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Existence of Cholinergic and Purinergic Receptor on the Detrusor Muscle of Rat Urinary Bladder (흰쥐 적출 배뇨근에서 콜린성 및 퓨린성 수용체의 존재)

  • Choi, Tae-Su;Kwon, Oh-Cheol;Ha, Jeoung-Hee;Lee, Kwang-Youn;Kim, Won-Joon
    • Journal of Yeungnam Medical Science
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    • v.8 no.2
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    • pp.138-149
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    • 1991
  • This study was aimed at investigation of the stimulatory innervations on the rat urinary bladder. Detrusor muscle strips of 15 mm long were suspended in isolated muscle chambers containing 1 ml of PSS maintained at $37^{\circ}C$ and aerated with 95% $O_2/5%CO_2$. Isometric myography was perfomed, and the results were as followings : Muscle strips showed "on-contraction" by electric field stimulation (EFS) frequency-dependently. The EFS-induced contraction was not affected by hexamethonium, a ganglion blocker, but abolished, by tetrodotoxin, a nerve conduction blocker. Physostigmine, a cholinesterase inhibitor enhanced the EFS-induced contraction which was inhibited by hemicholinium, an inhibitor of choline uptake at the cholinergic nerve ending. Such an EFS-induced contraction was antagonized by atropine only partially, and the atropine-resistant portion was completely abolished by the desensitization of purinergic receptors by prolonged incubatin of the strips in the presence of high concentratin of ATP. Bethanechol, a cholinergic agonist, elicited concentration-dependent contraction. Adenosine triphosphate (ATP), a purinergic agonist, induced a weak but concentration-dependent contraction of short duration. Bethanechol-induced contraction was not affected by ATP-desensitization, and ATP-induced contraction was not affected by tetrodotoxin. These results suggest that there are at least two main stimulatory components of innervations in the detrusor muscle, cholinergic muscarinic and purinergic ; and those receptors are independent each other.

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An Analysis on the Educational Needs for the Smart Farm: Focusing on SMEs in Jeon-nam Area (중소·중견기업의 스마트팜 교육 수요 분석: 전남지역을 중심으로)

  • Hwang, Doo-hee;Park, Geum-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.649-655
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    • 2020
  • This study determined effective educational strategies by investigating and analyzing the related educational demands for SMEs (small and medium-sized enterprises) in the 4th Industrial Revolution based area of smart farms. In order to derive the approprate educational strategies, Importance-Performance Analysis (IPA) and Borich's Needs Assessment Model were conducted based on the smart farm technological field. As a result, the education demand survey showed high demand for production systems and intelligent farm machinery. In detail, Borich's analysis showed the need for pest prevention and diagnosis technology (8.03), network and analysis SW linkage technology (7.83), and intelligent farm worker-agricultural power system-electric energy hybrid technology (7.43). In contrast, smart plant factories (4.09), lighting technology for growth control (4.46) and structure construction technology (4.62) showed low demands. Based on this, the IPA portfolio shows that the network and analysis SW linkage technology and the CAN-based complex center are urgently needed. However, the technology that has already been developed, such as smart factory platform development, growth control lighting technology and structure construction technology, was oversized. Based on these results, it is possible to strategically suggest the customized training programs for industrial sectors of SMEs that reflect the needs for efficiently operating smart farms. This study also provides effective ways to operate the relevant training programs.

The Study about Improvement of Neuro Energy Decreased by Energy Saving (에너지절감에 의해 감소되는 뉴로에너지의 증강에 관한 연구)

  • Kim, Myung-Ho;Kang, Dong-Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.715-721
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    • 2018
  • This study examined energy saving and elevating the worker's neuro energy (comfort, concentration, physical, and psychological stability) by compensating for the unpleasant tactile sensation to stimulate auditory and olfactory senses and reduce energy consumption. The experiment was conducted in an environment test room under the test conditions of temperature $25[^{\circ}C]$, relative humidity 50[RH%], illumination 1,000[lux] and air current speed 0.02[m/sec] by stimulating the auditory senses with a 1/f change in rhythm and the olfactory senses with an aroma scent. The experiment utilized the method of EEG, which evaluates human body's psychological status via tactile means, and the method of the vibra image, which evaluates the learning abilities, HRV and human body's psychological status via non-tactile means. The subjects were selected as eight university students (four males and four females) in their 20s, the type that have high relative ${\alpha}$(8~13[Hz]) activation in occipital lobe, which brings the highest level of mind stability and concentration, who had no difficulty in physical activities. The subjects' posture and physical activity was fixed to 1met - when the subjects are seated and relaxing in a comfortable environment - and their clothes condition was standardized as 0.7clo. As a result, the sentimental and psychological stability and concentration were the highest in the multisensory stimulation of jasmine scent and change rhythm of an a=1.106 sound source. In addition, under this condition, the relative $M{\alpha}$ and relative $M{\beta}$ increased by 70.49[%] and 89.72[%], respectively; the HRT decreased by 39.09[%]; and the fatigue and tension/anxiety decreased by 36.85[%] and 15.54[%], respectively.

