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Studies on the life history of green peach aphid, Myzus persicae Sulzer (Homoptera) (복숭아흑진딧물(Myzus persicae Sulzer)의 생활사에 관한 연구)

  • Shim J. Y.;Park J. S.;Paik W. H.;Lee Y. B.
    • Korean journal of applied entomology
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    • v.16 no.3 s.32
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    • pp.139-144
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    • 1977
  • The green peach aphid Myzus persicae(Sulzer), is known as the most important vector of potato leaf-roll virus and PVY. Yield of potato plants infested with these viruses are remarkably lower than non-infested plants. A study was conducted to investigate the life history of the green peach aphid at Suweon, Korea (Lat. $37^{\circ}$'N., Long. $126^{\circ}59$'E). The following were obtained: 1. Overwintering eggs hatched from late March to early April, with a hatching rate averaging ninety-five percent. 2. The fundatrigeniae leave the primary host(Punus persica) in early to mid May and migrate to the secondary hosts. 3. From mid to late Oct., the gymnoparae migrate from the secondary hosts to the primary hosts. 4. From early to mid Nov., gymnoparae lay fertilized eggs around buds, in bark crevices, or between bifurcated twigs of the primary hosts. 5. The early-born progeny have 23 generations and the late-born progeny have 9 generations on peach trees, potatoes and raddish from Apr. to Oct. 6. The average length of life was about 28.5 days, with a developmental period of approximate 10.8 days and a reproductive period of 15.8 days. 7. The average number of nymphs produced by a female was fifty, with a maximum number of 118. 8. The average number of nymphs produced by a female per day was about 3.2, with a maximum number of 13.

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Enhancement of Species-specific Attraction by Addition of a Minor Component of Sex Pheromone Gland of Grapholita dimorpha (복숭아순나방붙이 성페로몬샘의 미량성분 첨가에 따른 종특이적 유인력 증가 효과)

  • Jung, Chung Ryul;Kim, Seong-Ho;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.239-246
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    • 2014
  • Two congener species of Grapholita molesta and G. dimorpha have similar host ranges and adult occurrence patterns. In addition, the two species commonly use cis-8-dodecenyl acetate (Z8-12:Ac) and trans-8-dodecenyl acetate (E8-12:Ac) as their major sex pheromone components. The commercial lures made of the two components, therefore, suffer a mixed attraction of these two species. This study was conducted to assess any effect of their known minor sex pheromone components to reduce the mixed attraction. When cis-8-tetradecenyl acetate (Z8-14:Ac) was added to the commercial sex pheromone consisting of Z8-12:Ac/E8-12:Ac = 85/15, the attraction to G. dimorpha was not affected, but its attraction to G. molesta was significantly reduced. However, the addition of either dodecanyl acetate (12:Ac) or tetradedecanyl acetate (14:Ac) did not influence on the attraction of both species to the commercial lure. The addition of E8-14:Ac to 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10, the attraction to G. dimorpha was rather significantly suppressed. From these results, the 3-component sex pheromone, Z8-12:Ac/E8-12:Ac/Z8-14:Ac = 85/15/10 was suggested as an improved composition of sex pheromone lure for G. dimorpha.

Interactions between Pine Needle Gall Midge, Thecodiplosis japonensis (Diptera: Cecidomyiidae), and its Parasitoids in Newly Invaded Areas (솔잎혹파리 확산지역에서 솔잎혹파리와 기생봉의 상호작용)

  • 박영석;정여진;전태수;이범영;이준호
    • Korean journal of applied entomology
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    • v.40 no.4
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    • pp.301-307
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    • 2001
  • The pine needle gall midge (PNGM), Thecodiplosis japonensis Uchida et Inouye, is a serious pest in pine forests in Korea. Since the first report of PNGM infestation in Korea in 1929, the infestation area has been expanded gradually. In 1975 the distribution of PNGM and its parasitoids were surveyed throughout several infested areas in Korea. Annual survey has been made for the rates of gall formation by PNGM and parasitism by its parasitoids at the monitoring sites including newly infested area in 1975 since 1980. These data were used to examine the relationship between PNGM and its parasitoids for newly invaded areas. The gall forming rate of PNGM and the parasitism by the parasitoid were 34.8 and 1.9% in 1975, respectively, while the gall forming rate and the parasitism were 20.7 and 8.9% in 1985, respectively. The relationships of densities between PNGM and its parsitoid were weak in the early stage of dispersion, but the density of parasitoid was with an asymptotic increase along with PNGMs density increase during the observation period for 10 years.

