• Nutrition Research and Practice
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• 제14권5호
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• pp.425-437
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• 2020

BACKGROUND/OBJECTIVES: Different fatty acids exert different health benefits. This study investigated the potential protective effects of perilla, olive, and safflower oils on high-fat diet-induced obesity and colon inflammation. MATERIALS/METHODS: Five-week old, C57BL/6J mice were assigned to 5 groups: low-fat diet (LFD), high-fat diet (HFD) and high-fat diet supplemented with-perilla oil (HPO), olive oil (HOO), and safflower oil (HSO). After 16 weeks of the experimental period, the mice were sacrificed, and blood and tissues were collected. The serum was analyzed for obesity- and inflammation-related biomarkers. Gene expression of the biomarkers in the liver, adipose tissue, and colon tissue was analyzed. Micro-computed tomography (CT) analysis was performed one week before sacrifice. RESULTS: Treatment with all the three oils significantly improved obesity-induced increases in body weight, liver weight, and epididymal fat weight as well as serum triglyceride and leptin levels. Treatment with perilla oil (PO) and safflower oil (SO) increased adiponectin levels. The micro-CT analysis revealed that PO and SO reduced abdominal fat volume considerably. The mRNA expression of lipogenic genes was reduced in all the three oilsupplemented groups and PO upregulated lipid oxidation in the liver. Supplementation of oils improved macroscopic score, increased colon length, and decreased serum endotoxin and proinflammatory cytokine levels in the colon. The abundance of Bifidobacteria was increased and that of Enterobacteriaceae was reduced in the PO-supplemented group. All three oils reduced proinflammatory cytokine levels, as indicated by the mRNA expression. In addition, PO increased the expression of tight junction proteins. CONCLUSIONS: Taken together, our data indicate that the three oils exert similar anti-obesity effects. Interestingly, compared with olive oil and SO, PO provides better protection against high-fat diet-induced colon inflammation, suggesting that PO consumption helps manage inflammation-related diseases and provides omega-3 fatty acids needed by the body.

• 동아시아식생활학회지
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• 제9권4호
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• pp.427-434
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• 1999

This study was conducted to investigate the effects of vitamin I supplementation on renal function in high fat diet and adriamycin (ADR) induced experimental nephrotic syndrome in model rats. The effects of vitamin E supplementation on renal function in high fat diet and ADR treated rats were as follows. Kidney weight was decreased by vitamin E supplementation. Serum total protein was increased by the excess supplementation. Blood urea nitrogen(BUN) was decreased by the high supplementation. However, serum albumin and creatinine showed no significant differences between groups. Urinary volume tended to increase by vitamin I supplementation. Urinary urea-N tended by vitamin I supplementation. Particularly glomerular filtration rate(GFR) was significantly decreased by vitamin E supplementation. These results suggested that vitamin E supplementation could alleviate the adverse effects caused in renal function by highfatdiet and ADR treatments.

• 대한예방한의학회지
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• 제13권1호
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• pp.41-58
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• 2009

Objectives: The purpose of this study was to investigate the effects of Hyolbuchukeo-tang extracts on the hyperlipidemia rats induced by high fat diet. Methods and Materials: In vitro; The extracts prepared for Hyolbuchukeo-tang by hot water extraction (HH), fermentation(HF) and UMPM extraction(HU) method. The extracts were examined for levels of polyphenol compounds, antioxidant activities, and inhibitory potencies for HMG-CoA reductase. In vivo; Sprague-Dawley male rats of weighing $150{\pm}5g$ were randomly divided into six groups ; normal control diet(NC), and high fat diet(HC), high fat diet with treated lovastatin of 10mg/kg(PC), high fat diet with Hyolbuchukeo-tang extracts; HH, HF and HU treated extracts of 300mg/kg, respectively. Also, we compared the effects of the extracts of HH, HF and HU on rats fed high fat diet for four weeks. Results: 1. The content of polyphenol compounds and electron donating abilities(EDAs) was the HF higher than HH and HU. The superoxide dismutase(SOD)-like activities were proportionate in consistency and they appeared highly from all extracts. The HMG-CoA reductase inhibition activities was highest activities in the HU. 2. The activities of serum GOT and GPT were significantly lower in the HH and HF groups. The level of serum triglyceride was significantly decreased in the HF group. HH and HU groups were significantly decreased in the atherogenic index(AI). The total cholesterol concentration in liver was significantly decreased in the HF group, and HU showed more significantly decreased in the triglyceride than of the lovastatin. Also, photomicrographs of liver tissue showed higher fat accumulation in the HC group than in the HH, HF and HU groups. Conclusions: These result suggest that the hyper-lipidemia caused by a high fat diet was effectively inhibited the administration of HF and HU.

