• Journal of Chest Surgery
• /
• 제13권2호
• /
• pp.85-91
• /
• 1980

Extracorporeal circulation by hemodilution technique has been currently used with its clinical safety and good peripheral tissue perfusion in open heart surgery. There is no doubt that $O_{2}$ carrying capacity of the blood is disturbed by decreased hemoglobin level resulting from hemodilution of the circulating blood. From the view point of the blood gas exchange, these experimental studies were undertaken to determined the sate limit of hemodilution in the condition of cardiopulmonary bypass with a constant perfusion flow rate. Twelve adult mongrel dogs weighing 10 to 13 Kg. were anesthetized with pentobarbital and then respiration was controlled with Harvard volume respirator using room air. The cardiopulmonary by pass was performed by use of Sarns heart lung machine (console 5000, 5 head and 2 roller pumps) and Travenol pediatric bubble oxygenator. The perfusion rate during bypass was maintained at a constant rate of 80 ml/min/Kg of body weight. The ratio of oxygen gas flow to blood flow was kept in 3 to 1 constantly. International hemodilution was attained by serial blood withdrawals and immediate infusion of equal volumes of diluants composed of Ringer's lactate, 5% dextrose in water and 25% mannitol solution, proportionally 60%, 30%, and 10%. Arterial and venous blood samples were obtained between 15 and 20 minutes following each hemodilution. Hematocrits and hemoglobin values, $PO_{2}$, $PCO_{2}$ and pH were measured. Oxygen and carbon dioxide contents oxygen consumption and carbon dioxide elimination were calculated groups according to different hematocrit values and the correlations were evaluated. Result were as follows. 1. the arterial $O_{2}$ tension and $O_{2}$ saturation were maintained at the physiological level irrespective of the hematocrit value. 2. The venous $O_{2}$ tension and $O_{2}$ saturation showed a tendency to decline with the decrease in hematocrit value and positive correlation between them (r = +0.49, r = +0.76), The mean values of venous $O_{2}$ tension and $O_{2}$ saturation, however, were not decreased when the hematocrit levels were lower than 20%. 3. The arterial $O_{2}$ content declined lineally in proportion to the fall of hematocrit level with a positive correlation between them (r = +0.95). 4. The venous $O_{2}$ contents were decreased gradually as the hematocrit value decreased with positive correlation between them ( r =+0.89). The trend of diminution of venous $O_{2}$ content, however, was became low according to progressive decrease of hematocrit level. 5. Systemic oxygen consumption was in higher range than $O_{2}$ requirement of basal metabolism when the hematocrit value was above 20%, but abruptly decreased when the hematocrit value became to below 20%. 6. The arterial $CO_{2}$ tension and $CO_{2}$ content showed trend of increasing with progressive decrease of hematocrit value but exhibited a rather broad range and there was no relationship between those value and the hematocrit value. 7. The venous $CO_{2}$ tension and $CO_{2}$ content have also no correlation with change of Ht. value but related directly to those value of arterial blood with positive correlation between them (r = +0.78, r = +0.95_. 8. A-V difference of $CO_{2}$ content and $CO_{2}$ elimination wasnot significantly influenced by Ht. value. From the results, we obtained that feasible limit in inteneional hemodilution is above the hematocrit value of 20% under the given experimental condition.

