• Molecules and Cells
• /
• v.37 no.9
• /
• pp.637-643
• /
• 2014

Wound healing is a complex multi-step process that requires spatial and temporal orchestration of cellular and non-cellular components. Hypoxia is one of the prominent microenvironmental factors in tissue injury and wound healing. Hypoxic responses, mainly mediated by a master transcription factor of oxygen homeostasis, hypoxia-inducible factor-1 (HIF-1), have been shown to be critically involved in virtually all processes of wound healing and remodeling. Yet, mechanisms underlying hypoxic regulation of wound healing are still poorly understood. Better understanding of how the wound healing process is regulated by the hypoxic microenvironment and HIF-1 signaling pathway will provide insight into the development of a novel therapeutic strategy for impaired wound healing conditions such as diabetic wound and fibrosis. In this review, we will discuss recent studies illuminating the roles of HIF-1 in physiologic and pathologic wound repair and further, the therapeutic potentials of HIF-1 stabilization or inhibition.

• Journal of People, Plants, and Environment
• /
• v.23 no.1
• /
• pp.77-85
• /
• 2020

The purpose of this study was to examine the effects of a healing garden program based on internal factors of vocational adaptation on career attitude for college students with developmental disabilities. First, healing garden program was defined by a group of experts related to education for developmental disabilities in the industry and academia through the focus group interview. Second, the healing garden program for college students with developmental disabilities was carried out for 15 weeks. Third, this study examined the effects of healing garden program based on internal factors of vocational adaptation on career attitude for college students with developmental disabilities. The participants of this study were 55 college students with developmental disabilities in N University, with 25 students placed in the experimental group and the other 30 in the control group. As a result of examination, the difference between before and after the program, it was found that factors of career attitude were improved significantly after the program in the experimental group, with Factor 1 (determination) increased from 2.08 to 3.39, Factor 2 (finality) from 2.28 to 3.19, Factor 3 (confidence) from 2.20 to 3.69, Factor 4 (preparation) from 2.12 to 3.79, and Factor 5 (independence) from 1.88 to 3.63. But the control group did not show a significant improvement after 15 weeks of the program except Factor 3 (confidence) and Factor 5 (independence). It was found that the healing garden program based on internal factors of vocational adaptation had effects on career attitude for college students with developmental disabilities. Finally, the program will contribute to vocational rehabilitation for college students with disabilities. The findings further suggest that healing gardening programs that facilitate career attitudes must be continuously developed and applied in order to establish effective transition from school to the world of vocational adaptation.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.22 no.2
• /
• pp.15-23
• /
• 2016

Purpose: Planning space of the house is important as a universal place to relieve stress of contemporary people. Void space is easy to perform mental and cultural programs in aspect of affording the healing behavior. The "Interface" accommodates healing behaviors through mutual interchange between void space and solid space as the edge of the solid space around void space. This study presents a efficient theory of healing space through combining space theory with the Kitwood's theory of psychiatry used for the art therapy. Methods: Spatial elements based on Kitwood's theory converges towards 2 behavioral elements; Meditation(-), Sense of Community(+). Balancing with reciprocal complement between behaviors of passive and stable "Meditation(-)" and active and released "Sense of Community(+)" was analysed. Results: "Faciliation" can be used in combination with "Flow(+)" as active factor. "Validation" is combined with "Prospect(+,-)", and divides into 2 factor ; "Recognition-Refreshment(-)", "Reaction-Social Exchange(+)". "Holding" is combined with "Refuge(-)" as passive factor. Implications: This study established concept of spatial healing elements in connection with theory of Psychiatry about Alzheimer that is extreme situation of stress. This study is expected to enhance the validity and efficiency in analysis of healing space.

• Archives of Plastic Surgery
• /
• v.33 no.1
• /
• pp.1-4
• /
• 2006

Cryopreserved fibroblast implants represent a major advancement for healing of chronic wounds. Bone marrow stromal cells, which include the mesenchymal stem cells, have a low immunity-assisted rejection and are capable of expanding profoundly in a culture media. Therefore, they have several advantages over fibroblasts in clinical use. The ultimate goal of this study was to compare the wound healing accelerating growth factor secretion of the bone marrow stromal cells with that of the fibroblasts and this pilot study particularly focuses on the growth factor secretion to accelerate wound healing. Bone marrow stromal cells and fibroblasts were isolated from the same patients and grown in culture. At 1, 3, and 5 days post-incubating, secretion of basic fibroblast growth factor(bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta(TGF-${\beta}$) were compared. In TGF-${\beta}$ secretion fibroblasts showed 12~21% superior results than bone marrow stromal cells. In contrast, bFGF levels in the bone marrow stromal cells were 47~89% greater than that in fibroblasts. The VEGF levels of the bone marrow stromal cells was 7~12 fold greater than that of the fibroblasts. Our results suggest that the bone marrow stromal cells have great potential for wound healing accelerating growth factor secretion.

