• Title/Summary/Keyword: Gradient 방법

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Study on HPLC conditions for chemotaxonomy of Ganoderma species (영지버섯의 화학적 계통분류를 위한 HPLC분석 방법에 관한 연구)

  • Yoon, Dae-Eun;Park, Young-Jin;Kwon, O-Chul;Nam, Jae-Young;Kim, Hong-Il;Yoo, Young-Bok;Kong, Won-Sik;Lee, Chang-Soo
    • Journal of Mushroom
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    • v.11 no.2
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    • pp.107-110
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    • 2013
  • This study was carried out to optimize the conditions for a chemotaxonomic classification of Ganoderma species. The mycelia of Ganoderma species were extracted with 100% MeOH, and the concentrated extracts were further purified and partitioned with column chromatography (HP20) and n-BuOH, respectively. From the result of high-performance liquid chromatography (HPLC), the constituents of the samples were efficiently separated with a Chemco Pak $C_{18}$ column ($250mm{\times}4.6mm$) by linear gradient elution using water and acetonitrile as mobile phase components at a flow rate of 0.8 ml/min and detector wavelength at 210 nm. However, the samples without purification or partition were not detected the characteristic peaks. This profile could be used to classify and identify the various Ganoderma species.

Landslide Risk Assessment in Inje Using Logistic Regression Model (로지스틱 회귀분석을 이용한 인제군 산사태지역의 위험도 평가)

  • Lee, Hwan-Gil;Kim, Gi-Hong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.3
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    • pp.313-321
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    • 2012
  • Korea has been continuously affected by landslides, as 70% of the land is covered by mountains and most of annual rainfall concentrates between June and September. Recently, abrupt climate change affects the increase of landslide occurrence. Gangwon region is especially suffered by landslide damages, because the most of the part is mountainous, steep, and having shallow soil. In this study, a landslide risk assessment model was developed by applying logistic regression to the various data of Duksan-ri, Inje-eup, Inje-gun, Gangwon-do, which has suffered massive landslide triggered by heavy rain in July 2006. The information collected from field investigation and aerial photos right after the landslide of study area were stored in GIS DB for analysis. Slope gradient entered in two ways-as categorical variable and as linear variable. Error matrix for each case was made, and developed model showed the classification accuracy of 81.4% and 81.9%, respectively.

Improved Adhesion Strength of the Gradient Zn-Mg Coating on TRIP Steel (TRIP강에서 박막 깊이의 Mg 함량의 변화에 따른 밀착력에 관한 연구)

  • Song, Myeon-Gyu;Kim, Hoe-Geun;Lee, Sang-Yul
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.136-136
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    • 2018
  • 듀얼 페이즈(DP) 강철과 같은 고강도강(HSS), TRIP강, TWIP강은 무게를 줄이고 자동차의 안전을 향상시키기 위해서 자동차 산업에서 광범위하게 쓰이고 있다. HSS강의 내부식성을 향상시키기 위해서, 향상된 박막 재료와 기존의 아연도금 공정을 대체하는 공정 방법이 필요하다. Zn-Mg 박막은 강의 부식 방지에 대한 강력한 후보이며, 물리적 기상 증착 공정(PVD)은 강의 Zn-Mg 박막의 증착을 위한 유망한 공정이다. 그러나 이전 연구에서 보고 된 바와 같이 Zn-Mg 박막은 Zn-Mg 박막의 높은 취성으로 인하여 Zn 박막에 비하여 접착력이 매우 불충분하였다. 따라서 본 연구에서는 Zn-Mg 박막의 첩착력을 향상시키기 위해 TRIP강 기판 위에 증발 증착법을 활용하여 Zn-Mg/Zn 이중층 박막을 합성하고 진공에서 열처리를 실시하여 박막의 깊이에 따른 Mg의 함량 변화를 유도하였다. Zn-Mg/Zn 박막 합성 시 EMH-PVD를 활용하여 증착하였으며, Zn 중간층을 모재와 Zn-Mg 층 사이에 증착하고 진공중에서 열처리를 실시하여 박막 내에서 Mg 함량의 기울기 변화가 박막의 밀착력에 주는 영향을 평가하였다. 증착된 박막은 FE-SEM을 통하여 미세조직과 박막의 두께를 분석하였고 line-EDS를 통하여 깊이에 따른 Zn와 Mg의 변화를 분석하였으며 XRD를 사용하여 합금상을 분석하였다. Lap shear test를 활용하여 박막의 밀착력을 정략적으로 분석하였다. FE-SEM 및 EDS 분석 결과 Zn-Mg/Zn 박막을 진공에서 열처리를 실시한 후 FE-SEM으로 분석한 결과 미세조직의 큰 차이는 보이지 않았으나, line-EDS 결과 Mg이 확산되는 것을 확인 할 수 있었다. XRD 분석 결과 확산한 Mg에 의해서 Zn상은 감소하고 $Mg_2Zn_{11}$, $MgZn_2$와 같은 합금상은 증가하는 것을 확인하였다. Lap shear test 결과 $200^{\circ}C$에서 열처리한 Zn-15wt.%Mg/Zn 박막의 경우 밀착력이 19 MPa로 열처리를 하지 않았을 경우(11 MPa)보다 향상되는 것을 확인하였다. 이러한 결과는 통하여 Zn-Mg 박막의 두께의 Mg의 함량 변화에 의해서 박막의 밀착력이 변화되는 것을 알 수 있다.

