• BMB Reports
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• 제33권4호
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• pp.308-311
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• 2000

The interrelationship between the differentiation and expression of mRNA for transferrin in the HL-60 leukemia cell line was studied. Transferrin mRNA was expressed in HL-60 leukemia cells and the amount was 50% of that in the positive control cell line, HepG-2 cells. The expression of $T_f$ mRNA in HL-60 cells was not regulated by IL-1, IL-6 and $TNF-{\alpha}$, respectively. The expression of $T_f$ mRNA in the differentiated cells into a granulocyte lineage by DMSO, or all-trans RA, was up-regulated (160-170% of control cells); whereas, the expression was not regulated in the differentiated cells into a macrophage lineage by PMA. These results suggest that the differentiation to a granulocyte lineage of HL-60 leukemia cells appear to be related with the upregulation of transferrin mRNA expression.

• 대한본초학회지
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• 제26권2호
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• pp.31-37
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• 2011

Objectives : This study was focused to investigate the toxicity of Saussurea lappa (SL) extracts in HL-60 cells and human lymphocytes. We also examined the differentiation effect of SL against leukemia cells. Methods : For examining the toxicity of SL, cytokinesis-block micronucleus (CBMN) assay and single cell gel eletrophoresis (SCGE) assay were used in present study. The cell differentiation effect of SL was evaluated by nitroblue tetrazolium (NBT) reduction assay. Results : The inhibition of cell growth in HL-60 cells was observed in a dose-dependant manner after SL treatment for 24 h. According to SCGE assay, HL-60 cells treated with SL increased DNA damage at $10{\mu}g/m{\ell}$, while DNA damage was induced by 0.1, 1, $10{\mu}g/m{\ell}$ concentration of SL in human lymphocytes. Our results indicated that SL have no genotoxic effect in HL-60 cells and human lymphocytes. Additionally, the differentiation effect was induced in $1{\mu}g/m{\ell}$ SL-treated HL-60 cells. Conclusions : From above results it is suggested that SL could be beneficial for the preparation of the useful agent for treating leukemia.

• 한국콘텐츠학회논문지
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• 제12권2호
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• pp.339-348
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• 2012

급성 전골수구성 백혈병(acute promyelocytic leukemia, APL)은 치료제가 한정적이고 그 또한 다양한 부작용을 초래한다. 최근 암세포 형성 억제에 세균 추출물을 사용하는 경우가 증가하는데 이를 이용하여 기존의 약제보다 효과적이면서 부작용이 적은 치료제 개발이 필요하다. 본 연구에서는 L. monocytogenes에서 분비되는 물질(LmSup)과 세균 자체가 함유하고 있는 물질(LmE)을 추출하여 HL-60 세포에 처리한 다음 세포증식 억제 효과를 보고자 하였다. 세포 생존율 및 세포고사를 확인하여 세포를 죽음으로 유도하는 지 파악한 다음 작용기전을 규명하고자 세포주기의 변화 및 ROS 생성을 관찰하였다. 그 결과, LmSup와 LmE가 급성 전골수구성 백혈병(APL) 세포인 HL-60의 세포고사를 유도하고, sub G0/G1기 증가로 세포주기를 비정상적으로 차단함으로써 세포고사를 유도함을 확인하였다. 이때, ROS가 관여함을 관찰하였다. 이를 통해, LmSup 또는 LmE의 구체적인 항암효과 및 기전 분석을 통해 난치병인 APL의 치료 방법 및 치료제 개발에 기여하고자 한다.

• 한국작물학회지
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• 제43권3호
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• pp.168-171
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• 1998

Sesaminol in sesame seed was postulated to have antitumor activity. The present study was performed to characterize the role of crude sesaminol extracted from sesame seed (Sesame Crude Sesaminol; SCS) on inhibiting the in vitro growth of human leukemia HL-60 cells. SCS inhibited the growth of human leukemia HL 60 cells in culture and macromolecular synthesis in a dose and time dependent manner. The cytostatic range of SCS concentration was found to be 60 to 100 $\mu\textrm{g}$/ml. SCS concentration greater than 200 $\mu\textrm{g}$/mlwere cytocidal to HL-60 cells. When SCS concentraction was 6 $\mu\textrm{g}$/mland 50 $\mu\textrm{g}$/ml the synthesis of HL-60 cells was inhibited by 35% for DNA, 6% for RNA and 5% for protein and 83% for DNA, 76% for RNA and 60% for protein. Of specific interest was the irreversible effect of SCS in inhibiting DNA synthesis of HL-60 cells. This was evidenced from the fact that, even after washed with PBS three times, preincubated HL-60 cells still showed the inhibited DNA synthesis.