• Journal of Korean Medicine Rehabilitation
• /
• v.30 no.2
• /
• pp.19-35
• /
• 2020

Objectives The aim of this study is to evaluate the fracture healing effect of Jinmu-tang (JM) on femur fractured rats. Methods Rats were randomly divided into 5 groups (normal, control, positive control, JM extract with low concentration and JM extract with high concentration). All group except normal group went through both femur fracture. Normal and control group received no treatment at all. Positive control group were medicated with tramadol (20 mg/kg) once a day for 14 days. Experimental group was orally medicated with JM extract (10 mg/kg for low concentration, 50 mg/kg for high concentration) once a day for 14 days. In order to investigate fracture healing process, plasma and serum were obtained. Also, micro-computed tomography was conducted to see the frature site visually. Immunohistochemistry for transforming growth factor-β1, Ki67, alkaline phosphatase, runt-related transcription factor 2, receptor activator of nuclear factor kappa-β, tartrate resistant acid phosphatase was conducted to observe bone healing progress after 14 days since fracture occured. Aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels were measured in plasma, for hepatotoxicity and nephrotoxicity of JM extract. Osteocalcin was measured to observe activity of osteoblast. Results Through Micro-CT, more fracture healing was observed on both experimental group than control and positive control group. Through Hematoxylin & Eosin and safranin O staining showed bone cell proliferation and bone formation in the experimental group. RANK was significantly increased in the experimental groups. JM with high concentration showed statistically significant of TGF-β and Osteocalcin. NO, TRAP and ALP were not significantly changed. Liver toxicity was not significantly observed. Creatinine significantly increased in both experimental groups after 28 days. Conclusions As described above, JM extract showed anti-inflammatory effect, promoted fracture healing by stimulating the bone regeneration factor, and showed little hepatotoxicity and nephrotoxicity. In conclusion, JM extract can promote fracture healing and it can be used clinically to patients with fracture.

• Journal of Environmental Science International
• /
• v.25 no.7
• /
• pp.963-972
• /
• 2016

A survey was performed to present a scheme for building a healing forest park inside a city in consideration of the fact that healing forests are located in non-urban areas because of their nature, and are therefore not conveniently accessible to urban population (n=196). As a result, among 196 respondents to the survey, 74.5% indicated that it was necessary to build an urban healing forest park. In the case of the expected usage pattern, were an urban forest park to be built, the largest proportion responded that it would be used to "walk", followed by "rest and meditation" and then "meeting/talk/date". An analysis showed that the largest proportion responded that they factor in "Accessibility" (48%), followed by "Park condition" (16.8%), and "Vegetation type" (12.2%). According to the analysis of usage preference for healing therapy, it was shown that the largest proportion preferred "Terrain therapy" (37.8%), followed by "Exercise therapy" (19.9%), and "Naturopathy" (18.9%). The factor anlaysis of usage preference regarding urban healing forest park facilities showed that the components were divided into "Operation facilities" and "Natural healing space", and the largest proportion preferred "Trails for healing", followed by "Space or forest for meditation" and "Health promotion center", and then "Facilities for a exercise therapy". In building an urban healing forest park, we think that it is effective to introduce zones and facilities in consideration of park characteristics that are centered on everyday behaviors, such as taking a walk, which differ from those of healing forests located in non-urban areas.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.10
• /
• pp.1661-1669
• /
• 2007

Gamma-aminobutyric acid (GABA) is a non-protein amino acid. It is well known for its role as an inhibitory neurotransmitter of developing and operating nervous systems in brains. In this study, a novel function of GABA in the healing process of cutaneous wounds was presented regarding anti-inflammation and fibroblast cell proliferation. The cell proliferation activity of GABA was verified through an MTT assay using murine fibroblast NIH3T3 cells. It was observed that GABA significantly inhibited the mRNA expression of iNOS, IL-$1{\beta}$, and TNF-${\alpha}$ in LPS-stimulated RAW 264.7 cells. To evaluate in vivo activity of GABA in wound healing, excisional open wounds were made on the dorsal sides of Sprague-Dawley rats under anesthesia, and the healing of the wounds was apparently assessed. The molecular aspects of the healing process were also investigated by hematoxylineosin staining of the healed skin, displaying the degrees of re-epithelialization and linear alignment of the granulation tissue, and immunostaining and RT-PCR analyses of fibroblast growth factor and platelet-derived growth factor, implying extracellular matrix synthesis and remodeling of the skin. The GABA treatment was effective to accelerate the healing process by suppressing inflammation and stimulating re-epithelialization, compared with the epidermal growth factor treatment. The healing effect of GABA was remarkable at the early stage of wound healing, which resulted in significant reduction of the whole healing period.

