• 제목/요약/키워드: Ginsenoside content

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Study on of Extraction Methods of Saponin in Ginseng Products (인삼제품의 Saponin 추출방법에 관한 연구)

  • 손현주;장진규
    • Journal of Ginseng Research
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    • 제8권1호
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    • pp.32-37
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    • 1984
  • In order to shorten the extraction time of saponin in ginseng products, election with Extrelut column and phase-separation methods were compared. The results obtained are as follows. 1. Saponin of ginseng products was extracted completely within 3 hours by election method with Extrelut column, and the rate of removing glucose by the column was increased with increasing glucose content in ginseng products. 2. Stirring method was superior to refluxing method for removing sugars from ginseng products, and removing rate was deceased in the order of lactose, sucrose and glucose. 3. Extraction rate of ginsenoside from ginseng extracts by the elution method was nearly same as that of phase-separation method; however, the former was much higher than the latter in the case of ginseng teas. Therefore, the elution by Extrelut column is to be improper for extraction of saponin in ginseng tea which contains much sugar. It was necessary to remove lipophilic compounds for extraction of saponin from ginseng extracts by elution with Extrelut column.

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Studies on Quality Evaluation of Crude Drug Preparation(II) -Analysis of Saengmaek-san by Thin Layer Chromatography and High Performance Liquid Chromatography- (생약 복합제제의 품질평가에 관한 연구(제 2 보) -생진산(生賑散)의 품질평가 방법에 관하여-)

  • Hong, N.D.;Kim, J.W.;Won, D.W.;Kong, Y.C.;Kim, N.J.;Joo, S.M.
    • Journal of Pharmaceutical Investigation
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    • 제17권1호
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    • pp.22-30
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    • 1987
  • Evaluation method of crude drug preparations was studied in Saengmaek-san. Zig-zag TLC scanning profiles and high performance liquid chromatograms were obtained from Saengmaek-san and its each crude drug. A method using TLC densitometry and high performance liquid chromatography was established for the precise determination of ginsenoside $Rb_1$ in Saengmaek-san containing Ginseng Radix. Consequently, ginsenosicle $Rb_1$ content was 0.45-0.48 mg per g of Saengmaek-san. This method was found to be useful for the quality evaluation of oriental medicinal preparations.

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Saponins in Leaf and Stem of Gynostemma pentaphyllum (돌외 (Gynostemma Pentaphyllum)의 잎과 줄기 사포닌)

  • Park, Hoon;Lee, Mee-Kyung
    • Journal of Ginseng Research
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    • 제8권2호
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    • pp.172-177
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    • 1984
  • Saponins in leaf and stem of Gynostemma pentaphyllum that was collected from Jeju Island were extracted by the method for ginseng saponin. Comparison by overlapping chromatogram(HPLC) of pentaphyllum on that of ginseng and cochromatogram and ginseng although ginsenoside Rg2, Rg1 and Rf might be in common with rare possibility. It seems to be little difference in the kind of saponin glycosides between leaf and stem of pentaphyllum. Saponin content in leaf of pentaphyllum was higher than in stem, and much higher than those in ginseng. The kind of saponin glycoside in pentaphyllum appeared to be less than 22 and greater than those in ginseng. There was almost no change in saponins of pentaphyllum in methanol for 3 years at room temperature.

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Chemical Components of Rusty Root of Ginseng (적변인삼의 화학적 성분에 관한 연구)

  • 이태수;목성균
    • Journal of Ginseng Research
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    • 제19권1호
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    • pp.77-83
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    • 1995
  • This study was conducted to investigate the chemical components of rusty root for seeking the cause of rust. Na, Fe contents were higher in various tissues of six-year-old rusty root than healthy root. Contents of total sugar in epidermis of rusty root was low but crude lignin and suborn contents were high. Crude lipid content increased with the rate of rusty symptoms in each part of root respectively. Pentadecanoic, stearic, oleic, lignoseric acid contents increased in cortex of tap root with the rate of rusty symptoms but decreased in pith. Total ginsenoside and crude saponin contents increased , in pith and cortex of tap root with the rate of rusty symptoms but decreased in lateral and fine root.

