• Title/Summary/Keyword: H-Beta

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Cholesterol Removal from Lard with Crosslinked ${\beta}$-Cyclodextrin

  • Kim, S.H.;Kim, H.Y.;Kwak, H.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1468-1472
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    • 2007
  • The present study was carried out to determine the optimum conditions of different factors (ratio of lard to water, ${\beta}$-CD concentrations, mixing temperature, mixing time and mixing speed) on cholesterol reduction from lard by using crosslinked ${\beta}$-CD. Crosslinked ${\beta}$-CD was prepared with adipic acid. When the lard was treated under different conditions, the range of cholesterol removal was 91.2 to 93.0% with 5% crosslinked ${\beta}$-CD, which was not significantly different among treatments. In a recycling study, cholesterol removal with crosslinked ${\beta}$-CD in the first trial was 92.1%, which was similar to that with new crosslinked ${\beta}$-CD. In up to eight time trials, over 90% of cholesterol removal was found. The present study indicated that the optimum conditions for cholesterol removal using crosslinked ${\beta}$-CD were a 1:3 ratio of lard to water, 5% crosslinked ${\beta}$-CD concentration, $40^{\circ}C$ mixing temperature, 1 h mixing time and 150 rpm mixing speed. In addition, crosslinked ${\beta}$-CD made by adipic acid resulted in an effective recycling efficiency.

$H{\alpha}$ - $Ly{\beta}$ Formation in Optically Thick Medium

  • Chang, Seok-Jun;Lee, Hee-Won
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.2
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    • pp.87.2-87.2
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    • 2014
  • Symbiotic stars and quasars exhibit prominent $H{\alpha}$ emission lines often accompanied with broad wings. $H{\alpha}$ emission nebulae in these objects are proposed to be optically thick to resonance scattering. The transfer of $H{\alpha}$ line photons are further complicated by the existence of another scattering channel leading to re-emission of $Ly{\beta}$. In this work are develop a Monte Carlo code to simulate the transfer of $H{\alpha}$ line photons incorporating the scattering channel into $Ly{\beta}$. We show various line profiles of $H{\alpha}$ and $Ly{\beta}$ emergent from our model nebulae. It is shown that temperature is a critical parameter which controls the ratio of emergent $Ly{\beta}$ flux to that of $H{\alpha}$.

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Characterization of Double Transgenic Mice Harboring Both Goat $\beta$-casein/hGH and Goat $\beta$-casein/hG-CSF Hybrid Genes

  • Oh, Keon-Bong;Lee, Chul-Sang
    • Development and Reproduction
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    • v.13 no.3
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    • pp.191-198
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    • 2009
  • In an attempt to simultaneously produce two human proteins, hGH and hG-CSF, in the milk of transgenic mice, we constructed goat $\beta$-casein-directed hGH and hG-CSF expression cassettes individually and generated transgenic mice by co-injecting them into mouse zygotes. Out of 33 transgenic mice, 29 were identified as double transgenic harboring both transgenes on their genome. All analyzed double transgenic females secreted both hGH and hG-CSF in their milks. Concentrations ranged from 2.1 to $12.4\;mg/m{\ell}$ for hGH and from 0.04 to $0.13\;mg/m{\ell}$ for hG-CSF. hG-CSF level was much lower than hGH level but very similar to that of single hG-CSF mice, which were introduced with hG-CSF cassette alone. In order to address the causes of concentration difference between hGH and hG-CSF in milk, we examined mRNA level of hGH and hG-CSF in the mammary glands of double transgenic mice and tissue specificity of hG-CSF mRNA expression in both double and single transgenic mice. Likewise protein levels in milk, hGH mRNA level was much higher than hG-CSF mRNA, and hG-CSF mRNA expression was definitely specific to the mammary glands of both double and single transgenic mice. These results demonstrated that two transgenes have distinct transcriptional potentials without interaction each other in double transgenic mice although two transgenes co-integrated into same genomic sites and their expressions were directed by the same goat $\beta$-casein promoter. Therefore goat $\beta$-casein promoter is very useful for the multiple production of human proteins in the milk of transgenic animals.

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Neural Adaptation of Beta Adrenergic Receptor Subtypes after Chronic Imipramine Treatment: A Quantitative Autoradiographic Study

  • Park, Hae-Young;Hong, Young-Sook;Park, Chan-Woong
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.2
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    • pp.161-167
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    • 1997
  • This study compares the subtypes of central beta adrenergic receptors (ARs) of brains of untreated rats with those of imipramine-treated rats. Beta adrenergic receptors were measured by quantitative autoradiography of the binding of $^3H$-dihydroalprenolol ($^3H$-DHA) in coronal sections of rat brain. Repeated treatment of rats with imipramine significantly reduced the binding of $^3H$-DHA to beta-1 AR in many brain areas, especially throughout the cerebral cortex, hippocampus, thalamus, and amygdala. Significant reductions of the binding of $^3H$-DHA to beta-2 AR were not found in any area of the brain. These data suggests that a selective down-regulation of beta-1 AR may be involved in the adaptive changes occurring after prolonged imipramine treatment.

