• Applied Microscopy
• /
• v.34 no.3
• /
• pp.199-209
• /
• 2004

The migut epitheluim of the last instar larva in the mosquito larvae, Anopheles sinensis was observed with electron microscopes. The midgut epitheluim of the mosquito larva is composed of a single-layered columnar absorptive cells, regenerative cells and secretory granular cells. The free surface of the columnar absorptive cells has a regular array of microvilli 'brush border', while cell membranes close to the basal lamina are extrmely infolded and a lot of mitochondria are concentrated in those processes. The columnar absorptive cells also contain cell organelles expected to be found in absorptive cell. Midgut regenerative cells which are positioned basally in the epithelium form the groups, which are called 'nidi', composed of 1 or $2{\sim}3$ cells, they show darker appearance than the columnar absoptive cells. The secretory granular cells contain numerous electron dense granules, $200{\sim}400$ nm in diameter. The cone shaped secretory granular cells are located in the basal portion of the midgut epitheluim. The epithelium is surrounded by the subepithelial space and muscle bundles. The subepithelial space, which is filled with fibrous connective tissue, is innervated by many axon cells and tracheoles.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2011.06a
• /
• pp.1231-1232
• /
• 2011

• Applied Microscopy
• /
• v.24 no.1
• /
• pp.1-10
• /
• 1994

The integumental secretory structure is exocrine unicellular gland located in the epidermis of goldfish, Carassius auratus, and divided into two groups, mucous and granular cells. By the histochemical studies of integumental secretions the mucos cells reacted for acidic polysaccharides, and the granular cells for neutral glycoprotein. According to concentration of the secretion the integumental mucous are gradually sulphated. The mucous cells are typical form of goblet cell located in the upper region of the epidermis, and membrane bounded vesicles of the mucous are observed several size and electron densities by the cellular differentiation. The granular cells in middle and lower epidermis are present syncitial forms occasionally, and contain electron dense granules sized $1.0{\mu}m$ which are accumulated in cytoplasmic process held the cells to the basal lamina. The precursors of the integumental secretory materials are originated from the rough endoplasmic reticulum and next transported through the Golgi apparatus as a form of membrane bounded vesicles. After accomplish this process mature secretions are extruded to integumental surface by the mechanism of merocrine secretion in response to nerve stimulations respectively.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2002.08a
• /
• pp.583-585
• /
• 2002

The effects of phosphor properties such as the granular sizes and shape on the crosssectional shape of phosphor layer and plasma discharge characteristics for improving the luminance and luminous efficiency in ac PDP have been investigated. As the granular size decreases, the thickness of vertical side of barrier rib in blue cell decreases, and wheras the thickness of bottom side increases due to increased dispersibility. In addition, the phosphor with round granular shape showing good dispersibility shows better voltage margin and higher luminous efficiency due to their improved discharge volume and packing density.

• The Korean Journal of Cytopathology
• /
• v.19 no.2
• /
• pp.200-205
• /
• 2008

Hibernoma is a rare, benign adipose tumor composed of brown fat cells with eosinophilic granular or multivacuolated cytoplasm. The cytologic features of hibernoma have been rarely reported and may mimic other polygonal cell neoplasms. We report the imprint cytologic features of a case of hibernoma in the left thigh of a 68-year-old woman. Microscopic examination showed large, round, or polygonal brown fat cells. The cells were arranged in fragments or clusters. The nuclei were uniformly round with finely granular chrornatin. The cytoplasm was multivacuolated or univacuolated. The abundant eosinophilic granular cytoplasm was also present. No nuclear atypia were present. Immunohistochemical staining showed that cells were positive for S-100 protein.

• International Journal of Oral Biology
• /
• v.43 no.2
• /
• pp.93-100
• /
• 2018

The investigation of the embryonic development of the cerebellum has a long history. The postnatal normal development of the cerebellum in rodents and other animals became a popular topic for morphological investigations nearly a century ago. However, surprisingly, only a few studies are available regarding the prenatal normal development of the rodent cerebellum, especially in guinea pigs. Cell proliferation is essential for the development of the nervous system. The assessment of cell proliferation can be achieved by using various methods. In this study, we investigated the cell proliferation of the cerebellar cortex in guinea pigs at different stages of pregnancy and in postnatal life. Fetuses were obtained by cesarean section at 50 or 60 days of gestation (dg). Immunohistochemistry was performed with proliferating cell nuclear antigen (PCNA) antibody in the cerebellum. Strong PCNA immunoreactivity was observed in the external granular layer (EGL), which is a neurogenic zone in the cerebellum. The proportion of PCNA-IR cells was greater at 1 week than at 60 dg in lobule I, but not lobule VIII. After 50 dg, the width of the EGL continued to decline until 1 week, due to the maturation of the EGL cells. These results demonstrate the pattern of PCNA immunoreactivity in the developing cerebellum of guinea pigs. This serves as a guideline to study abnormal cerebellum development.

