• Preventive Nutrition and Food Science
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• 제21권2호
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• pp.97-103
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• 2016

In our previous study, an early-maturing variety of rice (Oryza sativa L.), Jinbu can have feature with unique green color, various phytochemicals as well as nutritive components by the optimal early harvesting, called Green Rice$^{(R)}$ (GR). The aims of the present field experiments were to evaluate the changes in the weight of 1,000 kernels, yield, and contents of proximate and bioactive compounds in Chuchung, a mid-late maturing variety, during the pre-harvest maturation of rough rice and to research the appropriate harvest time and potent bioactivity of Chuchung GR. The weights of 1,000 kernels of Chuchung GR dramatically increased until 27 days after heading (DAH). The yields of Chuchung GR declined after 27 DAH and significantly declined to 0.0% after 45 DAH. The caloric value and total mineral contents were higher in the GR than in the full ripe stage, the brown rice (BR). In the GR, the contents of bioactive compounds, such as ${\gamma}$-aminobutyric acid, ${\gamma}$-oryzanol, and ${\alpha}$-tocopherol, were much higher (P<0.05) than those in the BR, specifically during 24~27 DAH. Therefore, bioactive Chuchung GR can be produced with a reasonable yield at 24~27 DAH and it could be useful for applications in various nutritive and functional food products.

• The Plant Pathology Journal
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• 제38권6호
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• pp.679-684
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• 2022

Rice blast is one of the most destructive diseases of rice worldwide, and the causative agent is the filamentous ascomycete Magnaporthe oryzae. With the successful cloning of more and more avirulence genes from M. oryzae, the direct extraction of M. oryzae genomic DNA from infected rice tissue would be useful alternative for rapid monitoring of changes of avirulence genes without isolation and cultivation of the pathogen. In this study, a fast, low-cost and reliable method for DNA preparation of M. oryzae from a small piece of infected single rice leaf or neck lesion was established. This single step method only required 10 min for DNA preparation and conventional chemical reagents commonly found in the laboratory. The AvrPik and AvrPi9 genes were successfully amplified with the prepared DNA. The expected DNA fragments from 570 bp to 1,139 bp could be amplified even three months after DNA preparation. This method was also suitable for DNA preparation from M. oryzae strains stored on the filter paper. All together these results indicate that the DNA preparation method established in this study is reliable, and could meet the basic needs for polymerase chain reaction-based analysis of M. oryzae.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.75-82
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• 2008

Functional stay-green is a beneficial trait that may increase grain yield through the sustained photosynthetic competence during monocarpic senescence in cereal crops. The temporal changes of photosynthesis and related characteristics throughout the grain filling period of a stay-green japonica rice "SNU-SG1" was compared in growth chamber conditions with three high-yielding cultivars(HYVs) and their $F_1$ hybrids with SNU-SG1. SNU-SG1 exhibited a typical characteristic of functional stay-green in terms of chlorophyll degradation and photosynthetic competence during grain filling. According to the photosynthesis-light response curve measured at 10 and 35 d after heading for the flag leaf, SNU-SG1 exhibited higher initial light conversion efficiency and thus higher gross photosynthetic rate at light saturation compared to HYVs. Light saturation point was not different among genotypes, ranging from 1000 to 1500 ${\mu}mol$ photon $m^{-2}s^{-1}$. Net photosynthetic rate at light saturation($P_{max}$) of the upper four leaves in SNU-SG1 was much higher and sustained longer throughout grain-filling than HYVs and $F_1$ hybrids. The sustained high photosynthetic competence of SNU-SG1 during grain filling was ascribed to the longer maintenance of high mesophyll conductance that resulted from not only high chlorophyll content and its delayed degradation but also the slow degeneration of photosystem II(PS II) as judged by chlorophyll fluorescence($F_v/F_m$) of flag leaves. $F_1$ hybrids showed slow degeneration of photosystem II similar to the male parent SNU-SG1 while chlorophyll degradation pattern close to female parents, thus exhibiting a little higher $P_{max}$ than female parents. These results suggest that SNU-SG1 has a typical functional stay-green trait that can be utilized for increasing rice yield potential through the improved dry matter production during grain filling.

