• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.696-703
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• 2020

Objective: Cattle were some of the first animals domesticated by humans for the production of milk, meat, etc. Long noncoding RNA (lncRNA) is defined as longer than 200 bp in nonprotein coding transcripts. lncRNA is known to function in regulating gene expression and is currently being studied in a variety of livestock including cattle. The purpose of this study is to analyze the characteristics of lncRNA according to sex in Hanwoo cattle. Methods: This study was conducted using the skeletal muscles of 9 Hanwoo cattle include bulls, steers and cows. RNA was extracted from skeletal muscle of Hanwoo. Sequencing was conducted using Illumina HiSeq2000 and mapped to the Bovine Taurus genome. The expression levels of lncRNAs were measured by DEGseq and quantitative trait loci (QTL) data base was used to identify QTLs associated with lncRNA. The python script was used to match the nearby genes Results: In this study, the expression patterns of transcripts of bulls, steers and cows were identified. And we identified significantly differentially expressed lncRNAs in bulls, steers and cows. In addition, characteristics of lncRNA which express differentially in muscles according to the sex of Hanwoo were identified. As a result, we found differentially expressed lncRNAs according to sex were related to shear force and body weight. Conclusion: This study was classified and characterized lncRNA which differentially expressed by sex in Hanwoo cattle. We believe that the characterization of lncRNA by sex of Hanwoo will be helpful for future studies of the physiological mechanisms of Hanwoo cattle.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.66-73
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• 2018

Hanwoo is an important livestock resource in Korea. Its genetic improvements of economic traits have mainly focused on the steers in the past. However, there is a great necessity to extend the breed improvement programs to the cows as well. Therefore, the objective of this study was to investigate the effects of various environmental factors (person taking the measurement, region, year of measurement, month of measurement, image interpreter, birth-year and birth-year) on ultrasound measured carcass traits. A total of 27,215 ultrasound measurements of carcass traits were recorded between 2004 and 2012 for 22,620 cows born from 1997 to 2011. The ultrasound measures included backfat thickness (BFT), eye muscle area (EMA), and marbling score (MAR). The mean values for the BFT, EMA and MAR were 4.46 mm, $56.24cm^2$, and 4.12 point, respectively. Seven environmental factors, person taking the measurement, region, year of measurement, month of measurement, image interpreter, birth-year and birth-month, were tested to determine if they had a significant effect on the studied traits using the GLM procedure in SAS. All factors were found to significantly affect all the ultrasound carcass traits in this study. Unlike in previous studies, among the environmental effects, the significant effect of the image interpreter on the ultrasound carcass traits was shown for the first time in this study. These results indicate that future genetic evaluations of ultrasound carcass traits of Hanwoo cows should include all of the above environmental factors as well as the effect from people taking the measurements.

• Journal of Embryo Transfer
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• v.14 no.2
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• pp.83-88
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• 1999

This study was undertaken with three objectives : to determine the optimal time of blood collection for plasma urea nitrogen(PUN) analysis, to examine the frequency distribution of PUN levels in recipient herd and to relate concentration of PUN to conception rate in Hanwoo recipients. The relationship between PUN level and time postfeeding was examined for 5 individual cows. Mean concentration of PUN rose for 4hrs postfeeding and decreased to PUN level before feeding at 10hrs postfeeding. And then, the blood for PUN analysis was collected at the time before feeding in next experiments. The ratio of cows with PUN concentration of < 12, 12∼18 and 18mg/dl were 50.6, 39.9 and 9.5% in 163 recipients, individually. The pregnancy rate of cows with PUN concentration 12∼16 mg/dl (63.3%) was higher than that of cows with PUN concnetration < 12 mg/dl (46.7%) or > 16mg/dl (42.9%). There results suggest that the PUN test may be beneficial for management of recipient herd in effects to maintain or improve reproductive efficiency.

• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.255-262
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• 2009

Current study was conducted to investigate the influence of parity and region variation in ultrasound live carcass traits of Hanwoo cows. A total of 1,386 heads of Hanwoo cows, spreading over six regions in Gangwon province of South Korea, were measured for longissimus muscle area, back fat thickness, marbling score and rump fat thickness by using real-time ultrasound method. The results revealed that, positive correlations were found in all ultrasound live carcass traits. All live carcass traits were increased in accordance with parity until the sixth, and then decreased thereafter. Statistical significant differences (p<0.01) were found among regions in ultrasound live carcass traits, e.g. Hanwoo cows showed highest longissimus muscle area and marbling score in Hwacheon, while the lowest back fat thickness and rump fat thickness were observed in Yanggu. Greater longissimus muscle area and marbling scores were found in Hanwoo cows which were born during autumn, 2000. Moreover, individuals born during autumn, 2005 and 2006 showed a thicker back fat and rump fat thickness. Back fat thickness had the highest positive correlation with rump fat thickness, while rib eye area and rump fat thickness had the lowest correlation.