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Physicochemical Properties of Freeze Dried Ginseng from the Fresh Ginseng Stored at Low Temperature (저온저장 후 냉동건조한 인삼의 이화학적 특성)

  • 장진규;심기환
    • Journal of Ginseng Research
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    • 제18권1호
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    • pp.60-65
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    • 1994
  • Fresh ginseng of same grade was stored under the 4$\pm$1$^{\circ}C$ and 87~92% RH for 10 weeks. During the storage, an aliquot amount of the ginseng was drawn, freeze dried and chemical constituents and physicochemical parameters were measured. After 10 weeks of storage drying rate and shrinkage of ginseng were 1520% and 9.04%, respectively, mold growth was seen at week 5 and observed for 51.2% of the ginseng week 10. Amylase activity level was elevated at the early stage of storage and decreased to 5% of initial value at week 5. At week 5, the elevated amylase activity was inconcomitant with the appearance of the mold growth. Crude protein contents were increased and decreased, respectively 5 week post storage. No significant changes in crude fat, crude fiber, ash, total sugar, n-butanol extract and ginsenoside were observed. The content of water-extractable substance showed maximum at week 7 to 8. The value of pH was slightly elevated and reducing sugar was increased during the storage. Key words Ginseng storage, physicochemical properties, drying rate, shrinkage, amylase activity.

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Effect of Controlled Atmosphere and Modified Atmosphere Storage on the Apparent Quality and Saponin Component of Fresh and Red Ginseng (CA 및 MA 저장이 수삼 및 홍삼의 외관품질 및 사포인 조성에 미치는 영향)

  • 전병선;성현순
    • Journal of Ginseng Research
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    • 제19권1호
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    • pp.62-72
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    • 1995
  • During the controlled atmosphere storage (CA), fresh ginseng showed good appearance in quality, and other deterioration of freshness was not observed until 12 weeks. On the other hand, MA storage had kept freshness only in treatment of 1 until 8 weeks. There was no significant difference between treated and non-treated sample with preservatives, and not treated sample was not infected with various different fungi. Moisture contents and hardness of ginseng in all treatments were not changed much until 12 weeks, and surface shrinkage did not occur either. But shear stress increased somewhat in all treatments after 12 weeks. The granule of microstructure in tissue diminished slightly. The apparent Quality of red ginseng was good until 4 weeks of treatment. But as time passed, white skin and wrinkled skin were generated and darkened in its color. B-1 in CA and E-1 in MA were found to be the most favorable one. The content of crude saponin did not change significantly during storage of CA or MA by preservation conditions and period. Though a small increase in saponin content from 4.92% to 5.43% was recognized in B-1, which was treated with preservative and 6.0% In B-2, control, this could rather explain increment of soluble component by butanol. Thus, there was no change in total contents of ginsenoside pattern and composition of each content. The Rbl content in B-1 and B-2 were 0.98%, and 0.97%, respectively, whereas that of control was 0.96%. E-1 of MA, treated with preservative was 5.32% after 12 weeks, but was 5.73% in control, indicating that ginsenosides pattern was quite similar to that of CA storage.

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Extracting Conditions for Promoting Ginsenoside Contents and Taste of Red Ginseng Water Extract (홍삼 물 추출액의 사포닌 함량 및 맛의 증진을 위한 추출 조건)

  • Li, Xiangguo;Han, Jin-Soo;Park, Yong-Jun;Kang, Sun-Joo;Kim, Jung-Sun;Nam, Ki-Yeul;Lee, Ki-Teak;Choi, Jae-Eul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • 제54권3호
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    • pp.287-293
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    • 2009
  • In this study, red ginseng extract solutions were analyzed to set up the functional saponin content and quality optimization condition. The highest saponin content among the total red ginseng extracts was 64.6 mg / 100 ml which was extracted at $75^{\circ}C$ for 18 hours. In addition, the saponin content decreased according to the increased extraction temperature and time. The highest total content of $Rb_2$ and Re was 11.8 mg / 100 ml at $75^{\circ}C$ for 12 hours which decreased according to the increased extraction temperature and time. The prosapogenin content of red ginseng extract was increased at $75^{\circ}C$ and $85^{\circ}C$ while the content decreased at $95^{\circ}C$, in which the highest prosapogenin content was 34.9 mg / 100 ml at $85^{\circ}C$ for 24 hours. The total sugar content and cloudness were increased according to the increased extraction time at $95^{\circ}C$, but pH and hue value were decreased according to the increased extracted time. The highest sweetness content was 4.0% which was found at $95^{\circ}C$ for 24 hours extract. Therefore, the most appropriate red ginseng extracting method was lower the temperature for saponin content at first time in combination with raise the temperature for taste at second time.