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ON THE HILBERT SPACE OF FORMAL POWER SERIES

  • YOUSEFI, Bahman;SOLTANI, Rahmat
    • Honam Mathematical Journal
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    • v.26 no.3
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    • pp.299-308
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    • 2004
  • Let $\{{\beta}(n)\}^{\infty}_{n=0}$ be a sequence of positive numbers such that ${\beta}(0)=1$. We consider the space $H^2({\beta})$ of all power series $f(z)=^{Po}_{n=0}{\hat{f}}(n)z^n$ such that $^{Po}_{n=0}{\mid}{\hat{f}}(n){\mid}^2{\beta}(n)^2<{\infty}$. We link the ideas of subspaces of $H^2({\beta})$ and zero sets. We give some sufficient conditions for a vector in $H^2({\beta})$ to be cyclic for the multiplication operator $M_z$. Also we characterize the commutant of some multiplication operators acting on $H^2({\beta})$.

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Influence of Phenobarbital on the Circadian Rhythm of Opiate Receptor in Rat Brain (백서의 뇌내 Opiate 수용체의 일중 변동에 미치는 Phenobarbital의 영향)

  • Park, Yeoung-Gul;Kim, Kee-Won;Cho, Kyu-Park
    • The Korean Journal of Pharmacology
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    • v.21 no.2
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    • pp.128-141
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    • 1985
  • To investigate the influence of phenobarbital sodium on the action of morphine and on the diurnal rhythms of both opiate receptor binding and ${\beta}-endorphin$ contents, the amount of specifically bound $(^3H)$-morphine and immunoreactive ${\beta}-endorphin$ were measured in the midbrain of phenobarbital-treated rats at 4h intervals in a day. Rats were housed and adapted to a controlled cycle of either 12 h light-12 h dark or 24 h constant dark. After 3 weeks of adaptation, 0.5 ml of physiological saline or phenobarbital sodium (20mg/kg/day, i.p.) were administered twice a day for 2 weeks. Highly significant diurnal rhythms of opiate receptor binding and ${\beta}-endorphin$ were present in rat midbrain. In control group, the peak of maximum $(^3H)$-morphine binding was observed at 22:00 h, whereas the peak of ${\beta}-endorphin$ content was found at 06:00 h. Even in the absence of time cues these diurnal rhythms persisted, but they were highly modified with respect to the wave form as well as differences in the timing of peak and nadir. In the phenobarbital-treated group, these diurnal rhythms were also modified in shape, phase and amplitude, as well as in timing of peak and nadir. In this group, 24 h mean of opiate receptor binding was significantly decreased, while the 24 h mean level of ${\beta}-endorphin$ content was highly increased. However, Kd values in all experimental groups did not change. This indicates that differences in binding were not due to changes in the affinity, but in the number of binding sites. Statistical analysis of regression line indicates that changes of receptor binding were closely correlated with the changes of ${\beta}-endorphin$ content. These results suggest that phenobarbital may influence the action of morphine by changing the number of opiate receptors and that the modification of diurnal rhythm of opiate receptor by the agent is possibly due to changes of ${\beta}-endorphin$ content.

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Microstructure and Polytype of in situ-Toughened Silicon Carbide

  • Young Wook Kim;Mamoru Mitomo;Hideki Hirotsuru
    • The Korean Journal of Ceramics
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    • v.2 no.3
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    • pp.152-156
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    • 1996
  • Fine (~0.09 $\mu$m) $\beta$-SiC Powders with 3.3wt% of large (~0.44$\mu$m) $\alpha$-SiC of $\beta$-SiC particles (seeds) added were hotpressed at 175$0^{\circ}C$ using $Y_2O_3$, $Al_2O_3$ and CaO as sintering aids and then annealed at 185$0^{\circ}C$ for 4 h to enhance grain growth. The resultant microstructure and polytypes were analyzed by high resolution electron microscopy (HREM).Growth of $\beta$-SiC with high density of microtwins and formation of ${\alpha}/{\beta}$ composite grains consisting of $\alpha$-SiC domain sandwiched between $\beta$-SiC domains were found in both specimens. When large $\alpha$-SiC (mostly 6H) seeds were added, the $\beta$-SiC transformend preferentially to the 6H polytype. In contrast, when large $\beta$-SiC (3C) seeds were added, the fine $\beta$-SiC transformed preferentially to the 4H polytype. Such results suggested that the polytype formation in SiC was influenced by crystalline form of seeds added as well as the chemistry of sintering aids. The ${\alpha}/{\beta}$ interface played and important role in the formation of elongated grains as evidenced by presence of ${\alpha}/{\beta}$ composite grains with high aspect ratio.

