Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
권호 표지

Effects of Hyolbuchukeo-tang Extracts on Blood and liver of Hyperlipidemia Rats Induced by High Fat Diet

혈부축어탕(血府逐瘀湯) 추출물(抽出物)이 고지방식이(高脂肪食餌)로 유발(誘潑)시킨 고지혈증(高脂血症) 흰쥐의 혈액(血液) 및 간(肝) 기능(機能)에 미치는 영향(影響)

  • Pi, Chien-Mei (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University) ;
  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 필감매 (원광대학교 한의학전문대학원) ;
  • 정명수 (원광대학교 한의과대학) ;
  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 이기남 (원광대학교 한의학전문대학원)
  • Published : 2009.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The purpose of this study was to investigate the effects of Hyolbuchukeo-tang extracts on the hyperlipidemia rats induced by high fat diet. Methods and Materials: In vitro; The extracts prepared for Hyolbuchukeo-tang by hot water extraction (HH), fermentation(HF) and UMPM extraction(HU) method. The extracts were examined for levels of polyphenol compounds, antioxidant activities, and inhibitory potencies for HMG-CoA reductase. In vivo; Sprague-Dawley male rats of weighing $150{\pm}5g$ were randomly divided into six groups ; normal control diet(NC), and high fat diet(HC), high fat diet with treated lovastatin of 10mg/kg(PC), high fat diet with Hyolbuchukeo-tang extracts; HH, HF and HU treated extracts of 300mg/kg, respectively. Also, we compared the effects of the extracts of HH, HF and HU on rats fed high fat diet for four weeks. Results: 1. The content of polyphenol compounds and electron donating abilities(EDAs) was the HF higher than HH and HU. The superoxide dismutase(SOD)-like activities were proportionate in consistency and they appeared highly from all extracts. The HMG-CoA reductase inhibition activities was highest activities in the HU. 2. The activities of serum GOT and GPT were significantly lower in the HH and HF groups. The level of serum triglyceride was significantly decreased in the HF group. HH and HU groups were significantly decreased in the atherogenic index(AI). The total cholesterol concentration in liver was significantly decreased in the HF group, and HU showed more significantly decreased in the triglyceride than of the lovastatin. Also, photomicrographs of liver tissue showed higher fat accumulation in the HC group than in the HH, HF and HU groups. Conclusions: These result suggest that the hyper-lipidemia caused by a high fat diet was effectively inhibited the administration of HF and HU.