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Effect of Pinelliae Rhizoma Herbal Acupuncture on the Release of Thymus and Activation-Regulated Chemokine(TARC) in Human Bronchial Epithelial Cell (반하(半夏) 약침액(藥鍼液)이 사람 기관지 상피세포의 TARC 분비에 미치는 효과)

  • Hong, Jae-hwa;Seo, Jung-chul;Lim, Seong-chul;Jung, Tae-young;Han, Sang-won
    • Journal of Acupuncture Research
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    • v.22 no.1
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    • pp.155-164
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    • 2005
  • Objective : Chemokines are important for the recruitment of leukocytes, which is essential in host defense to the sites of infection. The thymus and activation-regulated chemokine (TARC) is a CC chemokine which potentially plays a role via a paracrine mechanism in the development of allergic respiratory diseases. Methods : The objective of this study is to investigate the effect of Pinelliae Rhizoma Herbal Acupuncture Solution (PHS) on the secretion of TARC of human bronchial epithelial cell. Methods : Enzyme-linked immunosorbent assay (ELISA) was performed to detect the secretion of TARC. The cytotoxicity was measured by MTT assay. Results : PHS significantly inhibited the secretion of TARC with a dose-dependant manner. The effective dosage did not have the cytotoxicity on human bronchial epithelial cell by MTT assay. Conclusion : Results of our study imply that PHS would play an important role in modulation of TARC in Human bronchial epithelial cells.

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Evaluation of bacteriophages for prevention and treatment of diarrhea due to experimental enterotoxigenic Escherichia coli K88 infection of weaned piglets (이유자돈의 실험적 enterotoxigenic Escherichia coli K88 감염에 의한 설사증에 대한 박테리오파지의 예방과 치료에 대한 효능 평가)

  • Kim, Sung-Jae;Kim, Jin-Dong;Yang, Si-Yong;Kim, Nam-Hee;Lee, Chang-Hee;Yang, Don-Sik;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.34 no.4
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    • pp.341-352
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    • 2011
  • Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 ($1{\times}10^8$ CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C, $1.0{\times}10^6$ PFU/g; Group D, $1.0{\times}10^8$ PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P<0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P<0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P<0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.

The Effects of Porous Resorbable Calcium Carbonate and Porous Replamineform Hydroxyapatite on the regeneration of the alveolar bone in the Periodontally involved extraction sockets in dogs. (Porous Resorbable Calcium Carbonate와 Porous Replamireform Hydroxyapatite가 성견치주질환 이환 발치와내 이식된 치근과 발치와 치조골 재생에 미치는 영향)

  • Kim, Chong-Kwan;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.334-349
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    • 1996
  • Regeneration of the periodontal tissue destroyed by periodontal disease is one of the final goals of periodontal therapy. In the past few years, periodontists have used various alloplastic grafting materials in an attempt to regenerate bone lost from periodontal disease. These materials have used widely because they have shown to be nontoxic, biologically compatible with surrounding host tissue and chemically similar to bone. The purpose of this study was to investigate the effect of Porous Resorbable Calcium Carbonate and Porous Replamineform Hydroxyapatite on the regeneration of the alveolar bone and the healing of roots transplanted into the periodontally diseased extraction sockets of dogs. The experimental chronic periodontitis was induced by elastic ligatures on the 2nd and 3rd mandibular premolars of 2 adult dogs for 8weeks after surgically creating periodontal defect. The extracted root were split in half along the long-axis, and the extend of plaque exposure was marked on the root surfaces with burs. The roots were inserted in extraction sockets with Porous Resorbable Calcium Carbonate(PRCC) in left side and with Porous Replaminefrom Hydroxyapatite(PRH) in right side. The flaps were sutured to cover the sockets completely. The animals were sacrificed after 12 weeks of healing, and the specimens were examined histologically. The results were as follows: 1. No inflammatory reactions were observed in either groups. 2. Hoot resorption was observed in both groups while the general outline of the roots were maintained. 3. PRCC was almost completely resorbed and replaced with new bone, while R.H.A. was not resorbed & remained encased in newly-formed C-T and alveolar bone. 4. PRH was encapsulated with alveolar bone which has been deposited from apical & lateral area of the sockets, while the coronal portion of the sockets were filled with C-T. 5. In both groups, the resorbed portions of the roots were replaced with new bone. These results suggest that either PRCC or PRH may not interfere with bone formation or healing in extraction sockets, and in some degree, retard the root resorption. Because the roots maintained in anatomy, we think that graft materials prevent the root resorption.