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Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography (역상 고속 액체크로마토그래피를 이용한 인삼 사포닌의 분석)

  • Jeong, Seung-Il;Kim, Choen-Suk;Lee, No-Woon;Choi, Kang-Ju;Lee, Yong-Gu;Kim, Il-Kwang
    • Analytical Science and Technology
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    • v.11 no.6
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    • pp.436-439
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    • 1998
  • Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak $C_{18}$ ODS column ($3.9mm{\times}150mm$, $5{\mu}m$), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf and $Rg_1$ was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.

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Effect of Various Regression Functions on Structural Optimizations Using the Central Composite Method (중심합성법에 의한 구조최적화에서 회귀함수변화의 영향)

  • Park, Jung-Sun;Jeon, Yong-Sung;Im, Jong-Bin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.33 no.1
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    • pp.26-32
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    • 2005
  • In this paper, the effect of various regression models is investigated on structural optimization using the central composite method. Three bar truss and the upper platform of a satellite are optimized using various regression models that are polynomial, exponential and log functions. Response surface method is non-gradient, semi-global, discrete and fast converging in optimization problem. Sampling points are extracted by the design of experiments using the central composite method. Response surface is generated using the various regression functions. Structural analysis for calculating constraints is executed to find static and dynamic responses. From this study, it is verified that the response surface method has advantage in optimum value and computation time in comparison to other optimization methods.

Laplace-domain Waveform Inversion using the Pseudo-Hessian of the Logarithmic Objective Function and the Levenberg-Marquardt Algorithm (로그 목적함수의 유사 헤시안을 이용한 라플라스 영역 파형 역산과 레벤버그-마쿼트 알고리듬)

  • Ha, Wansoo
    • Geophysics and Geophysical Exploration
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    • v.22 no.4
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    • pp.195-201
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    • 2019
  • The logarithmic objective function used in waveform inversion minimizes the logarithmic differences between the observed and modeled data. Laplace-domain waveform inversions usually adopt the logarithmic objective function and the diagonal elements of the pseudo-Hessian for optimization. In this case, we apply the Levenberg-Marquardt algorithm to prevent the diagonal elements of the pseudo-Hessian from being zero or near-zero values. In this study, we analyzed the diagonal elements of the pseudo-Hessian of the logarithmic objective function and showed that there is no zero or near-zero value in the diagonal elements of the pseudo-Hessian for acoustic waveform inversion in the Laplace domain. Accordingly, we do not need to apply the Levenberg-Marquardt algorithm when we regularize the gradient direction using the pseudo-Hessian of the logarithmic objective function. Numerical examples using synthetic and field datasets demonstrate that we can obtain inversion results without applying the Levenberg-Marquardt method.

Effective Nonlinear Filters with Visual Perception Characteristics for Extracting Sketch Features (인간시각 인식특성을 지닌 효율적 비선형 스케치 특징추출 필터)

  • Cho, Sung-Mok;Cho, Ok-Lae
    • Journal of the Korea Society of Computer and Information
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    • v.11 no.1 s.39
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    • pp.139-145
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    • 2006
  • Feature extraction technique in digital images has many applications such as robot vision, medical diagnostic system, and motion video transmission, etc. There are several methods for extracting features in digital images for example nonlinear gradient, nonlinear laplacian, and entropy convolutional filter. However, conventional convolutional filters are usually not efficient to extract features in an image because image feature formation in eyes is more sensitive to dark regions than to bright regions. A few nonlinear filters using difference between arithmetic mean and harmonic mean in a window for extracting sketch features are described in this paper They have some advantages, for example simple computation, dependence on local intensities and less sensitive to small intensity changes in very dark regions. Experimental results demonstrate more successful features extraction than other conventional filters over a wide variety of intensity variations.

