• Horticultural Science & Technology
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• v.29 no.2
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• pp.87-94
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• 2011

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 $mg{\cdot}L^{-1}$ $GA_3$ and $GA_{4+7}$ solution maintained at $35^{\circ}C$ for two hours (hot water treatment) and/or exposed at $4^{\circ}C$ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at $20^{\circ}C$ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, $GA_{4+7}$ hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, $GA_{4+7}$ hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot $GA_{4+7}$ solution or chilled at $4^{\circ}C$ and shoot emergence rate was highest in bulblets soaked in hot $GA_{4+7}$ solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. cernuum and tsingtauense.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.1
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• pp.15-24
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• 2015

$CH_4$ is a trace gas and one of the key greenhouse gases, which requires continuous and systematic monitoring. The application of eddy covariance technique for $CH_4$ flux measurement requires a fast-response, laser-based spectroscopy. The eddy covariance measurements have been used to monitor $CO_2$ fluxes and their data processing procedures have been standardized and well documented. However, such processes for $CH_4$ fluxes are still lacking. In this note, we report the first measurement of $CH_4$ flux in a rice paddy by employing the eddy covariance technique with a recently commercialized wavelength modulation spectroscopy. $CH_4$ fluxes were measured for five consecutive days before and after the rice transplanting at the Gimje flux monitoring site in 2012. The commercially available $EddyPro^{TM}$ program was used to process these data, following the KoFlux protocol for data-processing. In this process, we quantified and documented the effects of three key corrections: (1) frequency response correction, (2) air density correction, and (3) spectroscopic correction. The effects of these corrections were different between daytime and nighttime, and their magnitudes were greater with larger $CH_4$ fluxes. Overall, the magnitude of $CH_4$ flux increased on average by 20-25% after the corrections. The National Center for AgroMeteorology (www.ncam.kr) will soon release an updated KoFlux program to public users, which includes the spectroscopic correction and the gap-filling of $CH_4$ flux.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.1
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• pp.8-14
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• 2006

High phosphate accumulations in greenhouse soils have been considered as a new agricultural problem in Korea. The effects of silicate on changes in phosphate fractions and on the yield of Chinese cabbage without P fertilization were investigated by pot experiment. For this experiment, P-accumulated soil was selected (Total-P; $2140mg\;kg^{-1}$). Three levels of silicate (0, 2, and $4Mg\;ha^{-1}$) without P fertilization and P fertilizer without silicate application (Si0+NPK) were applied in 1/2000a pots. The same amount of nitrogen and potassium fertilizers were applied to the all pots. The application of $4Mg\;ha^{-1}$ of silicate greatly increased the yield of Chinese cabbage by 25% compared to Si0+NPK treatment. Although there is no significant difference in plant P absorption among all the treatments, the uptake of P in the $4Mg\;ha^{-1}$ silicate application was significantly higher than Si0+NPK treatment due to increase in yield. The content of available $SiO_2$ in soil increased with increasing silicate application rates. The Si concentration of plant showed a positive correlation with available $SiO_2$ contents in soil and the yield of Chinese cabbage. Total P greatly decreased with increasing rates of silicate application, yet the change in available P content was not significant. The Si0+NPK treatment increased the content of Ca-P by 11%, however, which was decreased by 27% in the $4Mg\;ha^{-1}$ silicate application. Therefore, the effect of silicate on reducing total-P was mainly attributed to the change in concentration of Ca-P. Our results suggest that the application of silicate in P-accumulated soils not only increase the crops yield but also reduces phosphate accumulation.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.353-362
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• 2010

The contents of functional and nutritional components of 13 cultivars of Chinese cabbage (CC, $Brassica$ $rapa$ subspecies $campestris$) were analyzed to compare the effects of soil pH of the greenhouse (pH 6.2) and outdoor (pH 7.6). The CC cultivated on pH 6.2 (CC-6.2) soil contained significantly increased amounts (2-9 fold) of pectin, crude protein, vitamin C and vitamin E compared to the counterpart (CC-7.6). The contents of ash and the minerals (Ca, Fe, Na, and Mn) were also significantly increased in CC-6.2. However, CC-6.2 contained 40-50% lower contents of reducing sugars, cellulose and crude fat than CC-7.6. CC-7.6 contained more glucosinolates, gluconasturtiin (18.33 vs. $1.16nmol{\cdot}g^{-1}$ wet weight) and gluconapin (145 vs. $2nmol{\cdot}g^{-1}$ wet wt), than CC-6.2. In conclusion, CC-6.2 had an improved texture (high pectin and low cellulose) and nutritional value (high in protein, Ca, Fe, vitamin C, and E), whereas the CC-7.6 had better taste (high in reducing sugars) and anticancer functionality (high in glucosinolates).

