• Title/Summary/Keyword: HI test

Search Result 480, Processing Time 0.026 seconds

Restriction map of a cryptic plasmid from Pseudomonas putida (Pseudomonas putida로 부터 분리한 cryptic플라스미드의 제한효소지도)

  • 김훈규;고상균;이영록
    • Korean Journal of Microbiology
    • /
    • v.24 no.1
    • /
    • pp.7-11
    • /
    • 1986
  • We screened lysates of the laboratory strains of pseudomonads utilizing hydrocarbon by agarose gel electrophoresis and cesium chloride-ethidium bromide equilibrium centrifugation, to find an intrinsic plasmid as a vector and to examine the relationship between the plasmid and hydrocarbon degradation. Only one strain from the examined strains, Pseudomonas putida KU190, contained a plasmid. We named the plasmid pKU41. The molecular size of pKU41 was determined as 41kb, using covalently closed circular forms of RP4 and pSY343 as standard size markers. The restriction sites of pKU41 for BamHI, BglII, EcoRI, HindIII, and SalI were 3, 1, 3, 6 and more than 13, respectively. With double or triple digestion, restriction map of pKU41 was constructed for BamHI, BglII and HindIII. For elucidation on the biological function of the plasmid, test was conducted on the ability of hydrocarbon utilization of the host strain but no apparent relationship was observed.

  • PDF

Cryogenic fracture behaviors and polarization characteristics according to sensitizing heat treatment on structural material of the nuclear fusion reactor (핵 융합로 구조재료의 예민화 열처리에 따른 극저온 파괴거동 및 분극특성)

  • Kwon, Il-Hyun;Chung, Se-Hi
    • Transactions of the Korean Society of Mechanical Engineers A
    • /
    • v.22 no.2
    • /
    • pp.311-320
    • /
    • 1998
  • The cryogenic fracture behaviors of austenitic stainless steel HN2 developed for nuclear fusion reactor were evaluated quantitatively by using the small punch(SP) test. The electrochemical polarization test was applied to study thermal aging degradation of HN2 steel. The X-ray diffraction(XRD) analysis was conducted to detect carbides and nitrides precipitated on the grain boundary of the heat treated HN2 steel. The mechanical properties of the HN2 steel significantly decreased with increasing time and temperature of heat treatment or with decreasing testing temperature. The integrated charge(Q) obtained from electrochemical polarization test showed a good correlation with the SP energy(ESP) obtained by means of SP tests. From the results observed in the x-ray diffraction and anodic polarization curve, it was known that the material the grain boundary. Combining SP test and electrochemical polarization test, it could be useful tools to non-destructively evaluate the cryogenic fracture behaviors and the aging degradation for cryogenic structural material.

A Study on the load control using electric inertia

  • Kim, Gil-Dong;Park, Hyun-Jun;Han, Young-Jae;Jang, Dong-Yuk;Jo, Jung-Min
    • 제어로봇시스템학회:학술대회논문집
    • /
    • 2001.10a
    • /
    • pp.128.1-128
    • /
    • 2001
  • A propulsion system apparatus is needed for a railroad vehicle to test and estimate propulsion performance. The electrical inertia simulator to facilitate the development and testing of propulsion systems, is presented in this paper. It is based on a vector-controlled Induction motor drive supplied from the AC mains through a double PWM converter that provides desirable features such as hi-directional power folw, nearly unity power factor and low harmonic factor at the Ac mains. A theoretical analysis is first presented, followed by a detailed simulation study to assess the overall system performance under dynamic conditions.

  • PDF

Anthelmintic Efficacy Test of Levamisole HCl and Oxyclozanide against the Flukes and Gastrointestinal Roundworms of Cattle (소(우)의 흡충류 및 소화관내선충류에 대한 Levamisol HCl 및 Oxyclozanide 합제의 구충효능시험)

  • Jang Du-Hwan;Jee Cha-Ho;Youn Hi-Joeng
    • Journal of the korean veterinary medical association
    • /
    • v.20 no.5
    • /
    • pp.309-313
    • /
    • 1984
  • A total of 290 cattle in several districts was selected for treatment against the liver flukes, paramphistomes, and the gastrointestinal roundworms from March to september, 1983. 1. Egg reduction rates against the liver flukes, paramphistomes, and gastroi

  • PDF

Studies on the Influence of Various Factors on 2, 3, 5-Triphenyl Tetrazolium Chloride Reduction Test(T.T.C. Test) (2, 3, 5-Triphenyl Tetrazolium Chloride 환원시험(還元試驗)(T. T. C. Test)에 미치는 요인(要因)에 관한 연구(硏究))

  • Kim, Kyo-Joon;Kim, Sang Keun
    • Korean Journal of Agricultural Science
    • /
    • v.5 no.2
    • /
    • pp.80-86
    • /
    • 1978
  • This study was carried out in order to know the effect of T. T. C. reaction on antibiotics, cleanser, disinfectants, pH, acidity and station of culture a bacterial Streptococcus thermophilus was used to 2.3.5-triphenyl tetrazolium chloride reduction test for the detection of residual antibiotics in milk were tested. The results obtained were summarized as follows. 1. The T. T. C. reaction was most sensitive in the group of penicillin as 0.02~0.05 IU and also it was less sensitive in Streptomycin and various broad antibiotics except Vibramycin, Synthomycin and Teramycin but it was least sensitive in Streptomycin group. 2. For ther T. T. C. reaction test on cleanser, the positive reaction was appeared above 0.7% of Hi-Ti and 0.1% of NaOH solution respectively. 3. For the T. T. C. test reaction on disinfectants, the positive reaction was occured on 200~500 ppm solution of sodium-hypochlorite and chloride-lime, 0.15~0.35% solution of phenol, cresol and Iode-K. 4. The optimum temperature was $35{\sim}37^{\circ}C$ and proper culturing time was 12 to 18 hours for propagation culturing. 5. The suspect positive reaction was tested above pH 5.0 and the positive reaction was appeared above 0.21% of acidity.