• 대한수의학회지
• /
• 제14권2호
• /
• pp.179-184
• /
• 1974

The blood picture of 85 healthy race horses in Korea was investigated. The ranges and mean values of erythrocyte, hemoglobin, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin concentration, and total white blood cell count in the blood picture were determine. The respective mean value and standard deviation and age differences were as follows: 1. The erythrobyte count was shown as range of 6.20 to $11.32{\times}10^6/mm^3$ with mean of $8.61{\pm}1.92{\times}10^6/mm^3$(SD). The leucocyte count was shown as range 5.0 to $18.0{\times}10^3/mm^3$ with mean of $8.25{\pm}1.51{\times}10^3/mm^3$(SD). There were not significant. differences in age, 2. The mean value of hemoglobin was shown $13.9{\pm}1.7g/100ml(SD)$ ranging 9.8 to 16.8g/100ml. The mean value of hematocrit was shown $40.9{\pm}3.94ml/100ml(SD)$ ranging 26 to 54. There were not significant differences in age. 3. The mean corpuscular hemoglobin was shown as range of 11.8 to 22.2pg with mean of $16.9{\pm}4.69$(SD). The mean corpuscular volume was shown as range of 34.5 to $71.3cu{\mu}$ with mean of $49.0{\pm}7.32cu{\mu}$(SD). The mean corpuscular hemoglobin concentration was shown as range of 30.6 to 39.4 g/100 ml with mean of $34.6{\pm}2.36$(SD). There were not significant differences in age. 4. The correlation among erythrocyte count, hemoglobin and hematocrit value were observed as follows: Erythrocyte count and hemoglobin (+0.328), rythrocyte count and hematocrit vague (+0.319). A linear regression equation was shown as follows: Erythrocyte count and hemoglobin (Y=0.336x+10.977), erythrocyte count and hematocrit value (Y=0.655x+35.274). 5. The high correlation between hemoglobin and hematocrit vague was observed (r= +0.836). A linear regression equation was shown: (Y=1.948x+13.895).

• The Korean Journal of Physiology
• /
• 제2권2호
• /
• pp.53-61
• /
• 1968

Maximal oxygen debt, lactate and excess lactate were measured in 13 men with low hematocrit ratio before and after maximal exercise. Maximal exercise run was performed on a treadmill and the duration of run was 2 minutes 45 seconds in each subject. Hematocrit ratio ranged between 35 and 47%, the mean being 39.8%. The following results were obtained. 1. Maximal oxygen debt expressed on basis of body weight increased as the hematocrit ratio decreased. The correlation coefficient between the two was r= -0.770. 2. The time necessary for decreasing to 50% of total maximal $O_2$ debt(half time) became longer as the hematocrit ratio decreased. In normal men the half time was about 4 minutes and at the longest it was 12 minutes in men with the lowest hematocrit ratio. 3. The lactate concentration reached its peak value after 3 minutes of recovery. Thereafter, the time course of decrease in lactate concentration coincided roughly with that of respiratory oxygen debt curve. To reach to the resting level, however, it took longer time than that of respiratory oxygen debt. 4. Resting concentrations of lactate was 1.28 mM/l, pyruvate 0.13 mM/l and L/P ratio was 9.8. Peak value of ${\Delta}L$ after exercise reached to the value of 10.4 mM/l and ${\Delta}L/P$ reached 26.0. Peak excess lactate after exercise was 6.34 mM/l. 5. The part of oxygen debt accounted for by the oxygen equivalent of excess lactate was only 38.4%. A better relationship between lactate and oxygen debt was observed and the part of oxygen debt accounted for by the oxygen equivalent of lactate was 63.3%. 6. Peak value of lactate after maximal exercise increased as the hematocrit ratio decreased. 7. Respiratory oxygen debt of 100 ml/kg was accounted for by lactate more than 60% and only 30% was by excess lactate. 8. Excess lactate was not a good index of respiratory oxygen debt.

• 한국식품과학회지
• /
• 제11권1호
• /
• pp.50-55
• /
• 1979

인삼 nectar, 인삼추출물이 함유된 orange juice, 인삼 drink 제등을 정상식이에 첨가 (555 mg Ginseng extract/kg of diet, 1,110 mg Ginseng extract/kg diet)하여 이유 직후의 Sprgue-Dawley strain 백쥐 40마리를 8마리씩 5군으로 나누어 12주간 사육하였다 12주후에 체중증가율, 사료효율, 장기의 무게, hematocrit value, serum cholesterol content, serum GOT and GPT 활성등을 측정하여 정상식이군과 비교한 결과 체중증가율, 사료효율, 장기의 무게, hematocrit value, SGOT 활성등을 모두 정상식이군에 비해서 실험군이 통계적으로 유의차를 보이지 않았으나, s-GOT 활성은 대조군에 비해 실험군이 다소 낮은 경향을 관찰할 수 있었고, s-cholesterol 농도는 대조군에 비해 다소 높은 경향이었으나 통계적인 유의차는 없었다. 백쥐 각군의 간, 신장, 비장등의 조직세포를 검사한 바 하등의 이상 조직을 발견하지 못하였다. 12주 실험이 끝난 후 체중을 측정하고 swimming test를 실시한 결과 대조군에 비해 실험군의 수영시간이 연장되었으며 V군에서는 유의성있게 연장되었다.

