaster management facility. In terms of structural engineering, a seismic isolation system incorporating reinforced concrete core walls and precast concrete perimeter frames create a robust structure in the event of a large earthquake. In addition to the seismic resistance of the structure, several pioneering construction methods and materials are adopted. This office building can serve as a basis for new design and construction approaches and methodologies to ensure safe and economical structures.

Isolation and Characterization of Chloroplast DNA in Korea Ginseng, Panax gindeng C.A. Meyer (고려인삼(Panax ginseng C.A. Meyer)의 엽록체 DNA 분리 및 특성조사)

  • Lee, Jeong-Heon;Lim, Yong-Pyo;Choi, Kwang-Tae
    • Journal of Ginseng Research
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    • v.17 no.1
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    • pp.39-44
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    • 1993
  • In Korean ginseng, Panax ginseng C.A Meyer, it was difficult to isolate chloroplast DNA with classical methods, because of the high polysaccharide content of ginseng chloroplast The simple and efficient method of chloroplast DNA isolation from ginseng leaves has been developed by motificalion of recently advanced methods. Also, it can be successfully applied to ctDNA isolation of Chinese cabbage, radish, petunia tobacco as well as ginseng. Isolated chloroplast DNA from ginseng was digested with various restriction endonucleases. It was estimated that the molecular weight of Korean ginseng chloroplast DNA was about 142 kb. There was no difference in restriction endonuclease digestion patterns between two variants of Korean ginseng, which are Jakyung-Jong (violet-stem variant) and Hwang- sook-Jong (yellow-berry variant).

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Design of the Open-Loop Combined Meandered-Line 1-Layer Radiator for Diversity Antennas with Size-Reduction and Improved Isolation (다이버시티 안테나의 소형화와 격리도 향상을 위한 미앤더 선로와 개방형 루프가 결합된 방사구조의 설계)

  • Mok, Se-Gyoon;Kahng, Sung-Tek;Kim, Yong-Jin
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.1
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    • pp.110-116
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    • 2012
  • This paper proposes a new diversity antenna which is the base of MIMO, tunable and reconfigurable antennas. The antenna has a small size and high inter-antenna isolation resulting from the compact radiating element comprising a meandered line and an open-loop combined in one limited uniplanar space and a modified T-shaped decoupling structure, respectively. In a WiMAX band, the radiating element and the entire antenna are $0.092{\lambda}$ and $0.2216{\lambda}$ in size, which shows effective size-reduction and the gain and efficiency of the proposed antenna attached to the ground of a handheld device are 3.7dBi and 56% acceptable to the industrial standard.