• Journal of Korea Water Resources Association
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• v.52 no.11
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• pp.917-929
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• 2019

Denitrification in streams is of great importance because it is essential for amelioration of water quality and accurate estimation of $N_2O$ budgets. Denitrification is a major biological source or sink of $N_2O$, an important greenhouse gas, which is a multi-step respiratory process that converts nitrate ($NO_3{^-}$) to gaseous forms of nitrogen ($N_2$ or $N_2O$). In aquatic ecosystems, the complex interactions of water flooding condition, substrate supply, hydrodynamic and biogeochemical properties modulate the extent of multi-step reactions required for $N_2O$ flux. Although water flow in streambed and residence time affect reaction output, effects of a complex interaction of hydrodynamic, geomorphology and biogeochemical controls on the magnitude of denitrification in streams are still illusive. In this work, we built a two-dimensional water flow channel and measured $N_2O$ flux from channel sediment with different bed geomorphology by using static closed chambers. Two independent experiments were conducted with identical flume and geomorphology but sediment with differences in dissolved organic carbon (DOC). The experiment flume was a circulation channel through which the effluent flows back, and the size of it was $37m{\times}1.2m{\times}1m$. Five days before the experiment began, urea fertilizer (46% N) was added to sediment with the rate of $0.5kg\;N/m^2$. A sand dune (1 m length and 0.15 m height) was made at the middle of channel to simulate variations in microtopography. In high- DOC experiment, $N_2O$ flux increases in the direction of flow, while the highest flux ($14.6{\pm}8.40{\mu}g\;N_2O-N/m^2\;hr$) was measured in the slope on the back side of the sand dune. followed by decreases afterward. In contrast, low DOC sediment did not show the geomorphological variations. We found that even though topographic variation influenced $N_2O$ flux and chemical properties, this effect is highly constrained by carbon availability.

• Journal of the Korea Organic Resources Recycling Association
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• v.26 no.4
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• pp.11-19
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• 2018

This study was conducted to develop the manufacturing method of biochar using pruned stems of pear tree and its application effect on the crop growth and soil physico-chemical properties. In this study, biochar derived from pruned stems of pear tree at heating temperature of $300^{\circ}C$, $500^{\circ}C$ and $700^{\circ}C$ in heating times of 2, 3 and 4 hours, were tested in the changes of their chemical properties during biochar processing. The pH, Exch. K, Exch. Mg and cation exchange capacity (CEC) increased as the pyrolysis temperature increased during the production of biochar, and the change of these properties rapidly occurred at $500^{\circ}C$. However, as the pyrolysis temperature increased, ash content increased and total carbon (T-C), yield decreased. And the change of the properties in response to the heating time was not shown. It was thought that it would be desirable to set the production conditions of biochar at $500^{\circ}C$ for 2 hours in consideration of the change of chemical properties and the ash content and yield. And also, were conducted the experiments to establish manufacturing method of farm-made biochar using drum biochar manufacturing machine and investigate the application effects of biochar on the cultivation of chinese cabbage and tomato. Application of biochar derived from pruned stems of pear tree could enhance pH, organic matter (OM), total carbon (T-C) of soil. On the other hand, soil electrical conductivity (EC), NO3-N were lowered compared to the control which has no application. The bulk density, porosity and aggregate formation of soil were improved by biochar application. The fresh matter yields of chinese cabbage and tomato were significantly increased in proportion to the application rate of biochar. This study demonstrated the effect of the biochar derived from agricultural byproduct to be as a low cost potential soil ameliorant by physico-chemical properties in eco-friendly greenhouse cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.4
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• pp.276-284
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• 2023

Nitrogen is a major and essential macronutrient for plant growth and development. However, excessive nitrogen application can lead to ecological pollution or greenhouse gas emissions, consequently resulting in climate change. In this study, we used 153 genetic resources of rice to evaluate the effects of the levels of nitrogen application on grain yield and yield-related traits. Significant differences were noted in the yield and yield-related traits of genetic resources between two nitrogen application levels, namely, 4.5 kg/10a (NN: normal nitrogen condition) and 9.0 kg/10a (LN: low-nitrogen condition). Among the tested traits, days to heading (DTH), clum length (CL), grain yield per plant (GYP), number of panicles per plant (NPP), and number of spikelets per panicle (NSP) decreased by 1.8 to 17.9% when the nitrogen application levels decreased from NN to LN. The 1,000-grain weight (TWG) and percentage of ripened grain (PRG) increased by 2.6 to 11.2% under these conditions. Based on nitrogen application levels, two-way analysis of variance (ANOVA) demonstrated significant differences in GYP, NPP, and PRG but not in NSP and TGW. NPP exhibited negative correlations with NSP (-0.44) and TGW (-0.44), and TGW displayed a negative correlation with PRG (-0.34), whereas, GYP exhibited a positive correlation with PRG (0.37) and NSP (0.38). A similar pattern was recorded under the LN condition. NPP, TGW, and PRG were clustered as PA (principle axis) 1 under the LN condition by factor analysis. NSP and GYP were clustered as PA (principle axis) 2. These results demonstrated NPP and NSP as the primary factors contributing to the decrease in grain yield under LN conditions. In conclusion, we selected eight genetic resources that exhibited higher GYP under both NN and LN conditions with higher NPP or NSP. These genetic resources can be considered valuable breeding materials for the adaptation of plants to nitrogen deficiency.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.887-894
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• 2011

