• Title/Summary/Keyword: Growing rod

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Characterization of a New Acidophilic Acetobacter sp. Strain HA Isolated from Korean Traditional Fermented Vinegar

  • CHUN, HONG-SUNG;SUNG-JUN KIM
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.108-114
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    • 1993
  • A new strain of acidophilic, acetogenic bacterium, Acetobacter sp. strain HA was isolated by selective enrichment from the traditionally fermented rice wine vinegar in Korea. It was a gram-negative, non-motile short rod and oxidized acetate and lactate. The optimal temperature and pH for growth were $28^{\circ}C$ and 4.0, respectively. The strain HA differed from other Acetobacter species by growing well on methanol, xylitol, inositol, dulcitol, D-xylose, L-arabinose, and D-mannose as sole sources of carbon and energy. The isolated strain HA did not produce $\gamma$-pyrones from glucose and did not produce ketone bodies from glycerol. The quinone system used in this study was an ubiquinone-9 isoprene unit. The guanine-plus-cytosine content of the DNA was 50.7 mol%, and the major cellular fatty acids were $C_{18:1} and C_{16:0}$.

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Electrospun TiO2 Electrodes for Quasi-Solid State Dye- sensitizedSolar Cell

  • Song, Mi-Yeon;Ahn, Young-Rack;Jo, Seong-Mu;Kim, Dong-Young
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.65-66
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    • 2006
  • [ $TiO_2$ ] single crystalline nanorods are prepared from electrospun fibers which are composed of nanofibrils with an island-in-a-sea morphology. The mechanical pressure produces each fibril into nanorods which are converted to anatase single crystals after calcinations. HRTEM shows that the (001) plane is growing along the longitudinal direction of the rod. In this work, the nanorod electrode provides the efficient photocurrent generation in a quasi-solid state dye-sensitized solar cells (DSSCs) using highly viscous PVDF-HFP based gel electrolytes. The overall converision efficiency of the $TiO_2$ nanorods shows 6.2 % under $100\;mW/cm^2$ (AM 1.5G) illumination.

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The Viruses in Gladiolus hybridus cultivated in Korea 2. Broad Bean Wilt Virus, Cucumber Mosaic Virus and Tobacco Rattle Virus (한국산 글라디올러스에 발생하는 바이러스)

  • 박인숙;김규원;권현정;장무웅
    • Korean Journal Plant Pathology
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    • v.14 no.1
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    • pp.83-91
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    • 1998
  • Gladioli (Gladiolus hybridus) showing flower colour breaking leaf mosaics, notched leaf, and dwarfing or lack of visible symptoms were collected from gladioli growing areas in Taegu and Kyungpook province, Korea. The three viruses isolated from the naturally infected gladioli were identified as broad bean wilt virus (BBWV), cucumber mosaic virus (CMV), and tobacco rattle virus (TRV) by their host range, immunosorbent electron microscopy (ISEM), enzyme-linked immunosorbent assay (ELISA), direct tissue blotting immunoassay (DTBIA), and intracellural symptoms. By DTBIA and ISEM, TRV was detected in gladiolus showing notched leaf, while CMV was frequently detected in gladioli with dwarfing, color breaking and malformation of flowers. BBWV was also often detected in many symptomless gladiolus plants, but TRV was detected in notched-leaf of gladiolus. Electron microcopic examination of negatively stained preparations showed that BBWV and CMV are spherical particles of 28 nm and 30 nm in diameter, and TRV is rigid rod-shaped particles of 40∼200 nm in length. The rigid rodshaped virus particles reacted positively with TRV antiserum in ISEM and DTBIA, respectively.