• Biomolecules & Therapeutics
• /
• v.7 no.1
• /
• pp.7-13
• /
• 1999

The stimulatory effect of recombinant human basic fibroblast growth factor (bFGF) on wound healing was evaluated in healing-impaired animal models. Full-thickness wounds were made in prednisolone-treated mice, streptozotocin (STZ)-induced diabetic rats and mitomycin C (MMC)-treated rats. Saline or bFGF at a dose of 1, 5, or $25\mu\textrm{g}$ per wound was applied to the open wound once a day for three to five days. The degree of wound healing was assessed using wound size and histological parameters such as degree of epidermal and dermal regeneration. Local application of bFGF accelerated wound closure significantly in a dose-dependent manner in all healing-impaired wounds (p<0.05). The wound healing effect of bFGF was further confirmed by histological examination in MMC-treated rats. Epidermal and dermal regeneration were enhanced in bFGF-treated wounds with a dose-related response. Dermal regeneration parameters such as collagen matrix formation and angiogenesis were significantly increased in $5\mu\textrm{g}$, or $\25mu\textrm{g}$ of bFGF-treated wounds when compared to saline-treated wounds (p<0.05). pectin immunostaining on day 8 for vascular endothelium showed an increased number of neovessels in bFGF-treated wounds. These results suggest that topical application of bFGF has beneficial effects on wound healing by angiogenesis and granulation tissue formation in healing-impaired wounds.

• Journal of Industrial and Engineering Chemistry
• /
• v.67
• /
• pp.365-372
• /
• 2018

Wounds that heal with excessive scar formation result in poor functional and aesthetic outcomes. To address this, in our study, visible light cured glycol chitosan (GCH) hydrogels containing endothelial growth factor (EGF) and basic fibroblast growth factor (bFGF) were prepared (GCH-EGF, GCH-FGF and GCH-EGF/FGF) and evaluated their efficacies on the improvement of wound healing in vivo. In vitro release test showed that the growth factors were released in a sustained manner along with initial burst for 24 h. In vitro cell proliferation assay of L-929 mouse fibroblast cell line resulted in the superior ability of GCH-EGF/FGF on the rate. In vivo results demonstrated that the growth factor loaded GCHs further enhanced wound healing compared with GCH. In particular, GCH-EGF/EFG showed the most remarkable wound healing effect among the samples.

• Current Optics and Photonics
• /
• v.3 no.6
• /
• pp.485-495
• /
• 2019

Photobiomodulation (PBM) using organic light emitting diodes (OLEDs) surface light sources have recently been claimed to be the next generation of PBM light sources. However, the differences between light emitting diodes (LEDs) and OLED mechanisms in vitro and in vivo have not been well studied. In vivo mouse models were used to investigate the effects of OLED irradiation on cellular function and cutaneous wound healing compared to LED irradiation. Mice in the LED- and OLED-irradiated groups were subjected to irradiation with 6 J/㎠ LED and OLED (630 nm), respectively, for 14 days after wounding, and some mice were sacrificed for the experiments on days 3, 7, 10, and 14. To evaluate wound healing, we performed hematoxylin-eosin and Masson's trichrome staining and quantified collagen density by computerized image analysis. The results showed that the size of the wound, collagen density, neo-epidermis thickness, number of new blood vessels, and number of fibroblasts and neutrophils was significantly influenced by LED and OLED irradiation. The tissue levels of interleukin (IL)-β, IL-6 and tumor necrosis factor (TNF)-α were investigated by immunohistochemical staining. LED and OLED irradiation resulted in a significant increase in the tissue IL-β and IL-6 levels at the early stage of wound healing (P < 0.01), and a decrease in the tissue TNF-α level at all stages of wound healing (P < 0.05), compared to the no-treatment group. The expression levels of the genes encoding vascular endothelial growth factor and transforming growth factor-beta 1 were significantly increased in LED and OLED-irradiated wound tissue at the early stage of wound healing (P < 0.01) compared to the no-treatment group. Thus, OLED as well as LED irradiation accelerated wound healing by modulating the synthesis of anti-inflammatory cytokines and the expression levels of genes encoding growth factors, promoting collagen regeneration and reducing scarring. In conclusion, this suggests the possibility of OLED as a new light source to overcome the limitations of existing PBMs.