Abstract
In order to develop the breeding techniques to increase Hanwoo (Bos taurus coreanae) population, the present study was performed 1) to establish the treatment protocol on reproductive disorders with GnRH or PGF/sub 2$\alpha$/, 2) to improve intraovarian PG $F_{2}$$\alpha$/ administration for reducing open period. Among total of 43 diagnosed, high percentage of cows (41.9%, 18 cows) were diagnosed as silent heat, followed by inactive ovaries (32.6%, 14 cows), ovarian cysts (9.3%, 4 cows), persistent corpus luteum (7.0%, 3 cows), endometriosis (4.7%, 2 cows), pyometra (2.3%, 1 cow) and luteal cysts (2.3%, 1 cow). To treat silent heat, 18 cows were administrated with 25 mg PGF/sub 2$\alpha$/, heat-detected, artificially inseminated and monitored pregnancy. All treated cows were heat-detected and 16 cows (88%) were successfully pregnant. With 200 $\mu\textrm{g}$ GnRH treatment, 7 cows (70%) with inactive ovaries and 3 cows (75%) with ovarian cysts were successfully pregnant. Administration with 25mg PGF/sub 2$\alpha$/, successfully treated 3 cows (100%) with persistent corpus luteum and 1 cow (100%) with luteal cysts, followed by 100% pregnancy rate. With the combined treatment of 25 mg PG $F_{2}$$\alpha$/and antibiotics, 2 cows (100%) with endometriosis were effectively treated and got pregnant after. artificial insemination (AI). In order to reduce open period, 5 mg PGF/sub 2$\alpha$/ was administrated intraovarian to 20 days cows after delivery, heat-detected, artificially inseminated and monitored pregnancy. In the first experiment, in order to recover uterus, 5mg PGF/sub 2$\alpha$/were administrated, followed by administration of 5mg PGF/sub 2$\alpha$/ at the interval of 14 days. As results, 74% (17/23 cows) of pregnancy rate after AI. In order to further reduce the open period, 5 mg PGF/sub 2$\alpha$/was administrated at the interval of 11 days without the period of uterus recovery, resulted in 94% (16/17 cows) pregnancy rate. In conclusion, these results showed that PGF/sub 2$\alpha$/ and GnRH treatment were effective hormonal treatment resume in Hanwoo with various reproductive disorders. In addition, modified protocol of intraovarian PGF/sub 2$\alpha$/ administration could be the effective method for reducing the open period.
본 연구에서는 한우의 신속한 증식을 위한 번식기술 개발을 위해 발정이 재귀되지않은 무발정 한우의 번식장애 치료효과와 공태기를 감축하기 위한 난소실질내 PGF/sub 2$\alpha$처치효과를 알아보았다. 1. 번식장애의 감별진단 결과는 검사두수 43두중 둔성발정 또는 발정발견의 잘못이 18두 (41.9%)로 가장 많았고, 난소기능정지 14두 (32.6%), 난포낭종 4두(9.3%), 영구황체 3두 (7.0%), 자궁내막염 2두 (4.7%), 자궁축농증과 황체낭종은 각각 1두(2.3%)로 나타났다. 2. 둔성발정 또는 발정발견의 잘못 18두를 PGF/sub 2$\alpha$/ 25 mg으로치료하여 18두가 발정이 발현되었고 이중 16두 임신되었다. 3. 난소기능정지의 경우는 GnRH 200$\mu\textrm{g}$으로 치료하여 10두중 8두가 발정이 발현되어 이중 7두가 임신이 되었으며, 난포낭종도 역시 CnRH 200$\mu\textrm{g}$으로 치료하여 4두중 3두가 발정이 발현되어서 인공수정후 임신이 되었다. 4. 영구황체 3두와 황체낭종 1두는 각각 PGF/sub 2$\alpha$/ 25 mg으로 치료하여 3두 모두 발정이 발현되어 수정후 임신이 되었다. 5. 자궁내막염 2두는 PGF/sub 2$\alpha$/25mg과 항생제 요법을 병행한 결과 2두 모두 발정이 발현되어서 인공수정후 임신이 되었다. 6.공태기를 줄이기 위한 방법으로 분만 후 20일이 경과한 소에 대해 14일 간격으로 PGF/sub 2$\alpha$/의 난소실질내 투여시 공여동물 23두중 17두에서 임신이 확인되어 74%의 수태율을 보였다. 또한 11일 간격으로 PGF/sub 2$\alpha$/ 를 투여한 군에서는 17두중 16두가 임신되어 94%의 높은 수태율을 보여 공태기를 더 단축할 수 있는 우수한 처리법임을 알 수 있었다.