• 사상체질의학회지
• /
• 제3권1호
• /
• pp.151-172
• /
• 1991

Comparative blood analysis was carried out to investigate the change of blood constituent in TAE-EUM-IN group and control group. RBC, WBC, hemoglobin, hematocrit, total protein, triglyceride, phospholipid, total cholesterol, LDL-cholesterol, HDL-cholesterol, BUN, creatinine, ACTH, cortisol and prostaglandin E and $F_2{\alpha}$ were measured. And the following results were obtained: 1. In the change of blood cell, the value of hematocrit showed significant difference, while that of RBC, WBC and himoglobin didn't. 2. In the change of protein, the value of total protein showed significant difference, but that of albumin didn't. 3. In the change of lipid and cholesterol in serum, the value of triglyceride, phospholipid, total cholesterol and LDL-cholesterol indicated significant difference, while that of HDL-cholesterol indicated significant difference, while that of HDL-cholesterol didn't. 4. The value of BUN in serum represented significant difference, but that of creatinine didn't. 5. The value of cortisol in plasma showed significant difference, but that of ACTH didn't. 6. The value of prostaglandin E and $F_2{\alpha}$ in plasma showed significant difference. These results suggest that investigation on hormones and blood constituents enables objectification in differentiation of four types of physical constitution.

• 대한임상검사과학회지
• /
• 제43권4호
• /
• pp.156-160
• /
• 2011

It was confirmed that we got somewhat different results even though we performed same items with same methods from capillaries and venous blood of healthy 72 cases. Items which capillaries blood has higher value than venous blood are the numbers of erythrocyte, lymphocyte, basophil, hematocrit, MCHC and RDW. Total numbers of RBC (p<0.035), lymphocyte and basophil are shown statistically significance. Items which venous blood has higher value than capillary blood are the numbers of platelet (p<0.00) and neutrophil (p<0.01). Fallible items in clinics can be shown the numbers of RBC, platelet, lymphocyte and neutrophil because we got somewhat different results even though we performed same items with same methods from capillaries and venous blood, respectively. It is necessary to choose the clear criteria and normal value depends on clinical specimen as the number of platelets are measured with an abnormal value shown over 40% down. As normal difference according to each item and method is currently acceptable and applicable in clinics, it is considered that a new normal value depends on clinical specimen should be established and is to be useful positively in clinics.

• 한국식품영양과학회지
• /
• 제7권2호
• /
• pp.29-34
• /
• 1978

It was conducted to find out that the influence of lipids on blood cholesterol level of chicks and ducks. In this experiments, a comparative study was carried out using chicks and ducks divided into four groups with various diet for the period of four weeks. The results were as follows: 1. The mean value of hematocrit was significantly lower for the chicks fed sesame oil and soybean oil than the chicks fed duck meat. The value of hematocrit was higher for the ducks fed sesame oil, soybean oil and duck meat than for the control group. 2. Blood glucose level was higher for the ducks group than the chicks group. It was lower blood glucose level for the chicks and ducks fed duck meat than any other groups. 3. Blood cholesterol level was lower for the chicks fed sesame oil, soybean oil, and duck meat than for those fed the basal diet(control group). In the ducks group, blood cholesterol level was high. But in the liver organ, it was lower blood cholesterol level for the chicks and ducks fed sesame oil, soybean oil and duck meat, respectively than for those fed the basal diet(control group). 4. Phospholipid of the chicks which were control groups was lower value than for those fed sesame oil and duck meat fed, the value of phospholipid was higher value.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권1호
• /
• pp.118-121
• /
• 1997