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Biomechanical evaluation of dental implants with different surfaces: Removal torque and resonance frequency analysis in rabbits

  • Koh, Jung-Woo;Yang, Jae-Ho;Han, Jung-Suk;Lee, Jai-Bong;Kim, Sung-Hun
    • The Journal of Advanced Prosthodontics
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    • v.1 no.2
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    • pp.107-112
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    • 2009
  • STATEMENT OF PROBLEM. Macroscopic and especially microscopic properties of implant surfaces play a major role in the osseous healing of dental implants. Dental implants with modified surfaces have shown stronger osseointegration than implants which are only turned (machined). Advanced surface modification techniques such as anodic oxidation and Ca-P application have been developed to achieve faster and stronger bonding between the host bone and the implant. PURPOSE. The purpose of this study was to investigate the effect of surface treatment of titanium dental implant on implant stability after insertion using the rabbit tibia model. MATERIAL AND METHODS. Three test groups were prepared: sandblasted, large-grit and acid-etched (SLA) implants, anodic oxidized implants, and anodized implants with Ca-P immersion. The turned implants served as control. Twenty rabbits received 80 implants in the tibia. Resonance frequencies were measured at the time of implant insertion, 2 weeks and 4 weeks of healing. Removal torque values (RTV) were measured 2 and 4 weeks after insertion. RESULTS. The implant stability quotient (ISQ) values of implants for resonance frequency analysis (RFA) increased significantly (P <. 05) during 2 weeks of healing period although there were no significant differences among the test and control groups (P >. 05). The test and control implants also showed significantly higher ISQ values during 4 weeks of healing period (P < .05). No significant differences, however, were found among all the groups. All the groups showed no significant differences in ISQ values between 2 and 4 weeks after implant insertion (P >. 05). The SLA, anodized and Ca-P immersed implants showed higher RTVs at 2 and 4 weeks of healing than the machined one (P < .05). However, there was no significant difference among the experimental groups. CONCLUSION. The surface-modified implants appear to provide superior implant stability to the turned one. Under the limitation of this study, however, we suggest that neither anodic oxidation nor Ca-P immersion techniques have any advantage over the conventional SLA technique with respect to implant stability.

A clinical study of the effect of Nd:YAG laser irradiation on inflamed gingiva (염증성 치은에 대한 Nd:YAG laser 조사효과에 관한 임상적 연구)

  • Cho, Hyung-Soo;Kim, Hyun-Sup;Lim, Kee-Jung;Kim, Byung-Ock;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.531-541
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    • 1996
  • Periodontal disease is characterized by destruction of supporting tissues caused by invasion of plaque bacteria and defense mechanism of host. Many dentists are very interested in laser therapy on various intraoral soft tissue lesions including inflammatory periodontal pocket. In order to determine the therapeutic effect of intrapocket irradiation of a pulsed- Nd : YAG laser on the inflammatory periodontal pockets, bilateral 60 teeth with 4-6mm in probing pocket depth and gingival inflammation were selected and evaluated by sulcus bleeding index(SBI), and plaque index(pI) for baseline record. Intrapocket irradiation($300{\mu}m$ fiber optic, I.5W power, for 2 min.) of a pulsed-Nd : YAG laser(EL.EN.EN060, Italy) was applied on half of them. As the control group, the same procedure except power-off was repeated on the contralateral 30 teeth. At 1-, 2-, 3-, and 4-week after intrapocket manipulation, every tooth was reevaluated by the same clinical indices. And the difference between the lased group and control group was statistically analyzed by paired t-test and Chi-square test in Microstat program. Following results were obtained: 1. Until I-week and 2-week after intrapocket manipulation, SBI was lowered in both lased group and control group, compared to baseline SBI, but from 3-week after, the recovering tendency toward baseline was noted, and at only 2-week after, the number of teeth showing lowered SBI was significantly more in lased group than in control group(p<0.05). 2. PI of both lased group and control group was lowered through whole experimental period from I-week to 4-week after, compared to baseline PI(p<0.05), but there was no significant difference between lased group and control group(p>0.1). The results suggest that intrapocket irradiation of a pulsed-Nd:YAG laser may lead somewhat remission of gingival inflammation, but for more favorable therapeutic result the thorough root planing should be necessarily accompanied with gingival curettage.