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Development of A Automatic Transplanter for Bedding Plants Between Tray (육묘상자간 자동 육묘 이식 시작기 개발)

  • 류관희;한재성;류찬석;김기영
    • Journal of Biosystems Engineering
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    • v.25 no.1
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    • pp.19-24
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    • 2000
  • This study was carried out to develop gripper which to adaptive variable tray and to develop automatic transplanting system for seedling-production system between tray. This system consisted of five set of gripper and end-effector, a planting-width control unit, a tray transfer unit, and gripper moving device which move gripper between nursing tray and growing tray. This system used push-out rod to grasp plant instead of pull-out end -effector. Several types of fingers, which physically grip seedlings, were also developed and tested to ensure reliable transplanting operation of the gripper. The transplanting system detaches seedlings from a tray with push-o0ut rods, which were installed under the tray transfer unit. The performance of the transplanting system was evaluated by successive transplanting experiments. Using the best type of finger , the transplanting system produced 94.6% of transplanting success rate.

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A Study on the Corona Discharge Simulation Using FEM-FCT Method (FEM-FCT 기법을 이용한 코로나 방전 시뮬레이션에 대한 연구)

  • Min, Ung-Gi;Kim, Hyeong-Seok;Lee, Seok-Hyeon;Han, Song-Yeop
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.3
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    • pp.200-208
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    • 1999
  • In this paper, the corona discharge is analyzed by Finite Element Method(FEM) combined with Flux-corrected Transport(FCT) algorithm. In the previous papers, Finite Difference Method(FDM) combined with FCT was used. Usually in the FDM, the regionof interest is discretized with structured grids. But to refine local regions with same resolution, much more grids are required for the structured grids than for unstructured grids than for unstructured grids. Therefore, we propose the FEM-FCT method to simulate the corona discharge. The proposed method has good flexibility in model shape and can reduce the computational cost by the local refinement where the physical quantities have steep gradients. Using the proposed method, we study the streamer growth of parallel plate electrodes which is initiated by the low and high perturbation density. We find that the varying the initial density of perturbation has very little effect on the streamer propagation. And the corona discharge of the rod-to-plane electrode is simulated. On the surface of the rod electrode, the high concentration of the electric field gives rise to many number of streamer seeds. The strong axial streamer propagate to the plane electrode. The weaker non-axial streamer repel each other and stop growing more. The results are very similar to those of the papers which used the FDM-FCT method on structured grids. Thus we can conclude that the proposed FEM-FCT method is more efficient than the conventional FDM-FCT method by virtue of the reduction in computational grids number.

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A study on the optical properties of $LiNbO_3$ single crystal grown by Floating zone method (Floating zone 법에 의한 $LiNbO_3$ 단결정의 광학적 특성에 관한 연구)

  • Ko, J.M.;Cho, H.;Kim, S.H.;Choi, J.K.;Auh, K.H.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.5 no.4
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    • pp.318-331
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    • 1995
  • The c - axis oriented single crystal of $LiNbO_3$ and $LiNbO_3$ : 5mol%MgO was success-fully grown by Floating zone method using halogen lamp as a heat source. The effects of the sintering condition of the feed rod and the atmosphere gas during the crystal growth on the be havior of the feed rod/melt interface were studied for growing crystal with the high quality, and then, the optimum growth conditions were determined by studying the experimental param eters, such as gas flow rate, pulling rate, rotation speeds of the feed rod and the seed. The grown crystals were analyzed using the chemical etching to observe the tch pattern and the ICP (Inductively Coupled Plasma) to determine the composition uniformity and the impurity content of Fe. The effects of additive (5 mol % MgO) on the transmittance and refractive index was, also, analyzed. In order to compare the nonlinear optical oharacteristics of $LiNbO_3$ with those of the other optical materials, the nonlinear optical refractive index ($n_2$) was calcu l lated using the measured refractive index.