• Journal of Animal Science and Technology
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• v.62 no.4
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• pp.449-459
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• 2020

Here, we investigated the effects of different nutrient requirements (NR) on blood metabolites, transferable embryo number after multiple superovulations with artificial insemination (AI), body condition score (BCS), and estrus cycle in Hanwoo cow. Nineteen Hanwoo cows were randomly divided into three groups (80%, 100%, and 120% NR, containing 6, 8, and 5 individuals, respectively) and fed based on the NR. In experiment 1, glucose, total cholesterol, triglyceride, blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), non-esterified fatty acids (NEFA), albumin (ALB), and total protein (TP) were analyzed. In experiment 2, total number of recovered embryos and transferable embryos was examined after embryo recovery and multiple superovulations with AI. In experiment 3, body weight, BCS, and estrus cycle were examined. In experiment 1, total cholesterol was significantly different among the 80%, 100%, and 120% NR groups (126.5 ± 3.3, 152.6 ± 2.4, and 177.4 ± 1.8 mg/dL, respectively, p < 0.05). The triglyceride and BUN levels in the 120% NR group were significantly higher than those in the 80% and 100% groups (p < 0.05). The NEFA levels were significantly different among the 80%, 100%, and 120% NR groups (440.5 ± 18.2, 318.5 ± 23.1, and 195.1 ± 8.5 ЧEq/L, respectively, p < 0.05). The AST and TP levels in the 80% NR group were significantly lower than those in the 100% and 120% NR groups (p < 0.05). In experiment 2, the 120% NR group showed a higher percentage of transferable embryos than the 80% and 100% groups (p < 0.01). The mean body weight and BCS among the 80%, 100%, and 120% NR groups were significantly different (p < 0.05). The estrus cycle in the 80% NR group was delayed compared with the 100% and 120% NR groups (20.8 ± 0.2 and 21.2 ± 0.5 days, respectively). In conclusion, the blood metabolic tests proved that Hanwoo cows with 120% NR can produce a large number of transferable embryos. Thus, 120% NR is the appropriate feeding level for this type of cows as it results in the production of a large number of transferable embryos by multiple superovulations with AI.

• Journal of Embryo Transfer
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• v.26 no.1
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• pp.9-12
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• 2011

This study was undertaken to determine whether the presence of fertility-associated antigen (FAA) in semen would influence semen characteristics and conception rate of artificial insemination in Hanwoo. The response to FAA of 36 heads of proven bull, 7 heads of young bull, and 27 heads of performance-tested bull was that one proven bull was FAA-negative and the others were FAA-positive, therefore FAA-negative bull was 1.4%. FAA-negative bull was lower in first and second semen concentrations than those of FAA-positive bull in 5,301 semen of 21 heads of proven bull, then FAA-negative bull was fewer as 11.5% in total sperm counts. The estrus of 22 heads was 70d-nonreturned in 36 cows first inseminated with frozen semen of FAA-negative bull, but that of 249 heads in 378 cows first inseminated with frozen semen of FAA-positive bull. Each conception rate was 61.1% and 65.9%, respectively. The difference of conception rates was 4.8%. These results indicate that the response of FAA to semen were influenced semen characteristics and conception rate of artificial insemination, but further investigations are needed to confirm the results.

• Journal of Embryo Transfer
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• v.29 no.4
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• pp.321-325
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• 2014

The present study was performed on farm animals to test the effectiveness of progesterone-releasing intravaginal device (Cue-Mate$^{(R)}$ 1.56 g) and injection of prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) for synchronization estrus in Hanwoo cattle. The cattle were at random stage of the estrus cycle. The cows were artificially inseminated at day 7 after Cue-Mate withdrawal, using commercial semen from Korean native bulls. There was a season effect on the estrus synchronization rate. It was higher in spring (94.3%) followed by winter (93.3%), autumn (90.4%) and summer (67.2%). In summary, The results of this study revealed that season has influences on estrus behavior of cattle with no significant effect on pregnancy rate. In summary, we suggest summer reproductive management to alleviate the effects of heat stress. It should be based on intensive cooling combined with hormonal treatment. Given that different subgroups of cows benefit differently from the treatments, selective hormonal administration should be considered.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.23-23
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• 2017

