The Journal of the Korea Contents Association (한국콘텐츠학회논문지)

The Korea Contents Association (한국콘텐츠학회)
권호 표지
DOI QR코드

DOI QR Code

Extract of Listeria monocytogenes Induces the Apoptosis on the Human Promyelocytic Leukemia Cells, HL-60 Cells

Listeria monocytogenes에 의해 HL-60 cell의 세포고사 유도 효과 규명

  • 양은주 (대구한의대학교 보건치료대학 임상병리학과) ;
  • 김동현 (부산가톨릭대학교 보건과학대학 방사선학과) ;
  • 장정현 (대구한의대학교 보건치료대학 임상병리학과)
  • Received : 2011.10.28
  • Accepted : 2011.11.24
  • Published : 2012.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Acute promyelocytic leukemia (APL) is a cancer of the blood and bone marrow. Although all-trans retionic acid (ATRA) is the agents for ALP therapy, there are various side effects. For overcome this problem, we need the development of new therapeutic agents for APL. A number of bacteria produce various virulence factors with cytotoxic effects on human cancer cells. To understand the anti-cancer effect of Listeria monocytogenes on APL, we examined alteration of the cell viability, apoptosis and cell cycle arrest of the human promyelocytic leukemia cell line, HL-60 cells. The cell supernatant (LmSup) and the extract of L. monocytogenes (LmE) inhibited the cell viability and induced apoptosis of HL-60 cells. These cytotoxic effect of LmSup and LmE mediated by modulation of cell cycle and ROS production. These results indicate that released or included bacterial molecules from L. monocytogenes have a cytotoxicity in HL-60 cells. Therefore, LmSup and LmE may be used as the potential target for the treatment of cancer induced by HL-60 cells.

급성 전골수구성 백혈병(acute promyelocytic leukemia, APL)은 치료제가 한정적이고 그 또한 다양한 부작용을 초래한다. 최근 암세포 형성 억제에 세균 추출물을 사용하는 경우가 증가하는데 이를 이용하여 기존의 약제보다 효과적이면서 부작용이 적은 치료제 개발이 필요하다. 본 연구에서는 L. monocytogenes에서 분비되는 물질(LmSup)과 세균 자체가 함유하고 있는 물질(LmE)을 추출하여 HL-60 세포에 처리한 다음 세포증식 억제 효과를 보고자 하였다. 세포 생존율 및 세포고사를 확인하여 세포를 죽음으로 유도하는 지 파악한 다음 작용기전을 규명하고자 세포주기의 변화 및 ROS 생성을 관찰하였다. 그 결과, LmSup와 LmE가 급성 전골수구성 백혈병(APL) 세포인 HL-60의 세포고사를 유도하고, sub G0/G1기 증가로 세포주기를 비정상적으로 차단함으로써 세포고사를 유도함을 확인하였다. 이때, ROS가 관여함을 관찰하였다. 이를 통해, LmSup 또는 LmE의 구체적인 항암효과 및 기전 분석을 통해 난치병인 APL의 치료 방법 및 치료제 개발에 기여하고자 한다.