A total of 72 Black Bengal goats of 2.5 to 3.5 and 4.0 to 6.0 years old were used in this study. Equal number of animals were included in Fasciola gigantica infected and non-infected control groups. For each age and treatment groups 18 blood samples were collected in glass vials contained EDTA anticoagulant in summer and winter seasons before the animals were slaughtered at abottoir. Packed cell volume (PCV) was determined using microhematocrit and hemoglobin (Hb) concentration by cyanmethemoglobin methods. The PCV of F. gigantica infected animals were significantly lower than the non-infected animals. The average PCV values obtained were 26.60 and 32.20% for F. gigantica infected and non-infected animals, respectively. The Hb values of infected animals were significantly lower than the non-infected animals. The average Hb values obtained were 9.17 and 10.51 gm% for F. gigantica infected and non-infected goats, respectively. There was no significant effect of age and season on the values of PCV and Hb of infected and non-infected animals.

• 대한예방한의학회지
• /
• 제4권1호
• /
• pp.17-31
• /
• 2000

In order to investigate the effects of emitted-qi therapy(EQT) and cold water therapy(CWT) on the inhibition of the blood loss, the protein loss and the electrolyte loss in burned rats. The white blood cell, hematocrit and hemoglobin values in blood, and the total protein, albumin, globulin, $Na^+,\;Cl^-,\;K^+\;and\;Ca^{2+}$ values in serum were measured. The results were obtained as follows; 1. White blood cell count in blood was significantly increased in CWT group compared with control group. 2. Hematocrit value in bolld was significantly increase in EQT and CWT group compared with control group. 3. Hemoglobin value in blood was significantly increased in CWT group compared with control group. 4. Total protein value in serum was significantly increased in CWT group compared with control group. 5. Albumin value in serum was significantly increased in EQT and CWT group compared with control group. 6. Globulin value in serum tended to be increase in EQT and CWT group compared with control group, but it was not significant. 7. A/G ratio in serum was significantly increased in EQT and CWT group compared with contort group. 8. $Na^+$, value in serum was significantly increased in EQT and CWT group compared with ontrol group. 9. $K^+$, value in serum tended to be increased in EQT group compared with control group, but it was not significant. 10. $Cl^-$ value in serum was significantly increased in EQT and CWT group compared with control group. 11. $Ca^{2+}$ value in serum tended to be increased in EQT and CWT compared with control group, but it was not significant. According to the above results, the EQT and CWT inhibited the blood loss, the protein loss and the electrolyte loss. Therefore, it is considered that the EQT and CWT can be applied to the burn therapy.

• 약학회지
• /
• 제47권6호
• /
• pp.422-431
• /
• 2003

The use of recombinant human erythropoietin (rhEPO), a stimulator of erythropoiesis, banned in sports because of the medical risk associated with thrombosis. Due to analytical difficulties to differentiate between natural human EPO (hEPO) and rhEPO, blood parameters of erythropoiesis such as contents of hemoglobin (cut-off value <17.5 g/d l for man, and < 16.0 g/dl for women), hematocrit and reticulocytes (cut-off value <2.0%) were measured to focus the misuse of rhEPO. We conducted anti-doping test for 122 blood samples of the World Cup athletes. The mean values of key parameters are as follows; 14.5$\pm$1.0 g/dl for hemoglobin, 41.7$\pm$2.8% for hematocrit, and 1.3$\pm$0.4% for reticulocyte. Blood sample was found to be stable up to 8 hours for the reticulocyte measurement. In addition, the soluble transferrin receptor and ferritin levels were measured by immunoassay methods using plasma samples (n=28) in which the mean value was 0.8$\pm$0.5 $\mu\textrm{g}$/$m\ell$ and 54.6$\pm$33.7 ng/$m\ell$, respectively. The results indicate that all samples tested were negative for the blood parameters of indirect anti-doping test for hEPO misuse. The statistical evaluation suggest that several other parameters such as red blood cell, mean corpuscular hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin and white blood cell could be considered as factors influencing hEPO function in addition to five parameters mentioned.