• Korean Journal of Organic Agriculture
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• v.31 no.4
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• pp.469-477
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• 2023

Animal welfare has been gradually gathering more attention from consumers over time, making it increasingly important to assess the level of stress experienced by livestock. Traditionally, stress has been measured by collecting blood to assess cortisol levels, an action that can be considered distressing for the animal. Therefore, we aimed to explore the feasibility of using hair as an alternative medium to blood for stress assessment. We utilized B/F (blood collected at the farm), B/A (blood collected after transport to the auction), and H/A (hair collected at the auction after blood sampling) from calves at the age of 7-9 months transported from the farm to the auction. Hair underwent a washing and extraction process to utilize hair extracts, while blood was centrifuged to analyze using ELISA. The cortisol concentration in the blood was significantly higher in B/A compared to B/F (p<0.05), confirming that the calves experienced stress during transportation. Additionally, H/A was significantly lower than both B/A and B/F (p<0.0001). These results emphasized that cortisol in hair is not suitable for investigating short-term stress in livestock, as it is with blood. While measuring stress indices using hair may not be appropriate for replacing blood, it is considered a highly suitable practice for animal welfare, and further research in this area should be continued.

• YAKHAK HOEJI
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• v.48 no.3
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• pp.207-212
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• 2004

An analytic method was developed for the quantitation of $\Delta$$^{9}-$ tetrahydrocannabinol (THC) and 11-nor-9-carboxy THC (THC-COOH) in human hair. After hair samples were pulverized using Freezer Mill, deuterated internal standards were added and digested in 1 N NaOH at $100^{\circ}C$ water bath for 30 min. Digest solutions were extracted by 5 ml hexane：ethyl acetate (90：10) after acidification with acetic acid. The organic phase was evaporated under N 2 and derivatized by BSTFA (with 1% TMCS) at $85^{\circ}C$ for 45 min. The derivatized solution was separated on HP-5MS column ($30m{\times}0.25mm{\times}0.25mm$) and detected using EI-GC-MS with selective ion monitoring mode. The assay of calibration was ranged from 5 to 100 ng/50 mg hair ($r^2$＞0.99) for THC and THC-COOH. Within and between-run precision were calculated at 6, 30, 60 ng/50 mg hair with coefficients of variation less than 11%. Within and between run accuracies at the same concentrations were$\pm$14% and $\pm$30% of target for both analytes, respectively. Absolute and relative recovery at 10 and 100 ng were 60∼91％. The method was used to detect and quantify THC and THC-COOH in cannabis abuser's hairs (N = 16) and SRM (N=5, THC 1 ng/mg, NIST). We detected THC and THC-COOH in only one hair sample. In SRM, % accuracy was 93% (range 86∼103%) and precision (% CV) was 8.14. We began to set up a quantitative analysis of THC and THC-COOH using EI-GC-MS. Continuously, we need to modify and develop this method in order to apply for identification in cannanbis users' hair.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.169.2-169.2
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• 2003

A method using EI-GC-MS is described for the determination of amphetamines in hair. The method is applied to simultaneous quantify amphetamines (methamphetamine, amphetamine, MDMA and MDA). Drugs were extracted in 1 ％ HCl in methanol from hair. After derivertization with TFAA, the resulting drugs were separated on HP-5MS column during a 16 min program and identified by mass spectrometry with the SIM mode(EI-GC-MS). (omitted)

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.5 s.142
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• pp.715-726
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• 2005