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Purification and Characteristics of ${\beta}$-Galactosidase from Strawberry (딸기 ${\beta}$-Galactosidase의 정제 및 생화학적 특성)

  • Lee, Kwang-Hee;Yoon, Kyung-Young;Kim, Kwang-Soo;Kim, Nam-Woo;Shin, Seung-Ryeul
    • Food Science and Preservation
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    • v.7 no.2
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    • pp.201-206
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    • 2000
  • ${\beta}$-Galactosidase was extracted and purified from strawberry. The purified ${\beta}$-Galactosidase from strawberry was investigated their physicochemical characteristics. ${\beta}$-Galactosidase was purified 25.74 fold from strawberry. The purification procedure include ammonium sulfate fraction, acetone powder treatment and gel and ion exchange chromatography. Yield of the enzyme purification was 18.11%. The purified enzyme has native molecular weight of 116,000 dalton. Vmax value and Km value of ${\beta}$-Galactosidase were 0.077 mM ONPG/ml/15mim and 1.75x10-2mM, respectively. The optimum temperature and pH of ${\beta}$-Galactosidase were 43$^{\circ}$C and pH 4.0, respectively. The ${\beta}$-Galactosidase activity was stable below 50$^{\circ}$C and at pH 4.0 to pH 6.0. Among the metal ions Ca and Mg were did not affect, whereas K, Cu and Zn show a little effect on the enzyme activity. The ${\beta}$-Galactosidase activities were inhibited by treatment with EDTA and SDS.

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Predictive Value of Serum ${\beta}-hCG$ Level in Pregnancies following In vitro Fertilization and Embryo Transfer (체외수정시술 후 임신된 환자에서 혈중 ${\beta}-hCG$ 측정에 의한 임신 결과 예측에 관한 연구)

  • Kim, Seok-Hyun;Suh, Chang-Suk;Choi, Doo-Seok;Choi, Young-Min;Shin, Chang-Jae;Kim, Jung-Gu;Moon, Shin-Yong;Lee, Jin-Yong;Chang, Yoon-Seok
    • Clinical and Experimental Reproductive Medicine
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    • v.19 no.1
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    • pp.41-48
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    • 1992
  • Serum level of ${\beta}$ subunit of human chorionic gonadotropin (${\beta}-hCG$) was studied to evaluate its predictability of pregnancy outcome in 98 in vitro fertilization and embryo transfer(IVF-ET) patients using gonadotropin-releasing hormone(GnRH) agonist. Serial serum ${\beta}-hCG$ levels were established for 42 singleton pregnancies, 20 normal multiple pregnancies, 18 preclinical abortions, 14 clinical abortions and 4 ectopic pregnancies. In comparison to normal singleton pregnancies, multiple pregnancies showed significantly higher ${\beta}-hCG$ levels on the post-ET day 10 to 13 and day 24 to 25. Clinical abortions did not show significantly lower ${\beta}-hCG$ levels in early pregnancy except the post-ET day 16-17, but showed significantly lower ${\beta}-hCG$ levels from the post-ET day 22, compared with singleton pregnancies. Preclinical abortions showed significantly lower ${\beta}-hCG$ levels than those of singleton pregnancies. Ectopic pregnancies showed lower ${\beta}-hCG$ levels than those of singleton pregnancies without statistical significance. In conclusion, determination of serum ${\beta}-hCG$ level in early pregnancy is a useful tool for the prediction of preclinical abortions and multiple pregnancies and serial measurement of serum ${\beta}-hCG$ levels will be helpful in predicting clinical abortion.

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Immobilized ${\beta}-Cyclodextrin$ as a Simple and Recyclable Method for Cholesterol Removal in Milk

  • Kwak, H.-S.;Kim, S.-H.;Kim, J.-H.;Choi, H.-J.;Kang, J.
    • Archives of Pharmacal Research
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    • v.27 no.8
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    • pp.873-877
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    • 2004
  • This study was designed to determine the optimum conditions of three different factors (mixing time, mixing temperature, and tube size) in reduction of cholesterol in milk using immobilized $\beta$-CD beads. Immobilized $\beta$-CD glass beads were prepared at different conditions of silaniza-tion and $\beta$-CD immobilization reactions. In result, the glass beads (diameter 1 mm) at 20 mM 3-isocyanatopropyltriethoxysilane and 30 mM $\beta$-CD without base showed the highest choles-terol removal rate as 41%. Using above immobilized $\beta$-CD glass beads, the cholesterol removal rate was 40.2% with 6 h of mixing time in 7 mm diameter tube at $10^{\circ}C$. After choles-terol removal from milk, the glass beads were washed for cholesterol dissociation and reused. In recycling study, the cholesterol removal rate was 41%, which was mostly same as that using new glass beads. These results indicated that cholesterol removal rate was about 40% with $\beta$-CD immobilized glass beads, however, the recycling efficiency was almost 100%.