• 대한예방한의학회지
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• 제4권2호
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• pp.57-71
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• 2000

BACGROUND: To examine effect of omahwan(烏麻丸)-赤何首烏(Polygonum multiflorum THUNB.), 白何首烏(Cynanchum wilfordii H.), 黑芝流(Sesamum indicum L.)-on the aged human immune system a series of humoral immunological parameters was compared in aged female (age 60-70 years) before and after administration of omahwan(烏麻丸). METHODS: Peripheral blood was obtained from fifteen healthy young (age 20-29, mean=66.3) female volunteers. B cell, T cell(T cells, T helper, and T suppressor/ cytotoxic) subsets was examined with specific monoclonal antibodies and isotype controls, using dural color flow cytometer IL-2 was examined with ELISA kit RESULT: By comparing the immune characteristics of the younger and elder groups the total ratio of T-cell, CD8 T-cell and quantify of IL-2 was significantly lower while CD4/CDB ratio was considerably greater in the elder group. ( p(0.05, student t-test) After giving Omahwan for 30 days to the elder group, by comparing the ratio before and after Prescription the total T cell and CD8 T cell ratio was considerably greater after prescription (p(0.05, Paired t-test) The quantity of IL-2 tended to increase after prescription but has no statistical meaning. CONCLUSION: The results indicate that administration of Omahwan(烏麻丸) differentially affects various aspects of the immune system in aged human.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.85-89
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• 2015

This peptide has antibacterial activity against several Gram-positive and Gram-negative bacteria. Bombyx mori cecropinB1(BmCecB1) is antimicrobial peptides from Bombyx mori and belongs to cecropin family. Antimicrobial peptides are important components of the innate immune systems in all living organism. To produce the BmCecB1 antimicrobial peptide, we constructed transgenic silkworm that expressed BmCecB1 gene under the control BmA3 promoter using piggyBac vector. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. Mixtures of the donor vector and helper vector were micro-injected into 600 eggs of bivoltin silkworms, Baegokjam. In total, 49 larvae (G0) were hatched and allowed to develop into moths. The resulting G1 generation consisted of 22 broods, and we selected 2 broods containing at least 1 EGFP-positive embryo. The rate of successful transgenesis for the G1 broods was 9%. We identified 9 EGFP-positive G1 moths and these were backcrossed with wild-type moths. With the aim of identifying a BmCecB1 as antimicrobial peptide, we investigated the Radical diffusion Assay (RDA) and then demonstrated that BmCecB1 possesses high antibacterial activities against Gram-negative bacteria.

• 한국도서관정보학회지
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• 제36권1호
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• pp.47-69
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• 2005

구전설화에 바탕을 두고 있는 전래동화는 다양한 이본이 존재하는데, 어린이들을 위해 어떤 책을 선택하는 것이 좋을지, 즉 어느 책의 본문이 가장 좋을지가 늘 의문이었다. 이 연구는 이 의문을 해결하기 위하여 시도된 것으로 원문서지학의 연구방법으로 문헌을 비평하였다. 널리 알려진 전래동화 <해님과 달님>의 이본 6편을 대상으로 줄거리 파악을 통해 구조를 분석하고 이를 토대로 제목, 배경, 등장인물, 문체, 기능을 비교 분석하였다. 그 결과 6편의 이본 가운데 어효선본의 본문이 비교적 우수하다고 판단되었다. 어효선본에서 조력자인 옥황상제를 하느님으로 바꾸고, 문체를 구어체로 바꾸며, 해와 달이 된 오누이가 서로 바꾸는 마지막 장면을 삭제한다면 이상적인 원문이 될 것이다. 이러한 원문서지학적인 연구가 축적되고, 이러한 역량이 갖추어질 때 비로소 사서가 내용, 즉 컨텐츠에 대해서 발언할 수 있게 될 것이다.

• 대한한의학방제학회지
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• 제8권1호
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• pp.257-279
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• 2000

Allergic contact dermatitis is a common skin disease resulting from specific immunologic to topically applied various allergen. After Dinitrochlorobenzene (DNCB) secondary sensitization, the ICR mice administered Rhemanniae radix extract (RRE) was observed to ascerstain the effect of RRE on allergic contact dermatitis. Purpose of this study was to investigate contact hypersensitivity assay, abdominal skin morphologic changes. Including mast cells and cell-surface glycoconjugates. The change of interleukin 2 (IL-2) receptor (CD25R). ICAM in abdominal skin, lymph node of inguinal region, and electro microscope-morphologic changes of abdominal skin. The results of this study were as follows: 1. The contact hypersensitivity assay, the ear swelling in the RRE had lesser probability than in the ACD Group. 2. In the general morphologic change of skin, hyperplasia of keratinocytes, distribution of vasculogenesis and epidermal lymphocytes infiltration were decreased in the RRE group compared with the ACD group. In epidermal basal layer and prickle layer, cell damage was decreased in the RRE group compared with the group painted with ACD. 3. MasT-cell in dermis was decreased in the RRE group compared with the group painted with DNCB. 4. Distribution of interlukin-2 Receptior positive cell and ICAM positive cell in dermis was decreased in the RRE Group compared with the ACD group. 5. Distribution of helper T-lymphocyte and cytotoxic T-lymphocite in inguinal nodes was decreased in the RRE group, and was observed well in paracortical area and cortical cord. 6. Distribution of apoptotic cell was appeared in the RRE group compared with the ACD group, in skin, dermis. in inguinal nodes, paracortical area observed well. With above results, the restarint of immunosuppression occuring in Allergic contact dermatitis is resulted in the slow progress the effect of Allergic contact dermatitis, and it is thought that ithis fact has a series of relation with apoptosis.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.463-469
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• 2002