• Journal of Nutrition and Health
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• 제25권2호
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• pp.109-115
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• 1992

This study was undertaken to determine the effect of dietary fat to carbohydrate ratio on plasma glucose. free fatty acid level and hepatic glucokinase activity in normal or insulin treated diabetic rats. Sprague-Dawley male rats were fed with 3 different but isocaloric diets for 5 weeks. Diet 1 made to have low fat(4% corn oil and 65.8% corn starch wt/wt) : diet 2 medium fat (12% : 47.8%) : diet 3 high fat (20% : 29.8%) In the normal rats an apparent increase of GK activity was observed from the animal fed low fat diet when compared with other groups. GK activities were decreased in all the alloxan-diabetic rats than the normal rats. When insulin was injected the GK activities in all the livers of alloxan-diabetic rats restored to normal level and GK activity was highest in low fat group. In the entire group significant relationships were seen between the plasma glucose and GK activities(r=-0.6, p<0.001) FFA levels and GK activities(r=-0.63 p<0.001) Both in normal and insulin treated diabetic rats significantly depressed level of hepatic GK activity was observed in the livers of animals fed high fat diet for 5 weeks and depressed level of GK activity may be related to insulin resistance.

• Journal of Nutrition and Health
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• 제22권2호
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• pp.63-69
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• 1989

정상백서를 부신절제한 후 각각의 부신호르몬을 투여하여, 고탄수화물, 고지방, 고단백질식이가 부신절제 백서의 대사에 미치는 영향에 대해 실험한 결과를 요약하면 다음과 같다. 1) 혈당과 뇨당의 변화 혈당은 부신절제로 인하여 감소하였다. 부신절제된 백서는 고탄수화물식이를 했을 경우 혈당이 가장 높았고, corticosterone을 투여하면 고지방, 고단백 식이를 했을 경우 혈당이 유의적으로 증가하였다 (p<0.05). aldosterone을 투여하면 고지방식이를 했을 경우 유의적으로 감소하였으나 다른 식이의 경우는 큰 변화가 없었다. 2) 인슐린과 c-peptide의 변화 인슐린 농도는 corticosterone을 투여하면 고탄수 화물식이와 고단백식이를 했을 경우 대조군보다 낮았으나 유의성은 보이지 않았다. c-peptide농도 역시 corticosterone을 투여하면 감소하였고, aldosterone 투여는 영향을 미치지 않았다. 3) 총콜레스테롤과 중성지발 함량 총콜레스테롤 함량은 부신절제로 인하여 감소하였다. 중성지방 함량은 corticosterone을 투여하면, 고지방, 고단백식이를 했을 경우 대조군에 비하여 유의적으로 증가하였다(p<0.05). 4) 체무게와 식이섭취량 체무게와 식이섭취량은 부신절제로 인하여 감소 하였으나, 고지방식이를 했을 경우는 부신젤제효과가 작았다. corticoxterone을 투여하면 체무게와 식이섭취량이 증가하며, aldosterone은 영향을 미치지 못했다. 식이효율은 aldosterone과 corticoxterone의 투여가 큰 영향을 미치지 않았다. 5) 장기무게 Corticoxterone의 투여로 간의 무게가 식이에 상관없이 증가하였다. 그외의 콩팥, 비장, 고환의 무게는 큰 변화가 없었다.

• 한국식품영양과학회지
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• 제42권10호
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• pp.1585-1591
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• 2013

본 연구의 목적은 고지방식으로 유도된 비만이 에너지 항상성 조절인자인 신경영양인자와 섭식조절 호르몬인 렙틴 mRNA 발현에 어떠한 관련성이 있는지를 규명하는 것과 비만 쥐의 시상하부 BDNF 발현과 렙틴 리셉터 mRNA 발현, 혈청 렙틴과 항비만과의 관계에 대한 운동트레이닝과 정상식이로의 전환에 대한 효과를 확인하고자 하였다. 그 결과 8주 후 비만 대조군(HF)에 비해 운동군(HFT), 정상식이군(HFND), 운동 및 정상식이 트레이닝군(HFNDT)에서 각각 유의한 체중 및 혈중 TC 감소를 보였다(P<0.05). 혈중 TG에서는 HF군에 비해 HFNDT군에서 유의한 감소를 보였다(P<0.05). 혈중 렙틴은 HF군에 비해 HFT군에서 유의한 감소를(P<0.05), HFT군에 비해 HFND군에서 유의한 감소를 보였으며(P<0.05), HFND군에 비해 HFNDT군에서 유의한 감소를 나타냈다(P<0.05). 시상하부 BDNF 단백질 발현은 HF군에 비해 모든 군에서 유의한 증가를 보였다(P<0.05). 시상하부 렙틴 리셉터 mRNA는 HF군에 비해 HFT와 HFND군에서 약간의 감소를, HFNDT군에서는 증가를 나타내었다. 이상의 결과는 고지방식이로 유도된 비만쥐에 대한 운동트레이닝은 항비만 효과가 있는 것으로 나타났으며, 운동 또는 정상식이의 병행은 식욕과 체중조절에 최적의 비만예방 및 개선효과가 있을 것으로 사료된다.