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Synthesis of (Co,Mg)Al2O4 and (Ni,Mg)Al2O4 Blue Ceramic Nano Pigment by Polymerized Complex Method (착체중합법을 이용한 (Co,Mg)Al2O4 및 (Ni,Mg)Al2O4 청색 나노 무기안료 합성)

  • Son, Bo-Ram;Yoon, Dea-Ho;Kim, Jin-Ho;Han, Kyu-Sung;Cho, Woo-Suk;Hwang, Kwang-Taek
    • Journal of the Korean Ceramic Society
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    • v.50 no.6
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    • pp.510-517
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    • 2013
  • In this study, the properties of blue inorganic nano-pigments with a spinel structure were systematically investigated. We report the preparation of a blue ceramic nano-pigment and the Co and Ni substitutional effects on the blue color. $MgAl_2O_4$ was selected as the crystalline host network for the synthesis of cobalt and nickel-based blue ceramic nano-pigments. Various compositions of $Co_xMg_{1-x}Al_2O_4$ and $Ni_xMg_{1-x}Al_2O_4$ ($0{\leq}x{\leq}1$) powders were prepared using apolymerized complex method. The obtained powder was preheated at $400^{\circ}C$ for 5 h and then calcined at $1000^{\circ}C$ for 5 h. XRD patterns of the (Co,Mg)$Al_2O_4$ and (Ni,Mg)$Al_2O_4$ samples showed a single phase of the spinel structure in all compositions. TEM results indicated nano-sized pigments for (Co,Mg)$Al_2O_4$ and (Ni,Mg)$Al_2O_4$ with a particle size ranging from 20 to 50 nm. The characteristics of the color tones of (Co,Mg)$Al_2O_4$ and (Ni,Mg)$Al_2O_4$ were analyzed by CIE $L^*a^*b^*$ measurements. In addition, the thermal stability and the binding characteristics of (Co,Mg)$Al_2O_4$, (Ni,Mg)$Al_2O_4$ are discussed in terms of the TG-DSC and FT-IR results, respectively.

The Role of Protein Kinase C and Protein Tyrosine Kinase in the Signal Transduction Pathway of Stimulus Induced by Endotoxin in Peripheral Blood Monocyte (말초혈액 단핵구에 대한 내독소 자극의 신호 전달에서 Protein Kinase C와 Protein Tyrosine Kinase의 역할)

  • Kim, Jae-Yeol;Park, Jae-Suk;Lee, Gwi-Lae;Yoo, Chul-Gyu;Kim, Young-Whan;Han, Sung-Koo;Shim, Young-Soo
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.2
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    • pp.338-348
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    • 1997
  • Background : Endotoxin, the component of outermembrane of gram negative organism, plays an important role in the initiation and amplification of inflammatory reaction by its effects on inflammatory cells. Until recently, there have been continuing efforts to delinate the mechanisms of the signal trasduction pathway of endotoxin stimuli on inflammatory cells. By uncovering the mechanisms of signal transduction pathway of endotoxin stimuli, we can expect to have tools to control the excessive inflammatory responses which sometimes may be fatal to the involved host. It was generally accepted that endotoxin exerts its inflammatory effects through inflammatory cytokines that are produced by endotoxin-stimulated inflammatory cells and there were some reports on the importance of protein kinase C and protein tyrosine kinase activation in the production of inflammatory cytokines by endotoxin So we evaluated the effect of pretreatment of protein kinase C inhibitors (H7, Staurosporin) and protein tyrosine kinase inhibitors(Herbimycin, Genistein) on the endotoxin-stimulated cytokines(IL-8 & TNF-$\alpha$) mRNA expression. Method : Peripheral blood monocytes were isolated from healthy volunteers by Ficoll-Hypaque density gradient method and purified by adhesion to 60mm Petri dishes. Endotoxin(LPS 100ng/ml) was added to each dishes except one control dish, and each endotoxin-stimulated dishes was preincubated with H7, Staurosporin(protein kinase C inhibitor), Herbimycin or Genistein(protein tyrosine kinase inhibitor) respectively except one dish. Four hours later the endotoxin stimulation, total RNA was extracted and Northern blot analysis for IL-8 mRNA and TNF-$\alpha$ mRNA was done. Result : Endotoxin stimulation increased the expression of IL-8 mRNA and TNF-$\alpha$ mRNA expression in human peripheral blood monocyte as expected and the stimulatory effect of endotoxin on TNF-$\alpha$ mRNA expression was inhibited by protein kinase C inhibitors(H7, Staurosporin) and protein tyrosine kinase inhibitors (Herbimycin, Genistein). The inhibitory effect of each drugs was increased with increasing concentration. The stimulatory effect of endotoxin on IL-8 mRNA was also inhibited by H7 and protein tyrosine kinase inhibitors (Herbimycin, Genistein) dose-dependently but not by Staurosporin. Conclusion : Protein kinase C and protein tyrosine kinase are involved in the endotoxin induced signal transduction pathway in human peripheral blood monocyte.

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