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Antioxidative and biological activity of extracts from Orostachys japonicus (와송(Orostachys japonicus) 추출물의 항산화 및 생리 활성)

  • Lim, Sun-Mi;Park, Hye-Jin;Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • v.60 no.4
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    • pp.293-300
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    • 2017
  • This study was designed to extracts from Orostachys japonicas were investigated to assess anti-oxidation and biological activity. Phenolic content was maximum of $10.56{\pm}0.32mg/g$ when extracted with 50% ethanol. In anti-oxidative activity, Orostachys japonicus electric donating activity was higher than 80% in both water and ethanol extract at $200{\mu}g/mL$. 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization of both water and ethanol extract was higher than 95.0% but antioxidant protection factor of water extract was higher than ethanol extract. Thiobarbituric acid reactive substance of ethanol extract was higher than water extract. For antihypertensive effect determination, angiotesin converting enzyme of water and ethanol extract showed 6.67 and 7.98% each at $200{\mu}g/mL$. Ethanol extract of $200{\mu}g/mL$ showed xanthin oxidase inhibitory effect of 60.85% but was not shown with water extract. Orostachys japonicus ethanol extract showed higher tyrosinase inhibitory activity of 64.59% which was higher than kojic acid of control indicating higher whitening effect. In anti-wrinkle effect, ethanol extract at $50-200{\mu}g/mL$ showed collagenase inhibitory effect of 75.95-85.02% which was higher than 68.91-76.64% of epigallocatechin-gallate of control group. 50% ethanol extract showed higher elastase inhibitory activity than water extract. Therefore, Orostachys japonicus extracts were identified to have high anti-wrinkle effect. These results identify anti-oxidative activity, gout prevention, whitening effect, and anti-wrinkle effect which indicate the possibility as a source for functional material.

A Survey on the Holding Amount of Cooking Tools of the Farm and Fishing Villages in Chungnam Area (충남지역 농어촌의 조리기구 보유실태에 관한 조사)

  • 김영인;김기숙
    • Korean journal of food and cookery science
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    • v.9 no.1
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    • pp.7-13
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    • 1993
  • This survey was performed to investigate the holding amount of the various kinds of cooking tools of the farm and fishing villages in Chungnam area. The results were as follows: 1. The holding ratio of refrigerators was the highest (97.9%), and that of toasters was the lowest (1.9%) among the electric tools. 2. The holding ratio of gas-ranges was the highest (95.7%) and that of gas oven-ranges was the lowest (1.2%) among 4 kinds of gas tools under investigation. 3. The holding amount of pans was the highest among the cooking tools and that of wicker baskets was very high among the small cooking tools. The holding ratios of egg-cutters and lemon-squeezers were very low (4.1% and 2.9%, respectively). 4. The holding ratios of various kinds of cooking tools were related to the housewife's educational background and family income.

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Estimations on the Water Purification of Forest by Analyzing Water Quality Variations in Forest Hydrological Processes (산지(山地) 물순환(循環) 소과정(素過程)에 있어서 수질변화(水質變化)의 추적분석(追跡分析)에 의한 산림(山林)의 환경적(環境的) 정화기능(淨化機能)의 계량화(計量化) 연구(硏究))

