• 한국화재소방학회논문지
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• 제26권3호
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• pp.79-84
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• 2012

본 연구에서는 국내외 스프링클러헤드 감도시험 기준에 적용하고 있는 반응시간지수(RTI, Response Time Index)를 사용하여 대류항과 전도항 그리고 시간 변화량을 고려한 감열부의 열전달 현상을 해석적으로 분석하였다. 원형 실린더 형상을 갖는 감열부의 열적 특성을 분석하기 위해서 비제차 2차 편미분 형태의 에너지 방정식을 사용하여 감열부 표면의 온도가 일정하고 대칭(Symmetric)인 경계조건을 적용하여 시간 증가에 대한 감열부의 반지름 방향 온도분포를 구하였다. 그 결과 본 연구의 감열부 열적 특성에 관한 분석 기법은 스프링클러헤드의 감도시험 및 감열부의 설계를 위한 자료의 활용이 가능할 것으로 판단된다.

• Animal cells and systems
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• 제4권2호
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• pp.181-186
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• 2000

The expression of $p21^{WAF1/ClP1/SDl1}$, one of the cyclin-dependent kinase inhibitors, is regulated by a variety of transcription factors including p53 and STAT. Heat shock induces the expression of p21 in a temperature- and time-dependent manner. Although the p21 induction by heat shock has been reported to be controlled by p53, a p53-independent mechanism Is also involved. To understand the p53-independent regulation of heat shock-induced p21 expression, we searched the promoter region of p21 gene and found one or two heat shock element (HSE)-like sequences in human, rat, and mouse. Electromobility shift assay (EMSA) showed that heat shock factor (HSF) could bind to these HSE-like sequences In response to heat shock, even though to a lesser extent than to HSE. In addition, p21 promoter deletion analysis revealed that heat shock activated a p21 deletion promoter construct containing the HSE-like sequences but lacking p53-binding sites, but not a promoter construct containing neither HSE-like sequences nor the p53-responsive element. Furthermore, the p21 induction by heat shook was significantly inhibited in confluent cells in which heat shock-induced HSF activation was reduced. These results suggest that the transcriptional regulation of p21 by heat shock may be mediated through activation and binding to HSE-like sequences of HSF.

• Molecules and Cells
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• 제28권4호
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• pp.383-388
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• 2009

The dehydration responsive element binding protein 2C (DREB2C) is a dehydration responsive element/C-repeat (DRE/CRT)-motif binding transcription factor that induced by mild heat stress. Previous experiments established that overexpression of DREB2C cDNA driven by the cauliflower mosaic virus 35S promoter (35S:DREB2C) resulted in increased heat tolerance in Arabidopsis. We first analyzed the proteomic profiles in wild-type and 35S:DREB2C plants at a normal temperature ($22^{\circ}C$), but could not detect any differences between the proteomes of wild-type and 35S: DREB2C plants. The transcript level of DREB2C in 35S: DREB2C plants after treatment with mild heat stress was increased more than two times compared with expression in 35S:DREB2C plants under unstressed condition. A proteomic approach was used to decipher the molecular mechanisms underlying thermotolerance in 35S:DREB2C Arabidopsis plants. Eleven protein spots were identified as being differentially regulated in 35S:DREB2C plants. Moreover, in silico motif analysis showed that peptidyl-prolyl isomerase ROC4, glutathione transferase 8, pyridoxal biosynthesis protein PDX1, and elongation factor Tu contained one or more DRE/CRT motifs. To our knowledge, this study is the first to identify possible targets of DREB2C transcription factors at the protein level. The proteomic results were in agreement with transcriptional data.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 1996년도 학술발표회
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• pp.68-71
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• 1996

The thermal response of sprinkler is characterized by the response time index(RTI). The RTI represents the product of the thermal time constant for the heat responsive element of a sprinkler and the square root of the hot air velocity at plunge test. A plunge test is adapted for measuring RTI, wherein a sprinkler is suddenly immersed in the steady flow in the test section of a hot air tunnel. The method of measurements of the response parameters is presented.

• 한국화재소방학회논문지
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• 제9권1호
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• pp.20-29
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• 1995

The sprinkler head is a component of the sprinkler system intended to discharge water for automatic detection and extinguishment of fires. On this study, thermal characteristic values affecting the sensitivity of the fusible alloy type sprinkler head were obtained and analyzed under heated air stream condition which had constant temperature and velocity. The experiment was carried out under the forced convection condition with both the conductive heat loss considered and neglected. The thermal characteristic values of the sprinkler head were obtained in accordance with the material and shape of the heat responsive element and the conditions of the main body.