Keywords

References

  1. 김휘영, "자궁 재활치료를 위한 울트라-스캔방식의 펄스형 레이저시스템", 한국콘텐츠학회논문지, 제9권, 제6호, pp.256-265, 2009.
  2. N. Bernardes, R. Seruca, A. M. Chakrabarty, and A. M. Fialho, "Microbial-based therapy of cancer, Current progress and future prospects," Bioengineered Bugs, Vol.1, No.3, pp.178-190, 2010. https://doi.org/10.4161/bbug.1.3.10903
  3. Y. Andersson, S. Juell, and Q. Fodstad, "Downregulation of the anti-apoptotic MCL-1 protein and apoptosis in MA-11 breast cancer cells induced by an anti-dpidermal growth factor receptor-Pseudomonas exotoxin A immunotoxin," Int. J. Cancer, Vol.112, pp.475-483, 2004. https://doi.org/10.1002/ijc.20371
  4. A. M. Chakrabarty, "Microorganisms and Cancer: Quest for a therapy," J. Bacteriol, Vol.185, pp.2683-2686, 2003. https://doi.org/10.1128/JB.185.9.2683-2686.2003
  5. M. S. Tallman and J. K. Altman, "Curative strategies in acute promyelocytic leukemia," Hematology, Vol.2008, No.1, pp.391-399, 2008. https://doi.org/10.1182/asheducation-2008.1.391
  6. L. K. Hillestad, "Acute promyelocytic leukemia," Acta. Med. Scand., Vol.159, No.3, pp.189-194, 1957. https://doi.org/10.1111/j.0954-6820.1957.tb00124.x
  7. M. S. Tallman, "Retinoic acid syndrome: a problem of the past?," Leukemia, Vol.16, No.2, pp.160-161, 2002. https://doi.org/10.1038/sj.leu.2402344
  8. R. W. Johnstone, A. A. Ruefli, and S. W. Lowe, "Apoptosis: a link between cancer genetics and chemotherapy," Cell, Vol.108, pp.153-164, 2002. https://doi.org/10.1016/S0092-8674(02)00625-6
  9. G. Kroemer and J. Pouyssegur. "Tumor cell metabolism: cancer's Achilles' heel," Cancer cell, Vol.13, pp.472-482, 2008. https://doi.org/10.1016/j.ccr.2008.05.005
  10. A. Melet, K. Song, O. Bucur, Z. Jagani, A. R. Grassian, and R. KhosraviFar, "Apoptotic pathways in tumor progression and therapy," Adv. Exp. Med. Biol., Vol.615, pp.47-79, 2008. https://doi.org/10.1007/978-1-4020-6554-5_4
  11. D. R. Green and J. D. Reed, "Mitochondria and apopotosis," Science, Vol.281, pp.1309-1312, 1998. https://doi.org/10.1126/science.281.5381.1309
  12. S. Cory and J. M. Adams, "The Bcl-2 family: regulators of the cellular life-or-death switch," Mat Rev Cancer, Vol.2, pp.647-656, 2002. https://doi.org/10.1038/nrc883
  13. M, Ishihama, T. Toyooka, and Y. Ibuki, "Generation of phosphorylated histone H2AX by benzene metabolites," Toxicol. in Vitro, Vol.22, pp.1861-1868, 2008. https://doi.org/10.1016/j.tiv.2008.09.005
  14. H. Terasaka, Y. Kadoma, H. Sakagami, and S. Fujisawa, "Cytotoxicity and apoptosis- inducing activity of bisphenol A and hydroquinone in HL-60 cells," Anticancer Res., Vol.3B, pp.2241-2247, 2005.
  15. K. P. Wilson, J. A. Black, J. A. Thomson, E. E. Kim, P. G. James, A. N. Manuel, M. A. Murcko, P. S. Chambers, R. A. Aldape, S. A. Raybuck, and D. J. Livingston, "Structure and mechanism of interleukin-1 beta converting enzyme," Nature, Vol.370, No.6497, pp.270-275, 1994. https://doi.org/10.1038/370270a0
  16. M. L. Gray and A. H. Killnger, "Listeria monocytogenes and listeric infection," Bacteriol. Rev., Vol.30, pp.309-382, 1966
  17. R. W. Armstrong and P. C. Fung, "Brainstem encephalitis (Rhombencephalitis) due to Listeria monocytogenes: case report and review," Clin. infect Dis., Vol.16, No.5, pp.689-702, 1993. https://doi.org/10.1093/clind/16.5.689
  18. F. Lowry, "Live Listeria vaccine proves safe against end-stage cervical cancer in human trial," Ob. Gyn. News, Vol.43, No.10, p.2, 2008.
  19. M. C. Parra, F. Baquero, and J. C. Perez-Diaz, "The role of apoptosis in Listeria monocytogenes neural infection: Listeriolysin ) interaction with neuroblastoma Neuro-2a cells," Infection, Generics and Evolution, Vol.8, pp.59-67, 2008. https://doi.org/10.1016/j.meegid.2007.10.002
  20. C. A. Guzman, E. Domann, M. Rohde, D. Bruder, A. Dariji, S. Weiss, J. Wehland, T. Chakraborty, and K. N. Timmis, "Apoptosis of mouse dendritic cells is triggered by listeriolysin, the major virulence determinant of Listeria monocytogenes," Mol Microbiol, Vol.20, pp.119-126, 1996. https://doi.org/10.1111/j.1365-2958.1996.tb02494.x
