• Journal of Veterinary Science
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• 제22권4호
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• pp.50.1-50.10
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• 2021

Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions: Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.

• 한국자원공학회지
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• 제55권6호
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• pp.546-552
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• 2018

금번 6차 광해방지 국제심포지움 발표사례로 볼 때, 국가별 광산개발의 역사, 지리여건, 경제상황 등이 상이하나, 과거 개발된 광산으로 인해 발생한 산성광산배수로 인한 수질오염, 광물찌꺼기 유실로 인한 토양오염 및 수질오염, 사면파괴로 인한 산사태, 지하갱도 붕괴로 인한 지반침하 등으로 인간의 생활이 위협당하고 있다. 나라별로 기술력과 법제도의 차이가 있고, 광해복구가 진행 중인 나라와 이제 시작하려고 제도마련 및 기술지원을 받아야 하는 나라도 있다. 광해복구를 시작하려는 나라에서는 국제사회에 도움을 요청하고 있어 기술적 제도적으로 앞선 선진국에서는 복구를 시작하려는 나라와 공조를 통해 광해를 복구해야할 필요가 있다.

• ALGAE
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• 제34권3호
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• pp.217-228
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• 2019

The marine brown algal genus Colpomenia has a worldwide distribution, with five species reported in Korea and Japan. However, no studies to date attempted to identify the number of species and geographical distribution of Colpomenia along Chinese coast. To fill the biodiversity knowledge gap, we analyzed 63 mitochondrial cox3 and 62 mitochondrial atp6 sequences of Colpomenia specimens collected from 30 localities along the Chinese coast. Maximum likelihood and Bayesian inference trees suggest the presence of at least three Colpomenia species (i.e., C. peregrina, C. claytoniae, and C. sinuosa) in China. C. peregrina and C. claytoniae are documented for the first time. C. sinuosa was only found in the South China Sea and its distribution didn't overlap with that of C. peregrina which was found in the Yellow-Bohai Sea and the East China Sea. C. claytoniae appears to be confined to three isolated islands in the East and the South China Sea, where it occurs in sympatry with, respectively, C. peregrina and C. sinuosa. Future study can focus on comparing eco-physiological differences of Colpomenia species in response to environmental variables and exploring possible genetic hybridization / introgression at inter-specific contact zones.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.661-668
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• 2020

In Europe, 5 Lipoptena species have been recorded, including Lipoptena fortisetosa. This species, native to Asian countries, was described as a parasite of sika deer and its appearance in Europe dates back to more than 50 years ago. Lipoptena fortisetosa has been recently reported in Italy, sharing its hosts with Lipoptena cervi. A morpho-molecular approach was developed to determine the phylogenetic interrelationship of Italian and Asian CO1 haplotypes sequenced from Lipoptena fly individuals collected in Italy, and their DNA sequences were compared with conspecifics available in GenBank; morphological key-characters (terminalia) of L. fortisetosa were compared with the original description. Two haplotypes were recorded from Italy and assigned to L. cervi and L. fortisetosa, respectively. The latter was part of the monophyletic clade L. fortisetosa, along with 2 Central European and 2 Korean haplotypes (100% identical to one of the Korean haplotypes); moreover, Italian L. fortisetosa female terminalia were consistent with the original description of Asian individuals. Pending more in-depth investigations, this study provides a first answer to the hypothesis of the recent colonization of Italy by L. fortisetosa from Asia as we did not detect any obvious and stable morphological and molecular differences in specimens from the 2 geographical areas. The presence of the sika deer in Europe was retraced and the possible route traveled by the parasite from Asia and the eco-biological factors that may have enhanced its settlement are discussed.

