• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.1054-1057
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• 2007

Lipases are industrially useful versatile enzymes that catalyze numerous different reactions including hydrolysis of triglycerides, transesterification, and chiral synthesis of esters under natural conditions. Although lipases from various sources have been widely used in industrial applications, such as in food, chemical, pharmaceutical, and detergent industries, there are still substantial current interests in developing new microbial lipases, specifically those functioning in abnormal conditions. We screened 17 lipase-producing yeast strains, which were prescreened for substrate specificity of lipase from more than 500 yeast strains from the Agricultural Research Service Culture Collection (Peoria, IL, U.S.A.), and selected Yarrowia lipolytica NRRL Y-2178 as a best lipase producer. This report presents new finding and optimal production of a novel extracellular alkaline lipase from Y. lipolytica NRRL Y-2178. Optimal culture conditions for lipase production by Y. lipolytica NRRL Y-2178 were 72 h incubation time, $27.5^{\circ}C$, pH 9.0. Glycerol and glucose were efficiently used as the most efficient carbon sources, and a combination of yeast extract and peptone was a good nitrogen source for lipase production by Y. lipolytica NRRL Y-2178. These results suggested that Y. lipolytica NRRL Y-2178 shows good industrial potential as a new alkaline lipase producer.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제24권3호
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• pp.141-150
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• 2013

Objectives : The objective of this report is to identify the utilization of hospital school service during hospitalization among patients in their childhood and adolescence with psychiatric disorders. Methods : We retrospectively reviewed the medical record of child and adolescent psychiatric who were hospitalized during March 2009 through October 2012. We compared the one-year successful schooling and outpatient follow up rate between users and nonusers of the inpatient hospital school service. The hospital schooling experiences of the users were investigated upon follow-up visits to the outpatient clinic. Results : Sixty-three students received hospital school service during hospitalization among total 122 child and adolescent inpatients. Hospital school participants showed a significantly higher school reentry rate (61.9%) than non-participants (40.7%). However, there was no difference on follow up rate between the two groups. More than 60% of the 22 interviewed participants expressed an above-average level of satisfaction about hospital school service. Conclusion : Many patients with mental illness experience difficulty in receiving school education during treatment. That induces deterioration in disease, academic failure, poor social skills, low self-esteem, economic difficulties, and future job opportunities. The results of this study emphasize the importance of hospital school service and offer useful guidance for hospital school operation.

• 한국산업보건학회지
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• 제15권2호
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• pp.135-143
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• 2005

This study was conducted to measure the exposure levels to noise for the middle and the high school students who worn headsets in the PC rooms. The noise levels from the headsets were measured with noise dosimeters, and the statistical relationships between the utilization pattern, user's views on the PC rooms and it's environment were analyzed for the selected PC rooms in the Ahnshan area. The results were as follows; 1. No significant difference was found in the number of uses and average time spent per day by education level and by sex of the users(p>0.05). 2. No statistical significant difference was found between the contents selected by education level of the users (p>0.05). The male participants overwhelmingly enjoyed the PC games (98.3 %) while females selected for listening music (75.0 %) with significant difference(P<0.05), followed by browsing internet and PC communications (70.0 %), PC game (66.7 %) and chatting (33.3 %). 3. The actual noise measurements of the headsets worn by the users of different sexes and educational backgrounds produced no differences(p>0.05), while a significant difference was found between genders (p<0.05). 4. For sound pressure level measurements, the weighted average(Lavg) of 84.5 dB(A) on the left headsets exceeded that of 79.8 dB(A) on the right headsets significantly(p<0.01) and the maximum value of 96.9 dB(A) on the left headsets exceeded the maximum value of 93.5 dB(A) on the right headsets(p<0.01). 5. The actual noise measurements of the headsets worn by the users of different sexes and educational backgrounds produced no differences(p>0.05), however, the contents selected by the users with different educational backgrounds were noted with significant difference(p<0.05) while no difference was found by gender(p>0.05).