Keywords

References

  1. Kim JS : The current status of Korean coronary heart disease. In Diagnosis and treatment in hyperlipidemia. Association of hyperlipidemic therapy, Seoul, Haneuyhak. 2000, pp.64-74.
  2. Grundy SM, Denke MA: Dietary influence on serum lipids and lipoprotein. J Lipid Res. 1990 ; 31 : 1149-1172.
  3. 餘冬嚴, 魏康伯, 沃興德: 血府逐瘀湯對氣滯 血瘀型高脂血症降脂作用的臨床觀察與實驗 硏究, 中西醫結合雜誌. 1988 ; 8(10) : 601- 603.
  4. 鄭君莉: 水蛭粉治療高脂血症25例, 新中醫. 1985 ; 2 : 36-37.
  5. 丘萬嵩外: 消補減肥片對高脂血症影響的臨 床硏究, 中西醫結合雜誌. 1990 ; 10(9) : 532- 534.
  6. 胡曉晨外: 降脂膠囊治療高脂血症和對血液 流變學抗血小板聚集率臨床療效觀察, 中醫雜誌. 1988 ; 12 : 34-40.
  7. 寧選外: 山査降脂藥對中老年人高脂血症的 臨床觀察, 河南中醫. 1990 ; 10(4) : 23-24.
  8. 王勳臣編著: 醫林改錯, 서울, 醫聖堂. 1994 ; pp.61-65.
  9. 康舜洙: 韓醫學에서의 瘀血에 대한 槪念, 대한한의학회지. 1984 ; 5(1) : 138-140.
  10. 정우열, 안규석 : 韓方臨床病理學. 서울, 永林社. 1988 ; p.353.
  11. 尹吉榮: 東醫臨床方劑學. 서울, 明寶出版社. 1992 ; pp.265-266.
  12. 원광대학교 한의과대학 제18기 졸업준비위원회: 譯釋中醫方劑問答, 익산, 원광대학교 출판국. 1995 ; pp.539-540, 602-603.
  13. 강혜영, 강순수: 活血祛瘀劑에 관한 文獻的 考察, 대한한의학방제학회. 1991 ; 2(1) : 47-58.
  14. 최승훈, 안규석: Endotoxin으로 유발된 白鼠의 血栓症에 身痛逐瘀湯이 미치는 영향. 동의병리학회지. 1989 ; 4 : 47-56.
  15. 정찬길, 안규석, 문준전: 血栓症과 高粘度 血症에 미치는 黃芪, 桂枝및 紅花의 效能에 관한 實驗的硏究, 동의병리학회지. 1989 ; 4(1) : 74-92.
  16. American institute of nutrition : Report of the American institute of nutrition, Ad Hoc Committee on standards for nutritional studies., J Nutr. 1977 ; 107 : 1340-1343.
  17. AOAC : Official Methods of Analysis, 8th ed., Association of Official Analytical Chemists, Washington, D.C., U.S.A., 1955 ; p.144.
  18. Blois MS : Antioxidant determinations by the use of a stable free radical, J Agric Food Chem. 1977 ; 25 : 103-107. https://doi.org/10.1021/jf60209a051
  19. Kleinsek DA, Ranganathan S, Porter JW: Purification of 3-hydroxy-3- methylglutarylcoenzyme A reductase from rat liver, Methods in enzymeology. 1981 ; 71 : 462-479. https://doi.org/10.1016/0076-6879(81)71057-7
  20. Haglund O, Loustarinen F, Wallin F, Wicell I, Saldeen T: The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in mans supplemented with vitamin, Eur J Nutr. 1991 ; 121 : 165-172.
  21. Folch J, Lees M, Stanley GSH: A simple method for the isolation and purification of total lipid from animal tissues, J Bio Chem. 1957 ; 226 : 497-509.
  22. Fauci AS, Braunwald E, Isselbacher KJ, Longo DL: Harrison's principles of intermal medicine 14th ed. McGraw-Hill Hesgpth Professions Division, USA. 1994 ; p.2139.
  23. Robbins SL, Cotran R, Kumar V: Robbins pathologic basis of disease. 4th edition. W. B. Saunders. 1989 ; pp.556-561.
  24. Dalin R, Hiroyuki N, Hideomi A, Takahiro N: Study on Antihypertensive and Antihyperlipidemic effects of marine algae, Fisheries Science. 1994 ; 60(1) : 83-8.
  25. Hermier D, Hales P, Brindley DN: Effect of lipase inhibitor, Triton WR-1339 and tetrahydrolipstatin, on the synthesis and secretion of lipids by rat hepatocytes, FEBS Lett. 1991 ; 286(1,2) : 186-8. https://doi.org/10.1016/0014-5793(91)80970-E
  26. Kozarsky KF, McKinley DR, Austin LL, Raper SE, Stratford-Perricaudet LD, Wilson JM: In vivo correction of low density lipoprotein receptor deficiency in the Watanabe heritable hyperlipidemic rabbit with recombinant adenoviruses, J Bio Chem. 1994 ; 269(18) : 695-702.