  • PDF

Character Analysis of Micro Fuse Fusing as a function of De-Rating technique (디레이팅 기법에 의한 마이크로 퓨즈 용단의 특성 분석)

  • Kim, Do-Kyeong;Kim, Jong-Sick
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
    • /
    • v.29 no.6
    • /
    • pp.8-13
    • /
    • 2015
  • Recently, Illumination industry of LED module has been focused to industry technology for energy conservation of nation. The LED device is excellent to power efficiency due to semiconductor light source element. And the application to the lighting circuit technology can be designed to the sensitive lighting system for human sensitivity control. In this paper, as a process for analyzing the operating temperature of standardized electronic device including LED device has analyzed about fusing character with in designed micro fuse for electronic device protection from the over current. Using the de-rating technique, which is performed to micro fuse fusing test in the range of $-30^{\circ}C{\sim}120^{\circ}C$ thermostatic chamber. To the output data in each temperature zone, it is performed to first-order linear fitting. Additionally, applying the resistance temperature coefficient and statistical data for the reliable analysis has derived to the metal element resistance of micro fuse with temperature change of the thermostatic chamber. As a research result, The changed temperature effect of thermostatic chamber was confirmed regarding fusing time change.

Detection of Porno Sites on the Web using Fuzzy Inference (퍼지추론을 적용한 웹 음란문서 검출)

  • 김병만;최상필;노순억;김종완
    • Journal of the Korean Institute of Intelligent Systems
    • /
    • v.11 no.5
    • /
    • pp.419-425
    • /
    • 2001
  • A method to detect lots of porno documents on the internet is presented in this parer. The proposed method applies fuzzy inference mechanism to the conventional information retrieval techniques. First, several example sites on porno arc provided by users and then candidate words representing for porno documents are extracted from theme documents. In this process, lexical analysis and stemming are performed. Then, several values such as tole term frequency(TF), the document frequency(DF), and the Heuristic Information(HI) Is computed for each candidate word. Finally, fuzzy inference is performed with the above three values to weight candidate words. The weights of candidate words arc used to determine whether a liven site is sexual or not. From experiments on small test collection, the proposed method was shown useful to detect the sexual sites automatically.

  • PDF

A Study on the Characteristics of Automatic Flatness Control System for Stell Sheet (강판의 자동 형상제어 장치의 특성에 관한 연구)

  • 김순경;전연찬;김중완;김문경
    • Proceedings of the Korean Society of Precision Engineering Conference
    • /
    • 1996.04a
    • /
    • pp.541-545
    • /
    • 1996
  • In this paper, The performance and functions of automatic flatness control system installed on the 4 hi-reversing mill and has been investigated under actualconditions. A new automatic flatness control system incorporates a measuring roll for measurement and correction calculations, hydraulic roll benders, selective roll cooling, and a programmable controller for interface and data logging. The test results are as following. The more the exit steel strip thickness is thick, the smaller the I value, and the more it is thin, the larger the I value. And, a complex distribution of strip tension was controlled, for example, not only a quarter buckle but also a simple center wave and edge wave. Because the tension deviation is larger at acceleration speed and deceleration speed than steady speed, so automatic flatness control system of contact type is better to adopt over 450 m/min, automatic flatness control system reduces rapidly large flatness deviation. The maximum I value of strip has been decreased to 13 I, and defects caused by poor flatness have been drastically decreased. And coolant temperature for work roll cooling system on the automatic flatness control system is better to adopt about 50-55 .deg. C.

  • PDF

The Effect of the Heat Treatment Conditions on the Strength and Microstructure in the Bonded Interface in Dissimilar Metal and Aluminum Alloy (AL합금과 이종금속의 접합계면에서의 미세조직과 접합강도에 미치는 열처리조건의 영향)

  • Kim, Ick-Soo;Choi, Byung-Young;Kang, Chang-Yong
    • Journal of the Korean Society for Heat Treatment
    • /
    • v.16 no.1
    • /
    • pp.2-9
    • /
    • 2003
  • The aluminum alloy which is light and has excellent thermal conductivity and iron base alloy that is remarkable heat-resistece and wear resistence properties were bonded together. The bond was created between a stationary and a rotating member by using the frictional heat generated between them while subjected to high normal forces on the interface of Al alloy and iron base alloy. The microstructure of the bonded interface of friction welding and the strength in the bonded interface formed under various bonding conditions were examined through TEM, SEM with EDX and triple bending test. In interface of bonding materials formed after various heat treatment, bonding strength was substantially different, resulting from formation of intermetallic compound or softening during annealing.