Rotaviruses are the world-wide leading causative agents of severe dehydrating gastroenteritis in young children and animals. The outer capsid glycoprotein VP7 and inner capsid glycoprotein VP6 of rotaviruses are highly antigenic and immunogenic. An SFV-based expression system has recently emerged as a useful tool for heterologous protein production in mammalian cells, exhibiting a much more efficient performance compared to other gene expression systems. Accordingly, the current study adopted an SFV-based expression system to express the VP7 of a group A human rotavirus from a Korean isolate, and the VP6 of a group B bovine rotavirus from a Korean isolate, in mammalian cells. The genes of the VP6 and VP7 were inserted into the SFV expression vector pSFV-1. The RNA was transcribed in vitro from pSFV-VP6 and pSFV-VP7 using SP6 polymerase. Each RNA was then electroporated into BHK-21 cells along with pSFV-helper RNA containing the structural protein gene without the packaging signal. The expression of VP6 and VP7 in the cytoplasm was then detected by immunocytochemistry. The recombinant virus was harvested by ultracentrifugation and examined under electron microscopy. After infecting BHK-21 cells with the defective viruses, the expressed proteins were separated by SDS-PAGE and analyzed by a Western blot. The results indicate that an SFV-based expression system fur the VP6 and VP7 of rotaviruses is an efficient tool for developing a diagnostic kit and/or preventive vaccine.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.490-497
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• 2004

Although the E. coli heat-labile enterotoxin B subunit (LTB) is known to be a potent mucosal adjuvant towards co-administrated unrelated antigens and immunoregulator in T-helper 1-type-mediated autoimmune diseases, a more efficient and useful LTB is still required for prospective vaccine adjuvants. To determine whether a novel chimeric LTB subunit would produce an enhanced mucosal adjuvant activity and immune response, a number of LTB subunits were genetically fused with chimeric proteins using the epitope genes of the envelope glycoprotein E2 (gp51-54) from the classical swine fever virus (CSFV). It was found that the total serum immunoglobulin (Ig) levels of BALB/c mice orally immunized with chimeric proteins containing an N-terminal linked LTB subunit (LE1, LE2, and LE3) were higher than those of mice immunized with LTB, E2 epitope, and chimeric proteins that contained a C-terminal linked LTB subunit. In particular, immunization with LE1 markedly increased both the total serum Ig and fecal IgA level compared to immunization with LTB or the E2 epitope. Accordingly, the current results demonstrated that the LTB subunit in a chimeric protein exhibited a strong mucosal adjuvant effect as a carrier molecule, while the chimeric protein containing the LTB subunit stimulated the mucosal immune system by mediating the induction of antigen-specific serum Ig and mucosal IgA. Consequently, an LE1-mediated mucosal response may contribute to the development of effective antidiarrhea vaccine adjuvants.

• Journal of Microbiology and Biotechnology
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• 제26권3호
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• pp.469-476
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• 2016

This study was undertaken to evaluate the immunomodulatory effect of dead nano-sized Lactobacillus plantarum (nLp) in RAW 264.7 cells and murine primary splenocytes. nLp is a dead, shrunken, processed form of L. plantarum nF1 isolated from kimchi (a traditional Korean fermented cabbage) and is less than 1 μm in size. It was found that nLp treatment stimulated nitric oxide (NO) production more in RAW 264.7 macrophages than pure live L. plantarum (pLp), and that the stimulatory properties were probably largely derived from its cell wall. In addition, nLp induced murine splenocyte proliferation more so than pLp; in particular, a high dose of nLp (1.0 × 10¹¹ CFU/ml) stimulated proliferation as much as lipopolysaccharide at 2 μg/ml. Moreover, according to our cytokine profile results in splenocytes, nLp treatment promoted Th1 (TNF-α, IL-12 p70) responses rather than Th2 (IL-4, IL-5) responses and also increased Th17 (IL-6, IL-17A) responses. Thus, nLp stimulated NO release in RAW 264.7 cells and induced splenocyte proliferation more so than pLp and stimulated Th1 and Th17 cytokine production. These findings suggested that dead nLp has potential use as a functional food ingredient to improve the immune response, and especially as a means of inducing Th1/Th17 immune responses.