• 생약학회지
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• 제40권2호
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• pp.118-122
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• 2009

Methanol extracts of Orostachys japonicus A. Berger (OAB) were found to exhibit apoptosis induction of HL60 human acute promyelocytic leukemia cells. Treatment of OAB exerted strong cytotoxicity against HL60 cells. OAB induced DNA fragmentation of HL60 cells in a dose dependent manner. Nitric oxide production were also increased in OAB-treated RAW264.7 macrophage cell lines. Treatment of OAB increased the expression of p53 and iNOS gene and the expression of p53, $NF-{\kappa}B$ and iNOS protein in cultured HL60 and RAW264.7 cells. These results suggest that OAB are effective on strong anti-cancer properties and can be useful as a chemo-preventive agents.

• Toxicological Research
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• 제17권2호
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• pp.131-137
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• 2001

Baicalein, a major flavonoid of extract from Scutellaria baicalensis Georgi, has been shown to exhibit antioxidant and anti proliferative effects. In the present study, we investigate the effects of baicalein on viability and induction of apoptosis in human promyelocytic leukemia HL-60 cells. Baicalein was found to induce apoptosis of HL-60 cells in a concentration-dependent and time-dependent manner. When HL-60 cells were exposed to 100 $\mu\textrm{M}$ baicalein for 6h, the viability was decreased remarkably to 27% of control, whereas DNA fragmentation was significantly increased to 64%. Nucleosomal fragmentation of baicalein treated HL-60 cells, a hallmark of apoptosis, was further identified by agarose gel electrophoresis (DNA ladder). Flow cytometric analysis showed that apoptotic cells were increased to 66.6% after treatment with 100 $\mu\textrm{M}$ baicalein for 6 h. Baicalein-induced apoptosis of HL-60 cells was reduced by 1h pretreatment with inhibitor of caspases, z-Asp-$CH_2$-DCB. At 3 and 10 $\mu\textrm{M}$ of z-Asp-$CH_2$-DCB, DNA fragmentation of HL-60 cells induced by baicalein (50 $\mu\textrm{M}$) was 36.8 and 17.1 %, respectively, whereas, that of HL-60 cells treated by baicalein (50 $\mu\textrm{M}$) without pretreatment with inhibitor of caspases was 62.7%. These data suggest that baicalein induces apoptosis in human leukemia HL-60 cells, and that caspase enzymes might be involved in baicalein-induced apoptosis.

• Toxicological Research
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• 제20권4호
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• pp.307-313
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• 2004

The present study was undertaken to investigate the effects of mistletoe (Viscum album var. coloratum) growing on Carpinus laxiflora BL. on proliferation and differentiation of HL-60 acute promyelocytic leukemia cells. Aqueous extract and its $(NH_2)_2SO_4$ saturated fractions of the mistletoe exhibited potent anti-proliferation activity against HL-60 cells. Moreover, when HL-60 cells were treated with 0~30% and 30～70% $(NH_2)_2SO_4$ saturated fractions of the mistletoe, HL-60 expressed CD 66b or CD 14 cell surface antigens and showed activity to reduce nitroblue tetrazolium, indicating that mistletoe induces the differentiation of HL-60 into granulocytes or monocytes. To understand how mistletoe induces the differentiation, we investigated the expression of molecules for modulating the proliferation and differentiation of leukemia cells, such as c-Myc and myeloblastin. The 0~30% $(NH_2)_2SO_4$ saturated fraction of the mistletoe reduced the mRNA levels of c-Myc and myeloblastin in a time-dependent manner. The results indicate that the mistletoe induces the differentiation of HL-60 cells via the decrease of c-Myc and myeloblastin expressions. Thus, it is suggested that mistletoe has a therapeutic potential for the treatment of acute promyelocytic leukemia.

