• Clinical and Experimental Reproductive Medicine
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• 제18권1호
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• pp.35-40
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• 1991

To find out the suitable method for blastomeres fusion of mouse 2-cell embryo using electric stimuli, these studies were carried out with various voltages (1.0 KV, 1.2 KV, 1.5 KV, 1.7 KV and 2.0KV), pulse duration times($50{\mu}\;sec$, $75/{\mu}\;sec$, $100{\mu}\;sec$) and different fusion solutions. In addition, the fused embryos were cultured for 72-80hr to observe their subsequent development. These results were summarized as follows: 1. The proportion of the fused embryos were 50.8%(34/67), 60.7%(34/56), 70.6%(48/68), 66.7% (48/72) and 85.3% (58/68) after stimuli of 1.0KV, 1.2KV, 1.5KV, 1.7KV and 2.0KV for $100{\mu}\;sec$ with 2 times, and the electric stimulation at 2.0KV(85.3%) was the most effective voltage on the blastomere fusion. 2. For in vitro development, blastocysts of the fused embryos were cultured for 72-80hrs in $M_{16}$ medium. The group(52.1%) treated with 1.5KV for $100{\mu}\;sec$ with 2 times showd higher development rates than those any other group. However, these results were not corresponded to those of the rates of blastomere fusion. 3. There were no significant differences among the rates of blastomeres fusion to 50(70.6%), 75(71.9%), and 100(78.0%) ${\mu}sec$ stimulation at 1.5KV with two times. However, the development rates of the fused embryo in vitro were 52.1%(25/48), 28.3%(13/46) and 9.4%(3/32) at the above conditions, and the development rates of fused embryo increased as the pulse duration times increased. 4. The rates of the blastomeres fusion were 38.9% (28/72) or 70.6% (48/68) in electrolyte (PBS) or non-electrolyte(0.3M mannitol) solution. The development rates of the fused embryo were 32.1% (9/28) or 52.1%(25/48) in the above fusion solutions, and non-electrolyte-treated group showed higher development rates of embryo than that of electrolyte-treated group.

• Journal of Korean Neurosurgical Society
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• 제30권2호
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• pp.194-200
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• 2001

Object : Anterior cervical discectomy and fusion is accepted as appropriate surgical intervention for disease processes of the ventral cervical spine. In some cases, however, improvement of symptom following operation develop myelopathy or rhizopathy at longterm follow-up. We studied to clarify the correlation between clinical results and plain radiologic findings at long term follow-up. Methods : A total of 86 patients who underwent anterior cervical discectomy and fusion were grouped into deteriorated and good recovery group. These two groups were compared in lateral functional roentgenograms on the cervical kyphosis and disc height at fused level, sagittal plane rotation and dynamic sagittal canal diameter at adjacent level. Results : The presence of cervical kyphosis and disc height at fused level do not correlate with long term followup results. Sagittal plane rotation of more than 20 degrees were identified in 36% of the cases in deteriorated group, whereas the same findings were identified in 15% of the cases in good group. Sagittal diameter of less than 12mm were identified in 48% of the cases in deteriorated group, whereas the same finding were identified in 8% of the cases in good group. Preoperatively, sagittal plane rotation of more than 20 degrees at adjacent level were identified in 28% of the cases in deteriorated group, whereas the same findings were identified in 13% of the cases in good group. Preoperatively sagittal diameter of less than 12mm at adjacent level were identified in 40% of the cases in deteriorated group, whereas the same finding were identified in 7% of the cases in good group. Conclusion : Large sagittal plane rotation and small dynamic sagittal diameter at adjacent level were factors that might be associated with later clinical deterioration after cervical anterior fusion.

• Bulletin of the Korean Chemical Society
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• 제29권3호
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• pp.647-655
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• 2008

Microsomal prostaglandin E2 synthase (mPGES-1) is a potent target for pain and inflammation. Various QSAR (quantitative structure activity relationship) analyses used to understand the factors affecting inhibitory potency for a series of MK886 analogues. We derived four QSAR models utilizing various quantum mechanical (QM) descriptors. These QM models indicate that steric, electrostatic and hydrophobic interaction can be important factors. Common pharmacophore hypotheses (CPHs) also have studied. The QSAR model derived by best-fitted CPHs considering hydrophobic, negative group and ring effect gave a reasonable result (q2 = 0.77, r2 = 0.97 and Rtestset = 0.90). The pharmacophore-derived molecular alignment subsequently used for 3D-QSAR. The CoMFA (Comparative Molecular Field Analysis) and CoMSIA (Comparative Molecular Similarity Indices Analysis) techniques employed on same series of mPGES-1 inhibitors which gives a statistically reasonable result (CoMFA; q2 = 0.90, r2 = 0.99. CoMSIA; q2 = 0.93, r2 = 1.00). All modeling results (QM-based QSAR, pharmacophore modeling and 3D-QSAR) imply steric, electrostatic and hydrophobic contribution to the inhibitory activity. CoMFA and CoMSIA models suggest the introduction of bulky group around ring B may enhance the inhibitory activity.

