• The Plant Pathology Journal
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• 제39권6호
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• pp.575-583
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• 2023

Fusarium root rot is an increasingly severe problem in soybean cultivation. Although several Fusarium species have been reported to infect soybean roots in Heilongjiang province, their frequency and aggressiveness have not been systematically quantified in the region. This study aimed to investigate the diversity and distribution of Fusarium species that cause soybean root rot in Heilongjiang province over two years. A total of 485 isolates belonging to nine Fusarium species were identified, with F. oxysporum and F. solani being the most prevalent. Pot experiments were conducted to examine the relative aggressiveness of different Fusarium species on soybean roots, revealing that F. oxysporum and F. solani were the most aggressive pathogens, causing the most severe root rot symptoms. The study also assessed the susceptibility of different soybean cultivars to Fusarium root rot caused by F. oxysporum and F. solani. The results indicated that the soybean cultivar DN51 exhibited the most resistance to both pathogens, indicating that it may possess genetic traits that make it less susceptible to Fusarium root rot. These findings provide valuable insights into the diversity and distribution of Fusarium species that cause soybean root rot and could facilitate the development of effective management strategies for this disease.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.228-237
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• 1996

On the basis of a brief introduction of the mechanized dry farming in Heilongjiang Province, the author states the developing process from the combination of single technics of farm machinery and agronomy to the technical assembly of high yield cultivation technics and its mathematical expression. According to the main temperature accumulated zones, 5 typical comprehensive technical assembly models for the mechanized cultivation technics and their agricultural machinery systems have been listed. They are, the Heihe " 261" wheat and soybean model : the Yi'an big ridge double row film mulching corn model : the Yongchang high yield mechanized soybean and other grain crops six year rotation model for Keshan state farms. The author conclude that the application of mechanized high yield cultivation technical assembly is the key point to transform the Heilongjiang province from big agriculture to strong agriculture, we have to take " high yield , high quality , high efficiency , s stain -ability and earning foreign currency" as the general target and carry out the corresponding policy and measures for the further development of agricuture.

• Journal of Veterinary Science
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• 제23권6호
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• pp.90.01-90.13
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• 2022

Background: Insulin regulates glucose homeostasis and has important effects on metabolism, cell growth, and differentiation. Depending on the cell type and physiological context, insulin signal has specific pathways and biological outcomes in different tissues and cells. For studying the signal pathway of insulin on glycolipid metabolism in porcine embryonic fibroblast (PEF), we used high-throughput sequencing to monitor gene expression patterns regulated by insulin. Objectives: The goal of our research was to see how insulin affected glucose and lipid metabolism in PEFs. Methods: We cultured the PEFs with the addition of insulin and sampled them at 0, 48, and 72 h for RNA-Seq analysis in triplicate for each time point. Results: At 48 and 72 h, 801 and 1,176 genes were differentially expressed, respectively. Of these, 272 up-regulated genes and 264 down-regulated genes were common to both time points. Gene Ontology analysis was used to annotate the functions of the differentially expressed genes (DEGs), the biological processes related to lipid metabolism and cell cycle were dominant. And the DEGs were significantly enriched in interleukin-17 signaling pathway, phosphatidylinositol-3-kinase-protein kinase B signaling pathway, pyruvate metabolism, and others pathways related to lipid metabolism by Kyoto Encyclopedia of Genes and Genomes enrichment analysis. Conclusions: These results elucidate the transcriptomic response to insulin in PEF. The genes and pathways involved in the transcriptome mechanisms provide useful information for further research into the complicated molecular processes of insulin in PEF.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3465-3469
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• 2012

Aim: Platinum agents have shown to be effective in the treatment of colorectal cancer. We assessed whether single nucleotide polymorphisms (SNPs) in GSTP1, ERCC1 Asn118Asn and ERCC2 Lys751Gln might predict the overall survival in patients receiving oxaliplatin-based chemotherapy in a Chinese population. Methods: SNPs of GSTP1, ERCC1 Asn118Asn and ERCC2 Lys751Gln in 335 colorectal cancer patients were assessed using TaqMan nuclease assays. Results: At the time of final analysis on Nov. 2011, the median follow-up period was 37.7 months (range from 1 to 60 months). A total of 229 patients died during follow-up. Our study showed GSTP1 Val/Val (HR=0.44, 95% CI=0.18-0.98), ERCC1 C/C (HR=0.20, 95% CI=0.10-0.79) and ERCC2 G/G (HR=0.48, 95% CI=0.19-0.97) to be significantly associated with better survival of colorectal cancer. GSTP1 Val/Val, ERCC1 C/C and ERCC2 G/G were also related to longer survival among patients with colon cancer, with HRs (95% CIs) of 0.41 (0.16-0.91), 0.16 (0.09-0.74) and 0.34 (0.16-0.91), respectively. Conclusion: GSTP1, GSTP1, ERCC1 Asn118Asn and ERCC2 Lys751Gln genotyping might facilitate tailored oxaliplatin-based chemotherapy for colorectal cancer patients.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10613-10619
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• 2015