• Journal of Bio-Environment Control
• /
• v.16 no.1
• /
• pp.62-66
• /
• 2007

This study was carried out to develop the stable plug seedling production for hydroponics of spinaches by various nursery media, plug tray size and seed number per plug tray cell. When plant grown in various nursery media, the seeding stand rate was shown in order of granular rockwool with good water retention, granular rockwool>granular rockwool mixed with pearlite>cocopeat>pearlite>poly urethane foame. Thus, poly urethane foame indicated the lower seedling stand rate. There was no difference in growth of the seedlings md the seeding stand rate by the plug tray size, and no significant difference in the plant height and the number of leaves among the seed number per plug tray cell. But, leaf area of plant in 2 grains seeding per cell was $113.0cm^2$, was wider in compared with 5 grains seeding of which leaf area was $88.0cm^2$. Accordingly, the leaf area per plant decreased as more and more the number of seeds per plug tray cell increased. The fresh weight of a plant per plug tray cell was the heaviest with 12.5g in the 2 grains, and the total fresh weight of plants per cell was 33.9g in 4 grains seeding, thus it tended to was bigger compared with other treatments. Consequently, given that the number of seeds per cell was decreased, the fresh weight of a plant increased. On the other hand, the total fresh weight per cell showed a tendency to be reducing as more and more the number of seeds per plug tray cell decreased. The yield in the 4 grains seeding was increased by 46% as $14,910kg{\cdot}ha^{-1}$ in compared with the yield in 2 grains seeding as $10,200kg{\cdot}ha^{-1}$.

• Proceedings of the KSR Conference
• /
• 2010.06a
• /
• pp.640-649
• /
• 2010

Coarse granular materials such as gravel and crushed stone have been used as an important fill materials to large soil structure of railway, road, dam and so on. Although much studies for general soil materials have been carried out domestically, the studies for coarse materials were insufficient. Particularly, it is the level in which the study for dynamic properties(Elastic modulus and damping ratio) of coarse materials, applies the foreign country literature. This is due to the lack of large equipment for element test. But large soil structures made of coarse granular materials are generally important infrastructures. Therefore, the reliable design parameters for coarse materials should be obtained for safe and economic design, construction and maintenance. Triaxial test is the laboratory test method that is capable of controlling a confining pressure and boundary condition. In this project, we made a multi-purpose large triaxial testing system. This testing system is able to test coarse granular materials with maximum particle diameter of 100mm and support both the load control and displacement control. The load cell is installed inside of triaxial cell and the axial displacement is measured locally in order to control and measure more accurately in the small strain level. The verification test of this testing system was carried out with urethane verification specimens. So, from now on the useful information for coarse granular materials are expected to suggested by performing many tests with various material and condition.

• The Korean Journal of Cytopathology
• /
• v.13 no.2
• /
• pp.78-83
• /
• 2002

We report the cytologic features of a case of primary small cell carcinoma of the urinary bladder with high grade transitional cell and signet ring cell carcinomatous components. A 64-year-old male presented with gross hematuria for one week. Computed tomography revealed an ill-defined mass in the left lateral wall of the urinary bladder. Urinary cytology showed hypercellularity with predominantly isolated single cells and clustered cells. They have scanty cytoplasm and naked hyperchromatic nuclei with finely granular nuclear chromatin and rare nucleoli. The tumor cells occurred predominantly singe cells, but a few in clusters. Nuclear molding was prominent. No glandular formation or nesting was noted. The second tumor cells had high nuclear/cytoplasmic ratio, irregular nuclear membrane, and coarse granular chromatin. The background was inflamed and necrotic. The histoiogic findings of transurethral resection were mainly composed of small cell carcinoma, and partly transitional cell and signet ring cell carcinomatous components. Small cell neuroendocrine carcinoma have distinctive cytologic features to make a proper diagnosis.

• The Korean Journal of Zoology
• /
• v.31 no.2
• /
• pp.122-132
• /
• 1988

Ultrastructure of the cutaneous granular glands and production of their secretory granules in the water toad, Bufo steinegeri Schmidt, are studied with light and electron microscopes. Cutaneous granular glands of the water toad have gland cavity in dermis and gland duct in epidermis. Each gland cavity of the granular glands is consisted of 3 types of cells which are inner glandular epithelial cells, outermost myoepithelial cells and another kind of epithelial cells near the gland duct. Characteristically, cytoplasms of the glandular epitelial cells appeared multinucleated masses without differentiation into cells. Poisonous secretory graules excreated by the merocrine secretion are basicafly composed of 2 kinds of granules which are electron dense granules and electron lucent granules. These granules are fused each other and forming compounded structures. According to the granular size and differentiated levels, they are subdivided into 4 types of granules. Synthesis of these secretory granules is occurred at the smooth endoplasmic reticulums of the glandular epithelial cells and limiting membranes of these granules are also originated from these cell organelles.