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Genetic diversity of Acanthamoeba isolates from ocean sediments

  • Liu, Hua;Ha, Young-Ran;Lee, Sung-Tae;Hong, Yean-Chul;Kong, Hyun-Hee;Chung, Dong-Il
    • Parasites, Hosts and Diseases
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    • v.44 no.2 s.138
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    • pp.117-125
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    • 2006
  • Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial cells versus reference strains. All isolates belonged to morphologic group II. Total of 16 restriction phenotypes of mtDNA from 18 isolates demonstrated the genetic diversity of Acanthamoeba in ocean sediments. Phylogenetic analysis using 18s rDNA sequences revealed that the 18 isolates were distinct from morphological groups I and III. Fifteen isolates showed close relatedness with 17 clinical isolates and A. castellanii Castellani and formed a lineage equivalent to T4 genotype of Byers' group. Two reference strains from ocean sediment, A. hatchetti BH-2 and A. griffini S-7 clustered unequivocally with these 15 isolates. Diversity among isolates was also evident from their cytopathic effects on human corneal cells. This is the first time describing Acanthamoeba diversity in ocean sediments in Korea.

Evaluation of Evacuation Safety of High School According to Change in the Width of Hallway

  • Seon-Yong Jeong;Hong-Sang Lee;Ha-Sung Kong
    • International Journal of Internet, Broadcasting and Communication
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    • v.15 no.2
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    • pp.60-72
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    • 2023
  • The purpose of this study is to measure the change in evacuation time at high schools according to the change in hallway width using an evacuation simulation program and to analyze the result of such change. In order to measure the evacuation time according to the change in the hallway width according to the 「Rules on the Standards for Evacuation and Fire Protection Structures of Buildings」 and to analyze the change in evacuation time resulted from the increase in the number of occupants, a scenario was constructed by applying the 「performance-oriented design method and standard for firefighting facilities, etc.」. As a result of the experiment, it was found that the evacuation time was the shortest when the width of the hallway was the widest, which was 3m. On the other hand, the evacuation time took the longest at 1.8m, which was the width of the second narrowest hallway. For the safety of high school students who spend a lot of time at school, it is necessary to secure a wide hallway width when building a new school or to provide periodic safety education in the case of an existing school whose hallways are considered narrow.

Isolation of N-Acetylmuramoyl-L-Alanine Amidase Gene (amiB) from Vibrio anguillarum and the Effect of amiB Gene Deletion on Stress Responses

  • Ahn Sun-Hee;Kim Dong-Gyun;Jeong Seung-Ha;Hong Gyeong-Eun;Kong In-Soo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.9
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    • pp.1416-1421
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    • 2006
  • We identified a gene encoding the N-acetylmuramoyl L-alanine amidase (amiB) of Vibrio anguillarum, which catalyzes the degradation of peptidoglycan in bacteria. The entire open reading frame (ORF) of the amiB gene was composed of 1,722 nucleotides and 573 amino acids. The deduced amino acid sequence of AmiB showed a modular structure with two main domains; an N-terminal region exhibiting an Ami domain and three highly conserved, continuously repeating LysM domains in the C-terminal portion. An amiB mutant was constructed by homologous recombination to study the biochemical function of the AmiB protein in V. anguillarum. Transmission electron microscopy (TEM) revealed morphological differences, and that the mutant strain formed trimeric and tetrameric unseparated cells, suggesting that this enzyme is involved in the separation of daughter cells after cell division. Furthermore, inactivation of the amiB gene resulted in a marked increase of sensitivity to oxidative stress and organic acids.

Thermal stability and Young's modulus of mechanically exfoliated flexible mica

  • Jin, Da Woon;Ko, Young Joon;Kong, Dae Sol;Kim, Hyun Ki;Ha, Jae-Hyun;Lee, Minbaek;Hong, Jung-Il;Jung, Jong Hoon
    • Current Applied Physics
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    • v.18 no.12
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    • pp.1486-1491
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    • 2018
  • In recent years, mica has been successfully used as a substrate for the growth of flexible epitaxial ferroelectric oxide thin films. Here, we systematically investigated the flexibility of mica in terms of its thickness, repeated bending/unbending, extremely hot/cold conditions, and successive thermal cycling. A $20-{\mu}m-thick$ sheet of mica is flexible even up to the bending radius of 5 mm, and it is durable for 20,000 cycles of up- and down-bending. In addition, the mica shows flexibility at 10 and 773 K, and thermal cycling stability for the temperature variation of ca. 400 K. Compared with the widely used flexible polyimide, mica has a significantly higher Young's modulus (ca. 5.4 GPa) and negligible hysteresis in the force-displacement curve. These results show that mica should be a suitable substrate for piezoelectric energy-harvesting applications of ferroelectric oxide thin films at extremely low and high temperatures.