• 생약학회지
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• 제44권3호
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• pp.269-274
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• 2013

In this study, we investigated the anticancer effect of rice bran extract in HL-60 human promyelocytic leukemia cells. The extract of rice bran inhibited the proliferation of HL-60 cells. When treated with the rice bran extract, we could observe the apoptotic characteristics such as apoptotic bodies and the increase of sub-G1 hypodiploid cell population, increase of Bax level, decrease of Bcl-2 expression, cleavage of procaspase-3, cleavage of procaspase-9 and cleavage of poly(ADP-ribose) polymerase(PARP) in HL-60 cells. Furthermore, the apoptosis induction of HL-60 cells treated with the rice bran extract was also accompanied by the inactivation of mitogen-activated protein kinases (MAPK) such as ERK1/2 MAPK and p38 MAPK. In addition, the rice bran extract induced the down-regulation of c-myc. These data suggested that the rice bran extract could induce the apoptosis via the inactivation of ERK1/2 MAPK and p38 MAPK, and the down-regulation of c-myc in HL-60 acute pomyelocytic leukemia cells. The results support that the rice bran extract might have potential for the treatment of acute promyelocytic leukemia.

• 생명과학회지
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• 제8권2호
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• pp.162-166
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• 1998

전 골수성 백혈병 세초인 HL-60 세포를 model로 하여, 민간요법으로 사용되어져 부작용이 극히 적은 거승로 알려진 고려인삼의 구성 성분 중 주요성분이 ginseng (Panzx ginseng C.A. Meyer) saponin 및 ginsenoside Rh1, Rh2, Rh3, 비파 (Eriobotrya japonica L.) 잎의 성분들 중에서 항발암 및 항암성분으로 알려진 ursolic acid 및 oleanolic acid, 웅담중의 중요성분 성분인 lithocholoc acid 드잉 분화능력이 있는 지를 조사하고자 본 실험을 수행아였다. Retinoic acid를 처리한 결과 타 연구자들의 연구결과들처럼 높은 분화력을 관찰할 수 있었으며, dbcAMP 단독 처리군에서도 높은 분화효과를 나타냈었다. Dexamethasone 처리군에서는 분화효과를 거의 관찰할 수 없었으나,dexamethansone과 구조적으로 유사한 ursolic acid와 oleanolic acid는 보다 높은 분화력을 보였고 웅담성분의 중요성분인 lirhocholic acid는 높은 분화력을 나타냈었다. Ginseng saponin은 0.00375% (w/v)에서 20% 이상의 분화력을 보였으며, Ginsenoside Rh2와 Rh3는 높은 분화력을 나타냈다.

• 한국콘텐츠학회논문지
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• 제14권6호
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• pp.241-246
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• 2014

급성전골수구성 백혈병(Acute promyelocytic leukemia, APL)은 혈액암으로 치료가 쉽지 않을 뿐만 아니라 항암치료에 가장 효과가 좋다는 방사선 치료를 해도 오히려 정상세포에도 작용하여 부작용을 초래하게 된다. 본 연구에서는 전자선(EB)을 TNF-${\alpha}$와 같이 처리하였을 경우 정상세포와 암세포의 세포 죽음에 어떠한 영향을 미치는지 확인하였다. HL-60세포는 APL세포주로서 사용하였고 DMSO를 처리하여 분화시킨 HL-60세포는 정상과립구의 성질을 나타내어 정상대조군으로 이용하였다. 그 결과 TNF-${\alpha}$를 전자선을 처리한 HL-60세포에서만 세포독성효과를 나타내었고 이 과정에서 TNF-${\alpha}$는 caspase3를 활성화 시켜서 세포자멸사를 유도하여 세포가 죽음에 이르게 하였다. 결론적으로 TNF-${\alpha}$는 항암치료의 부작용을 없애기 위해 저농도의 전자선 치료 시 함께 사용하여 암 세포의 제거를 증가시켜 암의 치료효율을 높일 수 있는 유효물질로 사료된다.