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1555-1562
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• 2010

Development of the strain and the fermentation process of Hansenula polymorpha was implemented for the production of ${\gamma}$-linolenic acid ($GLA,\;C18:3{\Delta}^{6,9,12}$), an n-6 polyunsaturated fatty acid (PUFA) that has been reported to possess a number of health benefits. The mutated ${\Delta}^6$-desaturase (S213A) gene of Mucor rouxii was expressed in H. polymorpha under the control of the methanol oxidase (MOX) promoter. Without the utilization of methanol, a high-cell-density culture of the yeast recombinant carrying the ${\Delta}^6$-desaturase gene was then achieved by fed-batch fermentation under glycerol-limited conditions. As a result, high levels of the ${\Delta}^6$-desaturated products, octadecadienoic acid ($C18:2{\Delta}^{6,9}$), GLA, and stearidonic acid ($C18:4{\Delta}^{6,9,12,15}$), were accumulated under the derepression conditions. The GLA production was also optimized by adjusting the specific growth rate. The results show that the specific growth rate affected both the lipid content and the fatty acid composition of the GLA-producing recombinant. Among the various specific growth rates tested, the highest GLA concentration of 697 mg/l was obtained in the culture with a specific growth rate of 0.08 /h. Interestingly, the fatty acid profile of the yeast recombinant bearing the Mucor ${\Delta}^6$-desaturase gene was similar to that of blackcurrant oil, with both containing similar proportions of n-3 and n-6 essential fatty acids.

• 대한예방한의학회지
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• 제22권2호
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• pp.25-32
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• 2018

Objectives : This study was conducted to investigate the frequent diseases among the people who had been treated by collaborative treatment of Korean medicine and Western medicine, and to compare their medical use behaviors before and after the collaborative first-stage pilot project between Korean medicine and Western medicine. Methods : 4,467 patients were identified as the patients who are participated in the collaborative first-stage pilot project between Korean medicine and Western medicine during the period between July 15, 2016 and March 31 2017. We used 28,480 records of Korean national health insurance claim data from January 1 2016 to March 31 2017 to analyzed present condition of cooperative medical usage. Also we conducted a paired t test to compare the percentage of collaborative treatment days before and after the pilot project period. Results : We found that the most frequent diseases treated in the pilot project were the diseases of musculoskeletal and nervous system and then 10 major diseases such as H, K, J, C (D), N, L, E, A, H and F disease codes in order. Also it was confirmed that 14 major and 53 medium diseases are included from more than 90% of total patients. As a result of high frequency of medical treatment in Western medicine or Korean medicine is "administration of Korean medicine", which is same as before and within the pilot project. The ratio of utilizing both Western and Korean medical care for the same disease on the same day by both general patients and patients in KCD-7 disease code group C, G, I, M and S had been increased significantly. Conclusions : The pilot project might change the behavior of utilizing the medical care service by increasing the ratio of collaborative treatment of Western medicine and Korean medicine for the same disease on the same day.

• 한방재활의학과학회지
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• 제26권3호
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• pp.85-95
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• 2016

Objectives Military medicine is the study of research which includes health, sanitation, clinic, and quarantine in military personnel. This study is focused on research the acupuncture treatment of military medicine. Methods The study on acupuncture research for Military Medicine was referred from via KoreaMed, OASIS, The Journal of Korean Military Medical Association, KMbase, KAMJE, NDSL, KISS, KISTI, PubMed, MEDLINE, EMBASE, and The Cochran library. As a result, 18 research papers were found and they were analyzed according to the year of publishment, the titles of journals, the nation of research center, the types of study, the purpose of study, the outcome of measurement, the type of acupuncture, and the assessment of acupuncture effect. And then assessed the risk of bias about RCT, NRCT, prospective cohort studies, and before and after studies. Results The number of the published research papers on acupuncture was increased since 2013. The studies were mainly published in the USA and eight out of 18 were RCT. The purpose of study is mostly for musculoskeletal and psychological disease and medical decision for acupuncture. The type of the acupuncture used is not only traditional acupuncture but also the auricular acupuncture. Even though the research results about the effect of acupuncture were excellent and positive, some of studies had a high risk of bias. Conclusions The studies state about acupuncture treatment as mostly positive and effective, however, further clinical studies are required to obtain objective clinical evidence of acupuncture treatment. Furthermore, future trials would be needed to find out effective acupuncture treatments on military duty.