  • Lee, Heon-Ho
    • Journal of Korean Society of Forest Science
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    • v.86 no.1
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    • pp.56-68
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    • 1997
  • This study was carried out to evaluate the capacity of environment purification of forest and to reveal formation processes of stream water quality by evaluating water quality variations in forest hydrological processes. Water quantity, pH, electric conductivity(EC), dissolved oxygen(DO), and dissolved matter concentrations were monitored in open rainfall, throughfall, stemflow, litter flow and short-term stream flow for one unit storm, and also for those were monitored in long-term stream flow in Palgong, Yongsung, and Daedong catchments. The results were summarized as follows; 1. pH and DO values of stream flow were increased as the flux increased but EC values were decreased. 2. pH values of stemflow and throughfall were decreased with the lapse of rainfall time with lower values than open rainfall. Arid EC values were higher in initial rainfall period but lower gradually with the lapse of time than open rainfall. In litter flow, pH values were lower than open rainfall but EC values were higher. In stream flow, pH values of stream flow showed a high level in initial rainfall period and decreased remarkably with the lapse of time, but it recovered after the rainfall stopped. And however, the values of EC showed almost reverse tendency. DO values of litter flow and stream flow were decreased gradually with the lapse of time in litter flow and stream flow but there were no any tendency in open rainfall, stemflow and throughfall. 3. pH values of stemflow and throughfall in Quercus acutissima were higher than in Pinus densiflora, but EC values were lower. Total amount of canons in stemflow was higher in Pinus densiflora than in Quercus acutissima. 4. pH, DO, EC and total amount of cation values in hydrological processes were in the order of; litter flow

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Numerical Test for the 2D Q Tomography Inversion Based on the Stochastic Ground-motion Model (추계학적 지진동모델에 기반한 2D Q 토모그래피 수치모델 역산)

  • Yun, Kwan-Hee;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.10 no.3
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    • pp.191-202
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    • 2007
  • To identify the detailed attenuation structure in the southern Korean Peninsula, a numerical test was conducted for the Q tomography inversion to be applied to the accumulated dataset until 2005. In particular, the stochastic pointsource ground-motion model (STGM model; Boore, 2003) was adopted for the 2D Q tomography inversion for direct application to simulating the strong ground-motion. Simultaneous inversion of the STGM model parameters with a regional single Q model was performed to evaluate the source and site effects which were necessary to generate an artificial dataset for the numerical test. The artificial dataset consists of simulated Fourier spectra that resemble the real data in the magnitude-distance-frequency-error distribution except replacement of the regional single Q model with a checkerboard type of high and low values of laterally varying Q models. The total number of Q blocks used for the checkerboard test was 75 (grid size of $35{\times}44km^2$ for Q blocks); Q functional form of $Q_0f^{\eta}$ ($Q_0$=100 or 500, 0.0 < ${\eta}$ < 1.0) was assigned to each Q block for the checkerboard test. The checkerboard test has been implemented in three steps. At the first step, the initial values of Q-values for 75 blocks were estimated. At the second step, the site amplification function was estimated by using the initial guess of A(f) which is the mean site amplification functions (Yun and Suh, 2007) for the site class. The last step is to invert the tomographic Q-values of 75 blocks based on the results of the first and second steps. As a result of the checkerboard test, it was demonstrated that Q-values could be robustly estimated by using the 2D Q tomography inversion method even in the presence of perturbed source and site effects from the true input model.

Synthesis and Electrochemical Properties of (La0.6Sr0.4)(Co0.2Fe0.8)O3 cathode for SOFC on pH Control Using Modified Oxalate Method (Modified Oxalate Method 의해 합성한 SOFC용(La0.6Sr0.4)(Co0.2Fe0.8)O3 Cathode의 pH 변화에 따른 특성)

  • Lee, Mi-Jai;Choi, Byung-Hyun;Kim, Sei-Ki;Park, Sang-Sun;Lee, Kyung-Hee
    • Journal of the Korean Electrochemical Society
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    • v.10 no.4
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    • pp.288-294
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    • 2007
  • The LSCF cathode far Solid Oxide Fuel Cell was investigated to develop high performance unit cell at intermediate temperature by modified oxalate method with different electrolytes and different pH. The LSCF powders employed La, Sr, Co and Fe oxides, oxalic acid, ethanol and $NH_4OH$ solution were synthesized with pH controlled as 2, 6, 7, 8, 9 and 10 at $80^{\circ}C$ Single crystalline phase was obtained from pH $2{\sim}9$. on the other hand, $La_2O_3$ appeared from pH 10. Very fine powder with particle size of 50 nm was obtained at calcination temperature of $800^{\circ}C$ for 4 hours. LSCF cathode synthesized at pH 7 showed the highest electric conductivity in the temperature range of $600^{\circ}C$ to $900^{\circ}C$ its value was 950 S/cm at $900^{\circ}C$ Under same synthesis conditions, polarization resistance of each LSCF cathode was changed with different calcination temperatures. As-prepared powder presented 2.52, 1.54 and $2.58\;{\Omega}$ at $600^{\circ}C$ with ScSZ, 8Y-YSZ and GDC as its electrolyte respectively after calcination at $800^{\circ}C$ for 4 hours.