  27. Noriaki K, Toru K, Atsushi M, Masayuki Y, Kenji I, Yutaka N, Chuichi K: Induction of mRNA for LDL receptors in heterozygous Watanabe Heritable Hyperlipidemic Rabbit treated with CS-514(Pravastatin) and Cholestyramine, Circulation. 1989 ; 79(5) : 1084-1090. https://doi.org/10.1161/01.CIR.79.5.1084
  28. Vushkin ML, Dolgov AV: Activity of cholesterol metabolism enzymes and lipid levels in the rat liver, aorta, adrenals and serum after exposure to Triton WR-1339, Vopr Med Khim. 1986 ; 32(3) : 98-101.
  29. 방혜정, 강윤호: 고지혈증의 처방에 관한 문헌적 고찰, 동서의학, 1996 ; 21(2) : 70-81.
  30. Kim HJ, Jun BS, Kim SY, Cha JY, Cho YS : Polyphenolic compound content and antioxidative acticities by extracts from seed, sprout and flower of Safflower(Carthamus tinctorius L.), J Korean Soc Food Sci Nutr. 2000 ; 29(6) : 1127-1132.
  31. Nice DJ, Robinson DS, Holden MA : Characterization of a heat stable antioxidant co purified with the superoxide dismutase activity from dried peas, Food Chem. 1995 ; 52 : 393-397. https://doi.org/10.1016/0308-8146(95)93288-3
  32. Kim SJ, Han D, Moon KD, Rhee JS : Measurement of superoxide dismutaselike activity of natural antioxidant, Biosci Biochem. 1995 ; 59(5) : 822-826. https://doi.org/10.1271/bbb.59.822
  33. Endo A: The discovery and development of HMG-CoA reductase inhibitors, J Lipid Res. 1992; 33 : 1569-1582.
  34. Siperstein MD, Fagna VM: Feedback control of mevalonate synthesis by dietary cholesterol, J Biol Chem. 1966 ; 241 : 602- 609.
  35. Brown MS, Dana SE, Dietschy JM, Siperstein MD: 3-Hydroxy-3-metylglutaryl coenzyme A reductase solubilization and purification of a cold-sensitive microsomal enzyme, J Biol Chem. 1973 ; 248 : 4731-4738.
  36. Hue FS, Krook L, Pomd WG, Duncan JR: Interactions of dietary calcium with toxic levels of lead and zinc in pigs, J Nutr. 1975 ; 105 : 112.
  37. Lim YK: Clinical application of biochemical test, Komoonsa, Seoul, 1980.
  38. Anber V, Griffin BA, McConnel M, Packard CJ, Shepherd J : Influence of plasma lipid and LDL-subfraction profile on the interaction between low density lipoprotein with human arterial wall proteoglycan, Atherosclerosis. 1996 ; 124 : 261-271. https://doi.org/10.1016/0021-9150(96)05842-X
  39. Austin MA, Rodriguez BL, McKnight B, McNeely MJ, Edwards KL, Curb JD, Sharp DS: Low-density lipoprotein particle size, triglycerides, and high-density lipoprotein cholesterol as risk factors of coronary heart disease in older Japanese- American men, Am J Cardiol. 2000 ; 86 : 412-416. https://doi.org/10.1016/S0002-9149(00)00956-5
  40. Guerin M, Bruckert E, Dolphin PJ, Turpin G, Chapman MJ : Feno-fibrate reduces plasma cholesterol ester transfer from HDL to VLDL and normalizes the atherogenic, dense LDL profile in combined hyperlipidemia, Arterioscler Thromb Vasc Biol. 1996 ; 16 : 763-772. https://doi.org/10.1161/01.ATV.16.6.763
  41. Packard CJ, Demant T, Stewart JP, Bedford D, Caslake MJ et al : Apolipoprotein- $\beta$ metabolism and the distribution of VLDL and LDL subfractions, J lipid Res. 2002 ; 41 : 305-318.
  42. 박정의: 관상동맥경화증 위험 인자의 역학 연구, 한국지질학회지 제 14차 추계학술대회, 1997 ; 7 : ss.101-108.
  43. SJ Rhee, HK Park : Changes of lipid content and histochemical observation in liver of rats fed high fat diet, Kor J Nutr. 1984 ; 17(2) : 113-125.
  44. Kang KJ, Lim SJ, Jeong JG, Han HK, Choi SS, kim MH, Kwon SY: Effects of was guard on weight, triglyceride, leptin and fat cell size in rats fed on a high fat diet, J Kor Nutr Soc. 2003 ; 36(5) : 446-451.