• BMB Reports
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• 제43권1호
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• pp.34-39
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• 2010

Expression patterns of OsAREB1 revealed that expression of OsAREB1 gene can be induced by ABA, PEG and heat. Yeast one-hybrid assay demonstrated it can bind to ABA-responsive element (ABRE), which was found in most stress-induced genes. Transgenic Arabidopsis over-expressing OsAREB1 had different responses to ABA and glucose compared to wild-type plants, which suggest OsAREB1 might have a crucial role in these two signaling pathways. Further analysis indicate that OsAREB1 have multiple functions in Arabidopsis. First, OsAREB1 transgenic plants had higher resistance to drought and heat, and OsAREB1 up-regulated the ABA/stress related gene such as RD29A and RD29B. Second, it delayed plant flowering time by down-regulating the expression of flowering-related genes, such as FT, SOC1, LFY and AP1. Due to the dates, OsAREB1 may function as a positive regulator in drought/heat stresses response, but a negative regulator in flowering time in Arabidopsis.

• Molecules and Cells
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• 제40권8호
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• pp.577-586
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• 2017

Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from Arabidopsis thaliana, which was designated AtCYS5. RT-qPCR analysis showed that the expression of AtCYS5 in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the ${\beta}-glucuronidase$ reporter gene under the control of the AtCYS5 promoter showed that AtCYS5 expression during seed germination was induced by HS and ABA. Constitutive overexpression of AtCYS5 driven by the cauliflower mosaic virus 35S promoter led to enhanced HS tolerance in transgenic Arabidopsis, which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout (cys5) plants grown under HS conditions. The HS tolerance of AtCYS5-overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and cys5. Although no HS elements were identified in the 5'-flanking region of AtCYS5, canonical ABA-responsive elements (ABREs) were detected. AtCYS5 was upregulated in ABAtreated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the AtCYS5 promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of AtCYS5 during seed germination and post-germination growth.

• 한국화재소방학회논문지
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• 제32권5호
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• pp.34-39
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• 2018

본 연구에서는 구획 공간 화재 시 발열량이 급격히 변하는 조건에서 스프링클러헤드의 손실인자 변화에 따른 작동시간을 분석하였다. 이를 위해서 내화보드로 구성된 구획 공간의 크기가 폭 0.3 m, 세로 0.5 m, 길이 3.0 m인 구조물을 제작하고 헵탄(n-Heptane) 풀 화재 실험을 수행하여 구획 공간 내부의 온도 분포, 헵탄의 질량감소율 그리고 발열량을 산출하였다. 또한, Fire Dynamics Simulator (FDS) Version 6.5를 사용하여 실험 조건과 동일한 발열량을 가정하고 스프링클러헤드의 설치위치 및 손실인자(C-factor) 변화에 따른 작동 시간을 분석하였다. 그 결과 반응시간지수(Response Time Index, RTI)가 $100(m{\cdot}s)^{0.5}$이고 작동온도가 $72^{\circ}C$인 스프링클러는 화원 상층부의 기류 온도가 $100^{\circ}C$에서 $300^{\circ}C$로 상승하고, 기류의 속도가 약 0.7 m/s인 경우 C-factor = 0과 1일 때 스프링클러의 작동시간은 최소 30 s~60 s, C-factor = 3일 때 62 s에서 최대 92 s, C-factor = 5일 때 120 s 이상으로 나타났다.

• Molecules and Cells
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• 제40권10호
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• pp.697-705
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• 2017

The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

• 한국화재소방학회논문지
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• 제27권3호
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• pp.8-13
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• 2013

건물 내부에서 화재 발생 시 외벽에 설치된 유리벽이나 창이 파손되며 이를 통해 화염이 밖으로 노출되면 화재는 외벽을 타고 수직상승하며 급격히 확대될 수 있다. 이를 막기 위한 효과적인 방법으로는 현재 내화유리를 사용하거나 또는 유리가 화염에 견딜 수 있도록 냉각수 등으로 방호해주는 윈도우 스프링클러 방식이 있다. 본 연구에서는 실내 유리창 상단에 설치되어 화재 시 열기류에 의해 감열체가 개방되면 유리에 냉각수를 방수하며 화염으로부터 유리를 방호해주는 윈도우 헤드를 개발하여 이의 내화성능을 평가하고자 하였으며 이를 위해 실규모 수직가열로를 이용해 유리창 시험체를 표준 온도-시간곡선에 따라 가열하여 윈도우 헤드의 내화시험을 실시하였다. 실험결과 1개의 윈도우 헤드로 최대 너비 2,400 mm의 유리창을 2시간 동안 방호하는데 성공하였으며 이 때 유리창 이면의 최고온도는 국소적으로 $126^{\circ}C$까지 상승하였으나 평균 $100^{\circ}C$를 유지하며 안정된 내화성능을 나타내었다.