Recent favor on high quality food and concern on food safety have demonstrated the superiority of Hanwoo (Korean native cattle). In general, the price of cow is higher than those of steer and bull, causing cheating issues in the market. Hence, this study is to discriminate genders of Hanwoo with identification of factors which highly influence gender discrimination based on the big-size deboning data. Totally, there were 31 variables in the deboning data, and we divided into them two categories: data obtained before and after deboning. Discriminant function analysis was then applied into the data to determined the accuracy of gender discrimination in Hanwoo. The result showed that Hanwoo could be classified by gender with 99.2% of accuracy when using all 31 variables. In detail, it was possible to identify 93 of 94 bulls (98.9%), 96 of 96 cows (100%) and 74 of 75 steers (98.7%). The most significant variables was chuck, sirloin, armbone shin, plates, retail and cuts percentage, sequentially. With variables obtainable before deboning, accuracies of classification were 91.5% for bulls, 92.7% for cows, and 89.3% for steers. The most significant variables was water, cold carcass weight and back-fat thickness. The discrimination accuracy was higher with data obtainable after deboning: bulls (98.9%), cows (99.0%) and steers (98.7%). In this case, chuck, sirloin and armbone shin were the factors determined the classification ability. This study showed that Hanwoo can be classified based on deboning data with appropriate statistics, further suggesting weight of cut of beef might be the standard for gender classification.

• Journal of Embryo Transfer
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• v.17 no.2
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• pp.153-162
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• 2002

In order to develop the breeding techniques to increase Hanwoo (Bos taurus coreanae) population, the present study was performed 1) to establish the treatment protocol on reproductive disorders with GnRH or PGF／sub 2$\alpha$／, 2) to improve intraovarian PG $F_{2}$$\alpha$／ administration for reducing open period. Among total of 43 diagnosed, high percentage of cows (41.9％, 18 cows) were diagnosed as silent heat, followed by inactive ovaries (32.6％, 14 cows), ovarian cysts (9.3％, 4 cows), persistent corpus luteum (7.0％, 3 cows), endometriosis (4.7％, 2 cows), pyometra (2.3％, 1 cow) and luteal cysts (2.3％, 1 cow). To treat silent heat, 18 cows were administrated with 25 mg PGF／sub 2$\alpha$／, heat-detected, artificially inseminated and monitored pregnancy. All treated cows were heat-detected and 16 cows (88％) were successfully pregnant. With 200 $\mu\textrm{g}$ GnRH treatment, 7 cows (70％) with inactive ovaries and 3 cows (75％) with ovarian cysts were successfully pregnant. Administration with 25mg PGF／sub 2$\alpha$／, successfully treated 3 cows (100％) with persistent corpus luteum and 1 cow (100％) with luteal cysts, followed by 100％ pregnancy rate. With the combined treatment of 25 mg PG $F_{2}$$\alpha$／and antibiotics, 2 cows (100％) with endometriosis were effectively treated and got pregnant after. artificial insemination (AI). In order to reduce open period, 5 mg PGF／sub 2$\alpha$／ was administrated intraovarian to 20 days cows after delivery, heat-detected, artificially inseminated and monitored pregnancy. In the first experiment, in order to recover uterus, 5mg PGF／sub 2$\alpha$／were administrated, followed by administration of 5mg PGF／sub 2$\alpha$／ at the interval of 14 days. As results, 74％ (17／23 cows) of pregnancy rate after AI. In order to further reduce the open period, 5 mg PGF／sub 2$\alpha$／was administrated at the interval of 11 days without the period of uterus recovery, resulted in 94％ (16／17 cows) pregnancy rate. In conclusion, these results showed that PGF／sub 2$\alpha$／ and GnRH treatment were effective hormonal treatment resume in Hanwoo with various reproductive disorders. In addition, modified protocol of intraovarian PGF／sub 2$\alpha$／ administration could be the effective method for reducing the open period.

• Journal of agriculture & life science
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• v.50 no.4
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• pp.113-125
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• 2016

This study was conducted to investigate the effect of low feeding level of concentrate after sexual maturity(14 month old) on the growing and breeding performances in Hanwoo cows. Eighteen cows(average body wt. 251kg) were alloted to two groups by completely randomized design, and fed a commercial formula feed by 0.8%(T1) and 1.2%(T2) of body weight for 30 months of total experimental period(feeding trial of 20 months, breeding trial of 10 months). When the results of this experiment compared to the Korean feeding standard for Hanwoo(2012), the reason of low growing performance in T1 was induced from low protein intake rather than energy. The change of body size by feeding levels was significantly appeared at chest width and body length, particularly chest width of cows in T1 was rather reduced by 3.7% in the final than the initial measurement. Ovarian size of T1 was only 63% of T2, and pregnancy rate(33.3% : 77.8%) and birth wight of calf(22.5kg : 29.5kg) were also seriously repressed by low intake of dietary protein after sexual maturity in Hanwoo cow. It was appeared that the growing and breeding performances of Hanwoo cows were more affected by the level of dietary protein rather than energy in the feeding after sexual maturity.