• 한국토양비료학회지
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• 제44권2호
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• pp.221-227
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• 2011

논 토양에서 청보리, 자운영과 트리티케일을 시용하고 벼생육초기에 토양 미생물 생태계의 변화에 미치는 영향을 검토하였다. 녹비를 시용한 처리구는 관행에 비해 토양 유기물 함량은 유의적으로 증가하였으나 토양의 나트륨 함량은 유의적인 감소를 나타냈다. 내생균근균의 함량과 구성비율은 녹비 시용으로 관행 보다 유의적으로 높아졌으며 특히 청보리 처리구가 가장 높았다. 또한 토양의 그람음성 세균과 그람양성 세균의 비율과 불포화지방산과 포화지방산 함량의 비율은 청보리 처리구가 다른 처리구에 비해 유의적인 증가를 보였다. 그리고 주성분 분석에서 PC 1에 가장 큰 영향을 미친 미생물 군집은 그람음성 세균인 것으로 나타났으며 청보리 처리구는 관행과 가장 큰 차이를 나타냈다. 이러한 결과를 종합한 결과 청보리는 논 토양의 초기 미생물 다양성을 증대시킬 수 있는 최적의 녹비작물로 판단된다.

• 한국토양비료학회지
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• 제46권6호
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• pp.606-609
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• 2013

To investigate the effect of partial tillage seeding of hairy vetch on green manure biomass and rice yield, on-farm experiment was conducted at eco-friendly hairy vetch cultivation area located in Yesan-gun, Chungcheongnam-do. Seeding methods of hairy vetch consisted of partial tillage seeding (PTS) and broadcasting before rice harvesting (BBRH). Hairy vetch was incorporated into soil on May 18 and rice seedling was transplanted on May 26. The growths of hairy vetch before overwintering were investigated on November 11. Plant height of BBRH plot was longer than that of PTS plot, but somewhat larger number of seedling stand was found in PTS. Biomass and N production of hairy vetch were investigated on May 18. Results of the investigation showed no difference between two seeding methods. Although the seeding time was 21 days late in PTS, the biomass of hairy vetch and rice yield were equivalent to those of BBRH seeding, so we conclude that PTS could improve overwintering survival of hairy vetch in rice cropping system.

• 한국토양비료학회지
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• 제50권3호
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• pp.187-194
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• 2017

This study was conducted to investigate rice yield and soil chemical properties affected by incorporation levels of green barley (GB) and hairy vetch (HV). The GB and HV were applied to the pots at 500, 1,000, 1,500, and $2,000kg\;10^{-1}$ (GB500, GB1000, GB1500, GB2000, HV500, HV1000, HV1500, and HV2000, respectively), and inorganic fertilizer ($N-P-K=9-4.5-5.7kg\;10a^{-1}$) treatment as control. After rice harvesting, chemical properties of soil such as pH, OM, T-N, and available $P_2O_5$ in GB and HV treated treatments were improved over those in Control treatment. The rice yield ranged from 433 to $512kg\;10a^{-1}$ for GB treatments and 490 to $532kg\;10a^{-1}$ for HV treatments, indicating that rice yield was affected by incorporation levels of GB and HV. The rice yields in GB2000, HV1000, HV1500 and HV2000 treatments increased by 3.3, 3.1, 6.4 and 7.4% compared with Control treatment, respectively. Therefore, minimum incorporation level of GB and HV for increasing rice yield was $2,000kg\;10a^{-1}$ of GB and $1,000kg\;10a^{-1}$ of HV.

• 한국토양비료학회지
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• 제35권3호
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• pp.169-174
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• 2002

본 시험은 겨울철 논에 헤어리벳치를 재배후 토양에 환원시키는 방법과 헤어리벳치 환원후 벼를 재배하였을 때 생육 및 쌀 수량에 미치는 영향을 구명하고자 1999년부터 2001년까지 3년간 작물시험장 논에서 수행되었으며, 그 결과는 다음과 같다. 헤어리벳치의 환원시기별 쌀 수량은 이앙 1주전 환원에 비하여 이앙 2~3 주전 환원시 7% 증가되었으며, 화학 질소비료를 무시용하고 벼를 재배할 수 있는 헤어리벳치의 적정 환원량은 $15{\sim}20ton\;ha^{-1}$이었고, 환원량이 $25ton\;ha^{-1}$일 경우 도복이 심하여 안전재배가 우려되었다. 헤어리벳치를 $20ton\;ha^{-1}$환원시 관행(질소 $110kg\;ha^{-1}$)에 비하여 $m^2$당 수수가 20% 증가되어 수량이 6% 정도 증가되었으며, 백미의 완전립 비율은 관행과 유사하였다.