The purpose of this study was to identify the effects of the sensation seeking and body image on female adolescents' clothing behavior and hair style attitude. The data was collected from 390 high school girls living in Seoul, via a self·administered questionnaires, and were analyzed by frequency, factor analysis, reliability test, Pearson's correlation, and multiple regression. The results of this study was as follows : 1) Three factors of body images were self appearance evaluation, appearance orientation and fitness orientation. Five factors of clothing attitudes were fashion/clothing interest, clothing exhibition, social approval, psychological dependence on clothing and fashion leadership. And three factors of hair style attitudes were interest in hair style, individuality in hair style and dependency on hair designer. 2) As the result of analyzing the effects of sensation seeking and body image on clothing behavior, fashion/clothing interest was explained by appearance orientation and fitness orientation, and clothing exhibition by sensation seeking and appearance orientation, and social approval by appearance orientation, self appearance evaluation and body cathexis, and fashion leadership by appearance orientation, fitness orientation and self appearance evaluation, and clothing conformity to the self-entertainer by appearance orientation, sensation seeking and fitness orientation, and appearance related delinquency by sensation seeking, appearance orientation, fitness orientation, self appearance evaluation. 3) As the result of analyzing the effects of sensation seeking and body image on hair style attitudes, interest in hair style was explained by the factors such as appearance orientation, sensation seeking, fitness orientation, and body cathexis, and individuality in hair style by appearance orientation, sensation seeking and self appearance evaluation, and dependency on hair designer by body cathexis.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.19 no.4
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• pp.259-268
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• 2016

Purpose: Zinc deficiency can induce serious clinical problems in the gastrointestinal (GI) system and immune system and can affect growth and development. It is more severe in younger patients. Chronic zinc deficiency is reflected more precisely in hair than in serum. We studied hair zinc levels and other hair and serum micronutrients in chronic malnourished children to identify which micronutrients are affected or correlated with the other ones. Methods: Hair mineral analyses were performed in 56 children (age, 1-15 years) presenting with malnutrition, poor growth, poor appetite, anorexia, with/without other GI symptoms (diarrhea, abdominal pain, constipation) from August 2012 to March 2015. Biochemical studies for macronutrients and major micronutrients were also conducted. Results: Hair zinc deficiency was diagnosed in 88%, and serum zinc deficiency was diagnosed in 55% of the children. There was no statistical correlation between serum and tissue zinc level. Hair zinc levels were highly correlated with serum vitamin D (r=-0.479, p=0.001), which also showed correlation with hair levels of magnesium and calcium. (r=0.564, 0.339, p=0.001, 0.011). Hair calcium level was correlated with serum pre-albumin (r=0.423, p=0.001). These correlations may explain the phenomenon that the major clinical manifestation of zinc deficiency is poor body growth. Clinical symptoms were resolved in most children after zinc supplementation. Conclusion: Hair zinc and mineral analyses are useful as a therapeutic guide in the clinical investigation of children with malnutrition and poor growth.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.1
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• pp.65-80
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• 2016

Objective : The purpose of this study is to report the hair regrowth effect of Gagamyeonryunggobon-dan on ICR mice from measuring the change of diverse factors.Methods : Gagamyeonryunggobon-dan was treated by oral administration with 2.5㎎/㎏/day amount for 3 weeks per mouse everyday. Hair regrowth was estimated by change of morphology, angiogenesis, hair follicle activation. The change of morphology was observed with external, internal change and sebaceous gland. Angiogenesis was estimated by image analysis, capillary distribution and angiogenic chemokine(MIP-2). Hair follicle activation was estimated by PCNA, IGF-2 and serotonin.Results : 1. Gagamyeonryunggobon-dan treated group had more and thicker hairs than the group not treated. Especially well developed sebaceous glands were seen in dermis of treated group. 2. Gagamyeonryunggobon-dan treated group had more capillaries near hair follicles of subcutaneous layer and more 2019% MIP-2 positive activity than the group not treated. 3. Gagamyeonryunggobon-dan treated group increased positive activity up to 596% in PCNA, 187% in IGF-2 and 547% in serotonin more than the group not treated.Conclusion : These results shows that Gagamyeonryunggobon-dan have the hair regrowth effect through verifying change of morphology, angiogenesis, chemokines. Consequently Gagamyeonryunggobon-dan is expected to apply to take care of extensive hair loss symptoms.