Influence of temperature gradient induced by concentrated solar thermal energy on the power generation performance of a thermoelectric module (집중 태양열에 의한 온도구배가 열전발전모듈의 출력 성능에 미치는 영향)

  • Choi, Kyungwho;Ahn, Dahoon;Boo, Joon Hong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.10
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    • pp.777-784
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    • 2017
  • Energy harvesting through a thermoelectric module normally makes use of the temperature gradient in the system's operational environment. Therefore, it is difficult to obtain the desired output power when the system is subjected to an environment in which a low temperature gradient is generated across the module, because the power generation efficiency of the thermoelectric device is not optimized. The utilization of solar energy, which is a form of renewable energy abundant in nature, has mostly been limited to photovoltaic solar cells and solar thermal energy generation. However, photovoltaic power generation is capable of utilizing only a narrow wavelength band from the sunlight and, thus, the power generation efficiency might be lowered by light scattering. In the case of solar thermal energy generation, the system usually requires large-scale facilities. In this study, a simple and small size thermoelectric power generation system with a solar concentrator was designed to create a large temperature gradient for enhanced performance. A solar tracking system was used to concentrate the solar thermal energy during the experiments and a liquid circulating chiller was installed to maintain a large temperature gradient in order to avoid heat transfer to the bottom of the thermoelectric module. Then, the setup was tested through a series of experiments and the performance of the system was analyzed for the purpose of evaluating its feasibility and validity.

커패시터에의 적용을 위해 PET 필름에 스퍼터 증착한 ZrO2 박막의 특성

  • Gwon, Neung;Fei, Chen;Ryu, Han;Park, Sang-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.389.1-389.1
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    • 2014
  • 최근의 환경 및 에너지에 대한 관심으로 수요가 증가하고 있는 하이브리드 및 전기 자동차나 태양광발전, 풍력발전용의 인버터기기에는 고에너지밀도 커패시터가 필수적이 되었다. 높은 에너지 밀도를 요구하는 전력전자, 펄스파워 등의 응용분야에 사용되는 고에너지밀도 커패시터는 PET (Polyethylene terephtalate)와 PP (Polypropylene)와 같은 폴리머 유전체를 사용하는 범용 필름 커패시터가 사용되었으나 사용 요구 조건의 한계에 도달하여, 새로운 유전체를 적용하는 커패시터가 절실히 필요한 상황이다. PET와 PP와 같은 유전체는 유전상수가 2~3의 낮은 값을 가지고 있어 고에너지밀도를 구현하기가 어렵다. 본 연구에서는 새롭게 요구되고 있는 고에너지 밀도 커패시터의의 성능을 만족시키기 위하여 $20{\sim}50{\mu}m$ 두께의 PET 필름상에 세라믹 유전체인 $ZrO_2$ 박막을 스퍼터(Sputter) 증착법에 의해 코팅하여 종래의 필름 커패시터와 세라믹 커패시터의 장점을 갖는 커패시터를 제조하기 위한 박막 유전재료의 개발을 목표로 하였다. 수백 nm~수 ${\mu}m$ 두께의 $ZrO_2$ 박막을 스퍼터링 공정조건에 따라 증착한 후 박막의 결정성, 기판과의 부착성, 증착속도, 유전상수, 절연파괴강도, 온도안정성 등을 XRD, SEM, AFM, EDS, XPS, Impedance analyzer 등에 의해 평가하였다. $ZrO_2$ 유전체막은 상온에서 증착하였음에도 정방정(tetragonal)구조의 결정질로 성장하였고 증착압력이 증가함에 따라 주피크의 세기가 감소하였다. 증착 중 산소가스를 주입하였을 경우에도 결정질막으로 성장하였다. 증착막들은 산소가스의 양이 증가함에 따라 짙은 흰색으로 변하였으며 PET 기판과의 접착력도 약해졌다. 또한 거칠기는 Ar가스만으로 증착한 경우보다 증가하였으며 24~66 nm의 평균 거칠기값을 보였다. PET위에 Ar가스만으로 증착한 $ZrO_2$의 비유전율은 1kHz에서 116~87의 비유전율을 보여 PET에 비해 매우 우수한 특성을 보였다. $ZrO_2$ 막들은 300kV/cm의 전계에서 대략 10-8A 이하의 누설전류를 보였다. 증착가스비를 달리하여 제조된 시편에서도 유사한 누설전류값을 나타내었다. 300 kV/cm 전후의 전계까지 측정한 $ZrO_2$ 막의 P-E (polarization-electric field) 특성을 확인하였는데, 5 mTorr의 압력에서 증착한 막은 253 kV/cm에서 $5.5{\mu}C/cm^2$의 분극값을 보였다. P-E커브의 기울기와 분극량에 따라 에너지밀도가 달라지므로 공정조건에 따라 에너지밀도가 변화됨을 예측할 수 있었다. PET위에 스퍼터 증착한 $ZrO_2$ 유전체막은 5mTorr의 Ar가스분위기에서 제조할 때 가장 안정적인 구조를 보였으며, 고에너지밀도 커패시터에의 적용가능성을 보였다.