Changes of Ginsenosides and Physiochemical Properties in Ginseng by New 9 Repetitive Steaming and Drying Process (새로운 자동 구증구포방법에 의한 인삼사포닌의 변환 및 이화학적 특성)

  • Jin, Yan;Kim, Yeon-Ju;Jeon, Ji-Na;Wang, Chao;Min, Jin-Woo;Jung, Sun-Young;Yang, Deok-Chun
    • Korean Journal of Plant Resources
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    • 제25권4호
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    • pp.473-481
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    • 2012
  • This study was conducted to investigate the contents of ginsenosides and physiochemical properties of Panax ginseng after 9 times steaming and drying treatment by using the new auto steamer which is more fast and simple than previous report. In the process of steaming and drying, the content of six major ginsenosides such as Rg1, Re, Rb1, Rc, Rb2 and Rd were gradually decreased. On the other hand, the content of seven minor ginsenosides includes Rh1, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rg3, 20(R)-Rg3, Rk1 and Rg5 were gradually increased. We observed the protopanxadiol ginsenosides such as Rb1, Rb2, Rc and Rd were converted into 20(S)-Rg3, 20(R)-Rg3, Rk1 and Rg5; similarly protopanxatriol ginsenosides of Rg1 and Re were converted into Rh1, 20(S)-Rg2 and 20(R)-Rg2. Based on the result of fresh ginseng, the contents of reducing sugar, acidic polysaccharide and total phenolic compounds were gradually increased and reached to maximum at 7 times repetitive steaming process of the fresh ginseng. Whereas DPPH radical scavenging activities were gradually decreased to 68% at 7 times steaming. New auto 9 repetitive steaming and drying process has similar production with original methods, but content of benzo(a)pyrene were not almost detected comparatively taking less time. The present results suggested that this method is best for the development of value-added ginseng industry related products.

Quality Characteristics of Ginseng Jung Kwa Obtained by Different Sugar Treatments (인삼정과의 제조에 있어 당 종류에 따른 품질학적 특성)

  • Song, Mi-Ran;Kim, Mee-Ree;Kim, Hyun-Ho;Chu, Seok;Lee, Ka-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • 제39권7호
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    • pp.999-1004
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    • 2010
  • This study was carried out to investigate the quality characteristics of ginseng Jung Kwa obtained by treatments with different sugars. Ginseng Jung Kwa was made with four different sugars (sucrose, glucose, honey and fructose). Chemical characteristic was measured by ginsenosides content and rheological characteristics were measured by rheometer and color meter. Total ginsenoside and Rf contents on Ginseng Jung Kwa by sucrose were highest at 10.0, and 7.82 mg/g, respectively. On ginseng Jung Kwa by fructose, total ginsenoside content was the lowest, and hardness and adhesiveness were the highest, and by honey, stiffness, cohesiveness, gumminess, chewiness, and springiness were the highest, and fracture force did not appear as a significant difference by treatment of different sugars. Coefficient of variance on ginseng Jung Kwa by honey was the highest. On ginseng Jung Kwa by sucrose, color (lightness, redness and yellowness) all were the highest. Browning on Jung Kwa by fructose was the greatest. Sensory evaluation preference analysis (color, taste, texture, flavor and overall acceptability) on ginseng Jung Kwa were determined by 50 panelists (30~50's age) using 5-point scale. On ginseng Jung Kwa by honey, texture and overall acceptability were the best. Ginseng Jung Kwa by fructose was very sticking and adhering, so preference by sensory evaluation was inferior to others.