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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Argon-plasma Jet on the Cytoskeleton of Fibroblasts: Implications of a New Approach for Cancer Therapy

Fibroblasts 세포주의 세포골격에서 아르곤 플라즈마의 효과: Cancer Therapy의 새로운 접근방법

  • Han, Ji-Hye (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea) ;
  • Nam, Min-Kyung (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea) ;
  • Kim, Yong-Hee (Plasma Bioscience Research Center, Kwangwoon University) ;
  • Park, Dae-Wook (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea) ;
  • Choi, Eun Ha (Plasma Bioscience Research Center, Kwangwoon University) ;
  • Rhim, Hyangshuk (Department of Medical Life Sciences, Department of Biomedical Sciences, College of Medicine, the Catholic University of Korea)
  • 한지혜 (가톨릭대학교 의생명과학교실) ;
  • 남민경 (가톨릭대학교 의생명과학교실) ;
  • 김용희 (광운대학교 플라즈마 바이오과학 연구센터) ;
  • 박대욱 (가톨릭대학교 의생명과학교실) ;
  • 최은하 (광운대학교 플라즈마 바이오과학 연구센터) ;
  • 임향숙 (가톨릭대학교 의생명과학교실)
  • Received : 2012.09.28
  • Accepted : 2012.10.11
  • Published : 2012.10.31
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Abstract

Argon-plasma jet (Ar-PJ) is generated by ionizing Ar gas, and the resulting Ar-PJ consists of a mixture of neutral particles, positive ions, negative electrons, and various reactive species. Although Ar-PJ has been used in various biomedical applications, little is known about the biological effects on cells located near the plasma-exposed region. Here, we investigated the effects of the Ar-PJ on actin cytoskeleton of mouse embryonic fibroblasts (MEFs) in response to indirect as well as direct exposure to Ar-PJ. This Ar-PJ was generated at 500 mL/min of flow rate and 100 V electric power by our device mainly consisting of electrodes, dielectrics, and a high-voltage power supply. Because actin cytoskeleton is the key cellular machinery involved in cellular movement and is implicated in regulation of cancer metastasis and thus resulting in a highly desirable cancer therapeutic target, we examined the actin filament architectures in Ar-PJ-treated MEFs by staining with an actin-specific phalloidin labeled with fluorescent dye. Interestingly, the Ar-PJ treatment causes destabilization of actin filament architectures in the regions indirectly exposed to Ar-PJ, but no differences in MEFs treated with Ar gas alone and in untreated cell control, indicating that this phenomenon is a specific cellular response against Ar-PJ in the live cells, which are indirectly exposed to Ar-PJ. Collectively, our study raises the possibility that Ar-PJ may have potential as anti-cancer drug effect through direct destabilization of the actin cytoskeleton.

Keywords

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