• 대한본초학회지
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• 제29권1호
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• pp.27-33
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• 2014

Objectives : This study was carried out to identify DNA markers of "Allium sativum" be circulated from Korea and China, which is difficult to discriminate from morphological characters because of fragmental materials of bulb. That is, all these studies focused on the discrimination of Allium sativum L. But these day, Chinese A. sativum was in circulated Korean A. sativum in Korean medicine markets. Therefore, the purpose of our study was to develop molecular markers for discrimination between Korean A. sativum and imports from China. Methods : Materials were collected randomly from a markets in Korea and China and be analyzed with matK, ndhF and trnL-F regions of chloroplast DNA (cpDNA). We collected 45 A. sativum individuals from Korean and Chinese medicine markets, in 2013. Results : As a results, matK and ndhF regions of cpDNA was shown to be identify, Species that grow from warm place and cold place can divide as five SNP (Single nucleotide polymorphisms) markers in matK and ndhF genes. Also, in trnL-F regions, found one SNP that can divide Korean A. sativum and Chinese A. sativum. Conclusions : From the analysis of matK and ndhF regions of cpDNA, we presumed that three markers of cpDNA were found by useful marker that can distinguish Korean, Chinese, Warm place type, and Cold place type. Individual differences of Korean and Chinese was thought that appear in geographical difference and genetic difference by environment for long hour even if same species.

• 인간식물환경학회지
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• 제24권6호
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• pp.619-628
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• 2021

Background and objective: Native to the limestone zones of the Korean Peninsula, Zabelia tyaihyonii is a popular plant for landscaping. As it is now classified as a rare species, the conservation of its genetic resources is necessary. Methods: In this study, which aimed to understand the morphological variation of Z. tyaihyonii, 18 characteristics of Z. tyaihyonii from five habitats were examined. Results: Of these 18 characteristics, 16 characteristics showed significant differences among sites, and the coefficient of variation ranged from 5.4% (for corolla lobe number) to 31.3% (for flower number). Notable variations were observed in the size of flower and calyx lobe. When the corolla length and calyx lobe length were used as the classification key of Z. tyaihyonii, the sites were divided into those with small, intermediate, and large values. Hair was observed on the filament of all samples, a finding which conflicts with an earlier report. Rather than classifying Z. tyaihyonii into different species on the basis of corolla length (COL) and calyx lobe length (CALL) values, we recommend modifying the species description to incorporate the variation in these characteristics of interest. Principal component analysis results showed that the first main component was highly correlated with the traits related to the size of the calyx lobe (length: 0.819, width: 0.758), and the second main component was highly correlated with the traits related with the size of the inflorescence (length: 0.790, width: 0.626). Conclusion: Several notable variations were identified among the characteristics related to inflorescence and calyx lobe. There is little genetic exchange among groups, or each group is influenced by micro environmental factors, because sites that are located nearby. In addition, the difference between COL and CALL, which is used as the classification key for Z. tyaihyonii, was divided into small group, large group, and intermediate group, regardless of the sites' geographical distance.

• 한국비블리아학회지
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• 제34권3호
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• pp.89-107
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• 2023

공공도서관은 지역사회 이용자의 다양한 요구를 파악하여 이를 충족시키는 서비스를 기획하고 제공하여야 한다. 도서관 이용자에 대한 이해를 위해서는 도서관 서비스 영역에 대한 파악이 우선될 필요가 있다. 현행의 공공도서관 서비스 영역은 주로 도서관이 위치한 행정구역으로 설정되고 있어, 도서관에 대한 이용자의 실제 접근 패턴을 고려하지 못하는 한계가 있다. 본 연구에서는 도서관이 속한 지역의 교통적 및 지리적 특성과 이용자의 도서관 접근 패턴을 반영한 서비스 영역을 생성하고자 하였다. 구체적으로 7개 특별·광역시의 502개 도서관의 도로 네트워크 데이터를 활용하여 경사도가 반영된 이동 거리와 시간을 파악하였으며, 최단 경로 알고리즘을 적용하여 도보 또는 차량으로 30분 이내 범위의 서비스 영역을 생성하였다. 그 결과 지형적 요소에 따라 도서관별 서비스 영역 양상에 차이가 발생하는 것을 확인하였으며, 이는 직선거리 기반의 서비스 영역과 비교하여 도서관 접근에 대한 현실적 여건을 더욱 잘 반영하는 것으로 판단되었다. 이러한 서비스 영역 생성 방법은 도서관의 이용자의 수, 특성, 요구를 보다 정확하게 이해하는 데 기여할 수 있을 것이다.