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Bacterial Community Diversity Associated with Two Marine Sponges from the South Pacific Ocean based on 16S rDNA-DGGE analysis (남태평양에 서식하는 두 종의 해면 Hyrtios sp.와 Callyspongia sp.의 공생세균 군집의 다양성)

  • Park, Jin-Sook
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.255-261
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    • 2010
  • The bacterial community structure associated with two marine sponges, Hyrtios sp. 604 and Callyspongia sp. 612 collected from the South Pacific Ocean were analyzed by 16S rDNA-denaturing gradient gel electrophoresis (DGGE). The phylogenetic analysis showed that the bacterial community associated with Hyrtios sp. 604 contained diverse bacterial groups such as Chloroflexi, Firmicutes, Cyanobacteria, Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, and Acidobacteria. Callyspongia sp. 612 harbored Chloroflexi, Cyanobacteria, Alphaproteobacteria, and Gammaproteobacteria. Hyrtios sp. 604 belonging to genus Hyrtios known to produce natural products showed greater bacterial diversity than Callyspongia sp. 612. Phylum Actinobacteria was shown to be one of dominant bacterial groups in Hyrtios sp. 604. Although the same phyla of bacteria were found in both sponge species, the spongeassociated predominant bacterial groups differed between the two sponges with different chemical characteristics from the same geographical location. Uncultured bacteria represented over 90% of the bacteria diversity present in all bacterial communities of the sponges.

Myo-Inositol Synthesis in the Milk of Lactating Rats (쥐 우유중의 Myo-Inositol 생성에 관한 연구)

  • Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.19 no.3
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    • pp.121-129
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    • 1976
  • A high concentration of myo-Inositol in rat's milk was observed (61-91mg. of myo-Inositol per 100g of milk) by gas-liquid chromatographic method, using a 3% SE-52 column. Feeding experiments showed that approximately 85% of myo-Inositol in milk was from dietary origin: the rest was considered to be synthesized by 1L-myo-Inositol-1-phosphate lyase. Results suggested that the biosynthesis was not sufficiently high to permit the maintenance of its myo-Inositol level in milk. However, study $using(^{14}C)-glucose$ injection into lactating female rats confirmed biosynthesis of myo-Inositol from glucose in mammary gland. This biosynthesis reached a maximum within an hour after $(^{14}C)-glucose$ injection intraperitoneally as lactose biosynthesis did. Study using $(^3H)-myo-Inositol$ confirmed that most of the myo-Inositol in milk was transported from blood plasma myo-Inositol against a concentration gradient. About four hours after the beginning of the injection of $(^{14}C)-glucose$, the specific radioactivity of myo-Inositol in milk was 8% of that of glucose in the blood. When $(^3H)-myo-Inositol$ was injected, the specific radioactivity of myo-Inositol in milk was about 26% of that of blood six hours after injection.

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Occlusion Processing in Simulation using Improved Object Contour Extraction Algorithm by Neighboring edge Search and MER (이웃 에지 탐색에 의한 개선된 객체 윤곽선 추출 알고리즘과 MER을 이용한 모의훈련에서의 폐색처리)

  • Cha, Jeong-Hee;Kim, Gye-Young;Choi, Hyung-Il
    • Journal of the Korean Institute of Intelligent Systems
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    • v.18 no.2
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    • pp.206-211
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    • 2008
  • Trainee can enhance his perception of and interaction with the real world by displayed virtual objects in simulation using image processing technology. Therefore, it is essential for realistic simulation to determine the occlusion areas of the virtual object produces after registering real image and virtual object exactly. In this paper, we proposed the new method to solve occlusions which happens during virtual target moves according to the simulated route on real image using improved object contour extraction by neighboring edge search and picking algorithm. After we acquire the detailed contour of complex objects by proposed contour extraction algorithm, we extract the three dimensional information of the position happening occlusion by using MER for performance improvement. In the experiment, we compared proposed method with existed method and preyed the effectiveness in the environment which a partial occlusions happens.