• Journal of Korean Neurosurgical Society
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• 제63권6호
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• pp.794-805
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• 2020

Objective : Teriparatide is known as an effective anabolic agent not only for severe osteoporosis but also for bone healing and union. We explored the possibility of teriparatide as an alternative treatment option for osteoporotic thoracolumbar (TL) burst fracture. Methods : This retrospective study enrolled 35 female patients with mean age of 73.77±6.71 years (61-88) diagnosed as osteoporotic TL burst fracture with ≥4 of thoracolumbar injury classification and severity (TLICS) score and no neurological deficits. All patients were treated by teriparatide only (12 of group A), teriparatide plus vertebroplasty (12 of group B), or surgical fixation with fusion (11 of group C), and followed up for 12 months. Radiological outcomes were evaluated using radiological parameters including kyphotic angle (KA), segmental vertebral kyphotic angle (SVKA), compression ratio (CR), and vertebral body height (anterior [AH], middle [MH], posterior [PH]). Functional outcomes were evaluated using visual analog scale (VAS) and Macnab classification (MC). Results : There were no statistical significant differences in age, bone mineral density (-3.36±0.73), and TLICS score (4.34±0.48) among the three groups (p>0.05). Teriparatide was administered during 8.63±2.32 months in group A and B. In 12-month radiological outcomes, there were significant restoration in SVKA, CR, AH, and MH of group B and KA, SVKA, CR, AH, and MH of group C compared to group A with no radiological changes (p<0.05). All groups showed similar significant improvements in 12-month functional outcomes, although group B and C showed a better 1-month VAS, 1-month MC, 3-month MC compared to group A (p<0.05). Conclusion : Non-surgical treatment with teriparatide showed similar 12-month functional outcomes compared to surgical fixation with fusion. The additional vertebroplasty to teriparatide and surgical fixation with fusion were more helpful to improve short-term functional outcomes with structural restoration compared to teriparatide only.

• Journal of Plant Biology
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• 제39권3호
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• pp.209-214
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• 1996

Cytological characters of ten taxa of the genus Lycoris were investigated to illustrate their chromosomal evolution. The fusion theory was more appropriate than the fission theory. From the viewpoint of the fusion theory L. sanguinea group, diploids with R-shaped chromosomes was the most primitive and they might have diverged into two directions, the diploids with V＋R shaped chromosomes and the triped with V＋R shaped chromosomes via triploids with only R-shaped chromosomes.

• Journal of Korean Neurosurgical Society
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• 제45권4호
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• pp.213-218
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• 2009

Objective : This study is a retrospective clinical study over more than 4 years of follow up to understand the mechanism of load sharing across the graft-bone interface in the static locking plate (SLP) fixation compared with non-locking plate (NLP). Methods : Orion locking plates and Top non-locking plates were used for SLP fixation in 29 patients and NLP fixation in 24 patients, respectively. Successful interbody fusion was estimated by dynamic X-ray films. The checking parameters were as follows : screw angle (SA) between upper and lower screw, anterior and posterior height of fusion segment between upper and lower endplate (AH & PH), and upper and lower distance from vertebral endplate to the end of plate (UD & LD). Each follow-up value of AH and PH were compared to initial values. Contributions of upper and lower collapse to whole segment collapse were estimated. Results : Successful intervertebral bone fusion rate was 100% in the SLP group and 92% in the NLP group. The follow-up mean value of SA in SLP group was not significantly changed compared with initial value, but follow-up mean value of SA in NLP group decreased more than those in SLP group (p=0.0067). Statistical analysis did not show a significant difference in the change in AH and PH between SLP and NLP groups (p>0.05). Follow-up AH of NLP group showed more collapse than PH of same group (p=0.04). The upper portion of the vertebral body collapsed more than the lower portion in the SLP fixation (p=0.00058). Conclusion : The fused segments with SLP had successful bone fusion without change in initial screw angle, which was not observed in NLP fixation. It suggests that there was enough load sharing across bone-graft interface in SLP fixation.

• Molecular & Cellular Toxicology
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• 제3권2호
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• pp.145-149
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• 2007

Studies of relationship between structure and toxicity of azo dyes have been performed with comparative molecular field analysis (CoMFA) techniques. 3D QSTR analyses indicate that the steric and electrostatic interactions are important. The steric field based model gives strong correlation ($q^2$=0.57, $r^2$= 0.92). The steric field in conjunction with electrostatic field give more strong correlation ($q^2$=0.57, $r^2$=0.95). All study indicates that a bulky and electronegative group at benzene ring and a small group at position 3 of aniline ring might be significant to reduce the mutagenicity.

• Bulletin of the Korean Chemical Society
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• 제30권2호
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• pp.471-474
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• 2009

• Journal of Korean Neurosurgical Society
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• 제49권6호
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• pp.345-350
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• 2011

Objective : It is debatable whether an anterior oblique fracture orientation is really a contraindication to anterior odontoid screw fixation. The purpose of this study was to investigate the feasibility of anterior odontoid screw fixation of type II and rostral shallow type III fracture with an anterior oblique fracture orientation. Methods : The authors evaluated 16 patients with type II and rostral shallow type III odontoid fracture with an anterior oblique fracture orientation. Of these 16 patients, 8 (group 1) were treated by anterior odontoid screw fixation, and 8 (group 2) by a posterior C1-2 arthrodesis. Results : Of the 8 patients in group 1, seven patients achieved solid bone fusion (87.5%), and one experienced screw back-out of the C-2 body two months after anterior screw fixation. All patients treated by posterior C1-C2 fusion in group 2 achieved successful bone fusion. Mean fracture displacements and fracture gaps were not significantly different in two groups. (p=0.075 and 0.782). However, mean fracture orientation angles were $15.3{\pm}3.2$ degrees in group 1, and $28.6{\pm}8.1$ degrees in group 2 (p=0.002), and mean fragment angulations were $3.2{\pm}2.1$ degrees in group 1, and $14.8{\pm}3.7$ degrees in group 2 (p=0.001). Conclusion : Even when the fracture lines of type II and rostral shallow type III fractures are oriented in an anterior oblique direction, anterior odontoid screw fixation can be feasible in carefully selected patients with a relatively small fracture orientation angle and relatively small fragment angulation.

• Bulletin of the Korean Chemical Society
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• 제31권2호
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• pp.503-506
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• 2010