• Korean Journal of Acupuncture
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• 제26권1호
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• pp.85-109
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• 2009

Objective & Methods : The present study was to investigate the effect of Moutan Cortex Radicis Herbal-acupuncture (MCR-HA) at GB34 (Yangleungchean) on hyperlipidemia and arteriosclerosis induced by high fat diet in rats. Results : 1. Moutan Cortex Radicis herbal-acupuncture infusion solution (MCR-HAS) increased DPPH radical scavenging activity and HMG-CoA reductase inhibition rate in rat liver cells. 2. MCR-HA at GB34 decreased serum total cholesterol level, and increased serum phospholipid/total cholesterol in rats with high fat diet. 3. MCR-HA at GB34 decreased atherogenic index in rats with high fat diet. 4. MCR-HA at GB34 increased hepatic catalase activity in rats with high fat diet. Conclusion : These results suggested that Moutan Cortex Radicis herbal-acupuncture at GB34 has therapeutic effects on hyperlipidemia and related complications in rats with high fat diet.

• 대한의생명과학회지
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• 제12권4호
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• pp.319-327
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• 2006

This study aimed to determine whether troglitazone stimulates genes related to fatty acid ${\beta}$-oxidation, leading to modulation of white adipose tissue (WAT) metabolism in high fat diet-fed mice. Female C57BL/6J mice were randomly divided into two groups (n=10/group). After they received either a high fat diet or the same high fat diet supplemented with troglitazone for 4 weeks, the effects of troglitazone on gene expression and physiology of WAT were measured using Northern, histological and serological analyses. Administration of troglitazone induced the expression of genes involved in mitochondrial and peroxisomal fatty acid ${\beta}$-oxidation in mesenteric WAT. Troglitazone also significantly increased uncoupling protein 2 mRNA levels. The changes in WAT gene expression were accompanied by reductions in circulating levels of free fatty acids and triglycerides as well as glucose and insulin. Histological studies showed that troglitazone treatment decreased the average size of adipocytes in mesenteric WAT. These results suggest that troglitazone-stimulated WAT expression of genes associated with fatty acid ${\beta}$-oxidation regulates WAT metabolism of high fat diet-fed mice, contributing to improvement of insulin sensitivity.

• 한국식품영양학회지
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• 제29권3호
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• pp.411-419
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• 2016

In this study, we determined the effect of the mulberry leaf powder on blood lipid metabolism and liver function improvement of mice fed a high fat diet. The mulberry leaf showed a moisture content of $10.74{\pm}0.56%$, ash $9.67{\pm}0.56%$, crude protein $25.24{\pm}1.02%$, and crude fat $2.66{\pm}0.08%$. In the group with high fat diet supplemented with mulberry leaf powder, serum low-density lipoprotein (LDL-cholesterol) and total cholesterol levels were significantly lower (p<0.05); also, serum phospholipid and aspartate amino transferase (AST) levels were significantly lower (p<0.05). Serum insulin and leptin levels were high in mice fed a high fat diet; however, addition of mulberry leaf powder 10% in the diet had no significant effect on blood insulin and leptin control. The results of the study suggested that various plant sterols in mulberry leaf improve lipid metabolism and liver function in ICR-mice.

• 한방비만학회지
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• 제18권1호
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• pp.27-35
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• 2018

Objectives: The aim of this study is to investigate the effects of Daecheongryoung-tang (DCR) therapy on body weight, serum total cholesterol, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, triglyceride, free fatty acid, total lipid, phospholipid level and complete blood cell count of obese rats. Methods: 34 rats are divided into 4 groups, the rats in the normal group are 7 and the rats in the other group are 9 per group; Normal group (general fat diet and no medication), Control group (high-fat diet and no medication), DCR_L group (high-fat diet and DCR 250 mg medication) and DCR_H group (high-fat diet and DCR 500 mg medication). DCR is administrated for 6 weeks. Results: There is significant statistical difference between Control group and DCR-H group for the body weight, the total cholesterol, LDL cholesterol, triglyceride, free fatty acid level. Also, there is significant statistical difference among Control group, DCR_L group and DCR_H group for body weight, triglyceride, free fatty acid and phospholipid level. Conclusions: These results suggest that medication of DCR_L and DCR_H is effective for the treatment of obesity.