• 약학회지
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• 제54권1호
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• pp.55-61
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• 2010

Gardenia jasminoides is one of the most widely used herbal preparations for the treatment of liver disorders. This study evaluated the potential beneficial effect of G. jasminoides in a mouse model of carbon tetrachloride ($CCl_4$)-induced liver injury. The mice were treated intraperitoneally with $CCl_4$ (10 ${\mu}l$/kg). They received G. jasminoides (30, 100, 300 mg/kg) 48 h, 24 h and 2 h before and 6 h after administering $CCl_4$. The serum activities of aminotransferase and the hepatic level of malondialdehyde were significantly higher 24 h after the $CCl_4$ treatment, while the concentration of reduced glutathione was lower. These changes were attenuated by G. jasminoides. $CCl_4$ increased the level of circulating tumor necrosis factor-$\alpha$ (TNF-$\alpha$) markedly, which was reduced by G. jasminoides. The levels of hepatic inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression were markedly higher after the $CCl_4$ treatment. G. jasminoides diminished these alterations. $CCl_4$ increased the level of TNF-$\alpha$, iNOS and COX-2 mRNA expressions, and these increases were attenuated by G. jasminoides. These results suggest that G. jasminoides alleviates $CCl_4$-induced liver injury, and this protection is likely due to the reduced oxidative stress and the downregulation of proinflammatory mediators.

• Natural Product Sciences
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• 제14권2호
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• pp.95-99
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• 2008

In a project to study the hepatroprotective effect of some plant extracts four plants Ephedra foliate Boiss, Alhagi maurorum Medikus, Capsella bursa-pastoris (L.) Medik. and Hibiscus sabdariffa L. were studied. The ethanol extract of the aerial part of the first three plants and the flowers of H. sabdariffa were subjected to hepatoprotective assays using Wistar albino rats. Liver injury induced in rats using carbon tetrachloride. The biochemical parameters; serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin were estimated as reflection of the liver condition. Based on the good results of the biochemical parameters measurements, histopathological study was performed on the liver of rats treated with E. foliate. The normal appearance of hepatocytes indicated a good protection of the extract from carbon tetrachloride hepatotoxicity. All the results were compared with silymarin, the reference hepatoprotective drug.

• Environmental Analysis Health and Toxicology
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• 제14권1_2호
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• pp.13-19
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• 1999

Cisplatin is an inorganic complex formed by a central atom of platinum surrounded by chlorine and ammonia atoms in the cis position in the horizontal plane, Cisplatin is one of the most effective anticancer drug, widely used against various tumor such as testicular tumor, brain tumor, ovary tumor, bladder carcinoma, colon cancer etc. However its clinical use has been limited by nephrotoxicity, ototoxicity , gastrointestinal disturbances, myeloscrppression and allergic reactions. In these toxicities, dose related and cumulative nephrotoxicity is the major dose limit factor. So, to evaluate the protective effect of aspalactone on cisplatin nephrotoxicity in rats, both compounds were given intraperitoneally, Protective effects of aspalactone against nephrotoxicity of cisplatin were observed when aspalactone was administered to rats 1hr beforecisplatin injection. Hepatotoxicity induced by combination treatment of cisplatin and aspalactone was not observed. The present results indicate that aspalactone may provide protection against cisplatin nephrotoxicity, when it is given 1hr before cisplatin injection.

• Advances in Traditional Medicine
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• 제10권2호
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• pp.79-85
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• 2010

The methanolic extract of natural roots of Mucuna pruriens was screened for hepatoprotective and antioxidant effects in carbon tetrachloride ($CCl_4$) induced hepatotoxicity in Albino rats. The degree of protection was measured by estimating biochemical parameters like serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, total protein and level of serum bilirubin (both total and direct). The extract also reduced $CCl_4$ induced lipid peroxidation both in-vitro and in-vivo. Hepatoprotective activity of methanolic extract at dose 300 mg/kg body weight, i.p., was comparable with standard drug Silymarin (25 mg/kg, i.p.). Furthermore, histopathological experiments were also carried out to support the study.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.175.1-175.1
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• 2003