• 디지털콘텐츠학회 논문지
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• 제18권2호
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• pp.267-274
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• 2017

최근 패션, 뷰티 업계를 중심으로 스마트 미러를 도입한 매장을 선보이는 등 IoT기술을 적용하고자 다양한 노력이 시도되고 있다. 스마트 미러는 거울을 통해 모습을 비춰볼 수 있으면서 거울 뒤에 부착된 화면을 통해 원하는 내용을 디스플레이 할 수 있어 패션, 뷰티, 헬스 케어 등 다양한 산업 분야에 적용이 가능하다. 본 논문에서는 이러한 스마트 미러의 특성을 이용하여 구술 능력이 퇴화되어 발음이 부정확한 청각 장애인이 타인의 도움 없이 스스로 발음 교정 학습을 할 수 있도록 하는 학습 시스템을 제안하고자 한다. 제안된 시스템에서는 임베디드 시스템에서 적용 가능한 효과적인 립 리딩 기법을 제안하여 기존에 검증된 발음 교정용 데이터와 연동하여 학습 효율을 높였다.

• 한국통신학회논문지
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• 제42권3호
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• pp.686-697
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• 2017

고령자들의 정신적/육체적 건강 유지를 위해 디지털 메디신 (Digitial Medicine)의 일환으로 일상에서의 운동 프로그램을 사용하는 시도가 다양하게 이루어지고 있다. 특히 웨이러블 기기에 부착된 센서를 활용한 운동 처방과 분석 방법은 IoT 기술을 헬스케어 분야에 적용시키는 대표적인 예로 제시된다. 본 연구에서는 현재 수원시 노인정신건강센터에서 운영하는 운동 프로그램에서 기존에 수기로 수집되고 작성되는 데이터 분석의 신뢰성을 개선하고 노인들의 운동 동기를 촉구하고자 스마트밴드와 BLE 스캐너 장치를 활용하여 IoT(Internet of Thing)기반 행동 인터벤션(intervention) 시스템을 구현한다. 실제 운동 처방과 운동 데이터 분석을 통해 행동 인터벤션 시스템을 테스트하여 데이터 수집과 분석의 무결성을 검증하고 시스템의 개발과 적용에 대한 일련의 과정을 제시한다.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.681-698
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• 2022

The Nile tilapia Oreochromis niloticus, Atlantic salmon Salmo salar, rainbow trout Oncorhynchus mykiss, olive flounder Paralichthys olivaceus, common carp Cyprinus carpio, grass carp Ctenopharyngodon idella and rohu carp Labeo rohita are farmed commercially worldwide. Production of these important finfishes is rapidly expanding, and intensive culture practices can lead to stress in fish, often reducing resistance to infectious diseases. Antibiotics and other drugs are routinely used for the treatment of diseases and sometimes applied preventatively to combat microbial pathogens. This strategy is responsible for the emergence and spread of antimicrobial resistance, mass killing of environmental/beneficial bacteria, and residual effects in humans. As an alternative, the administration of probiotics has gained acceptance for disease control in aquaculture. Probiotics have been found to improve growth, feed utilization, immunological status, disease resistance, and to promote transcriptomic profiles and internal microbial balance of host organisms. The present review discusses the effects of single and multi-strain probiotics on growth, immunity, heamato-biochemical parameters, and disease resistance of the above-mentioned finfishes. The application and outcome of probiotics in the field or open pond system, gaps in existing knowledge, and issues worthy of further research are also highlighted.

• 대한본초학회지
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• 제32권5호
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• pp.19-25
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• 2017

Objective : The Sinseon-yukza-hwan (SSY), a Korean medicinal formula which includes Radix Rehmanniae Preparata and other medicinal herbs, has long been used for treatment of alopecia and gray hair through oral administration. This study is designed to enhance the utilization of natural materials in hair and scalp-related cosmetics. Possibility of SSY as an antioxidant was examined from its 50% ethanol extract. Methods : The antioxidative capacities were evaluated by determining total phenolic and flavonoid contents, 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power and hydroxyl radical scavenging activity. Results : Total polyphenol and flavonoid contents of SSY were 25.53 mg TE, tannic acid equivalent/g and 18.90 mg RE, rutin equivalent/g, respectively, which correlated strongly its antioxidative activity. The DPPH and ABTS free radical scavenging activities of SSY at $0.1mg/m{\ell}{\sim}5mg/ml$ were ranged from 20% to 85% and 10% to 58%, respectively. Also the hydroxyl radical scavenger activity and reducing power increased in SSY-treated group, which were significantly lower in SSY-compared to BHA-treated group. But the highest reducing power was shown as 79% from SSY-treated group, which was higher value than 65% from BHA-treatment. These results showed that SSY extract effectively inhibited the generation of free radicals in the all assay system with dose-dependent manners. Conclusions : Thus, the present study provide preclinical data to support the expanded application of SSY, which could be potential candidates for natural antioxidants.