  21. M. J. Brunda, H. L. Mathews, H. R. Ferguson, J. K. McClatchy, and P. Minden, "Immunotherapy of the guinea pig line 10 hepatocarcinoma with a variety of nonviable bacteria," Cancer research, Vol.40, pp.3211-3213, 1980.
  22. G. P. Chrousos, S. D. Detera-Wadleigh, and M. Karl, "Syndromes of glucocorticoid resistance," Ann. Intem. Med., Vol.119, No.11, pp.1113-1124, 1993. https://doi.org/10.7326/0003-4819-119-11-199312010-00009
  23. K. W. Adams and G. M. Cooper, "Rapid turnover of mcl-1 couples translation to cell survival and apoptosis," J. Biol. Chem., Vol.282, pp.6192-6200, 2007. https://doi.org/10.1074/jbc.M610643200
  24. X. Du, R. J. Youle, D. J. FitzGerald, and P. Ira, "Pseudomonas Exotoxin A-Mediated apoptosis is Bak dependent and preceded by the degradation of Mcl-1," Molecular and Cellular Biology, Vol.30, No.14, pp.3444-3452, 2010. https://doi.org/10.1128/MCB.00813-09
  25. 남상남, 김종혁, 지민철, "유산소 운동을 병행한 근 저항성 운동이 노인 여성의 혈중 MDA 및 SOD, GPx 활성에 미치는 영향", 한국콘텐츠학회 논문지, 제9권, 제11호, pp.391-398, 2009.
  26. A. L. Decatur and D. A. Portnoy, "A PEST-like sequence in listeriolysin O essential for Listeria monocytogenes pathogenicity," Science, Vol.290, No.5493, pp.992-995, 2000. https://doi.org/10.1126/science.290.5493.992
  27. Z Wei, L. A. Zenewicz, and H. Goldfine, "Listeria monocytogenes phosphatidylinositolspecific phospholipase C has evolved for virulence by greatly reduced activity on GPI anchors," Proc. Natl. Acad. Sci. U. S. A., Vol.102, No.36, pp.12927-12931, 2005. https://doi.org/10.1073/pnas.0501725102
  28. L. M. Wood, P. D. Guirnalda, M. M Seavey and Y. Paterson, "Cancer immunotherapy using Listeria monocytogenes and listerial virulence factors," Immunol. Res., Vol.42, pp.233-245, 2008. https://doi.org/10.1007/s12026-008-8087-0
  29. R. W. Tindle, "Human popillloma virus vaccines for cervical cancer," Curr. Opin, Immunol., Vol.8, pp.643-650, 1996. https://doi.org/10.1016/S0952-7915(96)80080-X
  30. R. Singh and Y. Paterson, "Vaccination strategy determines the emergence and dominance of CD8+ T-cell epitopes in a FVB/N rat HER-2/neu mouse model of breast cancer," Cancer Res., Vol.66, pp.7748-7757, 2006. https://doi.org/10.1158/0008-5472.CAN-05-4469
  31. R. E. Hqwkins, D. Zhu, M. Ovecka, G. Winter, T. J. Hamblin, A. Long, and F. K. Stevenson, "Idiotypic vaccination against human B-cell lymphoma. Rescue of variable region gene sequences from biopsy material for assembly as single-chain Fv personal vaccines," Blood, Vol.83, No.11, pp.3279-3288, 1994.
  32. S. Bergelt, S. Frost, and H. Lilie, "Listeriolysin O as cytotoxic component of an immunotoxin," Protein Sci., Vol.18, pp.1210-1220, 2009. https://doi.org/10.1002/pro.130
  33. S. C. Kachlanya, A. B. Schwartza, N. V. Balashovaa, C. E. Hioeb, M. Tuenb, A. Lea, M. Kaura, Y. Meia, and J. Raoa, "Anti-leukemia activity of a bacterial toxin with natural specificity for LFA-1 on white blood cells,, Leukemia Res., Vol.34, No.6, pp.777-785, 2010. https://doi.org/10.1016/j.leukres.2009.08.022
  34. O. Choi, K. Yahiro, N. Morinaga, M. Miyazaki and M. Noda, "Inhibitory effects of various plant polyphenols on the toxicity of Staphylococcal alpha-toxin," Microb Pathog, Vol.42, pp.215-224,2007. https://doi.org/10.1016/j.micpath.2007.01.007
  35. L. Zhu, C. Li, J. Wu, J. Liang, and Y. Shi, "Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875," Wei Sheng Wu Xue Bao, Vol.44, pp.690-694, 2008.
  36. W. D. Schubert, C. Urbanke, T. Ziehm, V. Beier, M. P. Machner, E. Domann, J. Wehland, T. Chakraborty, and D. W. Heinz, "Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadheri," Cell, Vol.111, pp.825-836, 2002. https://doi.org/10.1016/S0092-8674(02)01136-4
  37. A. Kassam, S. D. Der, and J. Mogridge, "Differentiation of human monocytic cell lines confers susceptibility to Bacillus anthracis lethal toxin," Cell Microbiol, Vol.7, pp.291-292, 2005.

Cited by

  1. The Apoptosis according to the Processing Irradiation and The Tumor Necrosis Factor vol.10, pp.3, 2016, https://doi.org/10.7742/jksr.2016.10.3.195