Recent Progress in Air-Conditioning and Refrigeration Research: A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2014 (설비공학 분야의 최근 연구 동향: 2014년 학회지 논문에 대한 종합적 고찰)

  • Lee, Dae-Young;Kim, Sa Ryang;Kim, Hyun-Jung;Kim, Dong-Seon;Park, Jun-Seok;Ihm, Pyeong Chan
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.27 no.7
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    • pp.380-394
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    • 2015
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2014. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) The research works on the thermal and fluid engineering have been reviewed as groups of heat and mass transfer, cooling and heating, and air-conditioning, the flow inside building rooms, and smoke control on fire. Research issues dealing with duct and pipe were reduced, but flows inside building rooms, and smoke controls were newly added in thermal and fluid engineering research area. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer and industrial heat exchangers. Researches on heat transfer characteristics included the results for thermal contact resistance measurement of metal interface, a fan coil with an oval-type heat exchanger, fouling characteristics of plate heat exchangers, effect of rib pitch in a two wall divergent channel, semi-empirical analysis in vertical mesoscale tubes, an integrated drying machine, microscale surface wrinkles, brazed plate heat exchangers, numerical analysis in printed circuit heat exchanger. In the area of pool boiling and condensing, non-uniform air flow, PCM applied thermal storage wall system, a new wavy cylindrical shape capsule, and HFC32/HFC152a mixtures on enhanced tubes, were actively studied. In the area of industrial heat exchangers, researches on solar water storage tank, effective design on the inserting part of refrigerator door gasket, impact of different boundary conditions in generating g-function, various construction of SCW type ground heat exchanger and a heat pump for closed cooling water heat recovery were performed. (3) In the field of refrigeration, various studies were carried out in the categories of refrigeration cycle, alternative refrigeration and modelling and controls including energy recoveries from industrial boilers and vehicles, improvement of dehumidification systems, novel defrost systems, fault diagnosis and optimum controls for heat pump systems. It is particularly notable that a substantial number of studies were dedicated for the development of air-conditioning and power recovery systems for electric vehicles in this year. (4) In building mechanical system research fields, seventeen studies were reported for achieving effective design of the mechanical systems, and also for maximizing the energy efficiency of buildings. The topics of the studies included energy performance, HVAC system, ventilation, and renewable energies, piping in the buildings. Proposed designs, performance performance tests using numerical methods and experiments provide useful information and key data which can improve the energy efficiency of the buildings. (5) The field of architectural environment was mostly focused on indoor environment and building energy. The main researches of indoor environment were related to the evaluation of work noise in tunnel construction and the simulation and development of a light-shelf system. The subjects of building energy were worked on the energy saving of office building applied with window blind and phase change material(PCM), a method of existing building energy simulation using energy audit data, the estimation of thermal consumption unit of apartment building and its case studies, dynamic window performance, a writing method of energy consumption report and energy estimation of apartment building using district heating system. The remained studies were related to the improvement of architectural engineering education system for plant engineering industry, estimating cooling and heating degree days for variable base temperature, a prediction method of underground temperature, the comfort control algorithm of car air conditioner, the smoke control performance evaluation of high-rise building, evaluation of thermal energy systems of bio safety laboratory and a development of measuring device of solar heat gain coefficient of fenestration system.