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Effects of Argon-plasma Jet on the Cytoskeleton of Fibroblasts: Implications of a New Approach for Cancer Therapy (Fibroblasts 세포주의 세포골격에서 아르곤 플라즈마의 효과: Cancer Therapy의 새로운 접근방법)

  • Han, Ji-Hye;Nam, Min-Kyung;Kim, Yong-Hee;Park, Dae-Wook;Choi, Eun Ha;Rhim, Hyangshuk
    • KSBB Journal
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    • v.27 no.5
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    • pp.308-312
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    • 2012
  • Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.

A Study of High-Precision Time-Synchronization for TDoA-Based Location Estimation (TDoA 기반의 위치 추정을 위한 초정밀 시각동기에 관한 연구)

  • Kim, Jae Wan;Eom, Doo Seop
    • KIPS Transactions on Computer and Communication Systems
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    • v.2 no.1
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    • pp.7-14
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    • 2013
  • Presently, there are many different technologies used for position detection. However, as signal-receiving devices operating in different locations must detect the precise position of objects located at long distances, it is essential to know the precise time at which an object's or a user's terminal device sends a signal. For this purpose, the existing time of arrival (ToA) technology is not sufficiently reliable, and the existing time difference of arrival (TDoA) technology is more suitable. If a TDoA-based electric surveillance system and other tracking devices fail to achieve precise time-synchronization between devices with separation distance operation, it is impossible to obtain correct TDoA values from the signals sent by the signal-receiving devices; this failure to obtain the correct values directly affects the location estimation error. For this reason, the technology for achieving precise time synchronization between signal-receiving devices in separation distance operation, among the technologies previously mentioned, is a core technology for detecting TDoA-based locations. In this paper, the accuracy of the proposed time synchronization and the measurement error in the TDoA-based location detection technology is evaluated. The TDoA-based location measurement error is significantly improved when using the proposed method for time-synchronization error reduction.

A Study of Electromagnetic Actuator for Electro-pneumatic Driven Ventricular Assist Device

  • Jung Min Woo;Hwang Chang Mo;Jeong Gi Seok;Kang Jung Soo;Ahn Chi Bum;Kim Kyung Hyun;Lee Jung Joo;Park Yong Doo;Sun Kyung
    • Journal of Biomedical Engineering Research
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    • v.26 no.6
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    • pp.393-398
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    • 2005
  • An electromechanical type is the most useful mechanism in the various pumping mechanisms. It, however, requires a movement converting system including a ball screw, a helical cam, or a solenoid-beam spring, which makes the device complex and may lessen reliability. Thus, the authors have hypothesized that an electromagnetic actuator mechanism can eliminate the movement converting system and that thereby enhance the mechanical reliability and operative simplicity of an electro­pneumatic pump. The purpose of this study was to show a novel application of electromagnetic actuator mechanism in pulsatile pump and to provide preliminary data for further evaluations. The electromagnetic actuator consists of stators with a single winding excitation coil and movers with a high energy density neodymium-iron-boron permanent magnet. A 0.5mm diameter wire was used for the excitation coil, and 1000 turns were wound onto the stators core with parallel. A prototype of extracorporeal electro-pneumatic pump was constructed, and the pump performance tests were performed using a mock system to evaluate the efficiency of the electromagnetic actuator mechanism. When forward and backward electric currents were supplied to the excitation coil, the mover effectively moved back and forth. The nominal stroke length of the actuator was 10mm. The actuator dimension was 120mm in diameter and 65mm in height with a mass of 1.4kg. The prototype pump unit was 150mm in diameter, 150mm in thickness and 4.5kg in weight. The maximum force output was 70N at input current of 4.5A and the maximum pump rate was 150 beats per minute. The maximum output was 2.0 L/minute at a rate of 80bpm when the afterload was 100mmHg. The electromagnetic actuator mechanism was successfully applied to construct the prototype of extracorporeal electro­pneumatic pump. The authors provide the above results as a preliminary data for further studies.