The protective effects of acteoside, a phenylethanoid glycoside, on carbon tetrachloride-induced hepatotoxicity and the possible mechanisms involved in this protection were investigated in mice. Pretreatment with acteoside prior to the administration of carbon tetrachloride significantly prevented the increased serum enzymatic activities of alanine and aspartate aminotransferase in a dose-dependent manner. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.290.2-290.2
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• 2002

The protective effects of a Platycodi Radix (Changkil: CK), the root of Platycodon grandiflorum A. DC (Campanulaceae). on carbon tetrachloride-induced hepatotoxicity and the possible mechanisms involved in this protection were investigated in mice. Pretreatment with CK prior to the administration of carbon tetrachloride significantly prevented the increased serum enzymatic activities of alanine and aspartate aminotransferase in a dose-dependent manner. (omitted)

• 생명과학회지
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• 제20권5호
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• pp.782-788
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• 2010

표고버섯균사체 고체배양물 분말과 액체배양물 분말을 2:1 (w/w)로 혼합한 시료 (LED로 명명)가 $CCl_4$로 유발된 숫컷 쥐의 간독성을 보호하는 효과에 대해 연구하였다. Vehicle 처리군(0.2 ml 증류수), Control 처리군(0.2 ml 증류수), LED 처리군(LED 100, 200, 300, 400 mg/kg BW을 0.2 ml 증류수에 각각 혼합), 그리고 Silymarin 처리군(200 mg/kg을 0.2 ml 증류수에 용해)의 각 처리군(군당 여섯 마리)에 매일 2주간 투여한 다음, Vehicle 처리군을 제외한 모든 군에 $CCl_4$ ($CCl_4$:corn oil, 1:1 v/v; 0.5 ml/kg BW)를 복부에 주사하였다. 하루가 지난 후에 생화학적 지표성분을 분석하기 위해 혈액과 간 시료를 채취하였다. 모든 LED 처리에 의해 간 SOD, catalase, 그리고 GSH peroxidase의 활성이 증가하였으며, TBARS, tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-$1{\beta}$ (IL-$1{\beta}$) 그리고 interleukin-6 (IL-6)가 감소하였다. 그 결과, 혈청중의 GOT, GPT, 그리고 LDH의 활성이 감소하였다. LED의 간 기능 보호효과가 가장 효과적으로 나타난 처리농도는 200 mg/kg BW였다. 이러한 결과로 보아 LED는 $CCl_4$로 유발된 SD 쥐의 간 손상을 보호하며, 이는 LED의 항산화능과 cytokine의 감소에 의한 것이다. 따라서, LED는 사람의 간독성보호를 위해 효과적으로 사용할 수 있을 것으로 생각된다.

• 한국식품위생안전성학회지
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• 제26권3호
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• pp.235-241
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• 2011

아직 분명히 규명되지 않은 부분이 많지만, 신선초가 다양한 측면에서 건강증진 효과가 있다고 믿어져 왔고 따라서 일반인들이 지금까지 사용해 오고 있다. 이 연구에서는 신선초의 메타놀 추출물을 투여한 후, 전형적인 간독성 유발물질인 갈락토사민과 사염화탄소를 투여한 랫드에서 일반적 간독성지표와 지질대사능 지표를 측정함으로써 간장 보호작용이 있는지 시험하였다. 신선초를 독성유발전 7일간 매일 1회씩 200 및 500mg/kg의 용량으로 경구투여 하고, 간독성물질을 복강내로 투여 한 후 독성유발 24시간에 혈장과 간장조직에 대한 분석을 수행하였다. 시험한 두 용량(200 및 500 mg/kg)은 갈락토사민에 의해 유발된 지질과 산화물 증가, 혈장 AST 및 ALT 활성의 증가를 감소시켰다. 또한 신선초 500mg/kg은 갈락토사민이 유발한 혈장 중성지질, 총 콜레스테롤 및 저밀도지단백 콜레스테롤의 농도 증가현상을 감소시켰다. 갈락토사민에 의해 유발된 독성에 대한 효과와는 달리, 사염화탄소로 유발된 AST, ALT 및 과산화지질의 증가현상이 신선초 전투여에 의해 더욱 증가하였다. 이 결과는 갈락토사민에 의한 독성을 신선초 전투여가 감소시킬 수 있지만, 반대로 사염화탄소 유발 독성은 더 악화시킴을 의미한다. 신선초의 사염화탄소 유발 간독성 증강효과에 대한 기전을 이해하기 위해 신선초를 투여한 랫드의 간장내 aninline hydroxyiase의 활성을 측정하였다. 그 결과 사염화탄소가 간독성을 발휘하는 데에 필요한 조건인 대사체로의 변환을 매개하는 이 효소의 간장내 활성이 증가함을 관찰하였다. 종합적으로 신선초가 간장보호효과를 발휘하지만 간독성이 어떤 독소에 의해 유발되었느냐에 따라 향상 보호효과가 있지는 않음을 의미한다.