Effects of Diffused Light Materials on Marketable Yield and Quality of Sunlight Dried Red Pepper (Capsicum annuum L.) in Plastic Film House (비닐하우스 이용 태양초 생산시 산광 재료가 상품 수량과 품질에 미치는 영향)

  • Lee, Guang-Jae;Song, Myung-Gyu;Kim, Si-Dong;Nam, Sang-Young;Lee, Ki-Yeol;Kim, Tae-Jung;Kim, Dong-Eok;Yoon, Jung-Beom;Choi, Kyu-Hong
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.102-109
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    • 2014
  • This study was carried out to investigate the effects of diffused light materials on marketable yield and quality of sunlight dried red pepper in plastic film house. The after ripening methods were composed of 3 treatments; using electric bulk dryer (Bulk), sealing house (House), and PE sealing. The diffused light materials were control, tent, shading 35% (Shading net), white polypropylene (PP (white)), and black polypropylene (PP (Black)). The marketable yield was high in order of Bulk as 300 g, House as 275 g, and PE sealing as 112 g. The redness of PE sealing was the highest as 17.85 and that of Bulk was the lowest as 10.65. There was significant difference in redness among the treatments. The moisture content of red pepper was the most rapidly reduced in control, and was the most slowly reduced in Shading net treatment. The marketable yield index was the higher in PP (white) as 114% than control. The redness had a significant difference in PP (Black) and tent compared to the other treatments. The capsaicin content showed the highest as $160mg{\cdot}100g^{-1}$ in PP (white) treatment. Conclusively, the most effective method for sunlight drying red pepper was House with PP (White) treatment. It delayed drying for 2 days but increased marketable yield at 114% than control when sunlight dried red pepper in plastic house. Our results also provided an optimized method for improvement of drying red pepper with sunlight, and will be useful for further drying red pepper research.

Reaction Gas Composition Dependence on the Properties of SnO2 Films on PET Substrate by ECR-MOCVD (반응가스조성이 PET기판위에 ECR 화학증착법에 의해 제조된 SnO2 박막특성에 미치는 영향)

  • Kim, Yun-Seok;Lee, Joong-Kee
    • Journal of the Korean Electrochemical Society
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    • v.8 no.3
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    • pp.139-145
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    • 2005
  • [ $SnO_x$ ] films on the flexible substrate of PET film were prepared at ambient temperature under a $(CH_3)_4Sn(TMT: tetra-methyl tin)-H_2-O_2$ atmosphere in order to obtain transparent conductive polymer by using ECR-MOCVD(Electro Cyclotron Resonance Metal Organic Chemical Yfpor Deposition) system. The prepared $SnO_x$ thin films show generally over $90\%$ of optical transmittance at wavelength range of 380-780nm and about $1\times10^{-2\~3}ohm{\cdot}cm$ of electrical resistivity. In the present study, effects of $O_2/TMT\;and\;H_2/TMT$ mole ratio on the properties of $SnO_x$ films are investigated and the other process parameters such as microwave power, magnetic current power, substrate distance and working pressure are fixed. Based on our experimental results, the $SnO_x$ film composition ratio of Sn and O directly influences on the electrical and optical properties of the films prepared. The $SnO_x$ film with low electric resistivity and high transmittance could be obtained by controlling the process parameters such as $O_2/TMT\;and\;H_2/TMT$ mole ratio, which play an important role to change the composition ratio between Sn and O. An increase of $O_2/TMT$ mole ratio brought on the increases 0 content in the $SnO_x$ film. On the other hand, an increase of $H_2/TMT$ mole ratio lead to decreases the oxygen content in the film. The optimized composition ratio of oxygen : tin Is determined as 2.4: 1 at $O_2/TMT$ of 80 and $H_2/TMT$ of 40 mole ratio, respectively.