Habitat Characteristics and Distribution of Cymbidium kanran Native to Jejudo, Korea (제주한란의 자생환경 특성 및 분포에 관한 연구)

  • Lee, Jong Suk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.1
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    • pp.40-49
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    • 2004
  • This work carried out to define the characteristic of Cymbidium kanran habitat at Mt. Halla in Jejudo, Korea from the ecological point of view including geological and topographical features, air and soil temperature, relative humidity, fluctuations of light intensity, habitat vegetation, distribution altitude, area limit, and etc. And another goal of this study consider the conservation counterplan of the cymbidium habitat. Natural distribution areas of the cymbidium were observed more abundantly on the well drained south and east-facing slopes. Soil acidity was ranged from pH 4.1 to 5.3, and electric conductivity was ranged from 176.4 to 299.9 us/cm (average 215.3 us/cm). Base-saturation percentage of the habitat soil was below 50%, bulk density 0.42g/$cm^2$, particle density 2.05g/$cm^2$, humus content 26%, total nitrogen 0.82%, available phosphate 4.2 mg/kg, exchangeable potassium 0.63 Cmol/kg, calcium 0.44 Cmol/kg and magnesium 0.67 Cmol/kg. Annual mean air temperature was $15.4^{\circ}C$, however, air temperature was ranged $11.7{\sim}18.2^{\circ}C$ in spring, $21.2{\sim}23.8^{\circ}C$ in summer, $12.8{\sim}22.0^{\circ}C$ in fall and $5.5{\sim}7.8^{\circ}C$ in winter season. Annual mean soil temperature at depth of 10cm was $13.2^{\circ}C$ And minimum value was recorded $4.7^{\circ}C$ on January, and maximum value $22.5^{\circ}C$ on August. Relative humidity was ranged 90.8~94.7% in summer, 80.8~91.5% in fall and 77.6~84.2% in winter season. Minimum value was 56.5% on December, and maximum value was 100% on July and August. Light intensities were ranged from 400 to 1,800 lux at the greater part of Cymbidium kanran sites in Jejudo. Summer regarded as an lower light intensities was recorded to be range of 500~600 lux; however, autumn and winter were shown higher light regimes ranged from 3,500 to 3,800 lux. Therefore, one must be suprised that the cymbidium grow at the light condition of 6 lux (minimum) or 10,000 lux (maximum). Tree species keeping higher frequency rate and density were Eurya japonica, Camellia japonica, Castanopsis cuspidata, Carpinus laxiflora and Pinus densiflora. Number of trees growing in a 5${\times}$5m quardrat was 35 as an average, and proportion of evergreen versus deciduous was 5:1. Distribution altitude of the orchid habitat was ranged from 120m (low) to 840m (high) from sea level on the south facing slope of Mt. Halla, and was ranged eastern borderline of Gujwaup, Bukjejugun to western boundaries of Jungmundong, Seogwipo city. For the stable conservation of Cymbidium kanran habitat, sunlight regimes must be increased more by means of cutting trees or twigs in the site.

A Study on Energy-saving Landscape Facilities through a Park User's Attitude Survey - Focused on Urban Parks in Gimpo - (공원이용자 의식조사를 통한 에너지 절약형 조경시설물 도입에 대한 연구 - 김포시 근린공원을 중심으로 -)

  • Doo, Chul-Eon;Lee, Jae-Keun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.3
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    • pp.17-28
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    • 2012
  • This study aims to present the ground for the introduction of energy-saving landscape facilities which can meet the requirements of the age and create a new space by introducing the facilities which saves energy and that users prefer at the space where urbanites enjoy leisure activities in the urban parks. Thus, in addition to a spatial analysis of the parks for 10 urban parks in Gimpo, through an attitude survey on the users' behaviors, the following conclusions were drawn out. First, as a result of putting all facilities in the urban parks in Gimpo, on average, 6.9 out of the standards of 9 (76.6%) have been installed, and as a result of an analysis on the total number of the installed facilities, on average, about 28 kinds of facilities have been installed, so only 22.4% of the total 125 facilities were installed. Second, many urbanites are using the parks for the purpose of taking a walk and exercise to improve their health; however, the legal standards regulate only 2.9 exercise facilities on average in the target area, which are somewhat insufficient. Third, regarding the citizens' intent to participate in responding to climate change, 76% of them showed their intention of participation. Thus, if the energy-saving facilities, the purpose of this study, are introduced, many users will use them, and it is judged that costs for existing street lights and electric power plants will decrease. Fourth, as a result of an analysis of their satisfaction with the facilities, the value of R-square meaning the explanatory power of the independent variables put in a linear model for dependent variables was 0.860, approximately 81% of the total satisfaction, which was a high explanatory power. This study conducted an attitude survey on the users of urban parks in Gimpo at the dimensions of the introduction of energy-saving facilities in the urban parks, the national policies and responses to climate change. It is significant that it identified the appropriateness of developing much more energy-saving landscape facilities needed for low-carbon green growth in various types, and as a subsequent project, a more precision study on this is necessary continuously.