• Title/Summary/Keyword: H21

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hEPO 당쇄부위 돌연변이체 제작 및 CHO 세포로부터 변이 단백질의 생산

  • 이풍연;이현기;정희경;이연근;민관식;장원경;이훈택
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.21-21
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    • 2002
  • 사람의 erythropoietin (hEPO) 는 산성 당단백질 호르몬이며 적혈구 생산의 주요조절인자로서 적혈구의 분화와 hemoglobin (Hb) 형성을 촉진하여 빈혈치료제로 이용된다. 사람 EPO 는 166개 아미노산으로 구성되어 있으며, 24, 38, 83 번 아미노산은 N-glycosylation에 의해, 126 번 아미노산은 O-glycosylation에 의해 변형되며, 특히 N-glycosylation은 hEPO 의 세포외 분비 및 활성에 관여한다고 보고된 바 있다. (중략)

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Effect of Onion on Kimchi Fermentation ( I ) (양파가 김치 발효에 미치는 영향( I ))

  • 이진희;이혜수
    • Korean journal of food and cookery science
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    • v.8 no.1
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    • pp.27-30
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    • 1992
  • The changes on the pH and acidity of kimchi with the addition of different amounts of onion were inverstigated during fermentation at $14^{\circ}C$ and $21^{\circ}C$ for 20 days. As the fermentation period increases, the pH of kimchi contained onion was higher than that of control kimchi, and tokal, volatile and non-volatile acidity of kimchi contatined onion were lower than those of control kimchi, at $14^{\circ}C$ and $21^{\circ}C$.

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Morphology and Molecular Phylogeny of Hypnea flexicaulis(Gigartinales, Rhodophyta) from Korea

  • Geraldino, Paul John L.;Yang, Eun-Chan;Bu, Sung-Min
    • ALGAE
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    • v.21 no.4
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    • pp.417-423
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    • 2006
  • Morphology and molecular phylogeny of a red algal species, Hypnea flexicaulis that is recently described from Japan, were investigated based on 23 collections from Korea (21), Taiwan (1), and the Philippines (1). Hypnea flexicaulis has percurrent axes with flexuous, antler-like branches which have wide branching angles, and abaxially curved ultimate branchlets. In order to study DNA divergence and phylogenetic relationships of the species, we determined plastid rbcL and mitochondrial cox1 sequences from the 23 collections. All 21 specimens from five different locations in Korea were almost identical to H. flexicaulis from Japan in rbcL sequences. Although there was a difference of three to five base pairs (bp) between samples from Korea and the Philippines or between the Philippines and Taiwan, Bayesian analyses of the rbcL data showed that all specimens from Korea, Japan, the Philippines, and Taiwan were strongly monophyletic. However, it is interesting that specimens from the Philippines differed by 31-34 base pairs in mitochondrial cox1 gene from those of materials from Korea and Taiwan, which differed by one to seven bp in rbcL between them. Although H. boergesenii is different from H. flexicaulis in having many antler-like branchlets, both appeared as sisters in all analyses of the rbcL data. This is the first report of H. flexicaulis from Korea based on morphology, rbcL, and cox1 gene sequences.

Formation of a New Solo-LTR of the Human Endogenous Retrovirus H Family in Human Chromosome 21

  • Huh, Jae-Won;Kim, Dae-Soo;Ha, Hong-Seok;Kim, Tae-Hong;Kim, Wook;Kim, Heui-Soo
    • Molecules and Cells
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    • v.22 no.3
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    • pp.360-363
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    • 2006
  • Human endogenous retroviruses (HERVs) contribute to various kinds of genomic instability via rearrangement and retrotransposition events. In the present study the formation of a new human-specific solo-LTR belonging to the HERV-H family (AP001667; chromosome 21q21) was detected by a comparative analysis of human chromosome 21 and chimpanzee chromosome 22. The solo-LTR was formed as a result of an equal homologous recombination excision event. Several evolutionary processes have occurred at this locus during primate evolution, indicating that mammalian-wide interspersed repeat (MIR) and full-length HERV-H elements integrated into hominoid genomes after the divergence of Old World monkeys and hominoids, and that the solo-LTR element was created by recombination excision of the HERV-H only in the human genome.

Physiological Characteristics of Symbiotic Fungi Associated with the Seed Germination of Gastrodia elata

  • Hong, In-Pyo;Kim, Han-Kyoung;Park, Jeong-Sik;Kim, Gwang-Po;Lee, Min-Woong;Guo, Shun-Xing
    • Mycobiology
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    • v.30 no.1
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    • pp.22-26
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    • 2002
  • This study was carried out to investigate the possibility for seeds germination of Gastrodia elata using symbiotic fungi. Since seeds of G. elata are very small and lack an endosperm and other nutrients, their germination is difficult without requirement for external nutrients. Out of twenty six isolates collected from protocorms of G. elata and roots of native orchids inhabited in wild, two strains(H-2 and H-21) were observed to stimulate the seed germination of G. elata. The seed germination of G. elates was excellent on oak tree leaves medium. The optimal conditions for mycelial growth of symbiotic fungi were $25^{\circ}C$ and pH 6.0, respectively. The mycelial growth of H-2 strain was excellent on YMA medium, while H-21 was poor on PDA medium. In case of carbon sources, the mycelial growth of H-2 and H-21 was good on media supplemented with glucose and dextrin, respectively. Calcium nitrate was good for mycelial growth of H-2 strain as a nitrogen sources, whereas urea was effective to H-21 strain.