Analysis of evacuation time for New publicly used establishments according to whether safety facilities, etc. are installed

  • Hong-Sang Lee;Ha-Sung, Kong
    • International Journal of Internet, Broadcasting and Communication
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    • v.15 no.2
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    • pp.49-59
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    • 2023
  • In accordance with the revision of "Special act on the safety control of publicly used establishments", this study is aim to measure the change in evacuation time due to whether safety facilities, etc. are installed of a room escape cafe business and kids cafe business(hereinafter referred to as "New publicly used establishments"), which were added as new targets of the publicly used business from June 8, 2022. In the case of new publicly used establishments or publicly used establishments whose owners are changed after the revision of the relevant laws, safety facilities, etc. are installed and maintained under the "Special act on the safety control of publicly used establishments", but in the case of existing businesses that have been operating even before the revision of the law, the business continues without safety facilities, etc. installed because the revised law is not retroactively applied. The purpose of this study is to compare and analyze the change in evacuation time by measuring the evacuation time to operating before the revision of the law to simulate evacuation at existing new publicly used establishments without safety facilities, etc. and measure the evacuation time at new publicly used establishments with safety facilities after the revision of the law

Analysis of the Number of Kinks with the Fire Hose Staking Method of Indoor Hydrant System (옥내소화전설비의 소방호스 적재방식에 따른 꼬임횟수 분석)

  • Hong, Suk-Hwan;Kong, Ha-Sung
    • Journal of the Korea Safety Management & Science
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    • v.23 no.4
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    • pp.61-66
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    • 2021
  • In this study, according to the staking method of the fire hose in the indoor hydrant system, the number of kinks when the fire hose is deployed was measured and analyzed to examine how the staking method of the fire hose affects the kink when the hose is deployed. The fire hose staking method was classified into three types: donut-type, accordion-type, and hose hanger-type, and the walking speed and number of kinks were measured by repeating the experiment 5 times for male and female students. The results of the study are as follows. (1) Among the three types of fire hose staking methods, the donut-type staking method had an average of 92 times for male students and 86 times for female students on average. (2) The accordion-type and hose hanger-type staking methods showed similar results with an average of one or two kinks for both male and female students. (3) Regardless of the staking method, it was found that in general, male students with high walking speed had more kinking than female students with slow walking speed.

A Study on the Current Status of Hanbok Education in the Era of Fashion Hanbok (패션한복시대의 한복 전문교육 현황 연구)

  • Yun, So Jung;Jang, Ju Yeun;Lee, Ha Kyung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.46 no.4
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    • pp.687-703
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    • 2022
  • This study aims to derive suggestions to help develop Hanbok education in the future by examining its current status in domestic educational institutions in light of rising social demand for Hanbok in the era of fashion Hanbok. We assessed the Hanbok curriculum in educational institutions nationwide, including universities and specialized institutes, and conducted in-depth interviews to understand the operational status of Hanbok-specialized education. We found that the demand for education on production functions is decreasing, while the demand for in-depth education on the history of Korean costume and education integrating Hanbok with design and marketing is increasing. We also found that, in the case of universities, which play an important role in Hanbok research and education, professional research and training are shrinking in graduate schools, whereas Hanbok subjects are being maintained and new subjects are being taught in undergraduate courses. In the fashion Hanbok era, it has been suggested that Hanbok education needs to converge with design and marketing education, beyond teaching only Hanbok. In addition, the necessity of expanding liberal arts education within universities to meet social demand was proposed, and online education was expected to help spread knowledge.

Guidelines for Microbiological Standards of Food in Foreign Countries (제외국의 식품안전관련 미생물 정량기준 가이드라인)

  • Lee, Mi-Seon;Woo, Gun-Jo;Park, Jong-Seok;Lee, Dong-Ha;Oh, Sang-Suk
    • Journal of Food Hygiene and Safety
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    • v.19 no.3
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    • pp.140-150
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    • 2004
  • It has been recognized that international food standards should be considered for a long time. The Codex Alimentarius programme initiated in the early 1960s has received valuable background documentation from the ICMSF (International Commission on Microbiological Specification for Foods) and also from ISO(the International Organization for Standardization). There has been a considerable move towards international harmonization of standards and methods of analysis. Many foreign countries recommend the guidelines for food microbiological standards quantitatively. To complement microbiological limits and tolerance levels for permissible number of defective samples, allowances should be made for sampling and other variations in laboratory methods. It may be necessary for guidelines of domestic food microbiological standards to be established so that the domestic food standards can be harmonized with foreign food standards. Food safety related microbiological guidelines of ICMSF, EU (European Union), UK (United Kingdom), China (Hong Kong) and Japan were reviewed and shown in examples.