Background: Both alcohol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, the dried root tuber of which is named Baifuzi in Chinese, have been used for folklore treatment of cancer in Northeast of China. However, little is known about which is most suitable to the cancer therapy. Materials and Methods: Serum pharmacology and MTT assays were adopted to detect the effects of ethanol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, prepared by heat reflux methods, on proliferation of different cancer cells. Results: Cancer cells treated with medium supplemented with 10%, 20%, 40% serum(v/v) containing ethanol extract had a decline in viability, with inhibition rates of 7.69%, 21.8%, 41.9% in MCF-7 cells, 42.8%, 48.1%, 51.8% in SGC-7901 cells, 44.1%, 49.2%, 53.7% in SMMC-7721 cells, 6.8%, 15.2%, 39.8% in HepG2 cells, 7.57%, 16.3%, 36.2% in HeLa cells, 6.24%, 12.5%, 27.4% in A549 cells, and 7.20%, 17.5%, 31.3% in MDA-MB-231 cells, respectively. Viability in the aqueous extract groups was no different with that of controls. Conclusions: An ethanol extract of Sauromatum giganteum(Engl.) Cusimano and Hett inhibited the proliferation of SMMC-7721, SGC-7901 and MCF-7 cells, which supports the use of alcoholic but not aqueous extracts for control of sensive cancers, which might include hepatocarcinoma, gastric cancer and breast cancer.

• The Korean Journal of Ecology
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• 제23권4호
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• pp.339-345
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• 2000

Heilongjiang Province has set up 104 nature reserves, with a total area of 2,641,700 ha, or 5.88% of the total area of the province. These constitute a network of nature reserves comprising most important types of nature reserves. and play an active role for improving the protection of the ecological environment and for the continuous economic and social development of the province. But in the management of these nature reserves, there are still some problems with people's idea, with educating the public, and with capital investment. These problems should be treated seriously. To preserve our natural heritage and biodiversity and to promote the sustainable development of our society and economy, it is projected that by the year 2000. the number of nature reserves in Heilongjiang is to reach 109, covering 6.17% of the total land area of the province. This percentage will be further increased to 8.62% by 2010, at which time the network of nature reserves is to have a proper distribution and comprehend all the important types of nature reserves. This will ensure the healthy development of the cause of nature conservation. with systematic planning, active protection. and sustainable use, so that ecological and social benefits can be developed hand in hand with economic benefits.

• Journal of Veterinary Science
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• 제23권2호
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• pp.40.1-40.13
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• 2022

Background: Somatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency. Objectives: This study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs. Methods: The viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The in vitro developmental ability of SCNT embryos was evaluated by the blastocyst rate and the expression levels of the core pluripotency factor. Blastocyst cell apoptosis was examined using a terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. The in vivo developmental ability of SCNT embryos was evaluated after embryo transfer. Results: Most sow urine-derived cells were viable and could be cultured and propagated easily. On the other hand, most of the somatic cells isolated from the boar urine exhibited poor cellular activity. The in vitro development efficiency between the embryos produced by SCNT using porcine embryonic fibroblasts (PEFs) and urine-derived cells were similar. Moreover, The H3K9me3 in SCNT embryos produced from sow urine-derived cells and PEFs at the four-cell stage showed similar intensity. The levels of Oct4, Nanog, and Sox2 expression in blastocysts were similar in the two groups. Furthermore, there is a similar apoptotic level of cloned embryos produced by the two types of cells. Finally, the full-term development ability of the cloned embryos was evaluated, and the cloned fetuses from the urine-derived cells showed absorption. Conclusions: Sow urine-derived cells could be used to produce SCNT embryos.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6437-6441
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• 2014

Background: Oridonin isolated from Rabdosia rubescens, a plant used to treat cancer in Chinese folk medicine, is one of the most important antitumor active ingredients. Previous studies have shown that oridonin has antitumor activities in vivo and in vitro, but little is known about cell cycle effects of oridonin in gastric cancer. Materials and Methods: MTT assay was adopted to detect the proliferation inhibition of SGC-7901 cells, the cell cycle was assessed by flow cytometry and protein expression by Western blotting. Results: Oridonin could inhibit SGC-7901 cell proliferation, the $IC_{50}$ being $15.6{\mu}M$, and blocked SGC-7901 cell cycling in the $G_2/M$ phase. The agent also decreased the protein expression of cyclinB1 and CDK1. Conclusions: Oridonin may inhibit SGC-7901 growth and block the cells in the $G_2/M$ phase by decreasing Cdk1 and cyclinB1 proteins.