Effect of Supplementary Radiation on Growth of Greenhouse-Grown Kales (온실재배 케일의 생장에 미치는 보광효과)

  • Heo, Jeong-Wook;Kim, Hyeon-Hwan;Lee, Kwang-Jae;Yoon, Jung-Boem;Lee, Joung-Kwan;Huh, Yoon-Sun;Lee, Ki-Yeol
    • Korean Journal of Environmental Agriculture
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    • v.34 no.1
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    • pp.38-45
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    • 2015
  • BACKGROUND: For commercial production of greenhouse crops under shorter day length condition, supplementary radiation has been usually achieved by the artificial light source with higher electric consumption such as high-pressure sodium, metal halide, or incandescent lamps. Light-Emitting Diodes (LEDs) with several characteristics, however, have been considered as a novel light source for plant production. Effects of supplementary lighting provided by the artificial light sources on growth of Kale seedlings during shorter day length were discussed in this experiment. METHODS AND RESULTS: Kale seedlings were grown under greenhouse under the three wave lamps (3 W), sodium lamps (Na), and red LEDs (peak at 630 nm) during six months, and leaf growth was observed at intervals of about 30 days after light exposure for 6 hours per day at sunrise and sunset. Photosynthetic photon flux (PPF) of supplementary red LEDs on the plant canopy was maintained at 0.1 (RL), 0.6 (RM), and $1.2(RH){\mu}mol/m^2/s$ PPF. PPF in 3 W and Na treatments was measured at $12{\mu}mol/m^2/s$. Natural light (NL) was considered as a control. Leaf fresh weight of the seedlings was more than 100% increased under the 3 W, Na and RH treatment compared to natural light considering as a conventional condition. Sugar synthesis in Kale leaves was significantly promoted by the RM or RH treatment. Leaf yield per $3.3m^2$ exposed by red LEDs of $1.2{\mu}mol/m^2/s$ PPF was 9% and 16% greater than in 3W or Na with a higher PPF, respectively. CONCLUSION: Growth of the leafy Kale seedlings were significantly affected by the supplementary radiation provided by three wave lamp, sodium lamp, and red LEDs with different light intensities during the shorter day length under greenhouse conditions. From this study, it was suggested that the leaf growth and secondary metabolism of Kale seedlings can be controlled by supplementary radiation using red LEDs of $1.2{\mu}mol/m^2/s$ PPF as well as three wave or sodium lamps in the experiment.

Electrofusion of Yeast Cells and Their Genetic Analysis Using RAPD-PCR (효모세포의 전기융합 및 융합세포의 RAPD-PCR을 이용한 유전적 분석)

  • Kim, Seung;Kim, Jae-Sung;Sapkota, Kumar;Park, In-Sung;Cho, Moon-Gu;Park, Yeal;Chun, Hong-Sung;Choi, Bong-Suk;Park, Se-Eun;Choi, Han-Suk;Kim, Myung-Kon;Kim, Sung-Jun
    • Applied Biological Chemistry
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    • v.49 no.3
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    • pp.186-191
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    • 2006
  • In the present study, an attempt has been made to produce hybrid yeast strains of different useful and dominant characteristics. The hybrid yeast strains were produced by electrofusion and their genetic analysis were performed by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction). The protoplast of Saccharomyces cerevisiae KCTC 7904 and Zygosaccharomyces rouxii KCTC 7966 were obtained above 92% when treated with lyticase at $30^{\circ}C$ for $60{\sim}90$ min after the pretreatment of $1{\sim}2%$ 2-mercaptoethanol at $30^{\circ}C$ for $15{\sim}20$ min. The fusant was produced from paired protoplast stage under the electric pulse at high frequency conditions (1.5 MHz/50 pV, 615 $V/256\;{\mu}sec$) within glass-platinum made electrofusion chamber. Changes in RAPD patterns in mother cells and hybrid cells proved that the fusant contains two types of yeast gene originated from its parent. Furthermore, fermentation characters exhibits by the fusant cell confirmed its genetic changes. These results suggest that genetically stable hybrid yeast strains of economic importance can be produced by electrofusion technique and these electrofused yeast cells have an enormous impact in biotechnology and biomedicine.