• 기술혁신학회지
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• 제19권3호
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• pp.572-603
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• 2016

본 연구는 일반적인 현상으로서의 표준정책을 과학기술 연구를 수행하는 과학자들의 입장에서 이해하고, 과학자들의 인식 및 행태 속에 투영 된 표준정책을 질적으로 탐구한다. (1) 융합 R&D 부문에서 표준정책의 방향성과 (2) 그러한 표준화 방향성을 위해 정부의 역할과 융합 R&D 연구자들의 역할을 탐색하였다. 15명의 융합 R&D 과학자들과의 인터뷰 분석 결과, 융합 R&D에서의 표준화는 분명 일반 R&D에서의 그것과는 차이가 있었고, 융합 R&D현장에서의 표준화 고민이 복합적이고 다양하다는 점을 엿볼 수 있었다. 융합 R&D와 표준의 관계에서 융합기술결과에 대한 낮은 예측가능성으로 인해, 기술의 경쟁력 및 객관성을 확보하기 위하여 R&D를 일정기간 수행한 다음, 기술의 표준화 업무가 진행되는 것이 일반적인 융합 R&D와 표준의 관계로 해석해 볼 수 있었다. 우리나라의 국가표준정책이 취해야 할 정책 방향성은 "민간(시장) 주도의 표준 리드"로 압축된다. 정부는 공공부문에 대한 것은 정부 주도의 표준화를 진행하고 민간 부문에 대한 것은 최대한 민간 자율에 맡겨 자연스럽게 글로벌 표준을 선도하도록 환경을 조성해 주는 것이 필요하다. 이렇듯, 국가표준정책에서 정부의 역할은 "조력자"의 역할로 요약된다.

• 한국임상수의학회지
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• 제16권2호
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• pp.454-462
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• 1999

Corticosteroids have long been used for anti-inflammatory, anti-rheumatoid and other purposes in hospital. These effects may be due to inhibit immune reaction. So the animal given corticosteroids was more susceptible to infection because of immunosuppressive effect of corticosteroids. The purpose of this study was to investigate the effects of prednisolone on the lymphocyte subset in the spleen, immunoglobulin in serum, spleen weight, thymus weight and total WBC in peripheral blood. Mice were randomized into 3 groups. Each group has 24 mice. The small dosage group were given by 4 mg/kg/day of prednisolone for 4 days and the large dosage group were given by 8 mg/kg/day respectively. Prednisolone was suspended in saline and orally administered. Mice in control group were given saline alone. Eight mice in each group were sacrificed every week after administration of predisolone. The weight of thymus and spleen were mesured immediately. Lymphocytes were taken from spleen and these cells were analysed by flow cytometry. Also the concentration of total immunoglobulins in serum were assayed by enzyme-linked immunosorbant assay (ELISA). T cell, T-helper cell and T-cytotoxic cell were all significantly (P<0.05) decreased at 1 week after administration of predisolone and at 2 weeks they recovered similarly to that of control. Population of B cell showed various distribution. The concentration of total immunoglobulins in serum was not changed significantly. The weight ratio of spleen to body decreased significantly (P＜0.05) during predisolone administration but increased at 1 week later, Eventually the weight ratio was recovered to that of control at 2 weeks. The weight ratio of thymus to body decreased significantly (P<0.05) by prednisolone and recovered gradually up to normal ratio 2 weeks later.

• 한국학교ㆍ지역보건교육학회지
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• 제2권2호
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• pp.1-22
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• 2001

The purpose of this study was to evaluate the effect of moderate physical exercise program on a number of immune parameters in middle school boys($15.07{\pm}0.39$ yrs). 14 volunteers were included in this physical exercise program. They were randomly assigned to an experiment(7) and a control(7) group. Measurements were taken before program, after 4 weeks and 8 weeks of aerobic exercise for immune parameters such as number of circulating leukocytes, concentration of WEC subsets, lymphocyte subsets, immunoglobulins, complements, and number of blood adipose components. Aerobic exercise consisted of track running at a work intensity of 65% HRmax, $45{\sim}55$ min per day, 3 times per week, and for 8 weeks. The results are as follows; 1. There were no significant changes between and within groups in the number of circulating leukocytes and concentration of WEC subsets. 2. There were significant(p<.05) changes in concentration of B-cell between groups, and T-cell, helper T-cell, and B-cell within experimental group. 3. There were no significant changes between and within groups in concentration of complements. 4. There were significant(p<.05) changes in concentration of IgG between groups and within experimental group. 5. There were significant(p<.05) changes in concentration of TG between groups, and TG, HDL-C and LDL-C within experimental group. In conclusion, the moderate exercise training for 8 weeks can be beneficial on immune function and decrease the concentration of flood adipose components in adolescents.