• 한국동물학회지
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• 제18권3호
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• pp.141-146
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• 1975

本 硏究는 韓國人集團의 acatalasemia와 hypocatalasemia의 頻度를 조사하고 그 頻度의 分布를 日本人과 中國(대만)人集團의 頻度와 비교검토 하고저 실시하였으며 그 결과는 다음과 같다. 1. 韓國의 南部地方, 全州, 光州, 釜山 및 大邱의 住民 총 3,629명을 조사하였으나 acatalasemia는 1例도 발견되지 않았으며, hypocatalasemia는 全州에서 0.26%, 광주 0%, 부산 0.21%, 및 大邱 0.06%이었다. 2. 韓國의 南部地方에서 얻은 hypocatalasemia 頻度는 일반적으로 中部地方 보다 낮았다. 3. 本 硏究의 결과는 中國(대만)人의 頻度와 비슷하고 日本人 頻度보다는 높았다. 4. hypocatalasemia 頻度의 地域差는 환경에 의한 自然的淘汰로 추측된다. 그리고 日本에 거주하는 韓國人集團에서 頻度가 높은 이유는 (1) 원래 韓國人集團의 頻度가 높고, (2) 在日 韓國人間의 選擇結婚의 영향으로 추측된다.

• Journal of Species Research
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• 제12권4호
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• pp.281-285
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• 2023

The red-tongue viper(Gloydius ussuriensis) is one of only three species of the genus Gloydius found in South Korea. Gloydius ussuriensis has a narrow activity radius and is distributed nationwide, and this species was reported to have the largest population among the Korean species in genus Gloydius. Preliminary results of a phylogenetic analysis using part of the mitochondrial DNA indicated that domestic G. ussuriensis is not comprised of monophyletic groups, and morphological analysis showed differences between domestic populations. In this study, we developed 17 microsatellites for the analysis of G. ussuriensis genetic diversity based on these characteristics. These microsatellites were developed using six multiplex panels, which could be employed to validate 80 G. ussuriensis specimens from different geographical regions in South Korea. The average number of alleles per locus was 12.2 and ranged from 4 to 25 alleles; the observed heterozygosity ranged from 0.238 to 0.950 and the expected heterozygosity ranged from 0.213 to 0.933. As a result of assessing four inland populations, a high level of genetic diversity was confirmed. These newly developed markers will be useful for further studies on the population structure and evolutionary history of the G. ussuriensis.

• Tuberculosis and Respiratory Diseases
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• 제87권3호
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• pp.386-397
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• 2024

Background: Despite the global increase in nontuberculous mycobacterial pulmonary disease (NTM-PD), clinical characteristics show geographical variations. We investigated the clinical characteristics of patients with NTM-PD in South Korea. Methods: We systematically reviewed articles concerning patients with NTM-PD in South Korea until February 2022. Individual participant data, regardless of treatment, were collected using a standard case report form. Results: Data of 6,489 patients from 11 hospitals between 2002 and 2019 were analyzed. The mean age was 61.5±11.7 years, of whom 57.7% were women. Mycobacterium avium (41.4%) and Mycobacterium intracellulare (38.4%) comprised most of the causative species, followed by Mycobacterium abscessus subspecies abscessus (8.6%) and M. abscessus subspecies massiliense (7.8%). Bronchiectasis (59.4%) was the most common pulmonary comorbidity. Although reported cases of NTM-PD increased over the years, the proportions of causative species and radiologic forms remained similar. Distinct clinical characteristics were observed according to age and sex. Men were older at the time of diagnosis (median 63.8 years vs. 59.9 years, p<0.001), and had more cavitary lesions than women (38.8% vs. 21.0%, p<0.001). The older group (≥65 years) had higher proportions of patients with body mass index <18.5 kg/m² (27.4% vs. 18.6%, p<0.001) and cavitary lesions (29.9% vs. 27.6%, p=0.009) than the younger group. Conclusion: We conducted a meta-analysis of the clinical characteristics of patients with NTM-PD in South Korea, and found age- and sex-related differences in disease-specific severity. Further investigation would enhance our comprehension of the nature of the disease, and inherited and acquired host factors.