• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.2
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• pp.304-310
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• 2006

Prevotella intermedia is one of the most frequently implicated pathogens in human periodontal disease and has a requirement for hemin for growth. This study has identified a hemin-binding P. intermedia protein by expression of a P. intermedia genomic library in Escherichia coli, a bacterium which does not require or transport exogenous hemin. The genomic library of P. intermedia was constructed into plasmid pUC18, transformed into Escherichia coli strain $DH5{\alpha}$, and screened for recombinant clones using heminbinding activity by plating onto hemin-containing agar. Approximately 5,000 recombinant E. coli colonies were screened onto LB-amp-hemin agar, single clone(pHem1) was exhibited a clearly pigmented phonotype. The 2.5kb insert DNA of pHem1 was determined by restriction enzyme mapping. Southern blot analysis of BamHI, BglII, EcoRI, HindIII and PstI-digested P. intermedia DNA indicated that single copy of the gene was present in the genome. Northern blot analysis revealed that the size of transcript was approximately 1.8 kb. The cloned gene contained a single ORF, consisting of approximately 850-residue amino acids. A BLAST search of the Institute for Genomic Research genes with similar nucleotide sequence revealed no significant similarity It needs further investigation to clarify the mechanisms of heme uptake in P. intermedia.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2001.05a
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• pp.23-28
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• 2001

Since non-corrosive Fiber Reinforced Polymer(FRP) tendons have been in increasing use for underground and coastal structures constantly contacted with fresh water or sea water because of their superiority to metallic ones in corrosion-resistance, new non-metallic anchoring system for FRP tendons has been developed and investigated to verify the effectiveness of tendon force, which consist of mainly FRP pipes and Highly Expansive Mortar(HEM). The major factors considered in this experiment were expansive pressures of HEM during its hydration, sleeve lengths and types, and anchoring methods of tendon. New anchoring system were investigated from the pull-out tests. The pull-out procedures of the FRP tendons in the various pipe filled with HEM were analyzed and improved ideas were suggested to develop novel non-metallic anchoring system for FRP tendons The pull-out tests for the FRP tendon and new non-metallic anchoring system were conducted. The results show that non-metallic anchoring system for the FRP tendon has been more stablized due to the gradual expansive pressrure of HEM, as tims goes. Since tile lower stiffness of FRP pipes causes the weakness of anchoring force, it requires the increase of stiffness using a carbon fiber or an increased section area.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1327-1332
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• 2004

The hemA gene encoding 5-aminolevulinic acid synthase (ALAS) was cloned from Rhodobacter capsulatus, and its nucleotide sequence was determined. DNA sequencing data revealed one open reading frame coding for a protein with 401 amino acids that displayed high similarity to the amino acid sequences of other known ALASs. The hemA gene was then cloned and expressed under the control of constitutive promotor in E. coli. The recombinant E. coli strain was able to accumulate 5-aminolevulinic acid to 21 mM in the liquid medium supplemented with 45 mM glycine and 120 mM succinate. In addition, a marked effect of the pH of the culture medium on ALA production was observed, and the optimum pH for culture medium was determined to be 5.8-6.3.

• Journal of the Korea Fashion and Costume Design Association
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• v.7 no.1
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• pp.33-40
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• 2005

Gathering is method used to control fullness along a seam line. The purpose of this study was to investigate the relationship between the quantitative research and qualitative method; the effect of gather and the types of gather drape. The experimental design consists of four factors: (l) three kinds of different weight and thickness of fabrics (2) three kinds of stitch densities (3) five kinds of ratio of gathers (4) three kinds of grain directions. Therefore one hundred thirty five (135) samples were made. And utilized SPSS WIN 10.0 Package in data analysis. The results of this study were as follows; First, after frequency analysis, side height, hem line width, node depth, node count, node width accorded with these result data recording. Second, after correlation analysis, side height related with front statements. Side height and entire visual was negative correlation. Hem line width, node depth, node count with section statements was negative correlation but node width at section statements was positive correlation. Third, after $k^2$ analysis, front picture parts getting excellent evaluation were 1st side height, 3rd hem line width, 4th node depth, 3rd node count, 3rd node width. And section illustration parts getting excellent evaluation were 4th side height, 1st hem line width, 2nd node depth, 3rd node count, 4th node width.