• Archives of Pharmacal Research
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• v.28 no.9
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• pp.1086-1091
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• 2005

An analytical method was developed for evaluating the cannabidiol (CBO), cannabinol (CBN), ${\Delta}^9-tetrahydrocannabinol$ $({\Delta}^9-THC)$ level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50mg) were washed with isopropyl alcohol and cut into small fragments (< 1mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0M NaOH for 10 min at $95^{\circ}C$. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and ${\Delta}^9-THC$ at three concentration levels were $37.9-94.5\%$ with good correlation coefficients $(r^2>0.9989)$. The intra-day precision and accuracy ranged from $-9.4\%\;to\;17.7\%$, and the inter-day precision and accuracy ranged from $-15.5\%\;to\;14.5\%$, respectively. The limits of detection (LOD) for CBD, CBN, and ${\Delta}^9-THC$ were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.169.1-169.1
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• 2003

This study was designed to compare the results and concentraions of methamphetamine (MA) and its metabolite amphetamine (AP) in head hair and hair collected from different parts of the body (axullae, pubis, and leg). Hair of subjects (N=15) suspected for MA abuse was simultaneously collected. (omitted)

• Journal of the Korea Convergence Society
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• v.10 no.6
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• pp.281-286
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• 2019

Appearance is a means of competitiveness for modern people, and one of the factors that harms the desires of modern people is white hair. For the modern man who needs to express beautiful appearance to others by covering white moth, we performed hair analysis after white moth hair dye treatment. The maximum modulus and tangential modulus according to the maximum load, maximum tensile strength, maximum elongation, breaking load, fracture strength, elongation at break, and evaluation interval between 1N-3N experimental group and control group of 1N-Respectively. Maximum load, maximum tensile strength, breaking load and breaking strength tended to be larger than those of the control group, while the maximum elongation and breaking elongation were the highest in the control group and the elongation decreased with the experimental group treatment. The maximum modulus and tangential modulus of the 1N-3N test group were higher than those of the control group at 0~0.15 and 0.15~2.5, respectively. Based on the study on the cosmetic changes of the hair before and after the treatment, it can be used as a basic data to select the correct oxidative hair dye product, the proper amount of application and the time to leave.

• Journal of Fashion Business
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• v.7 no.1
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• pp.116-134
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• 2003

The beauty industry of today tends to gradually develope as one of the important marketing strategies along with the total fashion sector. Hair color, being one of the factors of hair style, especially builds his or her own image and even becomes the nonverbal communication method which let others know him/herself. Therefore, women's needs and expectations of hair coloring are varied. To meet these need, it is urgent to figure out, most of all, the status of customers' awareness about hair coloring. Thus, in this study, research and analysis on hair coloring were made, focusing on the women utilizing 20 beauty shops in Changwon city. And the goal that this study set is to use as the study material for the hair coloring consulting in the beauty industry and the basic data for marketing in the industry job-sites. The conclusion by the three factors(age, job, monthly income) were as follows: 1. Dandyism was on the 1st order in the reason for coloring hair regardless of age, job, and monthly income. This seems to be speaking for the change of women's awareness of coloring hair. 2. The subject of making decision upon whether she was going to color her hair or not was herself regardless of age, job, and monthly income. Among the people belong to these three factors, brown was the color in overall preference. 3. More than half of those who belong to the three factors were not aware of the hair color, but their senses and interest in the color in vogue were high. 4. In terms of the hair coloring periodic time, many people had $2{\sim}3$months, but it was possible to know that they were interested in improving their images, considering the fact that most of the salaried people had their hair dyed within a month and the people in service job over a month. 5. The acquaintance around me had the strongest effect in collecting information from the people in those three factors and mass media was the next. The results of this study, in my opinion, would help the customers have beautiful and healthy hair and make images of their own by making the hair designers develope professional knowledges and skills on the hair coloring and improve the quality of beauty services.