The Characteristics of Hydrogen Permeation through Pd-coated $Nb_{56}Ti_{23}Ni_{21}$ Alloy Membranes (Pd 코팅된 $Nb_{56}Ti_{23}Ni_{21}$ 합금 분리막의 수소투과 특성)

  • Jung, Yeong-Min;Jeon, Sung-Il;Park, Jung-Hoon
    • Membrane Journal
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    • v.22 no.1
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    • pp.1-7
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    • 2012
  • We make a studyof the hydrogen permeability and chemical stability of $Nb_{56}Ti_{23}Ni_{21}$ metal alloy membrane. For this purpose, we produced the $Nb_{56}Ti_{23}Ni_{21}$ membrane which has 10 mm diameter and 0.5 mm thick, and experiment the hydrogen transport properties under two kinds of feed gas ($H_2$ 100%; $H_2$ 60% + $CO_2$ 40%) at $450^{\circ}C$C with variation of absolute pressure.The maximum hydrogen permeation flux was $5.58mL/min/cm^2$ in the absolute pressure 3 bar under pure hydrogen. And each case of feed gases about gas composition, the permeation fluxes were satisfied with Sievert's law, and the hydrogen permeation flux decreased with decrease of hydrogen partial pressure irrespective of temperature and pressure. After permeation test, we experiment the stability and durability of $Nb_{56}Ti_{23}Ni_{21}$ alloy membrane for carbon dioxide by XRD analysis.

Quality Characteristics of Hamburger Bread Prepared by the Addition of Kimchi Homogenate (김치 첨가 햄버거빵의 품질 특성)

  • 김정숙
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.1
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    • pp.34-38
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    • 2004
  • This study was carried out to evaluate the effects of Kimchi homogenate product(KHP) on dough characteristics and quality of bread. Breads were prepared by the addition of 0, 7, 14 and 21% of KHP to the basic formulation. The pH of dough with KHP were ranged from 5.19 to 5.30 and pH of the control was 5.58. Loaf volume index of the bread prepared by adding 7∼21% KHP increased by 5.4∼17.2%. Color L value of the bread crust and crumb with KHP decreased, color a value of the bread crumb with KHP increased, and b value of the crumb with KHP decreased. The hardness decreased with the addition of KHP, but the cohesiveness and springiness increased with the addition of KHP. Sensory quality of the Kimchi hamburger bread estimated by shape, flavor and overall quality was better than that of the control bread. Especially, the Kimchi hamburger bread quality with addition of 14% KHP was the best.

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MR-based Partial Volume Correction for $^{18}$F-PET Data Using Hoffman Brain Phantom

  • Kim, D. H.;Kim, H. J.;H. K. Jeong;H. K. Son;W. S. Kang;H. Jung;S. I. Hong;M. Yun;Lee, J. D.
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.322-323
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    • 2002
  • Partial volume averaging effect of PET data influences on the accuracy of quantitative measurements of regional brain metabolism because spatial resolution of PET is limited. The purpose of this study was to evaluate the accuracy of partial volume correction carried out on $^{18}$ F-PET images using Hoffman brain phantom. $^{18}$ F-PET Hoffman phantom images were co-registered to MR slices of the same phantom. All the MR slices of the phantom were then segmented to be binary images. Each of these binary images was convolved in 2 dimensions with the spatial resolution of the PET. The original PET images were then divided by the smoothed binary images in slice-by-slice, voxel-by-voxel basis resulting in larger PET image volume in size. This enlarged partial volume corrected PET image volume was multiplied by original binary image volume to exclude extracortical region. The evaluation of partial volume corrected PET image volume was performed by region of interests (ROI) analysis applying ROIs, which were drawn on cortical regions of the original MR image slices, to corrected and original PET image volume. From the ROI analysis, range of regional mean values increases of partial volume corrected PET images was 4 to 14%, and average increase for all the ROIs was about 10% in this phantom study. Hoffman brain phantom study was useful for the objective evaluation of the partial volume correction method. This MR-based correction method would be applicable to patients in the. quantitative analysis of FDG-PET studies.

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Novel Lanthanide Coordination Polymers Prepared by Microwave Heating: [Ln(L)3(H2O)2](H2O)3 (Ln = Eu, Tb, Gd; L = trans-(3-py)-CH=CH-COO)

  • Han, Sun-Hwa;Zheng, Zhen Nu;Cho, Sung-Il;Lee, Soon-W.
    • Bulletin of the Korean Chemical Society
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    • v.33 no.6
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    • pp.2017-2022
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    • 2012
  • Three isostructural lanthanide coordination polymers, $[Ln(L)_3(H_2O)_2](H_2O)_3$ {Ln = Eu (1), Tb (2), Gd (3); L = $trans$-3-(3-pyridyl)acrylate, (3-py)-CH=CH-COO}, were prepared from HL, lanthanide nitrate, and NaOH in $H_2O$ by microwave heating. In all coordination polymers, the metal is bonded to eight oxygen atoms, and all pyridyl nitrogen atoms do not coordinate to the metals. All polymers have a 1-D loop-connected chain structure. The hydrogen atoms in the aqua ligands and lattice water molecules all participate in the hydrogen bonds of the O-$H{\cdots}O$ or O-$H{\cdots}N$ type. The hydrogen bonds connect the 1-D chains to create a 2-D network. Polymer 1 exhibited red luminescence in the solid state at room temperature.