• Journal of Microbiology and Biotechnology
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• v.17 no.9
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• pp.1579-1584
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• 2007

5-Aminolevulinate (ALA) synthase (E.C. 2.3.1.37), which mediates the pyridoxal phosphate-dependent condensation of glycine and succinyl-CoA, encoded by the Rhodobacter sphaeroides hemA gene, enables Escherichia coli strains to produce ALA at a low level. To study the effect of the enhanced C4 metabolism of E. coli on ALA biosynthesis, NADP-dependent malic enzyme (maeB, E.C. 1.1.1.40) was coexpressed with ALA synthase in E. coli. The concentration of ALA was two times greater in cells coexpressing maeB and hemA than in cells expressing hemA alone under anaerobic conditions with medium containing glucose and glycine. Enhanced ALA synthase activity via coupled expression of hemA and maeB may lead to metabolic engineering of E. coli capable of large-scale ALA production.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.101-105
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• 2005

Hemming process, composed of flanging, pre-hemming and main hemming, is the last one of a series of forming processes conducted on the automotive panels, having a great influence on the outward appearance of them. The hem quality can be quantitatively defined by the hemming defects including turn-down, warp and roll-in. However, it is difficult to evaluate and predict the hem quality through the experimental measurement or the numerical calculation since the size of defects is very small. This study is focused on how to simulate in the finite element analysis (FEA) the same conditions as in the experiment. The FEA result on turn-down, that was obtained from a finite element model including the spring element linked to the flanging pad, had a good correlation with the experimental data. It was found that the radius of curvature of the flange deeply affects the final hem quality and therefore high rigidity of forming tools and tight assembling tolerance are highly recommended. An over-stroke of the main hemming punch is also proposed to reduce the turn-down.

• Journal of the Korea Fashion and Costume Design Association
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• v.16 no.1
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• pp.15-25
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• 2014

The purpose of this study is to recognize the differences of visual evaluation by variations in width of hem line and waistline position of the bell buttom pants. The stimuli are 9 samples: One control group, 3 variations of the width of hem line and 3 variations of the waistline position. The data has been obtained from 56 fashion students. The data has been analyzed by Factor Analysis, Anova, Scheffe's Test and the MCA method. The results of the study are as follows: The visual evaluation by the width of hem line and waistline position of bell buttom pants are composed of 5 factors : comfort, personality, physical characteristics, stiffness and modern. Among these factors, the comfort is evaluated to be the most important factor. As a visual evaluation result of changes in the width of hern line, 62cm in width (the narrowest width) was highly evaluated in comfort and stiffness factors, 78cm in width (the widest width) was highly evaluated in personality and physical characteristics factors. For the result of changes in the waistline position, high-waisted bell buttom pants were was highly evaluated in personality and stiffness factors, low-waisted bell buttom pants were was highly evaluated in comfort, physical characteristics and modern factors. The width of hem line and waistline position of the bell buttom pants interacted to the comfort factor. The width of hem line had more influence on visual evaluation in personality factors while physical characteristics, stiffness and modern factors were affected by the waistline position.

• Journal of the Korean Society of Costume
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• v.33
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• pp.217-228
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• 1997

The purpose of this study was to investigate influence of the construction method of the circular skirt on drapability and shape of static silhouette and length variation. In made 24 types of skirts giving the variations (six kinds of peach skin-like finished fabrics two types of cutting method two types of machine stitch method of hem), The results were as follows: 1. Analysis of drapability In the cutting method thre was similarity between warp direction and true bias direc-tion. In the machine stitch method of hem there was similarity between blind stitch and blind stitch machine. According to the fabrics analysis of drapability was excellent in the order N/P 80/20(fabric 1)>P 100(fabric 5)>P 100(fabric 6)>P 100 (fabric 2)>P 100 (fabric 4)>N/C 50/50(fabric 3). 2. Analysis of the characteristics values of static silhouette shape In the cutting method shape of static sil-houette became wide in the warp direction. In the machine stitch method of hem shape of static silhouette became wide in the blind stitch. According to the fabrics shape of static silhouette became most wide in the N/C 50/50(fabric 3) and shape of static sil-houette become most narrow in the P 100(fabric 2,5) 3. Analysis of the length variation In the cutting method the true bias direc-tion was longer than the warp direction. In the machine stitch method of hem there was simi-machine stitch method of hem there was simi-larity between blind stitch and blind stitch ma-chine. In fabrics the P 100(fabric 6) showed the longest the N/C 50/50(fabric 3) showed most slight. Interaction between the cutting and messure-ment part of skirt lengh. In the warp diretion parts that showed longest length variation were C. G, K, O in the true bias diretion parts that showed most slight length variation were A, E, M, I.

• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.157-166
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• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• Journal of the Korean Home Economics Association
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• v.33 no.4
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• pp.149-155
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• 1995

In this study, the formative property of flare skirts is carry out by the shape horizontal sections of hem line. Flare skirts was made by 10 kinds of fabrics with different physical properties. the length of flare skirts was 60cm. The main results obtained are as follows ; 1, The shape horizontal sections of hem line has differed with number of nodes and fabrics properties, in direction of texture. 2. According to the fabrics analysis of drapability decreased in order from Polyester/nylon(20/80)>Polyester(twill, thickness 0.2441)>Polyester(plain, thickness0.3760)>Polyester(plain, thickness 0.3687)>Polyester(plain, thickness 0.3101). 3. The correlation between the number of nodes and wave-height of modes and breadth showed a high negative correlation with the drapability of fabrics.