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Selective Dissolution of ZnO Crystal by a Two-step Thermal Aging in Aqueous Solution (수용액 합성법의 2단계 성장온도 변화를 통한 ZnO 결정의 선택적 용해 현상)

  • Kim, Jeong-Seog;Chae, Ki-Woong
    • Journal of the Korean Ceramic Society
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    • v.48 no.4
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    • pp.263-268
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    • 2011
  • ZnO hexagonal rods grown in aqueous solution can be changed into a tubular shape by two-step aging in the course of the growing process. In the first step, hexagonal ZnO rods is grown by aging at $90^{\circ}C$ under a highly supersaturated aqueous solution giving rise to a fast precipitation rate. Meanwhile, during the second step aging at $60^{\circ}C$ in the same aqueous solution, the hexagonal polar face (001) having higher surface energy than (010) side planes dissolves to minimize surface energy. Hence the flat (001) face changes to a craterlike face and the hexagonal rod length of ZnO decreases at an initial-stage of this step aging. The formation of the (101) wedge-type faces is ascribed to the resultant of competitive reactions between the dissolution of polar face minimizing the surface energy which is a dominant reaction at the initial stage and the precipitation reaction dissipating supersaturation. At a later stage of the second-step the reaction rates of these two processes in the aqueous solution become similar and the overall reaction is terminated.

GROWTH AND CHARACTERIZATION OF $La_3Ga_5SiO_{14}$ SINGLE CRYSTALS BY THE FLOATING ZONE METHOD

  • Yoon, Won-Ki;Auh, Keun-Ho
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1999.06a
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    • pp.253-269
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    • 1999
  • The development of telecommunication and information technology requires to develop new piezoelectric materials with small size, low impedance, wide pass band width and high thermal stability of frequency. Langasite (La3Ga5SiO14) single crystal has been researched substitute of quartz and LiNbO3 for the applications of SAW filter, BAW filter and resonator. Its single crystal growth has been carried out by Czochralski Method. So, in order to get single crystal with higher quality, in this study, lnagasite (La3Ga5SiO14) single crystal was grown by using Floating Zone (FZ) method and characterized. For the growth of langasite single crystals, the langasite powder was synthesized at 135$0^{\circ}C$ for 5hrs and the feed rod was sintered at 135$0^{\circ}C$ for 5hrs. The growing rate was 1.5mm/h and the rotation speed was 15 rpm for an upper rotation and 13 rpm for a lower rotation. In order to prevent the evaporation of gallium oxide, Ar and O2 gas mixture was flowed. The growth direction was analyzed by Laue back-scattered analysis. The composition of grown crystal was analyzed suing XRD and WDS. The electrical properties of grown crystal at various frequencies and temperature were discussed.

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Identification and Characterization of Hemolytic Bacillus cereus Isolated from Commercial Ssam-jang (시판 쌈장에서 분리한 용혈성 Bacillus cereus의 동정 및 특성 조사)

  • Kim, Dong-Min;Park, Sang-Kook;Oh, Kye-Heon
    • KSBB Journal
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    • v.32 no.3
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    • pp.179-186
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    • 2017
  • This study was undertaken to identify and characterize hemolytic Bacillus cereus isolated from commercial ssam-jang. The physiological and biochemical properties of isolate were first examined. Using the BIOLOG system, the isolate was identified and assigned to B. cereus MH-2. Phylogenetic tree of MH-2 was plotted based on 16S rRNA sequence comparisons. Hemolytic activity was observed around wells of sheep blood agar plates seeded with MH-2 cultures; the zone of hemolysis gradually increased with increasing incubation time of the cultures. Zymographic analysis estimated the molecular weight of the presumed hemolysis-causing molecule to be about 30 kDa. Survival rates of MH-2 cells decreased with increasing NaCl concentrations in the media. The stress shock proteins (e.g., DnaK and GroEL) induced by NaCl were reduced in proportion to the NaCl concentration and exposure period to B. cereus MH-2. Analysis of SDS-PAGE and Western blot revealed that the stress shock proteins, 70-kDa DnaK and 60-kDa GroEL were decreased proportionate to the NaCl concentrations as well as exposure period in exponentially growing cultures. Scanning electron microscopy demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with NaCl.

Current Status of Bacterial Brown Stripe of Rice Caused by Acidovorax avenae subsp. avenae (Acidovorax avenae subsp. avenae에 의한 세균성줄무늬병의 연구동향)

  • 송완엽
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.69-76
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    • 1999
  • Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

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