• 대한임상독성학회지
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• 제17권2호
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• pp.58-65
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• 2019

Purpose: Alpha-amanitin induces potent oxidative stress and apoptosis, and may play a significant role in the pathogenesis of hepatotoxicity. This study examined the mechanisms of α-amanitin-induced apoptosis in vitro, and whether green tea extract (GTE) offers protection against hepatic damage caused by α-amanitin (AMA) induced apoptosis in vivo. Methods: The effects of GTE and SIL on the cell viability of cultured murine hepatocytes induced by AMA were evaluated using an MTT assay. Apoptosis was assessed by an analysis of DNA fragmentation and caspase-3. In the in vivo protocol, mice were divided into the following four groups: control group (0.9% saline injection), AMA group (α-amanitin 0.6 mg/kg), AMA+SIL group (α-amanitin and silibinin 50 mg/kg), and AMA+GTE group (α-amanitin and green tea extract 25 mg/kg). After 48 hours of treatment, the hepatic aminotransferase and the extent of hepatonecrosis of each subject was evaluated. Results: In the hepatocytes exposed to AMA and the tested antidotes, the cell viability was significantly lower than the AMA only group. An analysis of DNA fragmentation showed distinctive cleavage of hepatocyte nuclear DNA in the cells exposed to AMA. In addition, the AMA and GTE or SIL groups showed more relief of the cleavage of the nuclear DNA ladder. Similarly, values of caspase-3 in the AMA+GTE and AMA+SIL groups were significantly lower than in the AMA group. The serum AST and ALT levels were significantly higher in the AMA group than in the control and significantly lower in the AMA+GTE group. In addition, AMA+GTE induced a significant decrease in hepatonecrosis compared to the controls when a histologic grading scale was used. Conclusion: GTE is effective against AMA-induced hepatotoxicity with its apoptosis regulatory properties under in vitro and in vivo conditions.

• 동의생리병리학회지
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• 제22권6호
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• pp.1525-1531
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• 2008

Acer tegmentosum Max which is one of the specialized wildness medicinal herbs in gangwon province, has been widely used for hepatitis, liver cirrhosis, hepatic cancer, leukemia, diabetes mellitus, renal necrosis and edema, etc. In this study, the antioxidative and hepatoprotective effects of in vitro and in vivo were investigated in order to evaluate the possibility as hepatoprotective agents. Oral administration of methanol and butanol extact of Acer tegmentosum Max to d-galactosamine (D-GaIN) induced experimental liver injured rats was significantly reduced activities of marker enzymes(AST, ALT) and LDH activity in serum. Salidroside(Sal) isolated from the BuOH extract of Acer termentosum Max potenty showed the scavenzing effect on DPPH and inhibitory effect on lipid peroxidation. And significantly decrcease of MDA level in liver and activities of SOD GSH-Px and catalase were significantly improved by the treatment of Sal. Results of this study revealed that Sal could afford a significant protection in the alleviation of D-GaIN-induced hepatocellular injury.