• Journal of Korean Neurosurgical Society
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• 제59권4호
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• pp.334-340
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• 2016

Objective : The aim of our study was to evaluate the neuroprotective functions of the combination therapy using methylprednisolone (MP) and tranilast (TR) after spinal cord injury (SCI) in adult rats. Methods : Spinal cord compression injury model was achieved using Yasargil aneurysm clip. Rats were divided into control group, MP group, TR group, and combination therapy group using TR and MP. Rat models were assessed for locomotor functional recovery using Basso, Beattie, and Bresnahan (BBB) score, spinal cord water content and myeloperoxidase (MPO) activity 24 hours post SCI, haematoxylin and eosin staining and glial fibrillary acid protein (GFAP) staining at 7 and 14 days post SCI. Results : The spinal cord water content and MPO activity in the combination therapy group was significantly lower than the control group and the individual therapy groups p<0.05. The combination therapy group had significantly higher BBB scores than control group and individual therapy groups (p<0.05). At one week after SCI, GFAP expression in the combination group was significantly lower than the control group (p<0.05) but there was no significant difference compared to the individual therapy groups (p>0.05). At 2 weeks after SCI there was a slight decrease in GFAP expression compared to the first week but the difference was not statistically significant (p>0.05), GFAP expression between the groups was not statistically significant p>0.05. Conclusion : Combining MP and TR is therapeutically more effective in improving functional recovery, inhibiting inflammation and glial scar formation after acute SCI.

• 동양고전연구
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• 제57호
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• pp.301-330
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• 2014

문헌기록에 숙신이란 명칭이 처음 확인되는 것은 "사기(史記)"와 "춘추좌씨전(春秋左氏傳)"이다. 그러나 숙신이 어떤 계통의 종족이고, 언제 처음 나타났으며, 주 활동지역이 어디이고, 어떻게 생활하였는지에 대해서는 구체적인 기록은 남아있지 않아 그 실체를 확인하는데 어려움이 있다. 그동안 숙신의 활동지역에 대해서는 다양한 견해가 제기되었으나, 중국의 경우 흑룡강성 동부지역을 숙신의 활동지역으로 보는 "숙신동북설"이 학계의 정설로 받아들여지고 있다. 최근 국내에서 "숙신동북설"에 대한 비판적 연구가 이루어졌으나, 문헌비판에만 그치고 있어 일정한 한계를 지니고 있다. 본고는 이러한 문헌사적 연구성과를 토대로 중국측이 주장하는 숙신 활동 지역의 고고학적 자료를 분석해 봄으로써 "숙신동북설"의 문제점과 허구성을 밝혀보고자 한다. 흑룡강성 동부지역의 선사시대 유적으로는 신개류유적, 소남산유적, 앵가령유적, 석회장유적, 진흥유적, 아포력유적 등이 있다. 이들 유적에서 생활한 선사인들은 어렵과 수렵 중심의 경제생활을 영위하고 있다. 다만 목단강유역의 경우 어렵과 수렵 이외에도 일부 원시 농업이 행해지고 있다. 사회구조는 남성 위주의 가부장적 씨족부락 단계에 해당되며, 복합사회 단계에는 도달하지 못하고 있다. 문헌에 중국 왕조와 숙신과의 교류 관계 기사는 요순시기와 주 무왕 시기 두 차례 보인다. 두 시기로 나누어 중원과 흑룡강지역 유적에 보이는 문화양상을 비교 분석한 결과, 양자간의 문화적 교류 가능성은 확인되지 않는다. 이는 선사시대 흑룡강성 동부지역은 중원과 교류할 수 있을 정도의 복합사회 단계로 발전하지 못하고, 가부장적인 씨족부락단계에 머무르고 있었기 때문으로 파악된다. 결과적으로 중국에서 주장하는 "숙신동북설"은 진수의 "삼국지" 저술 과정에서 발생한 오류와 현재적 시점에서 중화민족통일론에 입각해 만들어진 허구적 학설에 불과하다.