• Molecules and Cells
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• 제24권1호
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• pp.83-94
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• 2007

During monocarpic senescence in higher plants, functional stay-green delays leaf yellowing, maintaining photosynthetic competence, whereas nonfunctional stay-green retains leaf greenness without sustaining photosynthetic activity. Thus, functional stay-green is considered a beneficial trait that can increase grain yield in cereal crops. A stay-green japonica rice 'SNU-SG1' had a good seed-setting rate and grain yield, indicating the presence of a functional stay-green genotype. SNU-SG1 was crossed with two regular cultivars to determine the inheritance mode and identify major QTLs conferring stay-green in SNU-SG1. For QTL analysis, linkage maps with 100 and 116 DNA marker loci were constructed using selective genotyping with $F_2$ and RIL (recombinant inbred line) populations, respectively. Molecular marker-based QTL analyses with both populations revealed that the functional stay-green phenotype of SNU-SG1 is regulated by several major QTLs accounting for a large portion of the genetic variation. Three main-effect QTLs located on chromosomes 7 and 9 were detected in both populations and a number of epistatic-effect QTLs were also found. The amount of variation explained by several digenic interactions was larger than that explained by main-effect QTLs. Two main-effect QTLs on chromosome 9 can be considered the target loci that most influence the functional stay-green in SNU-SG1. The functional stay-green QTLs may help develop low-input high-yielding rice cultivars by QTL-marker-assisted breeding with SNU-SG1.

• 한국작물학회지
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• 제57권2호
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• pp.182-187
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• 2012

본 연구는 이앙재배시 유기물시용이 벼 생육, 수량과 잡초 발생에 미치는 영향을 구명하기 위해 조생종인 조생흑찰을 시험포장에서 수행하였다. 벼의 초장은 이앙 후 50일부터 관행구, 유박처리구에서 비슷한 수준이었으며, 쌀겨처리구에서 전 기간 동안 가장 억제되었다. 벼의 간장과 수장은 관행구와 비교하여 모든 유기물 처리구에서 비슷하게 나타났으며, 유기물 처리구 내에서는 유박처리구에서 가장 컷으며, 쌀겨 처리구에서 가장 작았다. 벼 수량은 관행구와 비교하여 호밀 녹비+쌀겨처리구와 유박처리구에서 각각 관행의 98%, 91%로 수치는 낮았지만 통계적으로 비슷한 수준이었다. 이앙 후 40일경 잡초 발생량은 화학적 방제를 실시한 관행구에서 현저히 적었으며, 유기물처리구에서는 쌀겨처리구에서 다른 유기물처리구보다 현저히 적었다. 또한 쌀겨를 병행 시용한 처리구(호밀녹비+쌀겨)에서 잡초의 발생량이 적었으며, 유박처리구에서 현저히 많은 것으로 나타났다.

• 한국작물학회지
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• 제47권2호
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• pp.137-141
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• 2002

Decomposition of green manure is necessary for the nutrient supply in farm soil. Hairy vetch as a green manure is superior to other winter legumes in terms of wintering ability and N accumulation. This experiment was carried out to investigate the decomposition and N release of hairy vetch and its effect on rice production as the following crop in paddy field. Decomposition of hairy vetch placed by soil depth of 0, 10 and 20cm at transplanting time was investigated by mesh bag method, which was enclosed chopped residue in mesh bags. The fate of $^{15}$ N derived from $^{15}$ N-labeled hairy vetch was investigated at harvest in three levels of N fertilization. Grain yield of the transplanted paddy rice cultured with hairy vetch as starter N were compared with that of applying urea as starter N in the field. Hairy vetch residue decomposed very rapidly both in transplanted and dry-seeded paddy field. In transplanted paddy field, hairy vetch residue lost 72-81 % and 86-90% of its weight after one and five month, respectively, as affected by incorporation depth. The C/N ratio of the decomposing vetch residue increased sharply during the early stages and after then, decreased slowly. The amounts of N and P released from the vetch were about 90% and 97% of initial content after one month, respectively. Recoveries of hairy vetch-$^{15}$ N by rice plant were 30.6, 34.6 and 35.7% in 0, 6 and 12 kg urea-N 10 $a^{-l}$ application, respectively, indicating that N fertilization increased the recovery of hairy vetch. $^{15}$ N. Hairy vetch residue incorporated as starter maintained significant N $H_4$$^{+}$-N concentration in soil water of plow layer until effective tillering stage. Grain yield in the plot applied with hairy vetch was not significantly different from that in the plot with urea. We concluded that hairy vetch incorporation could substitute starter N fertilization and showed possibility to reduce N amount of top-dressing.g.g.