To evaluate the effects of abiotic factors of paddy fields on greenhouse gases (GHGs) emissions from rice paddy fields, $CH_4$ emission amounts were investigated from rice paddy fields by different soil organic matter contents, paddy types, and agricultural climatic zone in Yeongnam area during 3 years. $CH_4$ emission amounts according to soil organic matter contents in paddy field were conducted at having different contents of 5 soil organic matters fields (23.6, 28.7, 31.0, 34.5, and $38.0g\;kg^{-1}$), The highest $CH_4$ emission amount was recorded in the highest soil organic matters plot of $38.0g\;kg^{-1}$. High correlation coefficient (r=$0.963^{**}$) was obtained between $CH_4$ emissions from paddy fields and their soil organic matter contents. According to paddy field types, $CH_4$ emission amounts were investigated at 4 different paddy fields as wet paddy, sandy paddy, immature paddy, and mature paddy. The highest $CH_4$ emissions was recorded in wet paddy (100%) and followed as immature paddy 64.0%, mature paddy 46.8%, and sandy paddy 23.8%, respectively. For the effects of temperature on $CH_4$ emissions from paddy fields, 4 agricultural climatic zones were investigated, which were Yeongnam inland zone (YIZ), eastern coast of central zone (ECZ), plain area of Yeongnam inland mountainous zone (PMZ), and mountainous area of Yeongnam inland mountainous zone (MMZ). The order of $CH_4$ emission amounts from paddy fields by agricultural climatic zone were YIZ (100%) > ECZ (94.6%) > PMZ (91.6%) > MMZ (78.9%). The regression equation between $CH_4$ emission amounts from paddy fields and average air temperature of Jul. to Sep. of agricultural climatic zone was y = 389.7x-4,287 (x means average temperature of Jul. to Sep. of agricultural climatic zone, $R^2=0.906^*$)

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.4
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• pp.230-236
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• 2006

The objective of this study was to evaluate the effects of the application of compost on the availability of micronutrients in lettuce. Micronutrient contents of manure-based composts containing various other source materials were investigated. Total and extractable contents of micronutrients in the composts were analysed. Pots containing soil of relatively low micronutrient levels were treated with 1,000 and $2,000kg\;10a^{-1}$ of compost and used to grow lettuce plants under greenhouse conditions. Fresh and dry weights of lettuce and micronutrient uptake were determined after harvest. Manure-based composts of various other source materials contained very different amounts of total and extractable micronutrients. Total contents of B, Cu, Fe, Mn, Mo and Zn were in the range of 26-42, 27-160, 4,300-9,500, 290-790, 0-0.5 and $140-420mg\;kg^{-1}$, respectively. The contents of 0.1 N HCl extractable B, Cu, Fe, Mn and Zn were 23-32, 1.3-2.6, <1, 7-32 and 0.5-5% of total content, respectively. Contents of micronutrients extractable in DTPA solution were generally higher than those extractable in 0.1 N HCl. It was found that the fresh and dry matter productions of the plants were significantly higher in the compost treatment of $2,000kg\;10a^{-1}$. Lettuce grown in soil treated 1,000 and $2,000kg\;10a^{-1}$ of manure-based compost contained higher levels of B, Cu, Mo and Zn than lettuce grown without compost application. However, contents of Fe and Mn in lettuce were relatively lower in the compost treatments. In the compost treatments the proportions of micronutrients in soil and plant were all in the optimum ranges and below the toxicity levels. The results obtained allow us to establish that commercial composts could be used as soil amendment for plastic film house crop production with sufficient supply of micronutrients.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.237-243
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• 2013

Pesticides were sprayed on perilla leaf and leafy lettuce in a greenhouse and the reduction rate of pesticide residues on each vegetable by washing were tested. The reduction rate of pesticide residues by washing for 30 sec~3 min on perilla leaf were 3~63% in tap water, 2~58% in salt water, 6~74% in green tea water, and 8~86% in detergent solution. The detergent solution only showed significant difference in reduction rates compared to the tab water washing. Considering reduction effects of the washing duration, it was showed that the reduction rates were a pattern of inclining as the duration of washing process increased, but there was no significant difference in the reduction rates except the reduction rates between washing in the detergent solution for 1 min and 3 min. Comparing washing in flowing tab water and in stagnant tab water with leafy lettuce, the reduction rate by one time washing were 8~68% in flowing tab water and 7~64% in stagnant tab water. The water and the time used in this experiment were 17.5 L, 2.9 min with flowing tab water and 4 L, 1 min with stagnant tab water. The reduction rate by 3 times washing in stagnant tab water were 16.5~76.6%, and the water and the time used were 12 L, 3 min. Therefore, when the water and the time used to wash vegetables were considered, washing two or three times in stagnant tab water could be more effective than washing one time in flowing tab water.